Labshake search
Citations for Miltenyi Biotec :
4501 - 4550 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... with HBSS buffer with calcium and magnesium and prepared for myelin removal process (Myelin Removal Beads II, #130-096-733; Miltenyi Biotec). Then ...
-
bioRxiv - Neuroscience 2022Quote: ... The gentleMACS™ Dissociator (#130-096-427, Miltenyi Biotec) was used for mechanical dissociation steps following the Neural Tissue Dissociation Kit (P ...
-
bioRxiv - Neuroscience 2022Quote: ... The gentleMACS™ Dissociator with Heaters (#130-096-427; Miltenyi Biotec) was used for mechanical dissociation steps during 30 minutes at 37ºC ...
-
bioRxiv - Neuroscience 2022Quote: ... passed through a MACS column (Miltenyi Biotec). The second (positive ...
-
bioRxiv - Neuroscience 2022Quote: ... The samples were passed through a 45 μm Nitex mesh to remove undissociated cell clumps and after addition of myelin removal beads (Miltenyi Biotec), passed through a MACS column (Miltenyi Biotec) ...
-
bioRxiv - Neuroscience 2022Quote: ... Cell purity was assessed by anti-GLAST (ACSA-1) phycoerythrin antibody (Miltenyi Biotec) using flow cytometry (CyanADP Cytometer ...
-
bioRxiv - Neuroscience 2022Quote: ... Human iPSCs (hiPSCs) were cultured in a chemically defined growth medium (StemMACS iPS-Brew XF (Miltenyi Biotec) or mTSeRTM1 (Stem Cell Technologies ...
-
bioRxiv - Neuroscience 2022Quote: ... mouse anti-GLAST (1: 300, Miltenyi Biotec), mouse anti-caveolin1 (1:50 ...
-
bioRxiv - Neuroscience 2022Quote: ... Anti-O4 selection using magnetic microbeads (Miltenyi Biotec) to separate O4+ immature oligodendrocytes from cell suspensions was also carried out to remove ACSA-1 positive astrocytes contaminated by oligodendrocytes ...
-
bioRxiv - Neuroscience 2022Quote: ... On day five ECs were dissociated with StemPro Accutase (diluted 1:6 in D-PBS) and separated via Magnetic-activated cell sorting (MACS, Miltenyi Biotec) using CD34 MicroBeads ...
-
bioRxiv - Neuroscience 2022Quote: We used a light-sheet microscope (UltraMicroscope II with Infinity Corrected Objective Lenses, Miltenyi Biotec) with an attached camera (Andor Zyla sCMOS) ...
-
bioRxiv - Cell Biology 2022Quote: ... CD8+ T cells were cultured in TexMACS media (Miltenyi Biotec) supplemented with 100 U/ml human IL-2 (Miltenyi Biotec ...
-
bioRxiv - Immunology 2022Quote: ... All lung lobes were harvested into a gentleMACS C tube (Miltenyi Biotec) containing 3 mL of RPMI media with 70 μg / mL of Liberase TM (Roche ...
-
bioRxiv - Cancer Biology 2022Quote: ... we applied a dead cell removal kit (Miltenyi Biotec) to all samples to reach a viability of at least 85 to 90 % ...
-
bioRxiv - Cancer Biology 2022Quote: ... from Miltenyi Biotec [130-095-929] as per manufacturers’ instruction ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tissue pieces were thereafter mechanically disrupted with gentle MACS tube (Miltenyi Biotec) in cold DMEM with 10% FBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... The cells were filtered with a 70 μm cell strainer and dead cell removal was performed on single cell suspensions (Miltenyi Biotec) and live tumor cells were frozen in 90% heat-inactivated fetal bovine serum (HI-FBS ...
-
bioRxiv - Developmental Biology 2022Quote: ... The remaining cells were pelleted and resuspended in 80 µl of MACS buffer (PBS pH 7.2, 0.5% BSA and 2 mM EDTA) and 20 µl anti-CD45 MicroBeads (Miltenyi Biotec #130-052-301) or 20 µl anti-F4/80 MicroBeads (Miltenyi Biotec #130-110-443 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cell suspension was applied onto the pre-balanced LS column (Miltenyi Biotec #130–097-679) that was placed in the magnetic Separator ...
-
bioRxiv - Developmental Biology 2022Quote: ... or 20 µl anti-F4/80 MicroBeads (Miltenyi Biotec #130-110-443) per 107 total cells ...
-
bioRxiv - Developmental Biology 2022Quote: ... Unlabeled cells which passed through the LS column loading CD45 microbeads and LD column (Miltenyi Biotec #130-042-901) retaining weakly labeled cells were defined as the CD45- fraction ...
-
bioRxiv - Developmental Biology 2022Quote: A dissociated single-cell suspension was passed through 30 µm nylon mesh (Pre-Separation Filters; Miltenyi Biotec #130-041-407) to remove cell clumps that could clog the column ...
-
bioRxiv - Developmental Biology 2022Quote: ... filtered through 30 uM and 20 uM filters (Miltenyi Biotec) and cleaned of debris using a Percoll (Sigma ...
-
bioRxiv - Genetics 2022Quote: ... Then cells were incubated with human CD34 Microbeads (Miltenyi Biotec) and Fc receptor blocker in MACS buffer (1% BSA/2mM EDTA/PBS ...
-
bioRxiv - Developmental Biology 2022Quote: ... spheres were dissociated using the MACS EB Dissociation Kit (order no. 130-096-348) according to manufacturer’s instruction (Miltenyi Biotec). The cell pellet was resuspended using Noci-3 medium (see Fig ...
-
bioRxiv - Developmental Biology 2022Quote: ... and dissociated into single-cell suspensions using Neural Tissue Dissociation kit (P) (Miltenyi Biotec, Germany), following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... Samples were further subjected to mechanical disruption by gentleMACS™ Octo Dissociator (Miltenyi Biotec) and filtration through fine steel mesh (40um ...
-
bioRxiv - Developmental Biology 2022Quote: ... Remaining cells were incubated with CD31 micro beads (Miltenyi Biotec) at 4°C for 15 mins to enrich EC ...
-
bioRxiv - Developmental Biology 2022Quote: ... The media was further supplemented with 5µM CHIR99021 (StemMACS, 130-103-926) on D2 and with or without 50 ng/ml hGdf11 (130-105-776, Miltenyi Biotec) on D3 ...
-
bioRxiv - Developmental Biology 2022Quote: ... supplemented with 10 ng/ml hFGF-2 (Miltenyi Biotec, 130-104-925). The media was further supplemented with 5µM CHIR99021 (StemMACS ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were incubated with CD45 micro beads (Miltenyi Biotec) at 4°C for 15 mins to deplete CD45+ cells ...
-
bioRxiv - Developmental Biology 2022Quote: ... Stefano Rivella) and CD34+ cells were isolated by positive selection using the human CD34 Microbead kit from Miltenyi Biotec (Catalog No.130-046-702) as per manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... Myelin debris was removed from the cell suspensions using Myelin Removal Beads II kit (Miltenyi Biotec) as described above but with 1 min longer centrifugation steps ...
-
bioRxiv - Developmental Biology 2022Quote: ... were equilibrated with MACS buffer on a QuadroMACS magnetic separator (Miltenyi Biotec) and as soon as the cell suspension finished centrifugating ...
-
bioRxiv - Developmental Biology 2022Quote: ... myelin debris was removed from the cell suspension using Myelin Removal Beads II kit (Miltenyi Biotec) following the manufacturer’s protocol with minor modifications ...
-
bioRxiv - Developmental Biology 2022Quote: ... The single-cell suspension was then sorted for EPCAM/CD326 positive cells using CD326 microbeads (Miltenyi Biotec 130-061-101), according to the manufacturer’s guidelines.
-
bioRxiv - Developmental Biology 2022Quote: ... Each purified hepatocyte pellet was resuspended in 5ml of red blood cell (RBC) lysis solution (Miltenyi Biotec 130-094-183) and incubated at room temperature for 10 mins ...
-
bioRxiv - Developmental Biology 2022Quote: ... and MACS MS columns (Miltenyi biotec 130-042-201). Purified Trophoblasts were seeded in DMEM-HAM’s F12 (Gibco 31331-28)/ 10% FBS (Sigma S0615-500ML ...
-
bioRxiv - Microbiology 2022Quote: ... and anti-PE-conjugated (Miltenyi Biotec, cat#130-048-801) microbeads were added and incubated on ice for 30 min ...
-
bioRxiv - Microbiology 2022Quote: ... 50 μL each of anti-APC-conjugated (Miltenyi Biotec, cat#130–090– 855) and anti-PE-conjugated (Miltenyi Biotec ...
-
Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... PBMCs were stimulated for 3 hours with 10 μg/mL of Gag AK11 peptide and T cells producing IFN-γ in response were enriched using the IFN-γ Secretion Detection and Enrichment Kit (130-054-201; Miltenyi Biotec, Bergisch Gladbach, Germany) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... 3 × 105 cells harvested from the spleen or cervical lymph nodes were incubated for 16 h in the presence of a peptide pool derived from the S protein of SARS-CoV-2 (a mixture of PepTivator SARS-CoV-2 Prot_S, S1, and S+; Miltenyi Biotec, Bergisch Gladbach, Germany). After washing the cells with PBS ...
-
bioRxiv - Neuroscience 2022Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec) were added to the medium ...
-
bioRxiv - Neuroscience 2022Quote: ... 2 μM Purmorphamine (Miltenyi Biotec), 100 ng/ml FGF8 (Peprotech ...
-
bioRxiv - Pathology 2022Quote: ... Hearts were digested for 30 min at 37°C in gentleMACS C Tubes (Miltenyi Biotec) with 800 U/ml Collagenase II and 100 U/ml DNase I (Worthington Biochemical Corporation) ...
-
bioRxiv - Pathology 2022Quote: ... The dead cells were removed using Dead Cell Removal Kit (Miltenyi Biotec). Cell number and viability were analyzed using trypan blue staining (Invitrogen) ...
-
bioRxiv - Neuroscience 2022Quote: ... then washed in PBS and dissociated using the gentleMACS Dissociator (Miltenyi Biotec) in program NTDK1 using the enzyme mix ...
-
bioRxiv - Systems Biology 2022Quote: ... 10 organoids were dissociated with the papain-based dissociation kit (Miltenyi Biotec, 130-092-628) described previously ...
-
bioRxiv - Systems Biology 2022Quote: ... Single cell suspensions were obtained by adapting the protocol described in (Cowan et al., 2020) and using a papain-based dissociation kit (Miltenyi Biotec, 130-092-628). Briefly ...
-
bioRxiv - Systems Biology 2022Quote: ... The dead cell removal was performed using Dead Cell Removal Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...