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Citations for Miltenyi Biotec :
4451 - 4500 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and dissociated with MACS MultiTissue Dissociation Kit 3 (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Data was acquired using a MACSQuant Analyzer 10 (Miltenyi Biotec, Auburn, CA) and analyzed using FlowJo (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor cells were collected by Magnetic-activated cell sorting (MACS) using CD19 beads (Miltenyi Biotec). Samples were split into aliquots for proteomics analysis (1×107 cells) ...
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bioRxiv - Cell Biology 2022Quote: ... and positively selected using AutoMACS ProSeparator (Miltenyi Biotec). CD14+ cells were then stained with biotinylated anti-human CD73 antibody and streptavidin microbeads ...
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bioRxiv - Cell Biology 2022Quote: ... autologous T cells were isolated from patient NSCLC lesions or from healthy donor PBMCs using the Pan T-cell Isolation Kit (Miltenyi Biotec). Autologous T cells were co-cultured with either ...
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bioRxiv - Cell Biology 2022Quote: ... Fresh tumor tissue from NSCLC patients were enzymatically digested and single-cell suspensions were stained with CD14 microbeads (Miltenyi Biotec) and positively selected using AutoMACS ProSeparator (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2022Quote: B cells were purified by negative selection from single-cell suspensions from spleen using magnetic separation B cell isolation kit (Miltenyi Biotec 130-090-862). Purified cells were cultured for 4 days in RPMI (Corning ...
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bioRxiv - Cancer Biology 2022Quote: ... Human CD34-PE (Miltenyi Biotec);and Human CD33-APC-Cy7 (Abcam).
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bioRxiv - Cancer Biology 2022Quote: ... and processed using the tumor dissociation kit (130-096-730, Miltenyi Biotec) as recommended by supplier ...
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bioRxiv - Neuroscience 2022Quote: ... human BDNF (50 µL, 100 ng/mL, MACS Miltenyi Biotec, 130-096-286), LM22A4 (25 µL ...
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bioRxiv - Neuroscience 2022Quote: ... Motoneurons were isolated from the spinal cord of Smn2B/+ and Smn2B/- embryos using 5.2% iodixanol density gradient centrifugation combined with p75-based magnetic cell isolation (Miltenyi Biotec) as we previously described (Soulard et al. ...
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bioRxiv - Microbiology 2022Quote: ... stained samples were diluted 10-fold in 1x PBS and cell concentrations as well as green fluorescence intensities were recorded on a MACSQuant Analyzer 10 (Miltenyi Biotec, Germany).
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bioRxiv - Microbiology 2022Quote: ... subjected to 30 µm-filtration (Miltenyi Biotec, Bergisch Gladbach, Germany) and added to anaerobic basal medium (described in (19) ...
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bioRxiv - Microbiology 2022Quote: ... including a 30 µm-filtration step (Miltenyi Biotec, Bergisch Gladbach, Germnay). For in vitro samples ...
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bioRxiv - Molecular Biology 2022Quote: ... CD31- PE/Cy7 (Biologend Inc., Cat# 303117) and TM4SF1-APC (Miltenyi Biotec Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... MACS NeuroBrew-21 with retinoic (10 mL, MACS Miltenyi Biotec, 130- 093-566), PD0332991 (100 µL ...
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bioRxiv - Neuroscience 2022Quote: ... 8 well chamber slide dishes (Miltenyi Biotec) were coated over night with 1 mg/ml poly-L-lysine in borate buffer (boric acid ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed and resuspended in MACS buffer plus 1:5 anti-mouse igG microbeads (Miltenyi Biotec) and rotated at 4°C for a further 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... hiPSCs were grown until 100% confluent on 0.4μg/cm2 LN521 basement matrix (BioLamina) in StemMACS iPS-brew XF with iPSbrew supplement (1X) (Miltenyi Biotec). Cells were then passaged 1:3 as clumps using 15 min 37°C incubation with 1mg/ml collagenase IV (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then resuspended in 1ml MACS buffer and applied to a MS magnetic column mounted to an OctoMACS magnet (Miltenyi Biotec). Cells were washed 3x with 500μl MACS buffer then the positive fraction eluted in 1ml MACS buffer.
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bioRxiv - Neuroscience 2022Quote: hiPSCs/ESCs were maintained on 0.4μg/cm2 LN521 basement matrix (BioLamina) in StemMACS iPS-brew XF with iPSbrew supplement (1X) (Miltenyi Biotec) plus Penicillin/streptomycin (1x ...
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bioRxiv - Neuroscience 2022Quote: ... Microglia and astrocytes were respectively isolated using Anti-CD11b and Anti-ACSA-2 MicroBeads (Miltenyi Biotec). Cells were counted and resuspended in 10-15 µL and 20-30 µL of buffer (0.05% BSA in DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... Up to 1.25 grams of cortical tissue were placed in each gentleMACS™ C Tube (Miltenyi Biotec, # 130-093-237) which contained 5 mL of 0.3 mg/mL papain (Worthington ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 80 μL of 0.5% BSA and 20 μL of Anti-Biotin MicroBeads (Miltenyi Biotec, # 130-115-389) and incubated for 10 minutes at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Debris removal solution was made by adding 1800 μL of Debris Removal Concentrate (Miltenyi Biotec, # 130-109-398) to 6200 μL of cold DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... the mixture was flowed through 0.5% BSA primed LS columns (Miltenyi Biotec, #130-042-401). The negative fraction containing the majority of neurons was collected and centrifuged at 300×g for 5 minutes at 4 °C and resuspended in neuron media ...
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bioRxiv - Neuroscience 2022Quote: ... Red Blood Cell Remover Solution was made by mixing 125 μL of Red Blood Cell Lysis Solution 10× (Miltenyi Biotec, # 130-094-183) with 1125 μL of H2O ...
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bioRxiv - Neuroscience 2022Quote: ... The gentleMACS™ C Tubes were placed on the gentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec, # 130-096-427) with heating cuffs attached and underwent the gentleMACS Program 37C_ABDK_01 protocol ...
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bioRxiv - Neuroscience 2022Quote: ... neuron media consists of MACS Neuro Media (Miltenyi Biotec, # 130-093-570), 2 mM L-alanine-L-glutamine dipeptide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... the contents of the gentleMACS™ C Tube were strained through a 70 μm cell strainer (Miltenyi Biotec, # 130-110-916) placed on top of a 15 mL conical centrifuge tube ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 80 μL of 0.5% BSA and 20 μL of Non-Neuronal Cells Biotin-Antibody Cocktail (Miltenyi Biotec, # 130-115-389) and incubated for 5 minutes at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 1.25 mL of Red Blood Cell Remover Solution and incubated for 10 minutes at 4 °C before the addition of 12 mL of 0.5 % bovine serum albumin (BSA, Miltenyi Biotec, # 130-091-376) diluted in DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... per sample into the magnet separator (Miltenyi Biotec) and rinse with 2 ml FACS buffer ...
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bioRxiv - Neuroscience 2022Quote: ... the suspensions were washed twice and filtered through the myelin removal column (Miltenyi Biotec) in 15-ml conical tubes on ice ...
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bioRxiv - Physiology 2022Quote: ... for 20 minutes at 4°C before Auto MACS cell separation (Miltenyi Biotec, Cologne, Germany). Cells were then sorted with a BD FACSAria Fusion (BD Biosciences ...
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bioRxiv - Physiology 2022Quote: ... samples were resuspended and incubated with 80 μL anti-PE microbeads per 107 cells (Miltenyi Biotec, Cologne, Germany) for 20 minutes at 4°C before Auto MACS cell separation (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2022Quote: ... We used our previous established isolation protocol37 using gentleMACS™ with the Neural Tissue Dissociation Kit (Papain) (Miltenyi Biotec) and a final concentration of 45 mM actinomycin D (Act-D ...
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bioRxiv - Neuroscience 2022Quote: Day 37 neurons were harvested using the Miltenyi MACS human mitochondria isolation kit (Miltenyi Biotec 130-094-532) with additional proteases added to the lysis buffer aprotinin (10 μg/ml) ...
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bioRxiv - Neuroscience 2022Quote: ... CD11b+ cells were collected using magnetic field columns (Miltenyi Biotec). Cell suspensions (35 μl ...
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bioRxiv - Neuroscience 2022Quote: ... The Neural Tissue Dissociation Kit - P (NTDK, #130-092-628, Miltenyi Biotec) based on the enzymatic dissociation with papain was used to homogenize the tissue ...
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bioRxiv - Microbiology 2022Quote: ... T cells were enriched from spleen homogenate by magnetic-activated cell sorting (MACS) using Miltenyi Pan T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... myelin was removed using debris removal solution (Miltenyi Biotec, 130-109-398) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... Streptavidin pull-down was performed with the μMACS Streptavidin Kit (Miltenyi Biotec), washing the columns three times with 55°C pull-down wash buffer (100 mM Tris HCl ...
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bioRxiv - Neuroscience 2022Quote: ... cells were magnetically labeled with CD11b microbeads (#130-093-634; Miltenyi Biotec) diluted in PBS supplemented with 0.5% BSA for 15 minutes in the dark in the refrigerator (2-8 °C) ...
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bioRxiv - Neuroscience 2022Quote: Microglia cells from adult young or old mice were isolated and cultured using immunomagnetic separation (Miltenyi Biotec, Germany). Mice were perfused via the left ventricle with 60 mL of cold saline and collected in HBSS buffer without calcium/magnesium (#14175-05 ...
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bioRxiv - Neuroscience 2022Quote: ... Brain cells were incubated with Myelin Removal Beads II (#130-096-733, Miltenyi Biotec) and then passed through LS Columns (#130-042-401 ...
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bioRxiv - Neuroscience 2022Quote: ... and to the MACS® MultiStand (#130-042-303, Miltenyi Biotec). Unspecific binding of antibodies was blocked by previous incubation for 10 min with anti CD16/CD32 (Fc block ...
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bioRxiv - Neuroscience 2022Quote: ... held onto the OctoMACS Separator (#130-091-051, Miltenyi Biotec) and to the MACS® MultiStand (#130-042-303 ...
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bioRxiv - Neuroscience 2022Quote: ... The brain tissue was enzymatically dissociated using the Neural Tissue Dissociation Kit (P) (#130-092-628, Miltenyi Biotec). The gentleMACS™ Dissociator (#130-096-427 ...
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bioRxiv - Neuroscience 2022Quote: Membrane potential measurements were made by impaling pericytes on microvessels isolated from Cspg4-DsRed mice using a papain-based Neural Tissue Dissociation kit (Miltenyi Biotec), as described previously21,25 ...