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Citations for Miltenyi Biotec :
351 - 400 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Isolation of untouched CD8+ T cells from total PBMCs was performed using a two-step human CD8+ T cell isolation kit (Miltenyi Biotec). All steps were performed following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: Single-cell suspensions were blocked with FcR Blocking Reagent (Miltenyi Biotec) and stained with cocktails of fluorochrome-conjugated antibodies ...
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bioRxiv - Immunology 2024Quote: ... B cells and T cells (Miltenyi Biotec) were used ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were negatively selected from the minced auricular lymph nodes using the CD4+ T cell isolation kit (Miltenyi Biotec) and cultured in RPMI 1640 media supplemented with 25 mM HEPES ...
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T-cell development and activation in humanized mice lacking mouse major histocompatibility complexesbioRxiv - Immunology 2024Quote: ... CD34+ cells were positively selected through two consecutive runs using immune magnetic beads kits (Miltenyi Biotec, Bergisch-gladbach, Germany) and cryopreserved ...
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bioRxiv - Immunology 2024Quote: ... CD14+ human monocytes were isolated from fresh peripheral blood mononuclear cells (PBMCs) using CD14 MACS MicroBeads (Miltenyi Biotec, Cat. No. 130-050-201) and cultured in MoDC differentiation medium at a density of 106 cells/ml for 6 days ...
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bioRxiv - Immunology 2024Quote: ... Percentage of monocytes and immature MoDC cells were analyzed in a MACSQuant® flow cytometer (Miltenyi Biotec). Flow cytometry data were analyzed using FlowJo [version 10.0.7 (TreeStar)].
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bioRxiv - Immunology 2024Quote: ... Percentage of transfected cells was analyzed in a MACSQuant® flow cytometer (Miltenyi Biotec). CD14+ monocytes and inmature MoDC were frozen in fetal bovine serum (FBS ...
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bioRxiv - Genomics 2024Quote: ... CD73 negative neuronal cells were enriched by autoMACS Pro Separator (Miltenyi Biotec) DEPLETES mode ...
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bioRxiv - Immunology 2024Quote: ... Lung lobes were excised and dissociated in C tubes (Miltenyi Biotec) in HEPES buffer containing Liberase Blendzyme 3 (70 mg/ml ...
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bioRxiv - Immunology 2024Quote: ... and depleted of mature T cells using a CD3ε depletion kit (Miltenyi Biotec, #130-094-973). 1-2×106 bone marrow cells were then injected retro-orbitally to each host mouse under isoflurane anesthesia ...
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bioRxiv - Immunology 2024Quote: ... containing 1mL PBS+0.05% Tween-80 (PBS-T) using a GentleMACS tissue dissociator (Miltenyi Biotec). Organ homogenates were then diluted in PBS-T and aliquots were plated onto 7H10 agar media to quantify Mtb burden ...
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bioRxiv - Immunology 2024Quote: Mouse lung lobes and spleens were homogenized in M tubes (Miltenyi Biotec) containing 1mL PBS+0.05% Tween-80 (PBS-T ...
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bioRxiv - Immunology 2024Quote: ... using a GentleMACS dissociator (Miltenyi Biotec). Lung homogenates were incubated for 30 minutes at 37°C and further processed with the GentleMACS dissociator ...
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bioRxiv - Immunology 2024Quote: Frozen tissue pieces were quickly thawed in a water bath before transferring into a GentleMACS dissociation tube (Miltenyi Biotec) containing Advanced DMEM/F12 + 1:100 Glutamax (Gibco ...
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bioRxiv - Immunology 2024Quote: ... NKG2C+ NK cells were then selectively isolated as aNK cells using anti-PE microbeads (Miltenyi Biotec), while the flow-through ...
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bioRxiv - Immunology 2024Quote: ... we initially isolated bulk NK cells following the manufacturer’s guidelines (Miltenyi Biotec). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulation was induced with anti-CD3ε and anti-CD28 beads (T Cell Activation/Expansion Kit mouse, Cat.#130-093-627, Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Lungs from infected mice were dissociated using scissors or a GentleMACS dissociator (Miltenyi Biotec) and cells were isolated and analyzed as previously described 190 ...
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bioRxiv - Immunology 2024Quote: ... containing Fc receptor blocker (Miltenyi Biotec) and incubated for 10 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells (used for in vivo experiments) were supplied with 0.5ng/ml IL15 (Miltenyi Biotec) and 10ng/µl IL7 (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... 3×105 T cells were plated per condition in 150 μl of TexMACS medium (Miltenyi Biotec) without cytokines ...
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bioRxiv - Immunology 2024Quote: ... and 10ng/µl IL7 (Miltenyi Biotec) (17) ...
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bioRxiv - Immunology 2024Quote: Cytokines quantification has been performed using the MACSPlex Cytokine 12 Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and L1CAM (Miltenyi Biotec) was detected by fluorophore-conjugated monoclonal antibodies on a Fortessa X-20 (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Cells were activated with TransAct (1:150, Miltenyi Biotec) or PBS (negative activation control ...
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bioRxiv - Immunology 2024Quote: ... suspended cells (mostly T cells) were separated and cultured with TexMACS (Miltenyi Biotec) supplemented with IL-7 and IL-15 (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... supplemented with IL-7 and IL-15 (Miltenyi Biotec), while the adherent cells (monocytes/macrophages ...
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bioRxiv - Immunology 2024Quote: ... CD8+ L1CAM-CAR T cells (used for in vitro experiments) were generated and expanded as previously described (16) and supplied with 0.5ng/ml IL15 (Miltenyi Biotec) and 50U/ml IL2 (Novartis) ...
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bioRxiv - Microbiology 2024Quote: Tissue was mechanically homogenized in QIAzol lysis buffer using a gentleMACS™ dissociator (Miltenyi Biotec, Germany) and total RNA was isolated using miRNeasy Mini Kits (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... Primary CD4+ T-cells and monocytes were purified from PBMCs by positive selection using CD4 (Miltenyi Biotec; 130-045-101) and CD14 (Miltenyi Biotec ...
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bioRxiv - Microbiology 2024Quote: ... and CD14 (Miltenyi Biotec; 130-097-052) magnetic microbeads ...
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bioRxiv - Immunology 2024Quote: ... CD8- thymocytes were obtained by negative selection using anti-CD8 (Ly-2) MicroBeads (Miltenyi Biotec). The cells were then stained with fluorescently labeled antibodies to CD4 ...
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bioRxiv - Immunology 2024Quote: ... CD14+/CD16+ monocytes were isolated by negative selection magnetic bead column purification (Pan Monocyte Isolation Kit, Miltenyi Biotec). For chromatin accessibility (ATAC-seq) ...
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bioRxiv - Immunology 2024Quote: ... F4/80-FITC (Miltenyi Biotec), and Ly6C-Pacific blue (MACS) ...
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bioRxiv - Immunology 2024Quote: ... IFN-α (LT27:295, Miltenyi Biotec). PI (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and CD4 and CD8 cells were purified using magnetic beads (Miltenyi Biotec) and combined at a 1:1 ratio before freezing ...
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bioRxiv - Immunology 2024Quote: ... wells were stained in cold MACS buffer (Miltenyi Biotec, Cat. No.130-091-221) at 4°C for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... or TexMACS Cell Culture Medium (Miltenyi Biotec) supplemented with 10ng IL-7 (Biotechne) ...
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bioRxiv - Immunology 2024Quote: ... or 10μL/mL T-cell TransAct αCD3/αCD28 (Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2024Quote: ... falciparum infected erythrocytes using a MACS cell separator with LD columns (Miltenyi Biotec #130-042-901), and diluted to 10 × 106 infected erythrocytes per mL in PBS ...
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bioRxiv - Immunology 2024Quote: ... using a CD4+ T-cell isolation kit from Miltenyi Biotec Inc ...
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bioRxiv - Immunology 2024Quote: ... Myelin debris was removed by resuspending the cell pellet with a debris removal solution (Miltenyi Biotec), overlaying with PBS ...
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bioRxiv - Immunology 2024Quote: ... untouched Pan T cells were enriched by negative selection using T cell isolation kit II (Miltenyi biotec). All donor tissue information is included in Table S1.
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bioRxiv - Immunology 2024Quote: ... +/− 100 nM rapamycin (Miltenyi Biotec) and human Treg expansion kit anti-CD3/CD28 beads (Miltenyi Biotec/Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Cells were collected for TSDR demethylation analysis and stored at −80 as cell pellets or expanded with 500-1000 U/mL human rIL2 (Miltenyi Biotec/Chiron) +/− 100 nM rapamycin (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... and human Treg expansion kit anti-CD3/CD28 beads (Miltenyi Biotec/Invitrogen), using x-vivo 15 cell medium (Lonza ...
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bioRxiv - Immunology 2024Quote: ... then followed by another incubation with anti-APC Microbeads (Miltenyi Biotec, #130-090-855). Negative enrichment to remove red blood cells ...
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bioRxiv - Microbiology 2024Quote: ... and anti-CD25-APC (Miltenyi Biotec, Cat#1130-115-535) antibodies according to the manufacturer’s instructions ...