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Citations for Miltenyi Biotec :
251 - 300 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The cells were isolated from the colon lamina propria using the “Lamina Propria Dissociation Kit” from Miltenyi Biotec according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... ∼30h after transfection cells were submitted to MACS column selection using MS columns (130-042-201, Miltenyi Biotec) and LNGFR MicroBeads (130-091-330 ...
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bioRxiv - Molecular Biology 2024Quote: ... Each sample was dissociated for 15 min at 37 °C on the GentleMACS Octo Dissociator with Heaters (Miltenyi) using Human Tumor Dissociation Kit (Miltenyi Biotec). Following tissue dissociation ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells were isolated using Human Naïve CD4+ T Cell Isolation Kit II (Miltenyi Biotec). CD14+ monocytes were isolated using Human CD14 Microbeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... CD14+ monocytes were isolated using Human CD14 Microbeads (Miltenyi Biotec). Cell purity was verified by flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from PBMC using Human CD4+ T Cell Isolation Kits (Miltenyi Biotec, Inc., Bergisch Gladbach, Germany). Naïve CD4+ T cells were isolated using Human Naïve CD4+ T Cell Isolation Kit II (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2024Quote: ... cell isolation was performed using a magnetic cell sorting (MACS) method following the manufacturer’s instructions (Miltenyi Biotec, The Netherlands). Isolation of specific cell types was performed separately in male and female cultures An anti-CD11b microbeads antibody (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2024Quote: Separator attached to the MACS MultiStand (Miltenyi Biotec). Afterwards ...
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bioRxiv - Immunology 2024Quote: ... activated with a nonhuman primate T-Cell Activation/Expansion Kit (Miltenyi Biotec), and cultured in RPMI-1640 medium supplemented with 1×Glutamax (Life Technologies) ...
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bioRxiv - Immunology 2024Quote: ... IMhu-M cells were cultivated in IL-4/GM-CSF MoDC differentiation medium (Miltenyi Biotec, Cat. No. 130-094-812), when indicated ...
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bioRxiv - Developmental Biology 2024Quote: ... 20 ng/mL human recombinant FGF-2 (all from Miltenyi Biotec)) and growth factor-reduced Matrigel (Corning ...
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bioRxiv - Genomics 2024Quote: ... we processed a fraction of the dissociated tumor for immune cell depletion using CD11b MicroBeads (Miltenyi Biotec #130-097-142) following manufacturer’s protocol on LS Columns ...
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bioRxiv - Immunology 2024Quote: CAR+ T-cells were purified using a biotin-conjugated AffiniPure Goat Anti-Mouse IgG F(ab’)₂ fragment specific antibody and anti-biotin microbeads (Miltenyi Biotec). In the case of dual knock-in TET2-TRAC CAR T-cells ...
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bioRxiv - Immunology 2024Quote: ... or anti-PE MultiSort microbeads as per the manufacturer’s instructions (Miltenyi Biotec). Purity was assessed by flow staining against knock-in markers tEGFR and tNGFR ...
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bioRxiv - Immunology 2024Quote: T-cells were isolated from healthy donor PBMCs using the Pan T-cell Isolation Kit following manufacturer’s instructions (Miltenyi Biotec). Isolated T-cells were activated using anti-CD3/CD28 antibody-coated Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells were enriched via positive selection (Miltenyi Biotec), resuspended in TRIzol™ (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... and 4 ng/mL of recombinant basic fibroblast growth factor (Miltenyi Biotec, Germany). Cells were cultured for 2 passages at a seeding density of 5×10 cells/cm.
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bioRxiv - Cancer Biology 2024Quote: ... Dead cells were removed using the dead cell removal kit (Miltenyi Biotec, 130-090-101) per the manufacturer protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... using the human tumor dissociation kit (Miltenyi Biotec, 130-095-929). Dead cells were removed using the dead cell removal kit (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were harvested from mice on Day 3 and separately dissociated on a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Bioengineering 2024Quote: ... Canada) for 48 hours or T-cell TransAct (10 uL for each mL of culture, Cat # 130-111-160, Miltenyi Biotec, Bergisch Gladbach, Germany) for 72 hours respectively.
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bioRxiv - Immunology 2024Quote: ... Human monocytes were negatively selected from PBMCs using the monocytes Isolation Kit (Miltenyi Biotec). Monocytes were then plated 0.15×106/well onto 96-well plate (for ELISA ...
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bioRxiv - Bioengineering 2024Quote: ... reactions were labeled with 12μl anti-c-myc-FITC (Miltenyi Biotec), 5μl SAPE (streptavidin-R-phycoerythrin ...
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bioRxiv - Bioengineering 2024Quote: ... Thermo) were washed 3 times with PBS followed by incubation with biotin anti-mouse CD63 antibody (Clone REA563; Miltenyi Biotec) or biotin anti-mouse fibronectin (FN ...
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bioRxiv - Neuroscience 2024Quote: ... with the addition of SHH-C24II (200 ng/ml, Miltenyi Biotec, 130-095) between days 7 and 10 of the differentiation protocol ...
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bioRxiv - Neuroscience 2024Quote: Cleared and immunolabeled intact tissues were imaged using an UltraMicroscope Blaze (Miltenyi Biotec). All images were acquired using right-side illumination ...
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bioRxiv - Neuroscience 2024Quote: The mouse MACSPlex exosome kit (Miltenyi Biotec, 130-122-211) was used to detect EV specific markers using BD LSRFortessa (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... live primary T cell populations with anti-CD4-PE (Miltenyi Biotec, 130-113-225), anti-CXCR4-APC (Miltenyi Biotec ...
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bioRxiv - Microbiology 2024Quote: ... anti-CXCR4-APC (Miltenyi Biotec, Cat#130-120-708), and anti-CD25-APC (Miltenyi Biotec ...
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bioRxiv - Microbiology 2024Quote: ... Splenocytes were stimulated with SARS-CoV-2 S peptide pools (2 μg/ml, Miltenyi Biotec) for 24 h at 37°C ...
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bioRxiv - Microbiology 2024Quote: Splenocytes were incubated with SARS-CoV-2 S peptide pools (1μg/ml, Miltenyi Biotec) for 6 h in the presence of GolgiPlug (BD Bioscience) ...
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bioRxiv - Microbiology 2024Quote: ... Samples were passed through μ Columns (MACS purification system, Miltenyi Biotec). The μ Columns were then washed 4 times with 200 μL of WASH buffer1 and 1 time with 200 μL of WASH buffer 2 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were divided into equal parts (250 μL each) and incubated with 50 μL of magnetic microparticles coated with monoclonal HA or MYC antibodies (MACS purification system, Miltenyi Biotec) at 4°C for 1 hour under rotation (10 rpm) ...
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bioRxiv - Microbiology 2024Quote: Primary CD4+ T cells were purified from PBMCs of a healthy donor by negative selection (Miltenyi Biotec, USA). Purified CD4+ T cells were stimulated with 1 µg/mL soluble anti-CD3 (clone OKT3 ...
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bioRxiv - Cancer Biology 2024Quote: ... EpCAM (FITC, Miltenyi Biotec 130-110-998) and PDPN (APC ...
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bioRxiv - Cancer Biology 2024Quote: ... Tissue homogenization was facilitated by use of the gentleMACS dissociator (Miltenyi Biotec, 130-093-235). Samples were then centrifuged at 4,000 x g for 5 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The purity of the NK cell population was validated by flow cytometry using PE-Vio 770 anti-human CD56 (1:50; REA196 clone; Miltenyi Biotec) and FITC anti-human CD3 (1:50 ...
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bioRxiv - Cancer Biology 2024Quote: ... The Human Tumor Dissociation Kit (Miltenyi Biotec 130095929) was used per the manufacturer’s protocol to generate a single-cell suspension from the tumor sample ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary NK cells were extracted from PBMCs by negative immunomagnetic isolation using a NK cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by loading onto a LS Separation column (Miltenyi Biotec). The cell suspension was then placed in the magnetic field of a MACS Separator to separate labeled from non-labeled cells ...
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bioRxiv - Cancer Biology 2024Quote: ... and FITC anti-human CD3 (1:50; REA613 clone; Miltenyi Biotec) antibodies.
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bioRxiv - Cancer Biology 2024Quote: ... T-ALL cells were isolated from the spleen and bone marrow of mice by negative selection with mouse CD45 microbeads (Miltenyi Biotec #130-052-301).
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bioRxiv - Cancer Biology 2024Quote: ... BM from CD45.1+ mice for hemogenic support were subjected to CD3 depletion (Miltenyi Biotec #130-094-973) and cells were harvested and washed with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Bone marrow (BM) from CD45.2 mice was subjected to Lineage depletion (Miltenyi Biotec #130-110-470). CD45.2 BM cells were plated at 1×106 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (Thermo Fisher #10639011 ...
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bioRxiv - Cancer Biology 2024Quote: Flow cytometry was performed on MACSQuant Analyzer 10 (Miltenyi Biotec, Bergisch Gladbach, Germany), and analysis of data was performed using FlowJo 10 (FlowJo LLC ...
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bioRxiv - Cancer Biology 2024Quote: Tissues samples were homogenized and lysed using gentleMACS Dissociators (Catalog No. 130-093-235, Miltenyi Biotec) with RIPA buffer ...
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bioRxiv - Cancer Biology 2024Quote: Protein extraction and digestion procedures were as follows: Tissues were homogenized and lysed using gentleMACS Dissociators (Catalog No. 130-093-235, Miltenyi Biotec, Nordrhein-Westfalen, Germany) with RIPA buffer (Catalog No ...
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bioRxiv - Microbiology 2024Quote: ... and CD14 (Miltenyi Biotec; 130-097-052) magnetic microbeads ...
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bioRxiv - Microbiology 2024Quote: Tissue was mechanically homogenized in QIAzol lysis buffer using a gentleMACS™ dissociator (Miltenyi Biotec, Germany) and total RNA was isolated using miRNeasy Mini Kits (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... and subsequently CD4 microbeads for T helper cells (all Miltenyi Biotec, Bergisch Gladbach, Germany). The homogeneity of the cell preparations was controlled by flow cytometry.