Labshake search
Citations for Miltenyi Biotec :
601 - 650 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... in gentleMACS C Tubes (Miltenyi Biotec) for 42 min at 37 °C using the gentleMACS Octo Dissociator ...
-
bioRxiv - Cancer Biology 2024Quote: ... The tumors were dissociated into single-cell suspension using the tumor dissociation kit (Miltenyi Biotec, 130-095-929) following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... and tumor necrosis factor (TNF) were determined by MACSPlex Cytokine 12 Kit (Miltenyi Biotec). Supernatants of tumor cell lines were also used for the measurement of FGL-1 with the Human FGL1/Fibrinogen Like Protein 1 ELISA Kit (Assay Genie ...
-
bioRxiv - Cancer Biology 2024Quote: ... 3.5 µL 1 mg/mL Hoechst 33342 dye and 399 µL of permeabilization buffer Inside Perm (#130-090-477, Inside stain Kit, MACS Miltenyi Biotec).
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked for 30 min and stained with 2μl of human EpCAM (FITC conjugated, Miltenyi Biotec, 130-080-301) and 1:75 of mouse MHC class I (APC conjugated ...
-
bioRxiv - Cancer Biology 2024Quote: Cleared samples were imaged with a light-sheet microscope (Ultramicroscope II, Miltenyi Biotec, Heidelberg, Germany) using a 4x objective (MI Plan objective lens 4x ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were pelleted and resuspended in TMRE buffer and subjected to flow cytometry on a MACSQuant Analyzer (Miltenyi Biotec). For mitochondrial superoxide measurements ...
-
bioRxiv - Cancer Biology 2024Quote: ... T cells were isolated using the Pan T cell isolation kit (Miltenyi Biotec, Germany).13
-
bioRxiv - Cancer Biology 2024Quote: ... single cell suspension was thawed into TexMACS media (Miltenyi Biotec, 130-097-196) and incubated at 37°C for 1 hour to allow cell recovery ...
-
bioRxiv - Cancer Biology 2024Quote: ... frozen single cell suspensions from MDOTS processing (see above) were thawed and CD8+ TILs were isolated using Cd8a (LY-2) Microbeads (Miltenyi Biotec, 130-117-044). T cells were cultured in RPMI (Corning ...
-
bioRxiv - Cancer Biology 2024Quote: CD34+ HSPC were enriched from cord blood mononuclear cells (MNC) by magnetic selection using anti-CD34 conjugated magnetic beads according to the manufacturer’s instructions (Miltenyi Biotec, 130-100-453). To obtain a sufficient cell number for ImageStream and Western blot analysis ...
-
bioRxiv - Cell Biology 2024Quote: ... Autologous CD4+ T cells were isolated from PBMCs with a negative CD4+ T cell isolation kit (130-096-533, Miltenyi Biotec).
-
bioRxiv - Cell Biology 2024Quote: ... The cells were stained with APC-efluo780 conjugated anti-c-kit antibody and c-kit+ cells were enriched using anti-APC paramagnetic microbeads (Miltenyi Biotec). Cells were stained with fluorophore-conjugated antibodies against lineage markers (CD2 ...
-
bioRxiv - Biophysics 2024Quote: ... mouse and rat according to manufacturer’s instructions (MACS Miltenyi Biotec, but without the gentleMACS Dissociator) ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were cultured regularly on tissue culture plastic (TCP) coated with vitronectin (0.5 µg/cm2) in StemMACS iPS-Brew (Miltenyi Biotec, Bergish Gladbach, Germany) and passaged regularly using intermittent Ethylenediaminetetraacetic acid (EDTA ...
-
bioRxiv - Cell Biology 2024Quote: ... in StemMACS™ iPS-Brew XF medium (Miltenyi Biotec, 130-104-368) supplemented with Y-27632 (10 μM) ...
-
bioRxiv - Cell Biology 2024Quote: ... the samples were transferred to a plastic tube containing a knife and placed on the gentleMACS Dissociator (Miltenyi Biotec) to be further dissociated and then incubated with enzymes for 30 min at 37°C under continuous rotation ...
-
bioRxiv - Cell Biology 2024Quote: The tissue pieces remaining after enzymatic treatment and filtration of the fresh sample were processed using a human tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions to obtain a single-cell suspension ...
-
bioRxiv - Cell Biology 2024Quote: PBMCs were negatively isolated by a PBMC isolation kit (130-115-169, Miltenyi Biotec Inc., CA) from human buffy coats provided by the Hong Kong Red Cross ...
-
bioRxiv - Cell Biology 2024Quote: ... The wound tissues underwent enzymatic epidermal-dermal separation using the Epidermis Dissociation Kit (Epidermis Dissociation Kit, mouse; Miltenyi Biotec) followed by dissociation of the epidermis and dermis parts ...
-
bioRxiv - Cancer Biology 2024Quote: ... Fluorescent intensity of 10 000 cells was detected by flow cytometry on a FACS instrument (MACSQuant®VYB, Miltenyi Biotec, San Diego, CA, USA). Data were analyzed using FlowJo software (FlowJo LLC ...
-
bioRxiv - Cancer Biology 2024Quote: ... Tumors were detached from the mice and approximately 150 mg of tumor tissue was enzymatically and mechanically digested using the Mouse Tumor Dissociation Kit (Miltenyi Biotec) to obtain a single cell suspension ...
-
bioRxiv - Cancer Biology 2024Quote: ... REA Control-VioBlue (REA293, Miltenyi Biotec) and Mouse IgG2bk-PB450 (MPC-11) ...
-
bioRxiv - Cancer Biology 2024Quote: ... CD14-PerCP (TUK4, Miltenyi Biotec) CD86-PE-Cy7 (2331 ...
-
bioRxiv - Cancer Biology 2024Quote: ... following the manufacturers’ protocols (Miltenyi Biotec). Immature DC (iDC ...
-
bioRxiv - Cancer Biology 2024Quote: ... HLA-ABC-VioBlue (REA230, Miltenyi Biotec), HLA-DR/DP/DQ-FITC (Tu39 ...
-
bioRxiv - Cancer Biology 2024Quote: Human: CD11c APC-Vio770 (MJ4-27G12, Miltenyi Biotec), CD14-PerCP (TUK4 ...
-
bioRxiv - Bioengineering 2024Quote: ... and mouse (Miltenyi Biotec, cat.: 130-092-575) for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail ...
-
bioRxiv - Bioengineering 2024Quote: ... and CliniMACS Tubing Set LS (Miltenyi Biotec, Bergisch Gladback, Germany). CD34+ and CD34- cell fractions were stained with CD34-PE (Clone ...
-
bioRxiv - Bioengineering 2024Quote: ... CD271/NGFR (Miltenyi Biotec) MHC-I and MHC-II (BD Biosciences ...
-
bioRxiv - Cancer Biology 2024Quote: ... following manufacturers guidelines (Miltenyi Biotec). Purity of the sorting was confirmed by flow cytometry using a BD Lyric flow cytometer (BD Biosciences).
-
bioRxiv - Bioengineering 2024Quote: ... at a ratio of 1:2 in NK MACS media (Miltenyi Biotec, Gaithersburg, MD) supplemented with 5% human AB serum (Millipore Sigma ...
-
bioRxiv - Bioengineering 2024Quote: ... unedited and nucleofected NK cells were cultured in complete NK MACS medium (Miltenyi Biotec, Gaithersburg, MD) with 100 IU/mL IL-2 and 2 ng/mL IL-15 ...
-
bioRxiv - Bioengineering 2024Quote: ... and 1% MACS supplement (Miltenyi Biotec, Gaithersburg, MD). Cells were cultured until day 4 ...
-
bioRxiv - Bioengineering 2024Quote: ... Post-nucleofection recovery medium consisting of Complete NK MACS medium (Miltenyi Biotec, Gaithersburg, MD) with 100 IU/mL IL-2 and 2 ng/mL IL-15 +/− 0.6uM M3814 (Selleck Chemicals ...
-
bioRxiv - Bioengineering 2024Quote: ... isolated mononuclear cells were positively selected for CD34 using the CD34+ Microbead kit Ultrapure (Miltenyi Biotec, San Diego, CA, USA, cat.: 130-100-453). Cells were cultured at 1.5×105–2.5×105 cells/mL in CellGenix® GMP Stem Cell Growth Medium (SCGM ...
-
bioRxiv - Bioengineering 2024Quote: ... chambers were obtained from the Stanford Blood Center and primary human B cells were isolated by negative selection using the human B Cell Isolation Kit II (Miltenyi Biotec, cat: 130-091-151). Cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM ...
-
bioRxiv - Bioengineering 2024Quote: ... or BV650-conjugated anti-CD56 (clone 5.1H11, BioLegend; clone REA196, Miltenyi Biotec; or clone HCD56, BioLegend), FITC- conjugated anti-CD3 (clone OKT3 ...
-
bioRxiv - Bioengineering 2024Quote: ... All samples were blocked for non-specific binding with FcR Blocking Reagent for human (Miltenyi Biotec, cat.: 130-059-901) and mouse (Miltenyi Biotec ...
-
bioRxiv - Bioengineering 2024Quote: ... human kit (Miltenyi Biotec, cat.: 130-050-301). Knock-in and INDEL frequencies were determined by ddPCR and ICE analysis as described above.
-
bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 20 min at 4°C with an anti-mouse antibody conjugated to magnetic microbeads (Miltenyi Biotec). Finally ...
-
bioRxiv - Bioengineering 2024Quote: Splenocytes were plated in 96-well U-bottom plates at 106 cells/well in RPMI and stimulated with 1 μg/mL of PepTivator Ovalbumin (Miltenyi Biotec). After 3 hours ...
-
bioRxiv - Cancer Biology 2024Quote: ... PBMCs were activated with T cell TransAct polymeric nanomatrix (Miltenyi Biotec) according to manufacturer’s specifications in the presence of 100 IU/mL recombinant human (rh ...
-
bioRxiv - Bioengineering 2024Quote: All samples were blocked for non-specific binding with human FcR Blocking Reagent (Miltenyi Biotec, cat.: 130-059-901) for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were then incubated with conjugated antibodies (FITC-CD11b, BD Biosciences, #557396; PE-Recombinant-ACSA2, Miltenyi Biotec, #130-116-244) for 45 min at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: The assessment of expanded surface protein markers on sEV was carried out using a MACSPlex human Exosome kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) following the manufacturer’s protocol.44 Thirty seven distinct surface marker antibodies (CD1c ...
-
bioRxiv - Neuroscience 2024Quote: ... The tissue was dissociated into a single cell suspension and the debris were removed using the Adult Brain Dissociation Kit (Miltenyi Biotec, 130-107-677) and gentleMACS program 37C_ABDK_02 according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... The cell suspension was then processed through an LD column (Miltenyi Biotec, 130-042-901), and the follow-through was collected ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and 10 μl of APC-Microfold (130-102-149, Miltenyi Biotec)) and incubated for 30 minutes at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... and then passed through 30 and 20 μm filters (Miltenyi Biotec). The filtered cell suspension was centrifuged for 5 min at 300x g ...