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Citations for Miltenyi Biotec :
451 - 500 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... using the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec Cat. No. 130096427) and the pre-programmed protocol 37C_m_TDK_1 ...
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bioRxiv - Immunology 2024Quote: ... in gentleMACS C tubes (Miltenyi Biotec Cat. No. 130093237) using the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec Cat ...
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bioRxiv - Immunology 2024Quote: ... CD14+ microbeads (Miltenyi Biotec) were used to select monocytes according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... a section of frozen tissue was placed into a gentleMACS C Tube (Miltenyi Biotec, 130-093-237) with 2 ml of TST buffer ...
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bioRxiv - Genomics 2024Quote: ... Tissue was immediately dissociated using a gentleMACS Dissociator (Miltenyi Biotec, 130-096-427) using the m_Spleen_01_01 program twice and incubated on ice for 5 min to complete a 10 min incubation in Tween with salts and Tris (TST ...
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bioRxiv - Immunology 2024Quote: ... CD66+ granulocytes were isolated from the PBMC and PMN fractions using the CD66abce MicroBead Kit (Miltenyi Biotec, Germany) with an MS column ...
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bioRxiv - Immunology 2024Quote: ... Neutrophils were isolated by positive selection using Anti-Ly-6G MicroBeads UltraPure (Miltenyi Biotec; Auburn, CA, USA) and resuspended in RPMI 1640.
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bioRxiv - Immunology 2024Quote: ... lungs were homogenized in PBS using gentleMACS (Miltenyi Biotec, Auburn, CA, USA), then stained with a modified Diff-Quik staining (44 ...
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bioRxiv - Immunology 2024Quote: ... falciparum infected erythrocytes using a MACS cell separator with LD columns (Miltenyi Biotec #130-042-901), and diluted to 10 × 106 infected erythrocytes per mL in PBS ...
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bioRxiv - Immunology 2024Quote: Single cells suspension from tumour-bearing lungs described previously were used for CD8+ T cells sorting using immunomagnetic sorting using CD8α+ T cell isolation kit (Miltenyi Biotec) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... total CD11c+ cells were isolated from tumor-bearing lungs using CD11c microbeads (Miltenyi Biotec) and Venus+ cells were sorted ...
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bioRxiv - Immunology 2024Quote: ... The remaining submucosal tissue was placed in a conical tube containing fresh RPMI-1640 supplemented with digestion enzymes from the human Tumor Dissociation Kit (Miltenyi Biotec, #130-095-929) and chopped into small pieces using dissection scissors ...
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bioRxiv - Microbiology 2024Quote: ... Primary CD4+ T-cells and monocytes were purified from PBMCs by positive selection using CD4 (Miltenyi Biotec; 130-045-101) and CD14 (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... CD8- thymocytes were obtained by negative selection using anti-CD8 (Ly-2) MicroBeads (Miltenyi Biotec). The cells were then stained with fluorescently labeled antibodies to CD4 ...
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bioRxiv - Immunology 2024Quote: ... CD14+/CD16+ monocytes were isolated by negative selection magnetic bead column purification (Pan Monocyte Isolation Kit, Miltenyi Biotec). For chromatin accessibility (ATAC-seq) ...
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bioRxiv - Immunology 2024Quote: ... F4/80-FITC (Miltenyi Biotec), and Ly6C-Pacific blue (MACS) ...
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bioRxiv - Immunology 2024Quote: ... IFN-α (LT27:295, Miltenyi Biotec). PI (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Fluorescence was detected by a MACS Quant Analyzer (Miltenyi Biotec) or a FACSLyric Analyzer (BD Pharmingen) ...
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bioRxiv - Immunology 2024Quote: ... cells were washed twice by centrifugation with PBS with 0.04% bovine serum albumin (BSA; Miltenyi Biotec), then lysed with 45 µl chilled Lysis Buffer ...
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bioRxiv - Immunology 2024Quote: ... and human Treg expansion kit anti-CD3/CD28 beads (Miltenyi Biotec/Invitrogen), using x-vivo 15 cell medium (Lonza ...
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bioRxiv - Immunology 2024Quote: ... then followed by another incubation with anti-APC Microbeads (Miltenyi Biotec, #130-090-855). Negative enrichment to remove red blood cells ...
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bioRxiv - Immunology 2024Quote: IFN-γ production of CD8+ T cells was measured by adapting a commercially available IFN-γsecretion assay kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Immunology 2024Quote: ... followed by incubation of anti-APC beads (Miltenyi Biotec, cat# 130-090-855) at 4 °C for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... resuspended at 4 mio cells/ml and then cultured with HLA class I-enhanced neurons (see Human-iPSC-derived neuron preparation section above) at a density of 1.2mio cells/cm2 with IL-2 (1000UI/ml, Miltenyi Biotec) and a CD28 agonist antibody (5ug/ml ...
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bioRxiv - Immunology 2024Quote: ... cell medium was replaced with fresh medium supplemented with IFN-γ1b (1ng/ml, Miltenyi Biotec) and TNFα (1ng/ml ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells positively sorted by MACS (Miltenyi Biotec) were assessed for viability by AO/PI staining and counted with a Luna-FX7TM Automated cell counter (Logos Biosystems).
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bioRxiv - Immunology 2024Quote: Isolation of untouched CD8+ T cells from total PBMCs was performed using a two-step human CD8+ T cell isolation kit (Miltenyi Biotec). All steps were performed following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: Single-cell suspensions were blocked with FcR Blocking Reagent (Miltenyi Biotec) and stained with cocktails of fluorochrome-conjugated antibodies ...
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bioRxiv - Immunology 2024Quote: ... NKG2C+ NK cells were then selectively isolated as aNK cells using anti-PE microbeads (Miltenyi Biotec), while the flow-through ...
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bioRxiv - Immunology 2024Quote: ... we initially isolated bulk NK cells following the manufacturer’s guidelines (Miltenyi Biotec). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulation was induced with anti-CD3ε and anti-CD28 beads (T Cell Activation/Expansion Kit mouse, Cat.#130-093-627, Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were prepared from normal BL/6 spleens using a CD4+ T Cell Isolation Kit II for mouse (Miltenyi Biotec, Cambridge, MA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cells were stimulated with either PepTivator® SARS-CoV-2 Prot_S-peptide pool (Miltenyi Biotec) or S recombinant protein (Sino Biological ...
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bioRxiv - Immunology 2024Quote: ... PepTivator® SARS-CoV-2 Prot_S-peptide pool (Miltenyi Biotec, consisting of 15-mer sequences with 11 amino acids overlap covering the complete protein coding sequence (aa 5–1273 ...
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bioRxiv - Immunology 2024Quote: ... OT-I CD8 T (OT-I) cells were enriched by negative selection using a CD8a+ T cell isolation kit (Miltenyi Biotec), commonly reaching a purity of 90-98% as determined by flow cytometry (data not shown) ...
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bioRxiv - Immunology 2024Quote: ... Fluorescence was detected by the MACS Quant Analyzer (Miltenyi Biotec) or FACS Lyric (Becton Dickinson ...
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bioRxiv - Immunology 2024Quote: ... 30 ng/ml anti-CD3 antibody (OKT-3) (Miltenyi Biotec) and 3000 IU/ml hrIL-2 as previously reported12 ...
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bioRxiv - Immunology 2024Quote: ... Fluorescence was detected by a MACS Quant Analyzer (Miltenyi Biotec) or a FACSLyric Analyzer (BD Pharmingen) ...
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bioRxiv - Immunology 2024Quote: ... The cells were further purified with magnetic beads and LS Columns from a Mouse neutrophil isolation kit (Miltenyi Biotec 130-097-658) resulting in 97-99% purity as determined by flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... B cells and T cells (Miltenyi Biotec) were used ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were negatively selected from the minced auricular lymph nodes using the CD4+ T cell isolation kit (Miltenyi Biotec) and cultured in RPMI 1640 media supplemented with 25 mM HEPES ...
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T-cell development and activation in humanized mice lacking mouse major histocompatibility complexesbioRxiv - Immunology 2024Quote: ... CD34+ cells were positively selected through two consecutive runs using immune magnetic beads kits (Miltenyi Biotec, Bergisch-gladbach, Germany) and cryopreserved ...
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bioRxiv - Genomics 2024Quote: ... CD73 negative neuronal cells were enriched by autoMACS Pro Separator (Miltenyi Biotec) DEPLETES mode ...
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bioRxiv - Immunology 2024Quote: ... Lung lobes were excised and dissociated in C tubes (Miltenyi Biotec) in HEPES buffer containing Liberase Blendzyme 3 (70 mg/ml ...
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bioRxiv - Immunology 2024Quote: ... and depleted of mature T cells using a CD3ε depletion kit (Miltenyi Biotec, #130-094-973). 1-2×106 bone marrow cells were then injected retro-orbitally to each host mouse under isoflurane anesthesia ...
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bioRxiv - Immunology 2024Quote: ... containing 1mL PBS+0.05% Tween-80 (PBS-T) using a GentleMACS tissue dissociator (Miltenyi Biotec). Organ homogenates were then diluted in PBS-T and aliquots were plated onto 7H10 agar media to quantify Mtb burden ...
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bioRxiv - Immunology 2024Quote: Mouse lung lobes and spleens were homogenized in M tubes (Miltenyi Biotec) containing 1mL PBS+0.05% Tween-80 (PBS-T ...
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bioRxiv - Immunology 2024Quote: ... CD14+ human monocytes were isolated from fresh peripheral blood mononuclear cells (PBMCs) using CD14 MACS MicroBeads (Miltenyi Biotec, Cat. No. 130-050-201) and cultured in MoDC differentiation medium at a density of 106 cells/ml for 6 days ...
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bioRxiv - Immunology 2024Quote: ... Percentage of monocytes and immature MoDC cells were analyzed in a MACSQuant® flow cytometer (Miltenyi Biotec). Flow cytometry data were analyzed using FlowJo [version 10.0.7 (TreeStar)].
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bioRxiv - Immunology 2024Quote: ... Percentage of transfected cells was analyzed in a MACSQuant® flow cytometer (Miltenyi Biotec). CD14+ monocytes and inmature MoDC were frozen in fetal bovine serum (FBS ...