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Citations for Miltenyi Biotec :
3001 - 3039 of 3039 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... iPS cells were seeded on previously coated Laminin 521 (Biolamina, Sundbyberg, Sweden) plates to support cell viability in StemMACS™ iPS-Brew XF (Miltenyi Biotec, Bergisch Gladbach, Germany) supplemented with 10 µM of Rock inhibitor Y-27632 and 1x CloneR (Stemcell Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... CD146+ pericytes were isolated from digested cell suspensions of Scx+ or ScxGFP mice using anti-CD146 magnetic microbeads (130-092-007, Miltenyi Biotec, San Diego, CA, USA), and placed into 24-well tissue culture dishes (Corning ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated from C57BL/6 mice (male, 6-12 weeks old) and enriched using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, No:130-104-453). Quality control was performed by flow cytometry (CD4+ [clone RM4-5 ...
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bioRxiv - Immunology 2021Quote: ... CD45− cells were enriched by autoMACS separation after magnetic labeling of CD45+ cells using anti-CD45-APC followed by anti-APC microbeads (Miltenyi Biotec, Cat. #130-090-855) or anti-CD45 Nanobeads (Biolegend ...
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bioRxiv - Molecular Biology 2022Quote: Healthy and CLL splenic B cells were isolated from one-year-old WT and Eμ–TCL1 mice by positive B-cell selection with CD19 magnetic beads (CD19 MicroBeads, Miltenyi Biotec, cat. 130-121-301). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... and then the cell pellet was resuspended in 100 µL of MACS buffer and 25 µL of anti-integrin α-7 microbeads (Miltenyi Biotec, #130-104-261) for incubation at 4 °C for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... Ly6G+ cells were positively selected to isolate G-MDSCs from Ly6G- M-MDSCs and were passed through LS columns (Miltenyi Biotec, cat. 130-042-401) twice to increase purity ...
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bioRxiv - Immunology 2020Quote: ... BM cells were incubated on ice for 20 minutes with c-Kit microbeads (Miltenyi Biotec, 130-091-224; 5 μl/100 μl SM per mouse), washed with SM and enriched on an AutoMACS Pro magnetic cell separator (Miltenyi Biotec) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were isolated from mouse lung tissue (without the trachea) using the QuadroMACS™ Separator and antibody-bound magnetic beads (Miltenyi Biotec, Leiden, The Netherlands). EpCAM+ cells and fibroblasts were mixed 1:1 (20,000 cells each ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were washed 3x with MACS Buffer (autoMACS Rinsing Solution [Miltenyi Biotec, 130-091-222] with MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376]). After removing supernatant from final wash ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampi tissues were dissociated to single-cell suspension by enzymatic degradation using a MACS Technology neural tissue dissociation kit (Miltenyi Biotec, Cat# 130-092-628) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The brain tissues from two animals were pooled in every group and processed for live single-cell suspension preparation using gentleMACS™ Tissue Dissociator (Miltenyi Biotec, Gaithersburg, MD, USA). The cell suspensions were incubated with CD11b microbeads (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... the donor liver is chopped into small pieces before undergoing enzymatic digestion and CD133 selection via magnetic activated cell sorting (Miltenyi Biotec, Cat#170-076-719). Primary human HPC are routinely expanded as 3D organoids suspended in Matrigel (Corning ...
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bioRxiv - Immunology 2023Quote: ... target U937 cells (killing-sensitive) and control K562 cells (killing-resistant) were pre-treated with soluble anti-CD3 monoclonal antibody (mAb; OKT3, Miltenyi Biotec, at 200 ng/ml) for 15 min at 37°C prior to co-culture with primary human CD8+ T cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cut along the coronal plane with a razor into three pieces and digested into cell suspensions using a Multi Tissue Dissociation kit 1 (Miltenyi Biotec, Germany; #130-110-201) and a gentleMACS™ OctoDissociator with heaters (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2022Quote: ... and lungs were processed into single-cell suspensions with a gentle MACS™ Octo Dissociator and Mouse Lung Dissociation Kit (Miltenyi Biotec, #130-095-297) according to manufacturer protocols.
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bioRxiv - Immunology 2023Quote: ... T cells and low-density granulocytes were depleted with anti-CD3 and anti-CD15 microbeads using a QuadroMACS separator (Miltenyi Biotec, Cat. 130-090-976) and LS columns (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... the pellet was incubated with RBC lysis buffer for 5 min on ice to remove red blood cells (RBC) and then used for Treg isolation with a CD4+CD25+ Treg isolation kit (Miltenyi Biotec, cat # 130-091-041), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... Neurospheres and attached cells were resuspended in scRNA-seq buffer (HBBS + 0.04% BSA, Fisher Scientific #14-175-095 and Miltenyi Biotec #130-091-376, respectively) and washed 3 times ...
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bioRxiv - Cancer Biology 2023Quote: ... a fixed number of cells were used to start the TCT program on Prodigy using a TS520 tubing set (Miltenyi Biotec, Cat# 170-076-600). Cells were cultured in Optimizer medium (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were positively selected using anti-CD4 and CD8 antibodies through the Prodigy TCT program on a CliniMACS Prodigy (Miltenyi Biotec, Cat# 200-075-301). After selection ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were resuspended in 500 μl of MaCSQuant® Tyto® running buffer and analysed with MACSQuant® Tyto® Cell Sorter (Miltenyi Biotec).
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bioRxiv - Microbiology 2023Quote: MEFs were seeded at 5.104 cells per well in 24-well plates one day before stimulation and treated with 300 IU/mL IFNα (Miltenyi Biotec Cat#130-093-131) and incubated for up to 24 hours.
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bioRxiv - Biophysics 2024Quote: Primary human NK cells from healthy donors were isolated from blood samples provided by the EFS (Marseille, France) by negative selection using the MACSxpress Whole Blood human NK cell isolation kit (Miltenyi Biotec cat. 130-098-185), according to the manufacturer protocol ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... PBMCs were stimulated for 3 hours with 10 μg/mL of Gag AK11 peptide and T cells producing IFN-γ in response were enriched using the IFN-γ Secretion Detection and Enrichment Kit (130-054-201; Miltenyi Biotec, Bergisch Gladbach, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Naïve mouse B-cells were isolated from the spleen of 7–10 weeks old wild type or c-mycf/f mice with the B-cell isolation kit (MACS Miltenyi Biotec Cat. no. 130-090-862). After purification ...
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bioRxiv - Neuroscience 2020Quote: ... The cell pellet was gently re-suspended in 3100 μl cold D-PBS and processed for additional removal of myelin and cell debris with Debris Removal Solution (Miltenyi Biotec, cat. no. 130-109-398), following manufacturer’s instructions ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... CD4+ and CD8+ T-cells were isolated from spleens using CD4+ or CD8+ positive selection according to the manufacturer’s protocol (Miltenyi Biotec, # 130-117-043, 130-117-044). T-cells were resuspended in UCM ...
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bioRxiv - Cell Biology 2023Quote: ... to enrich immune cells in the CD45 positive fraction and further loaded the CD45 negative fraction onto CD31 MicroBeads (Miltenyi Biotec catalog number 130-091-935). We collected the CD31 positive fraction to enrich endothelial cells and also collected and loaded the CD31 negative fraction ...
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bioRxiv - Cancer Biology 2023Quote: ... holoclones and non-holoclones were divided and sorted by flow cytometry for stem-like cells following CD44 staining with CD44 antibody (Miltenyi Biotec Cat no 130-095-180). Sorted CD44high holoclones were used to isolate RNA to quantify stem cell marker expression.
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bioRxiv - Immunology 2023Quote: ... Click-iT® Plus Alexa Fluor® Picolyl Azide assay was then performed and cells were analyzed on MACSQuant® VYB Cytometer (Miltenyi Biotec, SFR BioSciences).
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bioRxiv - Cancer Biology 2024Quote: ... Mouse prostate tumor and normal tissues were dissociated for single cell RNA sequencing using the Tumor Dissociation kits from Miltenyi Biotec (cat. no. 130-096-730). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were harvested by centrifugation at 400g for 10 min and resuspended in chilled 1X Red Blood Cell Removal Solution (Miltenyi Biotec, cat. no.130-094-183). Debris was then removed using the Debris Removal Solution (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: Pineal tumors from IPDrosha/IPDicer1 mice were dissociated into single-cell suspension using the Brain Tumor Dissociation Kit (P) (Miltenyi Biotec, cat. no. 130-095-942), and then “Cleavage Under Targets and Release Using Nuclease” (CUT&RUN ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells were isolated from the spleens of uninfected or MHV-A59 infected animals using the untouched CD8+ T cell isolation MACS kit from Miltenyi Biotec (cat#130-104-075, Gaithersburg, MD) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was coupled with Anti-tomm22 micro-Beats (30min at 4° C) to remove free mitochondria through Magnetic-assisted cell sorting (MACS) (Miltenyi Biotec, 130-096-946, 130-042-401). The mitochondria-depleted flow-through was centrifuged at 13,000 × g for 10 min at 4° C to pellet synaptosomes (100ul of PBS resuspension ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were suspended in a 0.5% NGS solution then incubated with anti-PDGFRα or anti-O4 coupled-beads (Miltenyi Biotec; ref 130-094-543 and 130-096-670). Unbound bead-coupled antibodies were washed away by centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... antibody for 45 min at 4°C followed by the incubation with the secondary antibody anti-IgG1-PE (REA1017, Miltenyi Biotec, 1 μL/105 cells in 100 μL) for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples at the 4 hour activation time point were also processed to isolate CD4+ and CD8+ T cells by positive CD4 and CD8 selection kits (Miltenyi Biotec, Cat# 130-045-101 and 130-045-201). Unstimulated T cells were processed at the 0 hour time point without any activation ...