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Citations for Miltenyi Biotec :
2601 - 2650 of 3163 citations for Primary Human Arterial Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... These cells were mixed with CD4+ beads and separated via MACS LS Columns (both Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: The isolated NK cells were maintained in the NK MACS Basal medium supplemented with NK MACS supplement (Miltenyi Biotec), 5% human AB serum (Sigma) ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were pre-enriched from spleens of CD45.1 or CD45.1/2 OT-II mice by negative selection using the CD4+ T-cell isolation kit (Miltenyi Biotec). Live (SytoxBlue−)CD25− CD44loCD62L+TCRβ+vα2+ naïve OT-II T cells were FACS-isolated ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were purified by incubation with anti-CD4 microbeads and passing through a magnetic column (Miltenyi Biotec). The purity of cells was higher than 95% as determined by flow cytometry ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed twice with PBS 2% FCS before analysis by flow cytometry using a MACSQuant cytometer (Miltenyi Biotec, Germany) and analysis was done with the software FlowJo (vers 7.6).
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bioRxiv - Immunology 2021Quote: ... Total T cells from spleen of Fam13a KO mice and WT littermates were isolated with CD90.2 microbeads (130-121-278, Miltenyi Biotec). The cells were then stained with the antibody mix containing LIVE/DEAD® Fixable Near-IR (dilution 1:500) ...
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bioRxiv - Cell Biology 2020Quote: Lineage negative and CD31 positive BMCs were isolated using first immunomagnetic Lineage Cell Depletion Kit (130-092-211, Miltenyi Biotec) followed by positive selection with CD31 MicroBead Kit (130-091-935 ...
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bioRxiv - Cell Biology 2020Quote: ... Naïve CD4+ T cells were isolated from the lymph nodes and spleen of C57BL/6 mice by MidiMACS (Miltenyi Biotec). Cells were activated by plate-bound anti-CD3 (5 μg mL−1 ...
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bioRxiv - Developmental Biology 2021Quote: ... and seeded at a density of 19,000 cells/cm2 on Matrigel in mTeSR1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y-27632 (Miltenyi Biotec). Upon reaching 95% confluence ...
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bioRxiv - Immunology 2021Quote: Naive CD4+ or CD8+ T cells were positively selected from single-cell suspensions prepared from spleen and lymph node by mechanical disruption using streptavidin MicroBeads according to manufacturer’s instructions (Miltenyi Biotec). Purified cells were activated in twelve-well plates coated with 0.1-10 μg/mL αCD3 (145–2C11 ...
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bioRxiv - Neuroscience 2021Quote: ... ACSA-2 labelled cells were flushed from the MS column with pre-warmed AstroMACS medium (Miltenyi Biotec, 130-117-031) supplemented 50 U/ml penicillin/streptomycin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Red blood cells and debris were removed and astrocytes magnetically labelled using ACSA-2 microbeads (Miltenyi Biotec, 130-097-678) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: CD4+ cells were isolated from C7 Tg mouse spleens using mouse CD4 (L3T4) MicroBeads from Miltenyi Biotec (130-117-043) according to the manufacturer’s protocol and cultured in T cell media at 2 x 106 cells/mL with 1 μM ESAT-6 peptide pool (NIH BEI Resources Repository catalog no ...
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bioRxiv - Immunology 2020Quote: ... T cells were purified with either anti-CD8 or anti-CD4 magnetic beads using MACS columns (Miltenyi Biotec, Auburn, CA) prior to cell surface staining ...
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bioRxiv - Systems Biology 2019Quote: ... Depletion of undesired cell types was achieved by the use of microbeads specific for APC (Miltenyi Biotec, 130-090-855), which ensured magnetic retention of these cells ...
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bioRxiv - Genomics 2020Quote: ... and CD138+ multiple myeloma cells isolated from bone marrow biopsies by positive selection on antibody-conjugated magnetic beads (Miltenyi Biotec), were obtained with I.R.B ...
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bioRxiv - Genomics 2020Quote: ... CD34 positive cells were then bead enriched using the CD34 MicroBead Kit UltraPure (Miltenyi Biotec, catalog number 130-100-453) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... for an additional 15 min and subsequently subjected to positive selection of Gr-1+ cells on a MACS LS column according to the manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... Lungs from infected mice were digested using the Miltenyi lung cell isolation buffer and dissociated via GentleMACS (Miltenyi Biotec, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... kit with papain and double cell isolation using magnetic coupled antibodies anti-CD11B (Microglia) according to the manufacturer’s protocol (Miltenyi Biotec) and as previously described27 ...
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bioRxiv - Neuroscience 2020Quote: ... Single cell suspensions were generated using the Neural Tissue Dissociation Kit – Postnatal Neurons (Miltenyi Biotec. Cat no. 130-094-802), following manufacturer’s instructions for manual dissociation ...
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bioRxiv - Neuroscience 2020Quote: ... Cell suspension was obtained by automated dissociation using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Cat#130-092-628) and the gentleMACS™ Dissociator (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2021Quote: ... and the cell pellet was gently re-suspended in Red Blood Cell Lysis Solution (freshly prepared according to manufacturer’s instruction; 130-094-183; Miltenyi Biotec B.V ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ and CD8+ T cells were isolated by MACS from monocyte-depleted PBMC using CD4 and CD8 microbeads (Miltenyi Biotec). These primary cells were cultured in X-vivo 15 (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... T cells from the lungs of naïve or infected mice (day 8 pi) were enriched using the Pan T cell Isolation Kit II (Miltenyi Biotec) and co-cultured with infected BMDC at a ratio of 10:1 for 2-3 hours followed by the addition of Brefeldin A (5µg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... at 37°C for 1 hr and passed through a 70 μm and a 30 μm cell strainer (Miltenyi Biotec). CD45+ cells were isolated using magnetic beads (Miltenyi Biotec 130-052-301 ...
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bioRxiv - Cancer Biology 2022Quote: ... Single mouse tumor cell suspensions were obtained using a mouse Tumor Disassociation Kit from Miltenyi Biotec (Cat# 130-096-730). After leukocytes extraction using density gradient medium ...
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bioRxiv - Molecular Biology 2022Quote: ... The brain was dissociated into single cell suspension using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) according to the manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: ... total CD4+ T cells were directly isolated from buffy coats using the StraightFrom Buffy Coat CD4 MicroBead kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Primary NK cells were isolated and enriched the day of the experiment from the spleens of 8-12 week old female C57BL/6 mice using the NK Cell Isolation Kit II according to manufacturer’s instructions (Miltenyi Biotec). 50,000 NK cells were then seeded in the top chamber of a transwell insert (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... then astrocytes were isolated using the Miltenyi Biotec’s anti-ACSA-2 magnetic cell sorting kit and protocol (Miltenyi Biotec, 130097678). RNA was purified and concentrated with proteinase K cell digestion ...
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bioRxiv - Immunology 2022Quote: ... PMEL T cells were isolated from the splenocytes of the Thy1.1+ PMEL TCR transgenic mice by CD8a+ negative selection (Miltenyi Biotec). Each irradiated Krt14-Kitl* mouse received 1×106 cells of PMEL plus 1.8×106 cells of the peptide-pulsed MHC-II+ ...
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bioRxiv - Immunology 2022Quote: Neutrophils were purified from freshly isolated mouse bone marrow cell suspensions using an autoMACS pro-selector cell separator with a MACS neutrophil isolation kit for negative selection in accordance with the manufacturer’s instructions (Miltenyi Biotec). Neutrophils were aged overnight in serum-free medium at 3 × 106 cells/ml at 37 °C and 5 % CO2 ...
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bioRxiv - Immunology 2022Quote: ... Next the cells were washed and incubated for 20 min at 4°C with 100μl anti-FITC MACS magnetic beads (Miltenyi Biotec) per 108 cells ...
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bioRxiv - Cell Biology 2022Quote: ... Fresh tumor tissue from NSCLC patients were enzymatically digested and single-cell suspensions were stained with CD14 microbeads (Miltenyi Biotec) and positively selected using AutoMACS ProSeparator (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was dissociated into single cell suspension using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) according to the manufacturers protocol ...
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bioRxiv - Immunology 2020Quote: Splenic B cells of both genotypes were CD43-depleted using magnetic beads (130-049-801, Miltenyi Biotec, Bergisch Gladbach Germany) and taken in culture in the presence of 1μg/ml anti-CD40 antibody (102901 ...
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bioRxiv - Immunology 2020Quote: ... Tregs were purified after enrichment of CD25+ cells using biotinylated anti-CD25 mAb (7D4) and anti-biotin microbeads (Miltenyi Biotec), followed by CD4 staining (RM4.5 ...
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bioRxiv - Microbiology 2021Quote: ... CV38-142 IgG used in the SPR assay was produced in CHO cells and was kindly provided by Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Cell Biology 2020Quote: ... On day 15 of cardiac differentiation fixed cells were labeled for flow cytometry using alpha-actinin (Miltenyi Biotec, Cat.#130106937) and corresponding isotype control ...
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bioRxiv - Microbiology 2020Quote: ... For the isolation of pDCs from PBMCs the Plasmacytoid Dendritic Cell Isolation Kit II from Miltenyi Biotec (# 130-097-415) was used ...
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bioRxiv - Neuroscience 2021Quote: ... Positive selection of NeuN+ cells was then performed according to the standard MACS MS Columns protocol available from Miltenyi Biotec. After the first round of magnetic separation ...
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bioRxiv - Neuroscience 2022Quote: ... Motoneurons were isolated from the spinal cord of Smn2B/+ and Smn2B/- embryos using 5.2% iodixanol density gradient centrifugation combined with p75-based magnetic cell isolation (Miltenyi Biotec) as we previously described (Soulard et al. ...
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bioRxiv - Developmental Biology 2022Quote: A dissociated single-cell suspension was passed through 30 µm nylon mesh (Pre-Separation Filters; Miltenyi Biotec #130-041-407) to remove cell clumps that could clog the column ...
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bioRxiv - Developmental Biology 2022Quote: ... Each purified hepatocyte pellet was resuspended in 5ml of red blood cell (RBC) lysis solution (Miltenyi Biotec 130-094-183) and incubated at room temperature for 10 mins ...
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bioRxiv - Immunology 2022Quote: ... CD4 T cells from HLA-DRb1*04-positive donors were magnetically purified using CD4 microbeads according to the supplier’s instructions (Miltenyi Biotec), while CD3+/CD4+/CD8αlow/CCR6+/CXCR6+ F ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 μl of cell suspension was incubated with CD117-APC (c-KIT) antibody (Miltenyi Biotec, 130-098-207, 1:50), CD133-PE (PROM1 ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatant containing NPCs was counted and Kupffer cells (KC) were removed using anti-F4/80 Ultrapure microbeads (Miltenyi Biotec) following manufacturer’s guidelines ...
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bioRxiv - Immunology 2019Quote: ... before culturing in a 1:10 ratio with 5×105 BALB/c splenic APCs (prepared via Thy1.2+ cell depletion by MACS, Miltenyi Biotec) in a 96-well round-bottom plate in complete RPMI medium for 5 days ...
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bioRxiv - Immunology 2020Quote: ... then the cells were separated into bound and non-bound fractions on an autoMACS™ separator column (Miltenyi Biotec GmbH). The purity of the isolated cells ...