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Citations for Miltenyi Biotec :
5201 - 5250 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). CD11b+ cells were Fc blocked with α-CD16/32 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... PE CD158a/h (KIR2DL1/DS1) anti-Human antibody: 130-099-209 from Miltenyi Biotec. APC CD158b1/b2j (KIR2DL2/DL3/DS2 ...
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bioRxiv - Immunology 2022Quote: ... The Fc blocked cells were stained with 1:200 diluted anti-human CD45-APC (Cat. #130-113-114, Miltenyi Biotec) for 20 min at 4 degree Celsius in dark ...
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bioRxiv - Immunology 2022Quote: ... and then depleted with the anti-biotin Microbeads Ultrapure (Miltenyi Biotec, 130-105-637). Enriched B cells were finally sorted by DAPI ...
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bioRxiv - Immunology 2022Quote: ... CD45+ cells were positively selected using anti-CD45 TILs microbeads (Miltenyi Biotec). The frequency of Tregs infiltrating in the tumor microenvironment was defined as FOXP3+CD25+CD4+ among CD45+ live cells and assessed by flow cytometry ...
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bioRxiv - Immunology 2022Quote: Engineered mouse B cells were analyzed by double-staining with FITC labeled anti-mouse IgM antibody (Miltenyi Biotec, 130-095-906) and a SOSIP protein labeled with APC using a fluorochrome using the Lightning-Link (R ...
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bioRxiv - Immunology 2022Quote: ... mouse B cells were first isolated from spleen and lymph nodes using the Pan B-cell Isolation Kit II (Miltenyi Biotec,130-104-443) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... anti-CD19 or anti-CD14 microbeads (Miltenyi Biotec). Enriched cells were then sorted as live CD45+ CD3+ CD4+ or CD45+ CD3+ CD8+ or CD45+ CD3neg CD19+ or CD45+ CD3neg CD14+ using FACS ARIA.
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bioRxiv - Immunology 2022Quote: ... tumors from unimmunized or mice immunized with WTL+LPS+PGPC were harvested then dissociated using the tumor Dissociation Kit and the gentleMACS dissociator (Miltenyi Biotec), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and monocytes were then enriched using anti-Human CD14 beads (Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were cultured with 100 ng/mL (500 UI/mL) GM-CSF plus 50 ng/mL (250 UI/mL) IL-4 (Miltenyi Biotec) at 106cells/mL in complete RPMI medium supplemented with 10% FCS (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... and LS columns (Miltenyi Biotec, 130-042-401).
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bioRxiv - Immunology 2022Quote: ... and LS columns (Miltenyi Biotec, 130-042-401). Before electroporation ...
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bioRxiv - Immunology 2022Quote: ... mice were sacrificed and B cell were isolated from spleen and lymph nodes with mouse Pan B Cell Isolation Kit II (Miltenyi Biotec, 130-104-443) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... and magnetically-labeled anti-PE beads (Miltenyi Biotec). Naive CD4+ T cells were always more than 98% pure ...
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bioRxiv - Immunology 2022Quote: ... Data was collected using the MACSQuant10 Analyzer flow cytometer (Miltenyi Biotec) equipped with a 405nm (violet) ...
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bioRxiv - Immunology 2022Quote: ... Recombinant human transforming growth factor β1 (premium grade, Miltenyi Biotec) was diluted and used at the indicated concentrations.
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bioRxiv - Immunology 2022Quote: ... cells were washed with 2% FBS containing PBS and incubated with 1:20 FcR blocking reagent (Cat. #130-059-901, Miltenyi Biotec) in 2% PBS for 10 min at RT ...
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bioRxiv - Immunology 2022Quote: ... tissues were either freshly processed or transiently stored overnight MACS Tissue Storage solution (Cat. #130-100-008, Miltenyi Biotec) at 4 degree Celsius (in case of delayed surgeries ...
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bioRxiv - Genomics 2022Quote: ... The sample was then macrodissected and dissociated into a cellular suspension by the gentleMACS Octo Dissociator using the human tumor dissociation kit as per manufacturer’s recommendations and the 37C_h_TDK_3 program (Miltenyi Biotec, Bergisch Gladbach, Germany). The suspension was used for scRNA-seq ...
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bioRxiv - Immunology 2022Quote: ... The incubated cells were then sorted with LS magnetic beads (130-042-401, Miltenyi Biotec, Germany), washed with PBS and collected mononuclear cells for reserve ...
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bioRxiv - Immunology 2022Quote: ... Gently absorbed mononuclear cell layer was resuspended in PBS to centrifuge at 500g for 5min and the resuspended cell pellets were incubated with CD3 Microbeeds (130-050-101, Miltenyi Biotec, Germany) at 4-8°C for 15min ...
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bioRxiv - Immunology 2022Quote: ... Cell suspension was diluted with warm complete culture medium and incubated in a continuous motion using the MACSmix rotator (Miltenyi Biotec) for 45 min at 37°C (5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... 500 IU/ml IL-2 and 10 ng/ml IL-15 (all the above regents were from Miltenyi Biotec). The above transfection mix was added into NK cell culture seeded in six-well plates (3×106/well ...
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bioRxiv - Immunology 2022Quote: ... minced and digested using the Tissue Dissociation Kit 1 and a gentleMACS™ Dissociator (both from Miltenyi Biotec). Cells were filtered through a 70 μm cell strainer ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were positively selected with CD4 (L3T4) magnetic microbeads (Miltenyi Biotec, Auburn, CA), pooled and suspended in PBS ...
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bioRxiv - Immunology 2022Quote: We stimulated the spleen and lung cells from immunized and control animals to detect nucleocapsid protein-specific CD8+ T cell responses with an N protein-peptide pool at a concentration of 1.5 μg/mL/peptide (Miltenyi Biotec; 130-126-699, Germany) at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029 ...
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bioRxiv - Immunology 2022Quote: ... tissue was homogenized in Qiazol lysis reagent using a gentleMACS Dissociator (Miltenyi Biotec, Germany) and filtered in an RNAeasy spin column (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... We stimulated splenocytes and lung cells (3 × 105) in vitro with either N-protein peptide pool (Miltenyi Biotec; #130-126-699, Germany) or S-protein peptide pool (Genscript ...
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bioRxiv - Immunology 2022Quote: ... or S-protein (B.1.351) mutation peptide pool (Miltenyi Biotec, # 130-127-958, Germany) at a concentration of 1.5 μg/ml/peptide at 37 °C for 16-18 h in pre-coated ELISpot plate (MSIPS4W10 from Millipore ...
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bioRxiv - Immunology 2022Quote: TILs were stimulated for 7 days with anti-biotin MACSiBead Particles loaded with biotinylated CD3/CD28 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany). The preparation of the conjugated beads was carried out according to the manufacture’s recommendation (activation/expansion kit ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were incubated for 72 hours at 37°C with either anti-CD3/anti-CD28 beads (1:2 bead-to-cell ratio, Miltenyi Biotec) or 200ng/mL N-803 in the presence of 50µM raltegravir and 5µM saquinavir ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were isolated from the PBMC by negative selection using nonhuman primate CD4 MicroBeads according to the manufacturer’s protocol (Miltenyi Biotec). CD4+ T cells were incubated for 72 hours at 37°C with either anti-CD3/anti-CD28 beads (1:2 bead-to-cell ratio ...
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bioRxiv - Immunology 2022Quote: ... Cells were subsequently washed in autoMACS running buffer (Miltenyi Biotec Inc.) and centrifuged at 500g for 5 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... ECs were purified by magnetic-activated cell sorting (MACS) using anti-CD146 MicroBeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from peripheral blood lymphocytes by negative selection using a CD4 T-cell isolation kit II and magnetic cell separation (Miltenyi Biotec). A total of 50,000 CD4+ T cells were cultured in IMDM (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were then purified from freshly isolated PBMCs using a CD14+ selection MACS purification (Miltenyi Biotec) followed by final re-suspension in chemotaxis buffer (Dulbecco’s modified Eagles medium (DMEM ...
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bioRxiv - Immunology 2022Quote: ... The preparation of the conjugated beads was carried out according to the manufacture’s recommendation (activation/expansion kit, Miltenyi Biotec). Antibody-loaded anti-biotin MACSiBead™ were added to the TIL at a cell/bead ratio of 1:1 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and anti-cMyc-FITC (1:50, Miltenyi Biotec, Somerville, MA, #130-116-485) at 4°C for 45 min in the dark ...
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bioRxiv - Genomics 2022Quote: 3ug of biotinylated RNA and 60pg of in vitro transcribed spike-in RNA (ERCC-00048) in 200ul water was mixed with 100ul of beads from the uMACS Streptavidin kit (Miltenyi Biotec 130-074-101) on a thermoblock at 24°C at 800 RPM for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... and stimulated with T Cell TransAct (Diluted at 1:100, T Cell TransAct™, human, Miltenyi Biotec, #130-111-160), with fresh medium replaced every 3 days ...
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bioRxiv - Immunology 2022Quote: ... and B220 and anti-biotin MACS beads (Miltenyi Biotec). The purity of transgenic CD4+OT-II T cells was verified by flow cytometry (CD45.1+Vα2+Vβ5+CD25− ...
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bioRxiv - Genetics 2022Quote: ... or MACSxpress Neutrophil Isolation Kits human (Miltenyi Biotec) with an aim of 2 × 106 cells ...
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bioRxiv - Genetics 2022Quote: β-Thalassemia CD34+ HSPCs (Table S2) were enriched using the Miltenyi CD34 Microbead kit (Miltenyi Biotec). CD34+ HSPCs were thawed on day 0 into X-VIVO 15 (Lonza ...
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bioRxiv - Genetics 2022Quote: ... Human tonsil tissue was processed by mincing with scissors followed by transfer of up to 4g of tissue to a gentleMACS C tube (Miltenyi Biotec) containing 8 mL of phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2022Quote: ... Anti-biotin-PE antibody was purchased from Miltenyi Biotec (130-113-291) for biotin–LPETG SrtA substrate staining ...
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bioRxiv - Immunology 2022Quote: ... using gentleMACS (Miltenyi Biotec). The suspension was passed through a 100 μm cell strainer (Falcon) ...
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bioRxiv - Immunology 2022Quote: ... The cells were then stained with anti-human IgE FITC (IgE21; eBioscience) and anti-IgG1-PE human (IS11-3B2.2.3; Miltenyi Biotec) for 30 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2022Quote: ... CD8+ SP thymocytes were isolated using the CD8a+ (Ly-2) positive selection kit per manufacturer’s instructions (Microbeads, #130-117-044; Miltenyi Biotec). Flow-through resulted in the DN population ...
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bioRxiv - Immunology 2022Quote: ... Single cells were depleted from both the CD4+ double positive (DP) and CD4+ single positive (SP) cells using a CD4+ (L3T4) positive selection kit (Microbeads; #130-117-043; Miltenyi Biotec, Gaithersburg, USA) flow-through (this contains both double-negative [DN] and CD8+SP cells) ...