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Citations for Miltenyi Biotec :
1 - 50 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and subsequently CD4 microbeads for T helper cells (all Miltenyi Biotec, Bergisch Gladbach, Germany). The homogeneity of the cell preparations was controlled by flow cytometry.
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bioRxiv - Immunology 2024Quote: CD8+ T cells were isolated from the spleens of uninfected or MHV-A59 infected animals using the untouched CD8+ T cell isolation MACS kit from Miltenyi Biotec (cat#130-104-075, Gaithersburg, MD) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... anti-CD319 (all Miltenyi Biotec), anti-CD3 ...
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bioRxiv - Immunology 2024Quote: ... Flow cytometry was performed using MACSQuant (Miltenyi Biotec, Bergisch Gladbach, Germany) and data were analyzed with FlowJo software (FlowJo ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions of mouse lungs were generated by putting lungs in gentle MACS™ C tubes and mechanically homogenizing in 5 ml PBS using a gentle MACS™ Octo Dissociator (Miltenyi Biotec) in the absence of enzymes ...
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bioRxiv - Cancer Biology 2024Quote: ... EpCAM (FITC, Miltenyi Biotec 130-110-998) and PDPN (APC ...
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bioRxiv - Cancer Biology 2024Quote: ... Tissue homogenization was facilitated by use of the gentleMACS dissociator (Miltenyi Biotec, 130-093-235). Samples were then centrifuged at 4,000 x g for 5 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The purity of the NK cell population was validated by flow cytometry using PE-Vio 770 anti-human CD56 (1:50; REA196 clone; Miltenyi Biotec) and FITC anti-human CD3 (1:50 ...
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bioRxiv - Cancer Biology 2024Quote: ... The Human Tumor Dissociation Kit (Miltenyi Biotec 130095929) was used per the manufacturer’s protocol to generate a single-cell suspension from the tumor sample ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary NK cells were extracted from PBMCs by negative immunomagnetic isolation using a NK cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by loading onto a LS Separation column (Miltenyi Biotec). The cell suspension was then placed in the magnetic field of a MACS Separator to separate labeled from non-labeled cells ...
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bioRxiv - Cancer Biology 2024Quote: ... and FITC anti-human CD3 (1:50; REA613 clone; Miltenyi Biotec) antibodies.
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bioRxiv - Cancer Biology 2024Quote: ... T-ALL cells were isolated from the spleen and bone marrow of mice by negative selection with mouse CD45 microbeads (Miltenyi Biotec #130-052-301).
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bioRxiv - Cancer Biology 2024Quote: ... BM from CD45.1+ mice for hemogenic support were subjected to CD3 depletion (Miltenyi Biotec #130-094-973) and cells were harvested and washed with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Bone marrow (BM) from CD45.2 mice was subjected to Lineage depletion (Miltenyi Biotec #130-110-470). CD45.2 BM cells were plated at 1×106 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (Thermo Fisher #10639011 ...
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bioRxiv - Cancer Biology 2024Quote: Flow cytometry was performed on MACSQuant Analyzer 10 (Miltenyi Biotec, Bergisch Gladbach, Germany), and analysis of data was performed using FlowJo 10 (FlowJo LLC ...
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bioRxiv - Cancer Biology 2024Quote: Tissues samples were homogenized and lysed using gentleMACS Dissociators (Catalog No. 130-093-235, Miltenyi Biotec) with RIPA buffer ...
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bioRxiv - Cancer Biology 2024Quote: Protein extraction and digestion procedures were as follows: Tissues were homogenized and lysed using gentleMACS Dissociators (Catalog No. 130-093-235, Miltenyi Biotec, Nordrhein-Westfalen, Germany) with RIPA buffer (Catalog No ...
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bioRxiv - Microbiology 2024Quote: CD19+ B cells were purified by positive magnetic separation using CD19 MicroBeads (Miltenyi Biotec, Gaithersburg, MD) and MS or LS columns ...
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bioRxiv - Molecular Biology 2024Quote: ... and LNGFR MicroBeads (130-091-330, Miltenyi Biotec). Non transfected cells were taken along as a negative control ...
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bioRxiv - Immunology 2024Quote: ZFP36L2CKO and WT OT-I T cells were purified with the MACS CD8a+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Cd11b+ (Mac-1+) cells were isolated from unfractionated bone marrow using Cd11b MicroBeads (Miltenyi Biotec) following the manufacturers’ instructions.
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bioRxiv - Molecular Biology 2024Quote: ... and anti-CD8-APC (Miltenyi Biotec). Stained cells were pelleted (500 g ...
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bioRxiv - Molecular Biology 2024Quote: ... and FlowLogic™ software (Miltenyi Biotec).
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bioRxiv - Molecular Biology 2024Quote: ... Schizonts were isolated for imaging using magnetic purification (102) with a magnetic cell fraction column (MACS, Miltenyi Biotec) and a MidiMACS separator ...
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bioRxiv - Cell Biology 2024Quote: ... + 0.5x GlutaMax (Thermo 35050061) + 1x NEAA (Thermo 11140050) + 1x N-2 (Thermo 17502048) + 1x MACS NeuroBrew-21 (Miltenyi Biotec 130-093-566). On D3 ...
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bioRxiv - Cell Biology 2024Quote: ... stem cells were cultured on Matrigel (Corning 354230)-coated plates in StemMACS iPS-Brew XF (Miltenyi Biotec 130-104-368). The cell culture media was changed every other day and cells were passaged at a split ratio between 1 ...
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bioRxiv - Cell Biology 2024Quote: ... NM + 10 ng/mL GDNF (Miltenyi Biotec 130-129-547) + 10 ng/mL BDNF (Miltenyi Biotec 130-093-811 ...
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bioRxiv - Cell Biology 2024Quote: ... NM + 1 μM RA + 1 μΜ Purmorphamine (Miltenyi Biotec 130-104-465), was added and changed every other day until D17 ...
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bioRxiv - Cell Biology 2024Quote: ... + 4.25 μM CHIR99021 (Miltenyi Biotec 130106539), where NM is a mixture of 50% [v/v] DMEM:F12 + 50% [v/v] MACS Neuro Media (Miltenyi Biotec 130-093-570 ...
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bioRxiv - Cell Biology 2024Quote: ... where NM is a mixture of 50% [v/v] DMEM:F12 + 50% [v/v] MACS Neuro Media (Miltenyi Biotec 130-093-570) + 0.5x GlutaMax (Thermo 35050061 ...
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bioRxiv - Cell Biology 2024Quote: ... + 10 ng/mL BDNF (Miltenyi Biotec 130-093-811) + 200 μM L-ascorbic acid (Sigma A5960) ...
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bioRxiv - Cell Biology 2024Quote: ... Whole lungs were mechanically dissociated in GentleMACS C tubes (Miltenyi Biotec) containing 5 ml of PRMI 1640 (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Primary resting mouse splenic B-cells were isolated using anti-CD43 microbeads (Miltenyi Biotec) and activated with 25 ug/mL LPS (Sigma) ...
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bioRxiv - Bioengineering 2024Quote: ... Single-cell suspensions were prepared using Tumor Dissociation Kits (Miltenyi Biotec), filtered twice through 40 µm filters to remove clumps ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then labeled with Live/Dead dye and analyzed by MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec, San Diego, CA).
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bioRxiv - Cell Biology 2024Quote: ... Apoptotic cells (AV+ / PI-) were gated and counted using MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec).
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bioRxiv - Bioengineering 2024Quote: ... HSPCs were purified by immunomagnetic selection after immunostaining using the CD34 MicroBead Kit (Miltenyi Biotec). HSPCs were thawed and cultured at a concentration of 5×105 cells/ml in the “HSPC medium” containing StemSpan (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... reactions were labeled with 0.6μl anti-c-myc-FITC (Miltenyi Biotec), 0.25μl SAPE (streptavidin-R-phycoerythrin ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed three times with selection buffer and labeled with 10 μL streptavidin MACS microbeads (Miltenyi Biotec, 130-048-102) (sufficient for 1e7 cells) ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed three times with selection buffer and purified on LS-columns (Miltenyi Biotec) according to the manufacturer’s instructions for positive and negative selections.
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... human CD3+ positive selection kit (# 130-050-101), and murine CD3+ and CD8+ negative selection kits (# 130-095-130, # 130-104-075) were purchased from Miltenyi Biotec. SiR-Actin (# CY-SC001 ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... CD3+ T-cells were isolated using negative selection according to the manufacturer’s protocol (Miltenyi Biotec, # 130-095-130). T-cells were then resuspended in RPMI + 10% FBS and 200,000 cells were seeded per tension probe surface ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... OT-1 T-cells were magnetically isolated from spleens using CD8a+ negative selection according to the manufacturer’s protocol (Miltenyi Biotec, # 130-104-075). Purified OT-1 T-cells were resuspended in UCM ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... (Miltenyi Biotec, # 130-095-130). CD3+ T-cells were resuspended in UCM ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... CD4+ and CD8+ T-cells were isolated from spleens using CD4+ or CD8+ positive selection according to the manufacturer’s protocol (Miltenyi Biotec, # 130-117-043, 130-117-044). T-cells were resuspended in UCM ...
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bioRxiv - Bioengineering 2024Quote: ... Sorted cells were recovered in Complete NK MACS medium (Miltenyi Biotec, Gaithersburg, MD) with 100 IU/mL IL-2 and 2 ng/mL IL-15 for four days prior to cytotoxicity assays ...
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bioRxiv - Bioengineering 2024Quote: ... together with anti-c-myc fluorescein isothiocyanate (FITC, Miltenyi Biotec). After incubation for ∼1 h at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... T cell isolation was performed on the CliniMACS system using anti-CD4 and CD8 magnetic beads (Miltenyi Biotec, San Jose, CA). Next ...
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bioRxiv - Cell Biology 2024Quote: ... brain dissociation kits (Miltenyi Biotec 130-096-427, 130-107-677, 130-092-628), and gentleMACS C Tubes (Miltenyi Biotech 130-093-237) ...