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Citations for Becton, Dickinson and Company :
3651 - 3700 of 4284 citations for Nuclear pore complex protein Nup93 dye Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: The primary antibody cocktail was prepared in staining media together with a known number of APC Calibrite beads (BD Biosciences) and placed on ice ...
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bioRxiv - Cell Biology 2024Quote: ... BrdU-labeled DNA from each S-phase fraction (S1–S4) was immunoprecipitated using 0.5 μg of anti-BrdU antibody (BD #555627) and 20 μg of anti-mouse IgG (Sigma #M7023 ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 hour and probed overnight at 4°C with the following primary antibodies diluted in 5% non-fat dry milk/TBS-T: mouse monoclonal anti-p150 clone 1/p150Glued (BD Transduction Laboratories 610473 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with the appropriate antibodies described above to sort for live Ter119(-)CD45(+) or Ter119(-)CD45(-) cells using a FACSAria II (BD), which were used as input for single-cell sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were incubated with Fc receptor blocking antibody (CD16/CD32 Rat anti-Mouse, Unlabeled, Clone: 2.4G2, BD, fisher scientific) in washing solution (WS;1xPBS+1%FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were incubated with Fc receptor blocking antibody (CD16/CD32 Rat anti-Mouse, Unlabeled, Clone: 2.4G2, BD, fisher scientific) in washing solution (WS;1xPBS+1%FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Apoptosis in cell lines was assessed by the presence of cleaved poly (ADP-ribose) polymerase-1 (PARP) using a PE conjugated mouse anti-cleaved PARP (Asp214) monoclonal antibody (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were then spun down and resuspended in a mixture of the extracellular antibodies in brilliant stain buffer (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... twice and incubated with AF488-conjugated goat anti-mouse antibody (Invitrogen, A11001; 1:100 in blocking buffer (BD Bioscience, 347580)) before 15-minutes incubation with the FxCycle PI/RNase staining solution (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained in 0.1% BSA/PBS, containing following antibodies: EpCAM-APC (AB_1659714, eBioscience, 1:500) CD31 PeCy7 (AB_10612003, BD biosciences, 1:200), CD45 PeCy7 (AB_394489 ...
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bioRxiv - Biophysics 2020Quote: ... After incubation cell free supernatant was collected and subjected to sandwich ELISA using IL-2 specific antibody (BD biosciences, CA, USA) as per manufacturer instruction.
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed with sort buffer and incubated with cell surface antibodies mix diluted in the BV stain buffer (BD Biosciences) following manufacturer instructions for 30 minutes on ice in the dark ...
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bioRxiv - Cancer Biology 2021Quote: ... AKP-Z tumor derived cells were stained using the following antibodies: rat anti-mouse CD45-BV421 (clone 30-F11, 563890, BD Biosciences), rat anti-mouse CD31-BV421 (clone 390 ...
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bioRxiv - Microbiology 2020Quote: ... cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Pacific Blue; BD Biosciences), anti-CD4 (L200 ...
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bioRxiv - Immunology 2021Quote: ... were cultured with 5E5 of YAC-1 target cells (2:1 ratio) in the presence of anti-mouse CD107a-BV421 antibody (564347, BD Biosciences) (1:100 ...
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bioRxiv - Immunology 2021Quote: ... 1×105 Tconv cells were co-cultured with Treg cells at different ratios in the presence of 2×105 irradiated APCs and 1 µg/ml soluble anti-CD3 antibody (554829, BD Biosciences). The cells were cultured in T-cell complete media which consists of RPMI 1640 medium supplemented with 50 U/ml penicillin ...
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bioRxiv - Immunology 2021Quote: ... One million cells per sample were pre-incubated with purified anti-mouse CD16/CD32 antibody (Fc Block™, 553141, BD Biosciences). Live/dead cells were discriminated by staining cells with the LIVE/DEAD® Fixable Near-IR Dead Cell Stain kit (L10119 ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... a master mix containing a panel of fluorophore- or biotin-labeled antibodies was mixed at pre-titrated concentrations in Brilliant Violet Staining Buffer (BD Biosciences) at 2X ...
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bioRxiv - Immunology 2021Quote: ... for 5 minutes prior to staining at 4°C in the dark using the antibodies listed in supplementary table 1 and analysed using an LSR Fortessa cytometer (BD Biosciences) and FlowJo software (BD) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were incubated for 30 min in the dark at 4°C after adding relevant antibodies (BD biosciences, Franklin Lakes, NJ). A FACSCalibur system (BD biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... was prepared the day before the experiment by incubating the wells with 50μl/well of 5 μg/ml of anti-integrin α5 antibodies (BD Biosciences, 555651) in 0.05m Na2CO3 pH 9.6 ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight in blocking solution with the following primary antibodies (1:100): rat anti-mouse SiglecF (E50-2440, 552126 BD Biosciences), Armenian hamster anti-mouse CD80 (16-10A1 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated with pS6 or HK2 antibody for 1 h and O-GlcNAc antibody for 20 min at RT in the dark in cytoplasmic permeabilization buffer (BD Biosciences). Nuclear permeabilization buffer was used for c-Myc staining ...
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with intracellular cytokine staining antibodies for 20 min at room temperature in 1X perm/wash buffer (BD Biosciences). The details of antibodies are reported in the reporting summary ...
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bioRxiv - Immunology 2021Quote: ... prior to staining with a 2X master mix containing fluorophore-labeled antibodies at pre-titrated concentrations in Brilliant Violet Staining Buffer (BD Biosciences), and added to suspended cells at a final staining concentration of 2 x 108 cells/mL for 30-45 min ...
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bioRxiv - Immunology 2021Quote: ... prior to staining in the dark for 30 min on ice with fluorophore or biotin-labeled monoclonal antibodies prepared in brilliant stain buffer (BD Biosciences) ‘cocktail’ at pre-titrated quantities ...
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bioRxiv - Immunology 2021Quote: ... the brain sections were incubated with 3% serum for 1 hour followed by over-night incubation with primary antibodies (1:1000; Rat anti-mouse CD4 from BD Bioscience) in a humidified chamber at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Protein synthesis of Syndecan-1 was shown by immunocytochemistry using a polyclonal antibody against murine Syndecan-1 (CD138) clone 281-2 (1:50, rat anti-mouse, BD Pharmingen) in co-cultured cells after fixation with ice-cold 99% methanol and heparitinase III (2.4mIU/ml ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice and incubated overnight at 4°C with the fluorochrome-conjugated antibodies and unconjugated rat and mouse IgG in Perm/Wash solution (BD Biosciences), which was followed by additional 5 min incubation in Perm/Wash solution without antibodies ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed once with FACS buffer and then stained with surface antibodies diluted 1:200 in Brilliant buffer (BD Biosciences) for 30 minutes ...
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bioRxiv - Immunology 2022Quote: BMDMs were harvested as described before and Fc receptors were blocked with an anti-mouse CD16/CD32 antibody (1:250, BD Biosciences) in FACS buffer (5 % heat-inactivated FCS ...
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bioRxiv - Immunology 2022Quote: ... 1–3 ×106 PBMC were stained with a saturating concentration of the following antibodies: CD3 AF700 (clone SP34-2, BD Biosciences), CD4 eFluor450 (clone OKT4 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with PE-conjugated mouse anti-Human CD44 and mouse anti-Human CD184 antibodies (BD Biosciences, San Jose, CA) at a concentration of 5µl/10 million cells ...
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bioRxiv - Molecular Biology 2021Quote: ... and panned over prewashed 100-mm tissue culture plates coated with CD45 and CD16/32 antibodies (BD Biosciences, San Jose, CA). After incubation for 60 min at 37°C in a 5% CO2 atmosphere to promote adherence of contaminating macrophages and fibroblasts ...
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bioRxiv - Molecular Biology 2020Quote: ... or anti-Gr-1-PE (cells co-staining with anti-Mac-1 and anti-Gr-1 are deemed to be of the myeloid lineage) monoclonal antibodies (BD Pharmingen). The “percent repopulation” was determined based on the staining results of anti-CD45.2-FITC and anti-CD45.1-PE ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μl of platelet suspension was mixed with 40 μl of staining solution (HEPES-Tyrode buffer supplemented with 2.5 mM CaCl2, 1:50 PE rat anti-mouse CD41 antibody (BD Biosciences 561850), and 3:50 FITC Annexin V (BD Biosciences 556419)) ...
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bioRxiv - Cell Biology 2022Quote: ... were loaded onto SDS-PAGE gel and immunoblotted using antibodies against clathrin heavy chain (1:1,000; BD Bioscience, San Jose, CA), dynamin (1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... After washing, cells were sorted by fluorescence (antibody staining, Kaede-Green and Kaede-Red) on a BD FACS Aria (BD Biosciences) using FACS Diva software and analysis was performed using FlowJo (version 10 ...
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bioRxiv - Immunology 2022Quote: ... Naive CD4+ T cells were stimulated with plate-bound anti-CD3 (LOT: 4310627, eBioscience) and 1.5 μg/ml soluble CD28 monoclonal antibodies (Cat: 557393, BD biosciences pharmingen) in DMEM medium and cytokines (IL-6 ...
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bioRxiv - Immunology 2022Quote: The following mouse anti-human antibodies were used for purification of CD8 T cell subsets by Flow Cytometry: anti-CD45RO PE CF594 (clone UCHL1, BD Biosciences), anti-CD56 PE Cy7 (clone B159 ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cells were either stained with antibody dissolved in PBS containing 3 % albumin (surface markers) or permeabilized with Perm/Wash buffer (BD Biosciences) according to manufacturer’s instruction and stained with the respective antibody (mix ...
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bioRxiv - Immunology 2021Quote: ... TNFα and CD107a expression by multicolor flow cytometry panel (all antibodies used are listed in Table 1) in a BD LSR Fortessa Instrument (BD Biosciences). Polyfunctionality was evaluated using Boolean gating obtained with FlowJo v10 software.
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with fluorochrome-conjugated (FITC, PE, PE-Cy7, allophycocyanin [APC], APC-eFluor 780,) antibodies against mouse CD45 (30-F11; BD Biosciences), Ly6C (AL-21 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with sort buffer and incubated with cell surface antibodies that had been diluted in the BV stain buffer (BD Biosciences) for 30 minutes on ice ...
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bioRxiv - Immunology 2020Quote: Cells were stained with indicated fluorophore-conjugated antibodies and samples were collected on either Guava easyCyte HT Flow Cytometer (Luminex) or BD-Accuri C6 Flow Cytometer (BD Biosciences). For intracellular staining of Nur77 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4℃ after 1h blocking in 5% skimmed milk (Becton, Dickinson and Company, USA) at room temperature ...
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bioRxiv - Immunology 2020Quote: ... cover slips were washed 3 times in 1xPBS and incubated with secondary antibodies (anti-rabbit-AF647, anti-mouse-AF488 (BD Bioscience)) diluted 1:1000 in antibody diluent for 1 hour at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were fixed in 4% paraformaldehyde for 30 minutes and stained with PE-conjugated or purified CD45 antibody (eBioscience, 30-F11 or BD Biosciences) to ascertain the generation of haematopoietic progeny ...
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bioRxiv - Genomics 2021Quote: ... tissues were blocked (3% bovine serum albumin in 5% normal goat sera and 0.1% Triton) and incubated overnight at 4°C with primary antibodies: CD31 (Neomarkers, RB-10333-P0) or CDH1 (BD Biosciences, 6315829). Slides were washed and incubated with Cy3-goat-anti-mouse or anti-rabbit-conjugated secondary antibodies (Jackson Immunoresearch Laboratories ...
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bioRxiv - Immunology 2021Quote: Post-culture cells were pelleted in V-bottom 96-well plates and resuspended in 30 μl of antibodies at pre-optimized concentrations and diluted in Brilliant Stain Buffer (BD Biosciences), then incubated for 15’ at RT ...