Labshake search
Citations for Becton, Dickinson and Company :
2951 - 2978 of 2978 citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... either single GFP+ cells (to obtain clones 1 and 2) or 250 GFP+ cells (to obtain the pool) were sorted per well into a 96 well plate (BD FACSAria Fusion Cell Sorter). The two clones and the pool were expanded and the decreased ArgBP2 expression was confirmed by immunoblotting.
-
bioRxiv - Molecular Biology 2020Quote: ... cells were fixed using 2% paraformaldehyde (PFA) solution for 30 minutes and permeabilization was performed using a 1X BD perm/wash solution (BD Biosciences, USA; cat#554723) for 15 minutes at 4°C ...
-
bioRxiv - Immunology 2020Quote: ... the cells were co-cultured in R10 medium alone or in presence of K562 cells for 2 hours in the presence of anti-human CD107a (FITC or Brilliant Violet 421, H4A3, BD Biosciences, San Jose, Calif.).
-
bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs were assessed to determine the frequency of circulating T follicular helper (Tfh) using a panel which included the following antibodies: CD3 (BD Biosciences; clone SP34-2), CD4 (BD Biosciences ...
-
bioRxiv - Immunology 2020Quote: ... Cells were collected from wells by pipetting with PBS (containing 2%FBS and 2mM EDTA) and data acquisition was performed on a BD Accuri C6 (Becton, Dickinson and Company, USA). All flow cytometry data were analyzed with FlowJo v10.1 (BD Biosciences).
-
bioRxiv - Neuroscience 2022Quote: ... approximately 1-2 mm apart along the same bundle of muscle fibers with the help of a 20G syringe tip (BD Microlance, Thermo Fisher Scientific). After the dental cement hardened ...
-
bioRxiv - Bioengineering 2023Quote: ... Fixed and permeabilized cells were washed twice with PBSA buffer and incubated for 2 hr at room temperature with Alexa Fluor 647-conjugated anti-STAT5 pY694 antibody (BD Biosciences, clone 47/Stat5) diluted 1:50 in PBSA buffer ...
-
bioRxiv - Immunology 2024Quote: ... blocked with 2% BSA in phosphate buffer pH=7.4 for 30min at RT and incubated with mouse anti-human LAMP1 (BD Biosciences, Franklin Lakes, NJ, USA) for 1h at RT ...
-
bioRxiv - Microbiology 2024Quote: ... Bacterial strains were prepared for agar overlay plating by streaking out from glycerol stocks onto 2% streak agar plates (BD Difco 2216 marine broth), and allowed to grow for one days at room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were enumerated using a Cellometer X2 cell counter (Nexcelom Bioscience, Lawrence, MA, USA) and resuspended in yeast nitrogen base supplemented with 2% glucose (Difco; BD, Franklin Lakes, NJ, USA), to obtain a concentration of 2.0 × 106 cells/ml ...
-
bioRxiv - Neuroscience 2020Quote: Neurons from 18-day-old rat embryos were dissected and dissociated using trypsin and plated at a density of 70,000 cells cm−2 in minimal essential medium (MEM) supplemented with 10% NU serum (BD Biosciences, Le Pont de Claix, France), 0.45% glucose ...
-
bioRxiv - Neuroscience 2020Quote: ... for 1 h at room temperature followed by overnight incubation at 37°C in antibodies to the following: (1) C-terminal binding protein 2 (mouse anti-CtBP2; BD Biosciences, used at 1:200). (2 ...
-
bioRxiv - Immunology 2021Quote: ... and intracellular cytokines (PE anti-mouse IFN-γ, PE-Cy®7 anti-mouse TNF-α, APC anti-mouse IL-2, all from BD Biosciences, USA). The labeled cells were acquired with the BD FACSCanto™ II flow cytometer and analyzed with the program FlowJo v.10.6.2 (BD Biosciences).
-
bioRxiv - Microbiology 2021Quote: The actinobacteria were routinely cultured from frozen spore preparations via inoculation into Difco International Streptomyces Project (ISP) medium 2 (ISP2) (Becton, Dickinson and Company, Spark, MD) and incubated at 28° C ...
-
bioRxiv - Immunology 2023Quote: ... 5.0 × 104 CD4+ T cells were serially diluted onto either α-IL-2 or α-IFNɣ sterile coated PVDF 96 well plates (BD Biosciences, NJ, USA). The CD4+ T cells were then activated for 24 or 72 h in complete DMEM ...
-
bioRxiv - Molecular Biology 2023Quote: ... PMVECs were purified after two rounds of immune selection using magnetic beads coated with anti-CD31 and anti-ICAM-2 antibodies (BD Bioscience, Franklin Lakes, NJ, USA). mPMVECs were cultured and maintained in a medium containing 5 % FBS and EC growth supplement (ECGS ...
-
bioRxiv - Cancer Biology 2023Quote: ... BD 560046. After washing twice, cells were fixed in PFA 2% for 20 min, washed, and acquired on a Fortessa BD LSRII Cell Analyzer (Becton Dickinson).
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were washed with cold PBS/10% FBS and resuspended in 10 μl of cold PBS/10% FBS per 106 cells and stained with a 1/100 dilution of anti-Ter119-PE antibody (BD Pharmingen 553673 2 μg/ml) at 4 °C for 20 minutes ...
-
bioRxiv - Genetics 2022Quote: ... The proportion of HbF-and HbS-positive RBCs were measured with an antibody recognizing HbF (Thermo Fisher for HUDEP-2 cells or BD Biosciences for primary cells) or HbS (BioMedomics) ...
-
bioRxiv - Neuroscience 2024Quote: ... The nuclei were then stained with 4’,6-diamidino-2-phenylindole (DAPI) and flowed through a fluorescence activated cell sorter (FACS) (BD FACSCanto, BD Biosciences, Franklin Lakes, NJ, USA) to assess viability ...
-
bioRxiv - Immunology 2024Quote: ... membrane staining was conducted for 30 min at 4°C using the following antibodies: CD8 BUV496 (2:100; BD Biosciences; clone RPA-T8; Cat# 612942), CD4 Brilliant Violet (BV ...
-
bioRxiv - Cancer Biology 2021Quote: ... Clones were picked after 2–3 months of culture and expanded in the same medium on dishes coated with Collagen I (BD Biosciences, Le Pont de Claix, France) in humidified atmosphere with 5% CO2 at 37°C.
-
bioRxiv - Physiology 2021Quote: ... cells were washed twice with FACS buffer (PBS plus 2% heat inactivated fetal bovine serum) and analyzed on a SORP 8 Laser BD LSR flow cytometer (Becton, Dickinson and Company, Franklin Lakes, NJ).
-
bioRxiv - Immunology 2022Quote: ... cells counted under the microscope and stained in FACS buffer (PBS, 2% FCS, 2mM EDTA) with purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Catalog #553141) and the specific antibody cocktail ...
-
bioRxiv - Biophysics 2024Quote: ... transferred in a V-bottom 96-well plates and co-cultured with K562 cells (ATCC) at 1:1 effector-to-target ratio in the presence of mouse anti-human CD107a Alexa Fluor 647 (BD Bioscience, clone H4A3, 2 uL/well) for 6 hours at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... Non-specific antibody staining was inhibited using blocking buffer (male 10% human AB serum, 1% bovine serum albumin, 2 mM EDTA, 25 μg BD Fc Block; 15 min, 4°C) and washed before completing surface stain in staining buffer (PBS supplemented with 1% FBS ...
-
bioRxiv - Immunology 2023Quote: ... and media was tested by ELISA according to the manufacturer’s instructions (BD OptEIA Human IL-10 ELISA set #3555157 and BD OptEIA Human IL-1B ELISA set 2 #557953).
-
bioRxiv - Neuroscience 2023Quote: ... This was followed by staining for 30 min at 4°C with the appropriate antibodies: PE-Cy7 rat anti-mouse CD45 (cat # 561868, BD Biosciences, clone 30-F11, 2 ng/μl), BV421 rat anti-mouse CD11b (cat # 562605 ...