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Citations for Becton, Dickinson and Company :
2801 - 2850 of 2978 citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Mononuclear cells were washed three times with PBS and CD34+ cells were isolated as described 35 using rhesus macaque-specific antibodies to CD34 (clone 563; BD Pharmingen, San Jose, CA, USA) and the anti-PE MACS magnetic bead system (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2022Quote: ... detached by trypsin/EDTA and resuspended in complete cell culture medium for analysis on a BD LSR II Flow Cytometer (BD Biosciences, San Jose, CA, USA) connected to the BD FACSDiva™ software (BD Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... Disaggregated cells and organoids were resuspended in 150 - 200 µl of a 1:1 v/v mixture of cold DMEM/F12: Matrigel (BD Biosciences, San Jose, CA, USA). 8-12- week-old mice were anesthetized with isoflurane and the suspension was transplanted under the skin on the left flank using a 1 mL syringe and 21-gauge needle ...
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bioRxiv - Immunology 2020Quote: ... Single cells were stained with the different fluorescent antibodies (as indicated in the figure legends) and DsRed on the BD LSR II flow cytometer (BD Biosciences, San Jose, CA, USA). Analyses were completed using FlowJo analysis software (FlowJo ...
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bioRxiv - Cancer Biology 2020Quote: ... The viability of the cells was measured post 48 hours of drug treatment using annexin-V-7AAD viability kit (BD Pharmingen, San Diego, CA, USA) as per the manufacturer’s protocol.
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bioRxiv - Biophysics 2020Quote: ... All agar or broth cultures were maintained at 37°C under microaerobic conditions in a tri-gas environment containing 5 – 12% CO2 and 5 - 15% O2 using BD GasPak systems (BD Biosciences, San Jose, CA, USA) consisting of a non-vented cylinder or a heavy Ziplock bag ...
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bioRxiv - Systems Biology 2020Quote: ... The labeled cells were washed with cold PBS and the signals were detected using a BD FACSCanto II instrument (BD Biosciences, San Jose, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... TMF and C6-NBD-cer fluorescence intensities were acquired on the FL1-H channel (530/30 bandpass filter) while PI fluorescence on the FL3-H channel (670LP filter) of a FACSCalibur (BD Biosciences, San Jose, CA, USA). Post-analysis was performed in the software Summit (version 4.3 ...
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bioRxiv - Immunology 2020Quote: ... The binding of Cy3-xfR5 mAb and Cy3-xfR5-D+T NPs to respective T-cell type was detected and evaluated respectively by the BD LSRII flow cytometer instrument (BD Biosciences; San Jose, CA, USA) and Flowjo software v10 (BD ...
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bioRxiv - Cancer Biology 2021Quote: ... were collected from transwell assay and the mean fluorescence intensity of the green signal (VC3AI) was then determined by FACS Calibur flow cytometry (BD Biosciences, San Jose, CA, USA). Control cells were treated with 1 µM Actinomycin D as a positive control for cell death.
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bioRxiv - Cancer Biology 2021Quote: ... mice received suspension of 0.2 mg FPM in a total volume of 50 μl of sterile physiological saline by inserting a 7-gauge needle (BD Biosciences, San Jose, CA, USA) into the trachea trans-orally ...
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bioRxiv - Cancer Biology 2021Quote: ... Flow cytometry for analysis of cell proliferation and apoptosis was performed at the Siteman Cancer Center Flow Cytometry Core on a FACScan analyzer (BD Biosciences, San Diego CA, USA) using a standard propidium iodide staining protocol as described previously (48).
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bioRxiv - Immunology 2021Quote: ... cells were washed twice with 1× Permeabilization buffer and data was collected using a flow cytometer (LSRFortessa X-20, BD Biosciences, San Jose, CA, USA). Data were analyzed using Kaluza or FlowJo 10 ...
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bioRxiv - Microbiology 2021Quote: Phytoplankton groups were enumerated using a BD Influx flow cytometer equipped with a forward scatter (FSC) detector with small particle option (BD Biosciences, San Jose, CA, USA). Prochlorococcus ...
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bioRxiv - Immunology 2020Quote: ... then cell sediments were suspended in 1% paraformaldehyde buffer (300ul) and acquired on a on a Fluorescence Activated Cell Sorter (FACS) Calibur flow cytometer (BD Biosciences, San Jose, CA, USA). All samples acquired in this study were compensated using the single-color stained cells ...
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bioRxiv - Immunology 2021Quote: ... IgA and IgM antibodies were quantified in the CVL specimens using the human immunoglobulin cytokine bead array (CBA) flex set assay (BD Biosciences, San Jose, CA, USA). The Ig CBA assay was performed as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... mCherry was co-expressed with FXa for FACS-sorting to purify transduced cells using a FACS Aria II cell sorter (BD Biosciences, San Jose, CA, USA). Conditional supernatants from lentivirus-infected CHO cells sorted by FACS were used to purify the FXa-Fc fusion protein using a protein G column (89927 ...
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bioRxiv - Immunology 2020Quote: ... Jurkat cells were maintained in culture for 1 week using enhanced RPMI and screened for TCR expression (Jing et al., 2016) using an anti-mouse TCR β chain (mTCRBC) APC antibody (BD Biosciences, San Jose, CA) and tetramer staining as described above.
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bioRxiv - Genomics 2021Quote: ... cells in at least two wells of a 6-well culture dish were released from the dish using Accutase (BD Biosciences, San Jose, CA, #561527). Samples were washed three times and resuspended in 1X PBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... approximately one million G1 and S phase cells for macaque LCL sample 76-06 and chimpanzee iPSC sample C3649 were sorted using a FACSAria Fusion (BD Biosciences, San Jose, CA, USA), DNA was extracted and sequenced as above ...
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bioRxiv - Developmental Biology 2022Quote: ... Flow analysis was run on a CytoFlex LX system (BeckmanCoulter, Brea, CA, USA) and population gating and tSNE analysis were performed with FlowJo (BD Biosciences, Franklin Lakes, NJ, USA). The gating strategy was adapted as previously published 90 ...
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bioRxiv - Microbiology 2022Quote: ... IL-6 and IL-10 levels in cell culture supernatants were determined using the OptEIA™ mouse IL-10 ELISA kit (BD Biosciences, San Jose, CA) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in a binding buffer containing 1 μg/mL propidium iodide (PI) and 1 μg/mL APC-labeled annexin V (BD Pharmingen, San Diego, CA, USA). All specimens were analyzed by flow cytometry (FACSCalibur ...
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bioRxiv - Cell Biology 2024Quote: ... Samples of 30-50 mL peripheral blood were collected by venipuncture from 68 individuals with T1D in BD Vacutainer Sodium Heparin tubes (BD Biosciences, San Jose, CA, USA). Inclusion criteria were ...
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bioRxiv - Genetics 2024Quote: Differentiated erythrocytes were analyzed by flow cytometry on day 14 for erythrocyte lineage-specific markers using a FACS Aria II (BD Biosciences, San Jose, CA, USA). Edited and unedited cells were analyzed using the following antibodies ...
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bioRxiv - Microbiology 2023Quote: ... purified gametocytes from DFsc7 or DFsc7;ΔRbpm1 lines were suspended in GMB at 4°C and sorted using a BD FACS AriaIII instrument (BD Biosciences, San Jose, CA, USA) based on their GFP and mCherry fluorescence intensity for male and female gametocytes ...
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bioRxiv - Cancer Biology 2024Quote: ... single GFP-expressing cells were sorted in 96-well plates by a fluorescence-activated cell sorter (FACS) FACSAria II cell sorter (BD Biosciences, San Jose, CA, USA). Two weeks later ...
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bioRxiv - Genomics 2023Quote: ... 50 µl of SYBR Green I stained RBCs were analyzed on BD LSR Fortessa Flow Cytometer with High Throughput sampler (BD Biosciences, San Jose, CA, USA) using BD FACS Diva Software v6.2 and 488 laser excitation / 530 emission filter to determine the concentration of SYBR Green I positive cells per microliter ...
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bioRxiv - Cell Biology 2023Quote: ... Stained cells were diluted 4x in 1x Annexin V Binding Buffer and analysed by flow cytometry performed using the BD FACSCanto II (Becton Dickinnson and Company, BD Biosciences, San Jose, CA, USA). Data was analysed using FlowJoTM Software ...
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bioRxiv - Biochemistry 2023Quote: ... and mCherry in response to HSLs were analyzed at a low flow rate in a FACS™Aria III system (BD Biosciences, San Jose, CA) using 488 nm ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting cell pellets were resuspended in 500 μL of PBS or DMEM (-FBS) and filtered using a 35-μm cell strainer (BD Biosciences, San Jose, CA, USA) into single-cell suspensions for FACS analysis.
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bioRxiv - Immunology 2023Quote: The cell surface quantification of GD2 on tumor lines was enumerated by flow cytometry using BD QuantiBRITE beads and Custom Quantitation Beads (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice in PBS and incubated with 25 µl of an anti-mouse CD16/CD32-Fc/Block (BD Bioscience, San Jose, CA, USA) 20 minutes on ice in the dark ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... NP-FB23-2 (3 mg/kg) was delivered through the retro-orbital sinus into neonatal mice with a 31-gauge insulin syringe (BD Biosciences, San Jose, CA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... The final pellet containing organoids was re-suspended in Matrigel and distributed in 96-well culture plates containing an underlay Matrigel layer (Growth Factor Reduced Matrigel, BD Biosciences, San Jose, CA, USA). Cultures were maintained at 37 °C in serum-free basal medium (DMEF/F12 ...
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bioRxiv - Cancer Biology 2023Quote: Cell cycle analysis of cells cultured in liquid medium was performed as described 26 using a FACS Canto II flow cytometer (BD Bioscienes, San Jose, CA, USA). For cell cycle analysis of cells embedded in soft agar ...
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bioRxiv - Bioengineering 2023Quote: ... cells were lysed and fixed in BD Phosflow Lyse/Fix Buffer and then were permeabilized with BD Phosflow Perm Buffer III (BD Biosciences, San Jose, CA, USA) following manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated with fluorochrome-labeled monoclonal antibodies after blocking the Fc receptor with anti-CD16/32 monoclonal antibodies (BD Pharmingen, San Diego, CA. USA). Data were analyzed with FlowJo or Kaluza software ...
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bioRxiv - Bioengineering 2023Quote: ... mutans UA 159 strains were maintained and incubated on solid TSB (Tryptic Soy Broth) or BHI (Brain Heart Infusion; BD Bioscience, San Jose, CA, USA) agar plates for 24 h at 37°C and 5% CO2 ...
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bioRxiv - Genomics 2023Quote: ... The cardiac Troponin T (TNNT2) antibody (Cardiac Troponin T Mouse, PE, Clone: 13-11, BD Mouse Monoclonal Antibody (564767, BD Biosciences, San Jose, CA, USA) was diluted in Permeabilization Buffer (FOXP3/Transcription Factor Staining Buffer Set ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 × 106 cell/mL cells were stained with the appropriate antibodies and analyzed by flow cytometry (BD FACS Fortessa, BD Biosciences, San Jose, CA, USA). Fluorescence minus one (FMOs ...
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bioRxiv - Cell Biology 2024Quote: FACS analysis and cell sorting of Ai9+ cells from primary dermal explants were performed using a BD FACSAria II (BD Biosciences, San Jose, CA, USA). Primary cell culture pellets were resuspended into FACS Staining Media (HBSS supplemented with 10% FBS ...
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bioRxiv - Microbiology 2024Quote: ... and the cultures were mixed vigorously with 100 µL of 10% Casamino Acids technical grade (CA-T; Becton, Dickinson and Company, Franklin Lakes, NJ, USA) to detach the bacterial cells from the support (28) ...
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bioRxiv - Molecular Biology 2024Quote: ... Whole blood was sampled from the patients via the femoral arterial sheath during the mechanical thrombectomy and stored using a PAXgene RNA blood collection tube (762165, BD Biosciences, San Jose, CA, USA) in a -80°C freezer until the subsequent steps ...
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bioRxiv - Microbiology 2024Quote: ... The amount of coating gp120 was optimized through competition by binding of the Leu3A (anti-CD4) antibody (clone SK3; Catalog no. 340133; Final dilution 1:5; BD Bioscience, San Jose, CA, USA). Cryopreserved peripheral blood mononuclear cells ...
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bioRxiv - Immunology 2021Quote: ... and anti-inflammatory cytokines (IL-10, TGF-β) were determined in plasma by ELISA at the indicated time points (OptEIA set; BD Biosciences, San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... USA) and the lower half with a primary 42-kDa anti-β-actin antibody (chicken, ab-5, anti-mouse IgG, BD Biosciences, San Jose, CA, USA). Both antibodies were diluted 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... The base of the UC of the CIM plate was coated with 20% solution of a basement membrane matrix (BD Matrigel, BD Biosciences, San Jose, CA) in serum-free medium ...
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bioRxiv - Microbiology 2021Quote: ... All samples were then filtered through 60-µm nylon Blutex just before flow cytometric analyses were performed using a BD LSR Fortessa™X-20 (BD Biosciences, San Jose, CA, USA). BD FACSDiva™software (v 8.0.2 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then surface stained for 30 min in the dark at 4°C with viability reagent (BD Horizon™ Fixable Viability Stain, BD Biosciences, San Diego, CA, USA) and a 29-color cocktail of monoclonal antibodies (mAbs ...