Labshake search
Citations for Becton, Dickinson and Company :
2501 - 2550 of 2978 citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... followed by 30 min in blocking solution (PBS supplemented with FBS 2%, saponin 0.2%, NaAz 0.1%, 1:1000 FC blocker (BD Bioscience; 553142)) ...
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bioRxiv - Microbiology 2024Quote: ... were incubated on ice for 30 min in the dark in 50 ul of PBS containing 2 ul of APC-labeled mouse anti-human CD4 or anti-human CD184 (CXCR4) antibody (BD biosciences 561840 and 560936 ...
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bioRxiv - Immunology 2024Quote: ... and stored in FB 4C overnight before analysis on a 3 laser Aurora spectral flow cytometer (Cytek, figure 1D, Supplementary Figure 2) or X20 Special Order 5-lasers Fortessa (BD) flow cytometer (other figures) ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclei were then stained with 4’,6-diamidino-2-phenylindole (DAPI) and flowed through a fluorescence activated cell sorter (FACS) (BD FACSCanto ...
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bioRxiv - Immunology 2024Quote: ... We resuspended cells in FACSWash (1x PBS, 2% FBS, 0.2% sodium azide) and acquired events on a LSRFortessa (BD Biosciences), which we analyzed using FlowJo v10 (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... cells were suspended in PBS containing 0.5% FBS and 2 mM EDTA and were sorted with a 100-μm nozzle using BD FACSAria III (BD Biosciences). Sorted cells were pelleted and lysed in RNA lysis buffer (Zymo Research).
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... Nuclei were resuspended in 1-2 mL of staining buffer (depending upon the starting material and yield) and filtered into a FACS (BD) tube ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then washed twice with DPBS containing 2% BSA and 0.01% saponin before flow cytometry analysis using a BD FACSymphony A5 Cell Analyzer (BD Biosciences) at a fixed speed ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 37°C and dead cells were labeled using the LIVE/DEAD Fixable Aqua (405 nm) Dead Cell Stain Kit (BD Biosciences, San Jose, CA). EdU detection ...
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bioRxiv - Cancer Biology 2020Quote: ... Stable shRNA silencing cell clones were selected by relevant antibiotics followed by cell sorting by the FACSAria III cell sorter flow cytometer (BD Biosciences, San Jose, CA).
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bioRxiv - Neuroscience 2021Quote: ... Stimulated cells were stained with fluorochrome-conjugated anti-MCP-1 and anti-IL-6 Abs using the Cytofix/Cytoperm kit (BD Biosciences, San Jose, CA) to determine MCP-1 and IL-6 production ...
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bioRxiv - Neuroscience 2021Quote: ... FAC-sorting was performed at Brigham Young University (Provo, UT, USA) using a FACS Aria Fusion Cell Sorter (BD Biosciences, San Jose, CA, USA). Cells were sorted at a reduced flow rate immediately into methanol to preserve gene expression and pooled together for rehydration and resuspension in preparation for sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... The mice were transplanted with a two-step procedure: half the mixture of EGFP+ and mCherry+ transduced cells was solidified by matrigel (BD Bioscience, San Jose, CA), mixed with CD34-cells as feeder cells (4.5×106 cells) ...
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bioRxiv - Molecular Biology 2020Quote: ... The resuspended nuclei were filtered with 40 μm strainer and immediately underwent fluorescence activated cell sorting (FACS) using a BD FACSAria IIu (BD Biosciences, San Jose, CA) with 70 μm nozzle at the MSU Pharmacology and Toxicology Flow Cytometry Core (drugdiscovery.msu.edu/facilities/flow-cytometry-core) ...
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bioRxiv - Molecular Biology 2021Quote: ... stained with a collection of different fluorescent labelled antibodies (CD4-BV785; BioLegend, San Diego, CA, USA; CD25-PE-Cy5.5; BD Bioscience; and CD54-AF700; EXBIO) and analysed on a Cytoflex flow cytometer (Software CytExpert 2.3 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were harvested and then resuspended in phosphate-buffered saline (PBS) before being analyzed by a LSRFortessa X-20 flow cytometer (BD Biosciences San Jose, CA). The threshold for detection was set at 10,000 counting events ...
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bioRxiv - Neuroscience 2020Quote: EAE was induced in 7-week-old female C57BL/6J mice by subcutaneous flank administration of 200 μg MOG35-55 peptide emulsified with complete Freund’s adjuvant (CFA) (MOG35-55/CFA) (BD Biosciences, San Jose, CA, USA) supplemented with inactive ...
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bioRxiv - Immunology 2022Quote: ... The lungs and spleens were homogenized in saline and serial dilutions were plated on 7H11 agar plates supplemented with OADC (BD Biosciences, San Jose, CA). After 3-4 weeks of incubation at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Human immune cell activation in peripheral blood and lymphocytes isolated from the spleen were measured by a fluorescence-activated cell sorter (FACS) Aria II flow cytometer (BD Biosciences, San Jose, CA) using antibodies against mCD45-APC ...
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bioRxiv - Microbiology 2020Quote: ... human immune cell reconstitution in peripheral blood was measured by a fluorescence-activated cell sorter (FACS) Aria II flow cytometer (BD Biosciences, San Jose, CA) using antibodies against mCD45-APC ...
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bioRxiv - Immunology 2021Quote: ... SR01, were stained with the A0201-KLVALGINAV-PE tetramer (NIH tetramer Core Facility, Emory University) and CD8-FITC Ab (BD Biosciences, San Jose, CA), and sorted on a FACS Aria cell sorter ...
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bioRxiv - Cancer Biology 2020Quote: ... Enumeration of human CD45+ cells in spleen and bone marrow was performed by flow cytometry after staining with BV605-conjugated human-specific CD45 antibody (clone HI30, BD Biosciences, San Jose, CA) using a BD LSRII flow cytometer (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... 20μL cell suspension was passed into a new 96-well plate with 180μL fresh media and the ratio of cells expressing different fluorescent proteins was measured by flow cytometry using a BD FACSCanto II (BD Biosciences, San Jose, CA). For competition assays with dasatinib (Selleck Chemicals ...
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bioRxiv - Immunology 2021Quote: ... Single leucocyte suspensions were stimulated with either phorbol 12-myristate 13-acetate (PMA)-ionomycin or 5 µg/ml of MCMV peptide pool (Table S3) for 6 hours with brefeldin A (BD Biosciences, San Jose, CA). After stimulation ...
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bioRxiv - Genomics 2020Quote: ... mechanically disaggregated cells and clumps of cells were resuspended in 100-200µl of a 1:1 v/v mixture of cold DMEM/F12: Matrigel (BD Biosciences, San Jose, CA, USA). 8-12 weeks old mice were anesthetised with isoflurane ...
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bioRxiv - Neuroscience 2020Quote: ... All data were compensated and spectral overlap was minimized using the automatic compensation method of BD FACS Diva software (BD Biosciences, San Jose, CA).
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bioRxiv - Bioengineering 2020Quote: ... Cells were resuspended in PBS + 5% FBS and analyzed via Fluorescence-Assisted Cytometry (FACs) using a BD LSR Fortessa (BD Biosciences, San Jose, CA). Fluorescence minus one (FMO ...
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bioRxiv - Cancer Biology 2020Quote: ... Flow sorting for live and non-erythroid cells (DAPI-neg/CD235-neg) was performed on a BD FACS Aria III equipped with a 100um nozzle (BD Biosciences, San Jose, CA) instrument ...
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bioRxiv - Cell Biology 2021Quote: Cell cycle analysis was determined by flow cytometry (FACS) of propidium iodide (PI)- stained cells using BD FACS Calibur (BD Biosciences, San Jose, CA) as described earlier (Bacevic et al ...
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bioRxiv - Cell Biology 2021Quote: ... 200 µg/ml RNase A in PBS) for 15 min at room temperature and subjected to cell cycle analysis using BD FACS Calibur (BD Biosciences, San Jose, CA). Data were analysed using FlowJo software (v10.6.1 ...
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Suppressing chemoresistance in lung cancer via dynamic phenotypic switching and intermittent therapybioRxiv - Cancer Biology 2020Quote: ... with assistance from the Analytical Cytometry Core at City of Hope using the BD FACSAria II cell sorter (BD Biosciences, San Jose, CA, USA), the mixed population was separated based on the fluorescence tag ...
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bioRxiv - Cell Biology 2021Quote: ... An adult mouse retina cDNA library was cloned in pGADT7 (carrying a Trp1 selection gene) and transformed in AH109 yeast strain (containing His3, Ade2, lacZ and Mel1 selections; Clontech, BD Biosciences, Pao Alto, CA). Cpne4 vWA domain was cloned into pGBKT7 (carrying Leu2 selection gene ...
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bioRxiv - Systems Biology 2020Quote: ... HL60 and U937 cells (1 mL) were immediately fixed by addition of 0.2 mL of x5 Lyse/Fix buffer (BD Biosciences, San Jose, CA, USA) for 10 min at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... single cells were isolated and sorted into a 96 well plate using an Influx Special Order fluorescence activated cell sorter (BD Biosciences, San Jose, CA) at the Stanford Shared FACS Facility ...
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bioRxiv - Microbiology 2021Quote: Detection of extracellular nectin-1 in infected cells was performed using a BD LSR II or BD LSR Fortessa cytometer (BD Biosciences, San Jose, CA). Infected cells were harvested by 5mM EDTA supplemented with Dulbecco’s PBS (D-PBS ...
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bioRxiv - Physiology 2021Quote: ... islets were dispersed and dead cells were labeled using the LIVE/DEAD Fixable Aqua (405 nm) Dead Cell Stain Kit (BD Biosciences, San Jose, CA). EdU detection ...
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bioRxiv - Molecular Biology 2020Quote: ... The base of the UC of the CIM plate was coated with 20% solution of a basement membrane matrix (BD Matrigel, BD Biosciences, San Jose, CA) in serum-free medium ...
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bioRxiv - Molecular Biology 2021Quote: ... Stained peripheral blood samples were then lysed with red blood cell lysis buffer and absolute cell counts calculated using BD Liquid Counting Beads (BD Biosciences, San Jose, CA). Flow cytometry was performed using BD Fortessa II instrument (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... the organoids were removed from the Matrigel and broken up mechanically by passing them through a syringe and needle (27 G, BD Biosciences, San Jose, CA), and then ...
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bioRxiv - Cancer Biology 2020Quote: ... This approach has previously been validated to show differences in tumorigenic potential between CD133-positive and CD133-negative fractions.20–24 Organoids were formed as previously described25 by suspending tumor cells in 80% Matrigel (BD Biosciences, San Jose, CA) and forming 20 μl pearls on parafilm molds prior to culture ...
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bioRxiv - Cell Biology 2020Quote: ... GFP positive cells were collected in the Rockefeller University Flow Cytometry Resource Center using a FACSAria II flow cytometer (BD Biosciences, San Jose, CA). Cells were resuspended in PBS (no calcium/no magnesium ...
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bioRxiv - Microbiology 2022Quote: ... The cells were collected to detect virus-infected cells via flow cytometry by using a BD LSR II flow cytometer (BD Biosciences, San Jose, CA)) at 48-h post-infection [45] ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescence was measured at a fixed emission (670 nm) after excitation with a red laser (640 nm) using a BD FACSVerse Flow cytometer (BD Biosciences, San Jose, CA) with 10,000 events per sample ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescence was measured at a fixed emission (585 nm) after excitation with green-yellow laser (561 nm) using a BD FACSAria Flow cytometer (BD Biosciences, San Jose, CA). 10,000 events were acquired per sample.
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bioRxiv - Developmental Biology 2022Quote: ... Cells with CD41-GFPlow positive signal were subjected to fluorescence-activated cell sorting (FACS) with an influx cytometer (BD Biosciences, San Jose, CA, USA). Single-cell RNA sequencing was performed according to an adapted version of the SORT-seq (Muraro et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting cell suspension was passed through a standard syringe needle and subsequently through a 70-μm nylon filter (BD Biosciences, San Jose, CA). No established cell lines were used in this study.
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice with staining buffer and analyzed on a BD-LSRII flow cytometer using FACS Diva Software (BD Biosciences, San Jose, CA).
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bioRxiv - Cancer Biology 2022Quote: Cell viability was determined using a resazurin assay and cell death measured using the Annexin-V FITC apoptosis detection kit (BD Biosciences, San Jose, CA). Methods have been previously described (65 ...
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bioRxiv - Immunology 2020Quote: The following rat anti-mouse antibodies were used for leukocyte analysis within each tissue: purified CD16/CD32 Fc blocking antibody (clone 2.4G2; BD Pharmingen™, San Jose, CA), PerCP-Cy™5.5 Ly6G antibody (clone 1A8 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The intercellular exchange efficacy between donor and recipient cells was measured by flow cytometry using a BD FACS Calibur flow cytometer (BD Biosciences, San Jose, CA). The intercellular exchange efficacy was calculated as: where PAgNP-positive stands for percentage of AgNP-positive recipient cells and MAgNP-positive stands for mean fluorescence intensity in AgNP-positive recipient cells.