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Citations for Becton, Dickinson and Company :
2551 - 2600 of 2978 citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... surface stained, fixed with 4% formaldehyde solution (Tousimis, Rockville, MD) and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA). Antibodies used for flow cytometry included anti-human CD3 AF700 (clone UCHT1) ...
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bioRxiv - Neuroscience 2020Quote: ... The transfer of the retinal suspension onto panning plates bound with monoclonal mouse anti-SSEA-1 (CD15)(MC480 clone) (#BD560079; BD Pharmingen, San Jose, CA) and monoclonal mouse anti-HNK-1/N-CAM (CD57)(VC1.1 clone ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stained with Alexa Fluor 647–conjugated anti– human CD24 and APC-H7 labeled anti–human CD44 antibodies (BD Pharmingen, San Jose, CA) at 4°C for 20 min ...
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bioRxiv - Microbiology 2021Quote: ... For flow cytometry measurements (forward and side scatter as well as green fluorescence) an Accuri C6 flow cytometer (BD Biosciences, San Jose, CA, US) with the corresponding software ...
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bioRxiv - Molecular Biology 2022Quote: ... crosslinked lungs were cut into small pieces using a razor blade then dissociated into single cell suspensions using Medicon filters (BD Biosciences San Jose, CA), washed with cold 1X PBS plus 1 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Microbiology 2022Quote: ... Separated cells were then washed twice with phosphate buffered saline (PBS, pH 7.4) and filtered through a 40 μm cell strainer (BD Biosciences, San Jose, CA, USA). Tissue specimens were shredded with scissors and filtered using a 40 µm or 100 µm cell strainer (BD Biosciences ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... CD34+CD45RA-Lin(CD3, CD14, CD56, CD19)- eGFP+ HSC were sorted 48 hours after transduction using the FACSAria III cell sorter (BD Biosciences, San Jose, CA) and cultured on a monolayer of mitomycin C-inactivated (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 30 min at 4°C with a cocktail of mouse anti-human antibodies: CD19 Alexa 700 (HIB19, BD Biosciences, San Jose, CA), CD21 BV421 (B-ly4 ...
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bioRxiv - Immunology 2022Quote: ... cells were filtered through a 70 µm cell strainer into FACS tubes and analyzed using a BD LSRFortessa (BD Biosciences, San Jose, CA, USA). Single cells were gated and tdTomato fluorescence was quantified in the PE channel using FACS Diva and Flowjo software.
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bioRxiv - Genomics 2020Quote: ... at a concentration of 100μg/ml starting 5 days after transfection for the following 7-9 days before single cell sorted using BD FACSAria II (BD Bioscience, San Jose, CA) for clonal cell line generation ...
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bioRxiv - Genetics 2020Quote: ... Cells were stimulated at 1×106 cells/ml with 10 μg/mL soluble Leishmania antigen (SLA) plus 1 μg/mL purified NA/LE anti-human CD28 (clone CD28.2, BD Biosciences, San Jose, CA, USA) at 37°C / 5% CO2 for 15 h and subsequently incubated in the presence of brefeldin A (BD Biosciences ...
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bioRxiv - Pathology 2020Quote: ... detached and subsequently washed in 1× PBS and analyzed by flow cytometry with the phycoerythrin (PE) channel on 532 nm using a BD LSRII flow cytometer (BD Biosciences, San Jose, CA). The results are summarized (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... FACS was then used to separate single RFP-positive cells in a 96-well plate using the 561 nm laser on a FACSAriaIII (BD Biosciences, San Jose, CA). Indels at the corresponding target sites in the clones were analyzed by genomic PCR ...
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bioRxiv - Cancer Biology 2021Quote: ... or 4T1.2 cells (1⨯106 per fat pad) in 50 μL of 50:50 (v/v) PBS:Matrigel™ (BD Biosciences, San Jose, CA, USA). Tumor growth was measured twice weekly by caliper measurements and weekly bioluminescent imaging (125 mg/kg luciferin I.P. ...
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bioRxiv - Bioengineering 2021Quote: ... cells were stained with surface marker antibodies and analyzed with Fluorescence-Assisted Cytometry (FACs) using a BD LSR Fortessa (BD Biosciences, San Jose, CA). Propidium Iodide (5:1000 µL ...
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bioRxiv - Immunology 2020Quote: 10 ml of peripheral venous blood from each cured VL patients and healthy control volunteers (endemic) was collected in LPS-free conditions into Li-heparin vacutainers (BD Biosciences, SanJose, CA, USA). The PBMC was isolated within 10 min after blood collection by density gradient centrifugation over Ficoll-Paque Plus (GE Healthcare ...
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bioRxiv - Synthetic Biology 2020Quote: ... Experiments involving mCherry (Ub R-nE7-mCherry and Ub X-LaM4-EGFP rescued with mCherry) were conducted on BD FACSAria Fusion High Speed Cell Sorter (BD Biosciences, San Jose, CA). All flow cytometry experiments involved ≥50,000 transfected cells as determined by forward- and side-scatter profiles of recorded events and fluorescent gating to exclude cells not transfected by at least the rescuing protein for each respective experiment ...
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bioRxiv - Immunology 2020Quote: We labeled each group of NALT cells separately with the Sample-Tags from the BD Mouse Immune Single-Cell Multiplexing Kit (BD Biosciences, San Jose, CA), described in “Single Cell Labeling with the BD Single-Cell Multiplexing Kits” protocol ...
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bioRxiv - Immunology 2020Quote: We uploaded and analyzed the FASTQ files on Seven Bridges website (Seven Bridges Genomics) by running the “BD Rhapsody WTA Analysis Pipeline” (BD Biosciences, San Jose, CA). After performing alignment ...
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bioRxiv - Biochemistry 2020Quote: ... and TNF in the supernatants was determined by using Cytometric Bead Array (CBA) - Human Inflammatory Cytokines Kit (551811, BD Biosciences, San Jose, CA, USA), that allows the determination of the indicated human cytokines simultaneously ...
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bioRxiv - Immunology 2021Quote: ... Assay plates were incubated 24-48 h at 37⍰°C in a 5% CO2 incubator and developed using BD ELISpot AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISpot reader and ImmunoSpot®software v6 (Cellular Technology ...
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bioRxiv - Cell Biology 2021Quote: Flow cytometry was performed at the Gladstone Institutes Flow Cytometry Core Facility using two instruments: an LSR II (BD Biosciences, San Diego, CA, US) and FACSAria II (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Spleen cells were isolated by scraping with slides in sterile PBS following red blood cell (RBC) lysis (Pharm LyseTM; BD Bioscience, San Jose, CA). Extracted LN were derived from the cervical and axillary chains exclusively.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Whole blood was collected from human volunteer donors with informed consent in 8 to 10 BD Vacutainer CPT 8 ml tubes (BD Biosciences, San Jose, CA). The blood samples were mixed immediately prior to centrifugation by gently inverting tubes 8-10 times ...
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bioRxiv - Cell Biology 2020Quote: ... re-suspended in αMEM (with 10% FBS and 1% anti-mycotic) and then processed on an Aria II or Influx flow cytometer (BD Biosciences, San Jose, CA) to separate tdTomato-expressing (tdTom+ ...
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bioRxiv - Immunology 2021Quote: ... The stimulation was performed by incubation for 6 h at 37°C and 5% CO2 in the presence of Protein Transport Inhibitor Cocktail (brefeldin A) (BD Biosciences, San Jose, CA) at a final concentration of 1:1000 ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for 30 min at room temperature in the staining solution before being subjected to flow cytometry analysis (FACSCalibur, BD Biosciences, San Jose, CA).
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bioRxiv - Immunology 2021Quote: ... CD8+ and CD4+ T cells from the tumor and CD8+ CD44hi and CD4+ CD44hi T cells from the dLN were sorted using FACS Aria II or Aria III (BD Biosciences, San Jose, CA). Nonviable cells were excluded from the sorting gate based on forward and side scatter profiles and propidium iodide staining ...
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bioRxiv - Immunology 2021Quote: ... For the induction of inflammatory responses PD fluid treated mice received intra-peritoneal injections of 400 μL 4% Brewer modified thioglycollate medium (BD Biosciences, San Jose, CA) or sterile saline as control ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed twice in PBS and then incubated at room temperature for 10 minutes in 1x FACS Lyse (BD Biosciences, San Jose, CA). Following one wash with FACS buffer ...
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bioRxiv - Immunology 2020Quote: ... the transduced T cells were resuspended in 200 μl of FACS buffer and tetramer-positive T cells were sorted at the UW Department of Immunology Flow Cytometry Core using a FACS Aria II (BD Biosciences, San Jose, CA) cell sorter equipped with blue (488 nm) ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μl of each sample was analysed in duplicates using the BD™ CBA Human Soluble Protein Master Kit (#556264; BD Biosciences, CA, USA) alongside the BD™ CBA Human Flex Sets for interleukin 1ß (IL-1ß ...
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bioRxiv - Neuroscience 2023Quote: ... cells were single-sorted into 96-well plates containing complete media + ISRIB using a BD Biosciences FACSAria Fusion (BD Biosciences, San Jose, CA, USA). Clones were expanded and genomic DNA was sequenced to confirm the desired mutations ...
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bioRxiv - Synthetic Biology 2023Quote: Cell fluorescence was assayed on a BD Accuri C6 flow cytometer equipped with a 20 mW 480 nm solid state blue laser (BD Biosciences, San Jose, CA) or BD LSR Fortessa flow cytometer (BD Biosciences ...
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bioRxiv - Physiology 2023Quote: ... Images were then acquired using a Zeiss Axiovert 200 confocal microscope equipped with a 20× objective and CARV II confocal imager (BD Bioscience, San Jose, CA). 100 images were acquired ...
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bioRxiv - Immunology 2023Quote: ... and sorted for memory CD4+ T cells (CD4+CD44hiCD62Llo) that were either GFP+ (IL-10+) or GFP- (IL-10-) by a FACSAria flow sorter (BD Biosciences, San Jose, CA).
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with fluoresceinated antibodies against (TCRý, CD4, CD8, CD3, CD3χ, CXCR5, PD1, and intracellularly for ZAP70 and IL-10) (BD Biosciences, San Jose, CA) and data acquired on a FORTESSA flow cytometer and analyzed using FlowJo Software (Ashland ...
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bioRxiv - Molecular Biology 2022Quote: ... for 30 min at room temperature and analyzed by flow cytometry using a Becton Dickinson FACS Calibur flow cytometer (BD Biosciences, San Jose, CA). Cell cycle data were analyzed using CytExpert (Beckman Coulter Inc ...
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bioRxiv - Molecular Biology 2022Quote: ... crosslinked lungs were cut into small pieces using a razor blade then dissociated into single cell suspensions using Medicon filters (BD Biosciences San Jose, CA), washed with cold 1X PBS and 1 mM PMSF four times and pelleted by microcentrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed and re-suspended in ice-cold PBS prior to analysis using a FACSCanto II flow cytometer (BD Biosciences San Jose, CA) and data were then analyzed with FlowJo software (v 10.8.1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Breast tumor NOS2 and COX2 expression was analyzed previously by IHC using 1:250 diluted NOS2 antibody and 1:50 diluted COX2 antibody (no. 610328 and 610204, respectively, BD Biosciences, San Diego, CA,) and scored by a pathologist (4 ...
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bioRxiv - Cancer Biology 2023Quote: ... Breast tumor NOS2 and COX2 expression was analyzed previously by IHC using 1:250 diluted NOS2 antibody and 1:50 diluted COX2 antibody (no. 610328 and 610204, respectively, BD Biosciences, San Diego, CA,) and scored by a pathologist (49 ...
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bioRxiv - Cell Biology 2024Quote: ... namely anti-CD3-APC (1:5 dilution, clone SK7) and anti-CD45-PerCP (1:10 dilution, clone 2D1) (both from BD Biosciences, San Jose, CA). After incubation for 30 min at 4°C in the dark ...
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bioRxiv - Immunology 2024Quote: ... Purified naïve CD4+ T cells were stimulated with 2.5 μg/ml of anti-CD3e (#145-2C11, Cytek Biosciences, Fremont, CA) and 1 μg/ml of anti-CD28 (#553294, BD Biosciences, Franklin Lakes, NJ) antibodies in different culture conditions ...
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bioRxiv - Neuroscience 2024Quote: ... mCherry+ IPSCs were then manually isolated and expanded over several cycles to increase the percentage of mCherry+ IPSCs in culture before finite isolation by fluorescent cell activated sorting (FACS) using Influx cell sorter (BD Biosciences, San Diego, CA) with subsequent plating and expansion ...
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bioRxiv - Cell Biology 2023Quote: ... and mNeonGreen positive cells were sorted at the Stanford Shared FACS facility using a BD FACSAria II Cell Sorter (BD Biosciences, San Jose, CA) equipped with a blue 488 nm laser with a 502 nm long pass mirror and 525/50 nm filter.
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bioRxiv - Microbiology 2023Quote: ... The cells were washed with PBS+FBS and incubated with the monoclonal antibody anti-mouse CD8 T conjugated to Fluorescein Isothiocyanate (FITC) (BD-Pharmingen, San Diego, CA), which was suitable for determining the phenotype of CD8+ T cells ...
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bioRxiv - Biophysics 2024Quote: ... HeLa cells cultured in media composed of different percentages of D2O were analyzed with flow cytometry after staining with propidium iodide (PI/RNase, BD Biosciences, San Diego, CA), which easily penetrate dead or damaged cells (see Supplementary Fig ...
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bioRxiv - Biochemistry 2023Quote: Invasion assays were done in 8 μm pore size of the Chemotaxicell chamber coated with 100 μg/ml Growth factor reduced Matrigel (BD Biosciences, San Jose CA) for 3 h at 37°C and blocked with 0.1% bovine serum albumin in PBS ...
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bioRxiv - Microbiology 2023Quote: Surface markers of splenocytes were identified using monoclonal antibodies in conjunction with two immunofluorescence analysis with a flow cytometer (BD Accuri C6 Plus: BD, Mountain View, CA, USA). Antibodies used were Fluoroscein isothiocyanate (FITC)-conjugated anti-mouse CD4 and CD21/CD35 (CR2/CR1 ...