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Citations for Becton, Dickinson and Company :
2301 - 2350 of 2403 citations for 6 Chloro N methyl 5 nitro 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... spleen cells were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug™, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 monoclonal antibody (mAb ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and centrifuged at 1000rpm for 5-10min and stained with APC-Cy™7 Rat Anti-Mouse CD3 (clone: 17A2, Cat # 560590, BD Biosciences), FITC Rat Anti-Mouse CD4 (Clone ...
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bioRxiv - Physiology 2020Quote: ... Microtubules were allowed to grow from SVP in DMEM-F12+10% FBS for 5 days before staining with an anti-CD31 antibody (BD Biosciences, 557355). Images of microtubule outgrow from SVP were acquired with a Leica SP5-SMD confocal microscope ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell pellet was suspended with FACS medium and incubated with 5 μl of Alexa Fluor 488-conjugated anti-alkaline phosphatase (BD Pharmingen, 561495) and 5 μl of APC-conjugated anti-c-KIT (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... maintained for 20 min on ice and then homogenized with syringe every 5 min (BD Microlance™ 3 Needle 0,5 × 25 mm). Nuclear lysate supernatants were collected at 1000 rpm for 10 min at 4°C 43.
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bioRxiv - Microbiology 2022Quote: ... Colonies were grown from frozen stocks by streaking on TSA-II agar plates supplemented with 5% sheep blood (BD BBL, NJ, USA). Unless otherwise stated ...
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bioRxiv - Neuroscience 2023Quote: ... for 10 min at 37 °C and 5 % CO2 before they were stained with Fixable Viability Stain 510 (1:100, #564406, BD Horizon, USA) for 15 min at room temperature (RT ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were stained for 30 min on ice with 5 μl of biotin-labeled anti–cleaved caspase-3 monoclonal antibody (BD Biosciences, #550821). The cells were washed in Perm/Wash buffer (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... and Parabacteroides distasonis were grown anaerobically at 37°C in brain heart infusion (BHI) media (Oxoid, UK) supplemented with hemin (5 mg/L) and vitamin K (0.5 mg/L) (BD, Franklin Lakes, NJ). Lactobacillus johnsonii and Ligilactobacillus murinus were grown anaerobically at 37°C in de Man Rogosa Sharpe (MRS ...
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bioRxiv - Microbiology 2023Quote: BALF samples were centrifugated at 300 x g for 5 min and the pellets were resuspended in 500 μl Pharm Lyse Buffer (BD Biosciences, 555899) for 3 min at room temperature to lyse red blood cells ...
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bioRxiv - Microbiology 2023Quote: BALF samples were centrifugated at 300 x g for 5 min and incubated in 500 μl Pharm Lyse Buffer (BD Biosciences 555899) for 3 min at room temperature to lyse red blood cells ...
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bioRxiv - Microbiology 2023Quote: ... We then sheared the flagella by repeated passage through two 5-ml syringes connected by 22-inch-long tubing (ID 0.023”, BD INTRAMEDIC, BD Medical), about 30 times ...
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bioRxiv - Bioengineering 2023Quote: ... at 10 × 106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences, US) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Immunology 2023Quote: The proliferation of PBT was induced via CD3 antibody (20 ng/ml, clone: OKT3) and CD28 antibody (5 µg/ml, BD Biosciences 555725) bound to the surface of a 96-well Nunc MaxiSorpTM microtiter plate ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine staining was done by standard techniques following 5-hour stimulation with Gp61-80 peptide and Brefeldin A (GolgiPlug™, BD Biosciences). No peptide controls were treated under the same conditions supplemented with Brefeldin A but without Gp61-80 peptide ...
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bioRxiv - Cell Biology 2023Quote: ... Then cells were washed twice with HBSS-5%FBS and once with PBS and analysed by flow cytometry (BD FACScalibur, BD Bioscience).
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bioRxiv - Bioengineering 2023Quote: ... resuspended in 100 μL of PBS containing 5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5% goat serum) for 1h at RT and then stained with EC specific CD31 antibody (BD biosciences, 550274, 1:25 dilution) or muscle base membrane laminin antibody (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... Cells with the highest 2-5% GFP signal were selected two to three days post transfection using a flow cytometer (BD FACSAria II). Single cells were cultured in RPMI with 10% FBS ...
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bioRxiv - Genomics 2023Quote: ... were then sorted into a 384-well plate containing 5 μL of autoclaved source water using FACS (BD FACSMelody™ Cell Sorter, BD Biosciences). 10 µL of RLT+ lysis buffer (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... we prepared a cranial window assembly by activating the surface of a circular glass coverslip (5 mm diameter, 0.13-0.15 mm thickness; Assistent Karl Hecht) with a laboratory corona treater (BD-20ACV Electro-Technic Products). We then fused the activated glass coverslip to a cylindrical silicone plug (1.5 mm diameter ...
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bioRxiv - Immunology 2023Quote: ... and stained for the following surface markers on ice for 30 min: CD4 (APC-Cy7, 1:100, clone RM4-5, BD Biosciences, 565650), CD8b (Alexa Fluor 488 ...
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bioRxiv - Cancer Biology 2023Quote: ... of 6 weeks old were inoculated subcutaneously (s.c.) with 5 × 105 cells mixed 1:1 with BD Matrigel Matrix High Concentration (BD Biosciences, Erembodegem, Belgium) previously diluted 1:1 in culture medium ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked with 5% skimmed milk in TBS-Tween and incubated with anti-αSyn clone 42 (BD Biosciences, 1:1,000 dilution). In a slot blot ...
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bioRxiv - Microbiology 2024Quote: ... the suspensions of Tol 5 and its mutants were placed into a 96-well polystyrene (PS) microplate (353072; Becton, Dickinson and Company) and a 96-well glass plate (FB-96 ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were then stained for 30min at 4°C with the following antibodies: monoclonal anti-human CD33 V450 (WM53; BD Biosciences, San Jose, CA, USA); HLA-ABC FITC (W6/32 ...
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bioRxiv - Immunology 2021Quote: ... or PMA + ionomycin in the presence of brefeldin A for 4 h and stained using the Cytofix/Cytoperm Kit (BD Bioscience, Franklin Lakes, NJ, USA). Foxp3-positive cells and apoptotic cells were detected using the BD Foxp3 Staining Kit (BD Bioscience ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... Cells were then incubated for 1h at RT (or left at 4°C overnight) in the Iridium-intercalator solution in fixation and permeabilization buffer (BD Cytofix/Cytoperm™, BD Biosciences). Then cells were washed with 1x permeabilization buffer (BD Perm/Wash™ ...
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bioRxiv - Immunology 2019Quote: ... Cells were stained for 30 min at 4°C under darkened conditions with the following fluorochrome labeled monoclonal antibodies: anti-CD4-AmCyan (Clone SK3, BD Biosciences, Franklin Lakes, Nj, USA), anti-CD25-APCCy7 (Clone M-A251 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were rinsed with PBS and incubated for 20 hours at 4°C in primary antibody solution 1:3000 mouse anti-Ki67 (BD Biosciences, San Jose, CA, USA), 2% normal horse serum ...
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bioRxiv - Microbiology 2023Quote: ... purified gametocytes from DFsc7 or DFsc7;ΔRbpm1 lines were suspended in GMB at 4°C and sorted using a BD FACS AriaIII instrument (BD Biosciences, San Jose, CA, USA) based on their GFP and mCherry fluorescence intensity for male and female gametocytes ...
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bioRxiv - Immunology 2023Quote: ... The resulting single-cell suspensions were used at a concentration of 4 million cells/mL and stained with Fixable Viability Dye eFluor 780 (BD Bioscience, 565388, San Jose, USA), followed by Fc Block incubation and labelling with fluorochrome-conjugated antibodies (CD45-PerCP-Cy5.5 ...
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bioRxiv - Plant Biology 2020Quote: ... and 1 ml of cell suspension was incubated with 2 μl of Nile red solution (0.25 mg ml−1) for 5 minutes before the analysis with a bench flow cytometer (BD Accuri C6, BD Biosciences). To determine Nile red fluorescence ...
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bioRxiv - Molecular Biology 2020Quote: ... then were seeded into a 96-well plate at a density of 5×103cells/well coated with 10mg/ml Matrigel matrix without growth factor (Cat No: 3432-010-01, BD Biosciences, CA, USA) for 6 hour incubation at 37□ ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were incubated for 60 minutes in 1% BSA and 5% goat serum in PBS and then incubated overnight with anti-p21 antibody (BD Pharmingen, mouse, #556431) and anti-p16 antibody (abbiotec ...
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bioRxiv - Cancer Biology 2020Quote: ... 2000 cells were plated in 8-well chamber slides onto a base layer of growth factor-reduced Matrigel (5 mg/ml, BD Biosciences, Lot A6532) and collagen type I (1.5-2.1 mg/ml ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The seeded cells (5 x 104 cells/mL) were pre-stained with 1 μL Orange NO probe (BD Biosciences, San Jose, CA, USA) per 500 μL cell suspension for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Assay plates were incubated overnight at 37ºC in a 5% CO2 incubator and developed using BD ELISPOT AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISPOT reader and Immunospot software (Cellular Technology ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then blocked in PBS + 5% BSA for 1 hour before addition of primary antibodies (anti-LC3A/B, CST #4108, 1:100; anti-LAMP1, BD #555798, 1:100) diluted in blocking buffer overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Colonies were grown from frozen stocks by streaking on TSA-II agar plates supplemented with 5% sheep blood (BD BBL, New Jersey, USA). Unless otherwise stated ...
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bioRxiv - Microbiology 2021Quote: ... or LB media (10 g/liter Bacto tryptone, 5 g/liter Bacto yeast extract, 10 g/liter sodium chloride; BD, San Jose, CA) at 30°C ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with PerCP/Cy5.5 or BV421-conjugated anti–mouse CD4 rat monoclonal antibody (RM4-5; BD Biosciences, Franklin Lakes, NJ, USA). The cells were then fixed and permeabilized with Cytofix/Cytoperm reagent (BD Biosciences ...
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bioRxiv - Plant Biology 2019Quote: Surface-sterilized seeds were germinated and grown for 5 days containing 1% w/v sucrose and 1% w/v agar (Difco Bacto agar, BD laboratories; http://www.bd.com) in a growth chamber (NK system ...
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bioRxiv - Microbiology 2019Quote: Colonies were grown from frozen stocks by streaking on TSA-II agar plates supplemented with 5% sheep blood (BD BBL, New Jersey, USA). Colonies were then picked and inoculated in fresh Columbia broth (Remel Microbiology Products ...
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bioRxiv - Microbiology 2021Quote: ... Off-line samples were taken from the reactors each 5-10 h and the analysis of the GFP expression levels was performed with a BD Accuri C6 (BD Biosciences, CA, USA) based on FL1-A channel (excitation 488 nm ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibody incubated for one hour at RT in 5% milk TBS-T at 1:1,000 (HRP-Anti-Mouse, BD Pharmigen, Goat Polycloncal, 554002). Between steps 3 × 5 minute washes were performed in TBST ...
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bioRxiv - Neuroscience 2021Quote: ... the BECs were seeded in 96-well plates coated with collagen type I (5 µg/mL, #354231, BD Bioscience, San Jose, CA, USA) at a density of 104 cells per well ...
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bioRxiv - Immunology 2021Quote: ... were resuspended in defRPMI-1640 with 3% newborn calf serum and Benzonase™ (FACS buffer) in 5 mL FACS tubes and pre-incubated with GolgiStop™ (BD Biosciences) for ∼60 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... both ends of femurs and tibiae were cut with surgical scissors and bone marrow was extracted by flushing 3-5 ml sterile and ice-cold buffer through the bones using a 26G needle (BD Microlance 3 300300). The isolated bone marrow was then filtered through a 40 µM nylon cell strainer (FALCON 352340 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were stimulated with the RBD peptide pool (5 μg.ml−1 final concentration) and brefeldin A (1 μg.ml−1 ; BD Pharmingen, San Diego, CA, USA) at 37 °C overnight ...