Labshake search
Citations for Becton, Dickinson and Company :
1901 - 1950 of 2403 citations for 6 Chloro N methyl 5 nitro 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... the resulting egg rafts were placed in 27.0 × 19.4 × 9.5 cm plastic pans with 800ml of dechlorinated tap water and were supplied with 0.16g of liver powder (BD DifcoTM Desiccated ...
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bioRxiv - Immunology 2021Quote: ... for 1 hour at −20 ºC and finally stained for phosphorylated Stat-5 (BD biosciences, clone 47/Stat5; a647) in PBS/10% FBS for 30 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... cells expressing the highest and lowest 5-10 % LysoTracker Blue staining were sorted using a FACSARIA-SORP (BD Biosciences). CTL were used for standard over-night killing assays on the day of cell ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... The chamber with immobilized MTs was rinsed with Block + OSM (0.32 mg/ml glucose oxidase, 55 µg/ml catalase, 5 mM DTT, 25 mM glucose, 50 mM KCl, BD), and then 0.05 nM GFP-Kif18B ± 0.2 nM importin α-His6/His6-S-importin β or His6-importin α-mCherry/His6-S-importin β in Block + OSM was introduced and imaged immediately using total internal reflection fluorescence (TIRF ...
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bioRxiv - Cancer Biology 2022Quote: ... and flowed through 15 micron filters (PluriSelect) using back- pressure from an attached 5 mL luer-lock syringe (BD) (5mL cell suspension/per filter) ...
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bioRxiv - Bioengineering 2022Quote: ... droplets were merged by treating the PDMS device with corona for 5 seconds using a handheld corona treater (BD-20AC ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in PBS/0.1% Tween-20 and incubated with anti-HIF-1α (BD Biosciences), anti-β-Actin (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were then washed with PBS and resuspended in buffer assay red containing 5 μg/mL JC-1 (BD) and 10 000 events were acquired by flow cytometry (FACSCalibur ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 1 g of feces or 5 g of each tissue sample were added to tetrathionate broth (BD DifcoTM) and were incubated overnight at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Cells were then centrifuged (350g, 5 minutes) and resuspended in 1X Red Blood Cell Lysis solution (555899, BD Bioscience) for 15 minutes at RT ...
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bioRxiv - Immunology 2024Quote: ... NK cells were added to plates at a concentration of 2.5E+5 cells/mL in R10 media supplemented with anti-CD107a–phycoerythrin (PE)–Cy5 (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... resuspended in binding buffer and stained with 5 uL of FITC-AnnexinVfor 15 min in the dark according to manufacturer’s protocol (BD Biosciences-FITC annexin V apoptosis detection kit) ...
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bioRxiv - Immunology 2024Quote: ... anti-MASP-2 mAb F3-5 (Peter et al. 2022) and an irrelevant mouse IgG1 mAb (BD Biosciences, 557273) were coupled to to Dynabeads M-280 streptavidin (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated for 5 minutes on ice with rat anti-mouse CD16/CD32 (clone 2.4G2; BD Biosciences) at 1:100 dilution and 0.5% bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... PE-Cy7-anti-CD4 (clone RM4-5) and BV711-anti-CD8 (clone 53-6.7) mAbs (BD Biosciences or eBioscience). Cells were washed in FACS buffer ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... Villejuif) were engrafted with 5 x 106 Skmel5 cells in 200 µl of DPBS-50% Matrigel (BD Biosciences, #354234) on the right flank ...
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bioRxiv - Immunology 2023Quote: ... OncoImmunin Inc.) was added (5 min. at RT) and cells were acquired with a FACScan flow cytometer (BD BioSciences). The percentage of double positive eFluor+ and granzyme B+ target cells was calculated.
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bioRxiv - Immunology 2023Quote: ... β1 integrin blockade was performed with Purified NA/LE Hamster Anti-Rat CD29 (clone Ha2/5, BD Pharmingen, 555002) and the isotype control antibody Purified NA/LE Hamster IgM ...
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bioRxiv - Microbiology 2023Quote: Helicobacter pylori 26695 and isogenic derivatives were grown on trypticase soy agar plates supplemented with 5% sheep blood (BD) at 37°C with 5% CO2 ...
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bioRxiv - Pathology 2023Quote: ... single cells were sorted into an eppendorf containing PBS with 5% FCS using the FACSAria III sorter (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... PBMC were incubated overnight followed by addition of soluble anti-CD3 (5 μg/mL; catalog no. 555336, BD Pharmingen) to allow PBMC activation prior to T-cell mediated tumor cell killing assay ...
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bioRxiv - Immunology 2023Quote: ... 5 x104 – 2 x105 leukocytes were examined per sample with a FACSCanto II (BD Biosciences, Franklin Lakes, NJ, USA), according to established multi-color protocols (Cossarizza et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... GPIIIa, Luc.H11-FITC, #M031-1 or Integrin ⍺ 5/CD49e, Tap.A12-FITC and anti-human CD61-PE, #555754, BD Biosciences) and incubated for 15 min at RT in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in FACS buffer and incubated in FC Block for 5 minutes (1:60, BD Pharmingen, 553142) and primary antibodies for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... Isolated cells were centrifuged (350g, 5 minutes) and resuspended in 1X Red Blood Cell Lysis solution (555899, BD Bioscience) for 15 minutes at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in 2% BSA in PBS and 5 x 105 events measured per sample using a LSRFortessa Flow Cytometer and FlowJo v10.9.0 (BD Biosciences).
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bioRxiv - Neuroscience 2024Quote: ... Samples were fully lysed by pipetting up and down ∼5 times with a fine needle syringe (BD Medical, #328438) and vortexed for 10 seconds ...
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bioRxiv - Molecular Biology 2024Quote: Blood collection was performed during routine blood donation from a satellite bag into serum (5 mL, BD Vacutainer, UK) and plasma (anticoagulant ethylenediaminetetraacetic acid ...
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bioRxiv - Immunology 2024Quote: ... and incubated with FITC-conjugated anti-CD4 (RM4-5 or GK1.5 from BD Biosciences, San Diego, CA; 5µg/mL) and PerCP-conjugated anti-B220 (RA3-6B2 from BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Slides were treated for 10 minutes in H2O2 and after blocking in 10% goat serum in 0.1% Tween20 for 30 minutes and incubated overnight at 4°C with specific antibody diluted in PBS 0,1%Tween20: anti-mouse Ly6G (1A8, BD Pharmingen, cat 551459), or anti-mouse Ki67 (D3B5 ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hr at room temperature and incubated overnight at 4° C with primary antibodies against Syn1 (mouse, 1:1000, BD Biosciences, #610786), pSer129 (rabbit ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... Cells were then incubated for 1h at RT (or left at 4°C overnight) in the Iridium-intercalator solution in fixation and permeabilization buffer (BD Cytofix/Cytoperm™, BD Biosciences). Then cells were washed with 1x permeabilization buffer (BD Perm/Wash™ ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4 μL of the saturated culture were transferred in 60 uL PBS and the samples were stored at 4 °C for 1-2 days before being assayed by flow cytometry (BD FACSCanto II). Data were analyzed in FlowJo ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed once with wash buffer 1 and incubated for two days at 4°C with mouse anti-SR antibody (1:1,000; BD Biosciences; cat #612052). Sections were washed with wash buffer 2 (2% BSA ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were collected and fixed in 4% paraformaldehyde prior to acquisition on a BD LSRFortessa flow cytometer (BD Biosciences, San Jose, CA). CFSE signal intensity of 1 × 104 cells was recorded and analyzed to measure cell divisions ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for 1 hour at 4 °C with 2 µl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Bioengineering 2020Quote: ... was applied for 30 minutes at 4°C and stained cells were resuspended in FACS buffer before flow analysis on a BD FACSCanto™ II (BD Biosciences). Gating and flow data were processed on FlowJo (FlowJo LLC).
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bioRxiv - Molecular Biology 2021Quote: ... Between 2 - 4x105 events were collected per sample within 3 hours after staining on an LSRFortessa (BD Biosciences, 4 lasers, 18 parameters) and analyzed using FlowJo software (version 9.7.4 ...
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bioRxiv - Microbiology 2021Quote: ... and several ten-fold dilutions (10−4 to 10−7) were plated onto de Man Rogosa and Sharpe (MRS) media (BD Difco, USA) supplemented with 50 mg/L of mupirocin (PanReac AppliChem ...
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bioRxiv - Immunology 2021Quote: ... Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII flow cytometer (BD Bioscience, San Jose, CA).
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bioRxiv - Immunology 2021Quote: ... Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA).
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bioRxiv - Molecular Biology 2020Quote: ... Then the syndecan-4 expression on HUVECs was measured by flow cytometry with a BD Biosciences LSR II (BD Biosciences, CA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... and stained for 1 hour at 4 °C with 2 μl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Immunology 2022Quote: ... at 4°C for 5 min before surface staining with a cocktail of the following antibodies for 20 min at 4°C: CD3 V450 (Clone 17A2) (BD Cat. 561389), CD4 BV750 (Clone H129.19 ...
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bioRxiv - Microbiology 2019Quote: ... The resulting solutions were diluted (10-1 - 10-4) and 10 µl were seeded in plates containing potato dextrose agar (PDA) (BD-Difco®) and Sabouraud agar (SBA ...
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bioRxiv - Neuroscience 2021Quote: ... A 1ml astrocyte cell suspension of 4×104 cells/ml was plated in three chambers of four-chamber glass slides (BD Falcon, USA) coated with poly-D-lysine ...
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bioRxiv - Immunology 2020Quote: ... and cells were incubated for 4 h at 37 °C in the presence of BD GolgiPlug™ protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody and a dead cell marker (Live/Dead Fixable Aqua Dead Cell Stain Kit) ...
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bioRxiv - Immunology 2020Quote: ... in house production), 10 μg/mL α-IL-4 (cl. 11B11, in house production) and 10 ng/mL IL-12 (BD Pharmingen) and cultured on goat α-hamster IgG (MP Biochemicals ...
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bioRxiv - Immunology 2019Quote: ... Cells were resuspended in PBS/5%FBS then fixed in an equal volume of 1% paraformaldehyde and stored in the dark at 4°C prior to analysis on a BD LSR FortessaTM or LSR2 flow cytometer (BD Biosciences, UK). Data were analysed using FlowJo software (FlowJo LLC ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed at 4 °C for 20 min and washed in permeabilization buffer from the Fix/Perm kit (BD Biosciences, 554714). Antibodies against intracellular targets were diluted in permeabilization buffer and cells were incubated at 4 °C for 30 min followed by another wash prior to assessment with BD LSR Fortessa SORP cytometer ...