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Citations for Becton, Dickinson and Company :
2051 - 2100 of 2403 citations for 6 Chloro N methyl 5 nitro 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The worms were immobilized with levamisole (final concentration 200 µM) and cut open using with a pair of 30G 5/8” needles (PrecisionGlide, BD) in a scissors motion to extract the tissues ...
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bioRxiv - Immunology 2024Quote: ... Tregs were then sorted on a BD FACS Aria Fusion based on viability dyenegative CD4+ (BV605, clone RM4-5, BD) GFP-FOXP3+ ...
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bioRxiv - Immunology 2024Quote: ... live EGFP+ or EGFPneg Tcf7+/EGFP P14 CD90.1+ TE (Zombie Aquaneg EGFP+ or EGFPneg CD8a+ CD90.1+) from helped or helpless spleens were sorted on a 5-laser FACS Aria II sorter (BD Biosciences). Small aliquots of sorted samples were checked for purity (on average >98%) ...
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bioRxiv - Immunology 2024Quote: ... cells were treated for 5 hours with a protein transport inhibitor containing brefeldin A (BD Biosciences, Franklin Lakes, NJ, USA), washed and fixed for 10 minutes at 4°C with fixation/permeabilization solution (BD Cytofix/Cytoperm kit ...
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bioRxiv - Immunology 2024Quote: ... Mouse peripheral blood was collected via submandibular bleed using Goldenrod 5 mm animal lancets (MEDIPoint) into K2EDTA microtainer tubes (BD). For all specimen types ...
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bioRxiv - Microbiology 2024Quote: ... A single isolated colony was then inoculated in 5 ml of BHI broth (BD, cat. no. 237500, Franklin Lakes, NJ) in a 15 ml conical tube (VWR ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After 48h cultures were diluted and plated on Nutrient Agar plates (5 g/L peptone BD difco, BD Bioscience; 3 g/L yeast extract BD difco ...
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bioRxiv - Developmental Biology 2023Quote: ... collected by centrifugation at 300 g for 5 min and passed through a 35-µm cell strainer filter (BD Biosciences). To assess viability ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were pelleted for 5 min at 650 g and resuspended in PBS to be immediately analyzed using a Becton Dickinson (BD) LSRFortessa ...
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bioRxiv - Microbiology 2023Quote: ... We then sheared the flagella by repeated passage through two 5-ml syringes connected by 22-inch-long tubing (ID 0.023”, BD INTRAMEDIC, BD Medical), about 30 times ...
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bioRxiv - Cell Biology 2023Quote: ... and stained in 100 µL of FACS buffer (HBSS with 0.1% BSA w/v, 5 mM EDTA) containing mouse antibodies anti-CD45 (BD Sciences), anti-CD11b (Biolegend) ...
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bioRxiv - Microbiology 2023Quote: ... Experimental starters were prepared from frozen master stocks plated on 5% Columbia blood agar plates (Biomerieux ref no.43041) and grown overnight at 37°C with 5% CO2 prior to outgrowth in Todd-Hewitt (BD) broth supplemented with 50mM HEPES (TH+H ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then washed using FACS Wash Buffer containing 1x D-PBS + 0.5% FBS + 2mM EDTA and permeabilized at room temperature for 20 minutes using 100µL of Perm/Wash I (BD # 557885), diluted 1:10 with 1x D-PBS ...
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bioRxiv - Cell Biology 2023Quote: ... AML cells were incubated at 37°C (in 5% CO2 and air) in 96-well round-bottom plates (BD Biosciences) at 5 to 10 × 103 cells per well containing various concentrations of the Bispecific T cell engager (generated from published sequences using methods similar to those reported previously60 ...
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bioRxiv - Genetics 2023Quote: ... and the pellet was resuspended in 300 µL PBS with 5% FBS for flow cytometry using a CantoII (speed high; BD).
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bioRxiv - Immunology 2023Quote: ... were co-cultivated with 5×105 IE1-CTLL cells for 5h at 37°C in the presence of brefeldin A (BD GolgiPlug ...
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bioRxiv - Immunology 2023Quote: ... Sorting was conducted at room temperature and sorted cells were collected in 5 ml round-bottom polystyrene tubes (BD Biosciences) containing 500 μl of complete medium (RPMI 1640 supplemented with 10% FBS and 1% penicillin/streptomycin) ...
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bioRxiv - Microbiology 2023Quote: ... Experimental starters were made from master glycerol stocks struck on 5% Columbia blood agar plates (Biomerieux Ref# 43041) and grown overnight at 37°C with 5% CO2 prior to outgrowth in Todd-Hewitt (BD) broth supplemented with 50 mM HEPES (Sigma ...
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bioRxiv - Genomics 2023Quote: ... Pools of ciliate cells (5 – 50 cells) were then sorted into a 384-well plate containing 5 μL of autoclaved source water using FACS (BD FACSMelody™ Cell Sorter ...
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bioRxiv - Genetics 2022Quote: The sorted cells were collected in 36 (3 samples × 12 bins) 5-mL polystyrene round-bottom centrifuge tubes (BD Falcon), each of which contained 500 μL of PBS ...
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bioRxiv - Immunology 2023Quote: ... detection antibody (IFNγ, BD Biosciences #51-1818KA; IL-17A, Invitrogen #88-7371-88; and IL-5, BD Biosciences #51-1806KZ) was diluted in ELISpot diluent (eBiosciences ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for 3 hours at 37°C with 5% CO2 in R10 buffer supplemented with 0.33 μl/ml GolgiStop (BD Biosciences) and 15 μg/ml DNase I ...
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bioRxiv - Bioengineering 2023Quote: ... A tissue suspension was constituted by adding 5 mL of Tumor & Tissue Dissociation Regent (TTDR, BD Bioscience, San Jose, CA), and then incubating the mixture at 37 °C for 30 min with frequent agitation ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes containing the transferred proteins were rinsed and blocked in 0.1% Tween-20 in Tris-buffered saline (TBST) containing 5% nonfat milk (BD Biosciences) at RT for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... All dissociated cells were blocked with Fc receptors staining buffer (1% anti-mouse CD16/CD32, 2.4 G2, 2% FBS, 5% NRS, and 2% NMS in HBSS; BD Bioscience) and then stained with a standard panel of antibodies ...
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bioRxiv - Genomics 2023Quote: The following antibodies were used for Immunoblot analysis (Extended Data Table 5): Purified Mouse Anti-PTEN (BD Bioscience, Cat# 559600), Phospho-Akt (Ser473 ...
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bioRxiv - Immunology 2023Quote: ... high-binding 96-well plates were coated with 5 μg/ml anti-CD3 (Clone UCHT1, BD Pharmingen™ #555329, USA) in PBS (ThermoFisher Gibco™ #21600044 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After 48h cultures were diluted and plated on Nutrient Agar plates (5 g/L peptone BD difco, BD Bioscience; 3 g/L yeast extract BD difco, BD Bioscience ...
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bioRxiv - Physiology 2024Quote: ... Cells were then pelleted at 500 x g for 3 min at 4°C and resuspended in 25 μL of 5 mg/mL FcBlock (rat anti-mouse CD16/32, clone 2.4G2, BD Biosciences) diluted in FACS Buffer and incubated on ice for 10-15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... 2.5 % donkey serum in PBST containing 0.2% Triton X-100) and incubated with α-EPS8 antibody produced in mouse (BD Transduction Laboratories™ ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice (900 x g for 5 minutes at room temperature) and then 50µL of surface antibody cocktail diluted in Brilliant Violet buffer (BD Biosciences) added and incubated 20 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2024Quote: ... stained with 2.5 μM cisplatin for 5 min at 25°C and fixed using transcription factor buffer set (BD Biosciences), followed by intracellular staining for 60 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... and stored in FB 4C overnight before analysis on a 3 laser Aurora spectral flow cytometer (Cytek, figure 1D, Supplementary Figure 2) or X20 Special Order 5-lasers Fortessa (BD) flow cytometer (other figures) ...
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bioRxiv - Microbiology 2024Quote: ... The overnight cultures of Pseudomonas aeruginosa ATCC27853 and EXT111762 were adjusted to McFarland Standard 1 and further diluted to 5 × 105 cells mL−1 in cation adjusted Mueller Hinton II broth (BD). A volume of 5 mL was distributed to each cultivation flask and supplemented with either 0.5x ...
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bioRxiv - Immunology 2024Quote: ... according to manufacturer’s instructions to exclude dead cells and then labelled in FACS buffer with the following antibodies: V500 Rat anti-Mouse CD4 (RM4-5, BD), eF450 CD5 Monoclonal Antibody (53-7.3 ...
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bioRxiv - Neuroscience 2019Quote: ... injection with α-Ly6G or isotype control antibodies (IP 4 mg/kg; α-Ly6G, clone 1A8, No. 561005; Iso-Ctr, Rat IgG2a, κ, No. 553929; BD Biosciences). Animals were 9-10 months of age at the start of the experiment.
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bioRxiv - Cell Biology 2019Quote: ... for 2 hours at room temperature followed by overnight incubation at 4°C with anti-PECAM-1 (MEC13.3, BD Bioscience #550274, 1:250) diluted in blocking buffer ...
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bioRxiv - Genomics 2020Quote: ... TFA4 strain was cultivated in nutrient Zobell medium (4 g L−1 peptone and 1g L−1 yeast extract Becton, Dickinson and Company, Sparks ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then left at 4°C overnight in a blocking solution containing the primary antibody: mouse E-Cadherin (BD Biosciences, 1:1000) and mouse N-Cadherin (BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were allowed to recover for 2 days in 6-well plates filled with 4 ml medium per well before being FACS sorted for mCherry-expression on a BD FACS Aria III (BD Biosciences, Heidelberg, Germany). For subsequent limiting dilution cloning ...
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bioRxiv - Immunology 2021Quote: Control and SC-macrophages were fixed with 4% PFA for 10 mins and permeabilized with 1x perm wash buffer (BD cat#51-2091KZ) for 30 mins at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed cells were incubated overnight at 4 °C with primary mouse monoclonal anti-alpha synuclein antibody (Cat no. 610787; BD Biosciences; 1:200) and for 2 hr with secondary goat anti-mouse Alexa Fluor Plus 488 antibody (Cat no ...
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bioRxiv - Immunology 2022Quote: ... were added to the ELISpot plate containing an immobilized primary antibody to either IFN-g or IL-4 (BD Cat# 551881 and BD Cat# 551878, respectively), and were exposed to various stimuli (e.g ...
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bioRxiv - Microbiology 2021Quote: ... a 10 µl inoculating loop was used to streak cecal slurry (cecal contents in BPW) onto Xylose Lysine Tergitol-4 agar (BD Difco, Sparks, MD). supplemented with 4 ppm tetracycline for trial 1 and BGS supplemented 32 ppm ampicillin (amp ...
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bioRxiv - Immunology 2020Quote: ... Pellets were then stained with a panel of antibodies for 30 minutes (4°C) followed by washes in PBS before analysis by flow cytometry (BD, Franklin Lakes, NJ).
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated in the FC wash buffer (2h at 4 °C) containing FITC-coupled anti-CD44 antibody (1 to 80 BD Pharmingen Cat.No. 555478) or its isotype control (FITC Mouse IgG2b ...
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bioRxiv - Neuroscience 2020Quote: Brains from paraformaldehyde-perfused 12 month-old mice were sectioned and 30 µm free-floating sections were incubated overnight at 4°C with primary antibodies (anti-αsyn, BD Transduction, 1:700), anti-DNAJC5 (Millipore ...
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bioRxiv - Immunology 2020Quote: ... Cells were then spun down at 500 × g for 5 min at 4°C and resuspended with the following antibody-conjugated fluorophores for 20 minutes at room temperature: anti-CD3-BV421 (BD, 1F4, 1:100), anti-CD4-PE-Cy7 (BD ...
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bioRxiv - Cell Biology 2019Quote: ... stably transduced mApple and eGFP EpH4 cells were sorted using a BD 4 laser FACSAria III cell sorter (BD Biosciences, San Jose, CA), treated for 24 hours with 1 μM Cisplatin or PBS vehicle ...
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bioRxiv - Systems Biology 2021Quote: ... HeLa and RAW 264.7 cells were washed by chilled PBS and sorted on the nanoPOTS chips (4 × 12, 1.2 mm diameter per well) using the Influx II cell sorter (BD Biosciences, San Jose, CA) as described previously 14 ...