Labshake search
Citations for Becton, Dickinson and Company :
2001 - 2050 of 2403 citations for 6 Chloro N methyl 5 nitro 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... aureus JE2 USA300 (NARSA) and the clinical isolates were maintained on blood agar plates (Columbia agar +5% sheep blood, BD) and grown in Tryptic Soy Broth (TSB ...
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bioRxiv - Immunology 2022Quote: ... Samples were washed with twice with 4% FBS/PBS and stained with DAPI (0.1 μg/ml) for 5 minutes at room temperature prior sorting of live DAPI— cells on the AriaIII (BD Biosciences)
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were incubated on a cold block for 2-5 minutes before being plated in a 96 (12 x 8) grid on Omnitrays containing LB (BD, Franklin Lakes ...
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bioRxiv - Microbiology 2022Quote: ... For each sample 1.5 x 106 cells first underwent Fc receptor blockade with rat anti-mouse FcγRIII/II receptor (CD16/32; BD Biosciences). After Fc receptor blocking for 5 minutes on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were collected by centrifugation for 5 min at 400 × g and analyzed using a FACScan flow cytometer (BD Biosciences), which was equipped with an argon laser emitting at 488 nm ...
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bioRxiv - Cell Biology 2022Quote: ... All samples were measured with a FACSFortessa flow cytometer equipped with 5 lasers (355, 405, 488, 561 and 640 nm) and the FACSDiva software version 8.0 (BD Biosciences) was used.
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bioRxiv - Immunology 2020Quote: ... Mouse peripheral blood samples were drawn from the facial vein using Goldenrod 5 mm animal lancets (Medipoint; Mineola, NY) and collected into K3EDTA-coated tubes (BD). Erythrocytes were removed from all mouse tissue specimens using ammonium chloride-potassium bicarbonate (ACK ...
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bioRxiv - Microbiology 2021Quote: Overnight cultures were grown with shaking at 37° C in 5 mL of BHI (BD Difco Bacto Brain Heart Infusion) broth ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... The 100 μl of the resuspended solution was incubated with 5 µl of FITC Annexin V (BD Pharmingen™, 560931) in FACS tube for 15 min at RT (25°C ...
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bioRxiv - Neuroscience 2021Quote: ... The muscle layers were closed with sutures (Mersilk 5-0 Ethicon) and skin closed with Michel clips (9mm BD Diagnostics). Mice were monitored daily after surgery.
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bioRxiv - Microbiology 2021Quote: ... Cd strains were cultured anaerobically (85% N2, 10% CO2, 5% H2) on BHIS plates: Brain Heart Infusion Agar (BD Difco) supplemented with 0.5 mg/mL cysteine and 5 mg/mL yeast extract ...
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bioRxiv - Immunology 2021Quote: ... in the last 5 hours of culture a protein transport inhibitor Brefeldin A (1µL/mL) was added (BD Biosciences, USA). Cells were fixed using the BD Cytofix/Cytoperm® kit (BD Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... The following antibodies were used for immunoblotting: anti-SKAP55 rat mAb (SK13B635) anti-ADAP mAb (clone 5/FYB, BD Bioscience), anti-β-actin mAb (clone AC-15 ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were incubated in darkness for 5 min at room temperature and then analyzed using flow cytometry (BD LSRFortessa™). The acquired data was analyzed using FlowJo software (Ashland ...
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bioRxiv - Cancer Biology 2021Quote: ... Single-cell suspensions were stained with 5 μM Cell-ID Cisplatin (Fluidigm Corp.) and incubated with Fc block (BD Biosciences), followed by surface antibody cocktail ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were analyzed in a BD FACSAriaII cytometer using FacsDiva software (version 5, BD Biosciences, San Jose, CA, USA). Data analyses were performed with FlowJo software (version 10 ...
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bioRxiv - Molecular Biology 2022Quote: ... Each sample was transferred into a pre-coated 5 mL polystyrene flow cytometry tube and sorted using a 100uM nozzle in a FACSAria III Cell Sorter (BD Biosciences Inc. ...
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bioRxiv - Pathology 2022Quote: ... Gas samples were collected and stored in 5-mL additive-free glass vacutainers® (BD Vacutainer® Z. ref: 367624) and analyzed by gas chromatography within two weeks of sample collection ...
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bioRxiv - Immunology 2022Quote: ... T cells and tumor cells were co-cultured at a 1:1 effector to target (E:T) ratio for 5 hours in the presence of GolgiStop Protein Transport Inhibitor (BD Biosciences). Surface phenotype of cells was determined by flow cytometry using fluorochrome-conjugated antibodies specific for CD3 (Miltenyi Biotec ...
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bioRxiv - Immunology 2019Quote: Fresh breast tumor samples were cut into small pieces and gently triturated with a 5 mL syringe plunger on a 70 μm Cell-Strainer (BD) in RPMI-1640 medium (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell pellets were incubated in ACK lysis buffer for 5 minutes and rinsed twice in FACS buffer (BD Pharmingen, #554656). Tumor cells were strained through a 40 micron filter and resuspended to 1 x 107 cells / mL in FACS staining buffer ...
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bioRxiv - Cell Biology 2019Quote: Cells were stained as described and resuspended in DPBS with 1% FBS to a concentration of ∼5×106 cells/ml for ImageStream Mark II (Amnis) and ∼1×106 cells/ml for FACSAria I (BD) analysis ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... The liquid was poured off from the cells (leaving approximately 100 µl in each tube) and 5 µl of mouse Fc Blocking Reagent (rat anti-Mouse CD16/CD32 BD) was added to samples and incubated for 5 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... and the marker-specific fluorochrome-labeled antibodies rat anti-mouse CD4-AF700 (clone RM4-5, 1:200, BD cat#557956), rat anti-mouse CD8-APCy7 (clone 53-6.7 ...
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bioRxiv - Immunology 2019Quote: ... and human monocyte-derived macrophages were seeded for 1 h at 37°C and 5% CO2 in 24- or 48-well non-tissue culture treated plates (BD) at a density of 1-2 x 105 cells/ml ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 20 and 50μM BAM15 was applied to BBL trypticase soy agar with 5% sheep blood (TSAⅡ) (BD, Franklin Lakes, NJ). The number of colonies was counted after culture for 24 hours at 37°C.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were trypsinized using standard cell culture techniques and transferred into 5 mL polystyrene round-bottom tubes (#352052, BD Falcon). After PBS-washing and centrifugation (800 rpm ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 5 hours at 37C in the presence of brefeldin A (1 μl/ml, GolgiPlug, BD Biosciences), human recombinant IL-2 (10 U/well ...
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bioRxiv - Microbiology 2019Quote: Strains of interest were streaked on TSA-II agar plates supplemented with 5% sheep blood (BD BBL, New Jersey, USA). These streaked cells were then inoculated into fresh Columbia broth (normal or acidic ...
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bioRxiv - Genetics 2020Quote: ... we also collected 5-7 ml of peripheral blood in a tube containing the anticoagulant K3 EDTA (#6457, BD Biosciences) which was used for flow cytometry as described below ...
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bioRxiv - Immunology 2020Quote: ... for 5 min at RT prior to staining with a surface stain cocktail containing the following antibodies purchased from BD and Biolegend ...
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bioRxiv - Microbiology 2021Quote: ... and 5% v/v sheep blood (Sanbio, Uden, The Netherlands) (TSYA) or in tryptic soy broth (BD, New Jersey, USA) supplemented with 0.5% w/v yeast extract (TSYB ...
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bioRxiv - Plant Biology 2020Quote: In vitro mycelium diameter measurements were performed by depositing a 5 μl droplet of a 1.5 × 105 spores ml−1 solution onto 1.5 % granulated agar (BD Difco™) plates supplemented with homogenates of WL- or WL+FR-treated leaves (1 gFW ml−1) ...
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bioRxiv - Immunology 2021Quote: ... All samples were then incubated with Fc block (5 μg mL-1 CD16/CD32 (BD Biosciences, San Diego, CA, USA) and 0.1 % mouse serum in FACs buffer (PBS containing 0.5 % BSA and 2 mM EDTA (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... 2 × 105 PBMCs were added to each well (NK cells comprise 5-15 % of PBMCs) in the presence of monensin (GolgiSTOP™; 1:1,000 dilution; BD) and Brilliant Violet 786-labeled anti-Lamp1 mAb (clone SJ25C1 ...
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bioRxiv - Immunology 2020Quote: ... Cells were stimulated for 5 hours at 37C in the presence of brefeldin A (1 μl/ml, GolgiPlug, BD Biosciences), human recombinant IL-2 (10 U/well ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then blocked in 5% non-fat milk and immunoblotted with appropriate primary antibodies: mouse monoclonal anti-SorLA (1:5,000; mAb# 611860, BD Bioscience) for the detection of full length SorLA and SorLA2131 proteins ...
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bioRxiv - Immunology 2021Quote: ... The cells were cultured in a humidified incubator with 5% CO2 for 72h and analyzed using a BD FACS Canto flow cytometer (BD,) and FlowJo software (TreeStar ...
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bioRxiv - Cell Biology 2021Quote: ... Propidium Iodide (PI) (5 μg/ml) was added and kept for 15 minutes and subjected to FACS analysis (BD FACSAria).
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bioRxiv - Neuroscience 2022Quote: ... resuspended in 500 μL FACS buffer and transferred to 5 mL round-bottom FACS tubes through cell strainer caps (BD). Cells were sorted through a 100 μm nozzle on a FACS Aria II (BD ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were probed for non-glucosylated Rac1 in Tris -buffered saline containing 0.1% Tween (TTBS) with 5% milk and 1:1,000 mouse anti-Rac1 (Clone 102/Rac1 610650; BD Bioscience, USA) and then incubated overnight at RT and 6 h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Gates were determined using the isotype controls mouse anti-IgG2b κ (APC-conjugated, Clone 27-35, 1:5, BD Biosciences) and mouse anti-IgG2a κ (PE-Cy7-conjugated ...
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bioRxiv - Bioengineering 2022Quote: ... DOs-laden microspheres were incubated with Williams’ E medium (BasalMedia, L660KJ) plus 5 µM cholyl-lysyl-fluorescein (CLF, BD-451041) for 15 minutes at 37 °C and 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... HSP+TCR condition – cells cultured with IL-15 and -7 and activated by anti-CD3 (5 μg/ml, BD Biosciences) and CD28 (1 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... Plates were photographed after 24 h incubation at 37°C in 5% CO2 or under anaerobiosis (GasPak jar, BD Biosciences).
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bioRxiv - Microbiology 2022Quote: ... swabs of 0.5 MacFarland suspensions prepared from isolated colonies were inoculated for confluent growth on cation-adjusted Mueller Hinton agar with 5% sheep blood (BBL, Becton-Dickinson, Franklin Lakes, NJ) using discs (BD) containing conventional drug masses ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions were washed with 1XPBS-4%FBS before incubation for 20 min on ice at 5•107 cells/ml with 500 ng/ml Fc block (2.4G2, BD Pharmingen). Cells were then incubated for 1 hour on ice with AF700 conjugated CD45.2 monoclonal antibody (104 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells (1.5 x 106 cells) first underwent Fc receptor blockade with rat anti-mouse FcψRIII/II receptor (CD16/32; BD Biosciences). After blocking for 5 minutes on ice ...
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bioRxiv - Physiology 2024Quote: ... Cells were then pelleted at 500 x g for 3 min at 4°C and resuspended in 25 μL of 5 mg/mL FcBlock (rat anti-mouse CD16/32, clone 2.4G2, BD Biosciences) diluted in FACS Buffer and incubated on ice for 10-15 min ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice (900 x g for 5 minutes at room temperature) and then 50µL of surface antibody cocktail diluted in Brilliant Violet buffer (BD Biosciences) added and incubated 20 minutes at room temperature in the dark ...