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Citations for Becton, Dickinson and Company :
1551 - 1600 of 1656 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... cells were fixed using 4% PFA for 15 minutes at 4°C and permeabilized for intracellular cytokine staining using Perm/Wash Buffer (BD Perm/WashTM). For intracellular staining ...
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bioRxiv - Cell Biology 2021Quote: ... C-peptide and Glucagon labelled cells were detected on an LSRII flow cytometer with FACSDiva™ Software (BD Biosciences, San Jose, CA) using the 405- ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for 15 min at 37°C before incubation with primary antibodies Alexa Fluor 647 Rat Anti-mouse CD31 (BD Pharmigen cat:563608) for CD31 cell labeling and Alexa Fluor 647 Rat IgG2a,k Isotype control (BD Pharmigen cat:557690) ...
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bioRxiv - Immunology 2020Quote: ... and cells were incubated for 4 h at 37 °C in the presence of BD GolgiPlug™ protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody and a dead cell marker (Live/Dead Fixable Aqua Dead Cell Stain Kit) ...
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bioRxiv - Immunology 2020Quote: ... spleen cells were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug™, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 monoclonal antibody (mAb ...
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bioRxiv - Immunology 2020Quote: Cryopreserved lymphocytes from BAL were thawed and stimulated with H1N1pdm09 (MOI 1) or medium as a control for 18 hours at 37°C prior GolgiPlug (BD Biosciences, UK) addition as per manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: ... Cells were resuspended in PBS/5%FBS then fixed in an equal volume of 1% paraformaldehyde and stored in the dark at 4°C prior to analysis on a BD LSR FortessaTM or LSR2 flow cytometer (BD Biosciences, UK). Data were analysed using FlowJo software (FlowJo LLC ...
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bioRxiv - Immunology 2020Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop™, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed at 4 °C for 20 min and washed in permeabilization buffer from the Fix/Perm kit (BD Biosciences, 554714). Antibodies against intracellular targets were diluted in permeabilization buffer and cells were incubated at 4 °C for 30 min followed by another wash prior to assessment with BD LSR Fortessa SORP cytometer ...
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bioRxiv - Immunology 2022Quote: ... The Fc cell receptors were then blocked for 10 min at 4°C with an anti-CD16/CD32 antibody (10 μg/mL: BD Biosciences 553142) and the cells were resuspended in 25 μL of staining buffer ...
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bioRxiv - Neuroscience 2022Quote: ... GAS 2006 was cultured under static conditions at 37°C for 17 h in THY medium (Todd-Hewitt broth (Bacto BD, Cat# 249240) containing 1.5% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... for 10 min at 37 °C and 5 % CO2 before they were stained with Fixable Viability Stain 510 (1:100, #564406, BD Horizon, USA) for 15 min at room temperature (RT ...
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bioRxiv - Immunology 2022Quote: ... Next cells were fixed using BD Cytofix Fixation buffer for 10 minutes at 37°C and permeabilized using ice-cold Perm buffer III (BD Biosciences, 558050) for 30 minutes on ice ...
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bioRxiv - Physiology 2022Quote: ... Samples were stored at 10-15°C in 2 mL K2-EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 15 to 45 min later.
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bioRxiv - Physiology 2022Quote: ... The sample was stored at 10-12 °C in 2 mL EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 0.5-2.5 h later ...
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bioRxiv - Microbiology 2023Quote: ... at 37°C in a TC-7 roller drum (NB, Canada) at 240 rpm or on Lysogeny Broth (LB; BD Difco, Canada) agar plates ...
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bioRxiv - Immunology 2023Quote: ... Stimulated cells were washed once in FACS buffer before fixation for 20 min at 4°C using the BD Cytofix/Cytoperm solution and permeabilization using BD Perm/Wash™ buffer (BD Biosciences). Permeabilized cells were incubated for 30 min with antibodies (diluted 1:200 in BD Perm/Wash ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H: BD construct #8) and each column is a single AD construct (For example column 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... on ice and enzymatically digested with agitation for 30 minutes at 37°C using the BD TuDoR™ dissociation reagent (BD Biosciences). The obtained single-cell solution was sieved through a 70 µM cell strainer (Corning ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 10 ng/mL anti-CD3 mAb at 4°C overnight (Purified NA/LE mouse anti-human CD3, BD Cat# 557052). Three-fold serial dilutions of either IL-7 or a test compound were added to the cells and incubated for 4 days at 37°C ...
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bioRxiv - Immunology 2023Quote: The plate was incubated 6 hours at 37 °C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences) for the last 4 hours of culture ...
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bioRxiv - Cancer Biology 2023Quote: ... treatment was performed at 100μg/ml concentration in dark at 4°C for an hour and analyzed by FACS Calibur flow cytometer and processed using Cell Quest software (BD Biosciences, USA).
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bioRxiv - Genomics 2023Quote: Trypsinized or MACS sorted cells were incubated at 4°C for 20 min with APC-conjugated anti-CD56 antibodies (1:40, BD Pharmingen #555518) or isotype control (1:40 ...
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bioRxiv - Genomics 2023Quote: ... then washed three times with DPBS before permeabilization in ice cold Phosflow Perm Buffer III for 10mins at -20°C (BD Bioscience #558050). Before incubation with primary antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... for 20min at 4°C and stained with intracellular antibodies including anti-mouse IFN-γ-Pe-Cy7 and IL-2-BV605 (BD Bioscience) for 30min at 4°C ...
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bioRxiv - Microbiology 2024Quote: Lung cytokines were quantified from the supernatant of thawed organ homogenates (stored at - 80°C) from each experimental timepoint using the BD Mouse Th1/Th2/Th17 Cytometric Bead Array (CBA) Kit (BD Biosciences, 560485). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... and stained with the following conjugated antibodies (1/100 dilution in PBS) for 15 minutes at 4°C: CD44 (PE, BD Biosciences 555479), EpCAM (FITC ...
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bioRxiv - Immunology 2021Quote: Low-passage virulent Leptospira interrogans serovar Pomona was cultured at 28°C in Ellinghausen–McCullough–Johnson–Harris (EMJH) medium (BD Difco™, USA) supplemented with 10% bovine serum albumin (BSA) ...
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bioRxiv - Bioengineering 2019Quote: ... and Anoxybacillus flavithermus strain WK1 (DSMZ, Germany) cells were grown at 55°C in Caso Broth (15g/L of peptone from casein (BD, cat no. 211921), 5g/L of peptone from soybean (Sigma-Aldrich 70178-100G) ...
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bioRxiv - Cell Biology 2019Quote: ... for 2 hours at room temperature followed by overnight incubation at 4°C with anti-PECAM-1 (MEC13.3, BD Bioscience #550274, 1:250) diluted in blocking buffer ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then left at 4°C overnight in a blocking solution containing the primary antibody: mouse E-Cadherin (BD Biosciences, 1:1000) and mouse N-Cadherin (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed cells were incubated overnight at 4 °C with primary mouse monoclonal anti-alpha synuclein antibody (Cat no. 610787; BD Biosciences; 1:200) and for 2 hr with secondary goat anti-mouse Alexa Fluor Plus 488 antibody (Cat no ...
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bioRxiv - Microbiology 2022Quote: ... All Bifidobacterium isolates were streaked for purity and all strains were grown at 37°C in RCM and de Man Rogosa and Sharpe (MRS) (BD Difco™, BD Biosciences, USA) media in an anaerobic chamber (Whitley DG250 ...
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bioRxiv - Microbiology 2022Quote: Strains were streaked from -80°C freezer stocks (frozen in Brain Heart Infusion [BHI] broth with 15% glycerol) onto Brain Heart Infusion agar (BHIA; BD, Franklin Lakes, NJ), followed by incubation at 37°C for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... Mice recovered at 32°C (Warm Air System, Safety Cabinet Version red, VetTech, UK) until spontaneous movement and received 0.8mg/kg subcutaneous (BD Microlance 27G 13mm needle) atipamezole to reverse the ɑ-agonist into the neck scruff.
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bioRxiv - Immunology 2020Quote: ... Pellets were then stained with a panel of antibodies for 30 minutes (4°C) followed by washes in PBS before analysis by flow cytometry (BD, Franklin Lakes, NJ).
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bioRxiv - Neuroscience 2020Quote: Brains from paraformaldehyde-perfused 12 month-old mice were sectioned and 30 µm free-floating sections were incubated overnight at 4°C with primary antibodies (anti-αsyn, BD Transduction, 1:700), anti-DNAJC5 (Millipore ...
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bioRxiv - Immunology 2020Quote: ... Cells were then spun down at 500 × g for 5 min at 4°C and resuspended with the following antibody-conjugated fluorophores for 20 minutes at room temperature: anti-CD3-BV421 (BD, 1F4, 1:100), anti-CD4-PE-Cy7 (BD ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated for 30 min at 4 °C with a saturating concentration of anti-CD24a monoclonal antibody (eBioscience, clone M1/69) and anti-CD56 monoclonal antibody (BD Biociences, clone 809220) in the presence of anti-CD16/CD32 (eBioscience) ...
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bioRxiv - Cell Biology 2020Quote: ... cerevisiae cells were grown at 25°C in YPD liquid medium containing 1% (w/v) Bacto yeast extract (BD Biosciences, San Jose, CA), 2% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial cultures were routinely grown in lysogeny broth (LB) broth at 37°C with aeration or on LB solidified with 1.5% agar (BD Biosciences, San Jose, CA). When stated ...
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bioRxiv - Immunology 2020Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
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bioRxiv - Immunology 2022Quote: ... mice were euthanized and tumors were quickly harvested and fixed for 14-16 hr at 4&C in BD Cytofix/Cytoperm (BD Bioscience, Cat #: 554722) diluted 1:4 in PBS ...
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bioRxiv - Immunology 2022Quote: ... In-between incubation steps, cells were washed (300 x g, 10 min, 4°C) with 40 mL of BD Cell Wash (BD Biosciences, Allschwil, Switzerland). In order to block Fc receptors ...
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bioRxiv - Microbiology 2022Quote: ... Afterwards the samples were fixated and permeabilized for 20 min and 60 min at 4 °C in the dark using BD Cytofix/Cytoperm™ solution and BD Perm/Wash™ solution (BD Biosciences), respectively ...
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bioRxiv - Immunology 2023Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... overnight and then stimulated with 1 nM N4 in the presence of vehicle or Compound 182 (1 μM) for 4 h at 37°C in complete T cell media supplemented with BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were then stained with fluorophore-conjugated antibodies to cell surface CD8 and intracellular IFNγ ...
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bioRxiv - Cancer Biology 2023Quote: ... The bottom and top parts of the membrane were then incubated overnight at 4°C with primary p53 antibody (2 µg/mL, 1:250) (Clone DO-1, BD Biosciences, Cat. 554293) and primary vinculin antibody (1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... pre-coated for 1 hour at 37°C with anti-CD3 antibodies (5ug/mL, clone: 145-2C11, BD Biosciences, San Diego, CA, USA) and supplemented with soluble anti-CD28 antibodies (2ug/mL clone ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667, 1:100 in 1X BD Perm/wash solution) or anti-BST2 (Proteintech #13560-1-AP ...