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Citations for Becton, Dickinson and Company :
1351 - 1400 of 1656 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated overnight at 4 °C with primary antibodies anti-E-cadherin (mouse clone 36 BD610182, BD, Heidelberg, Germany), anti-paxillin (mouse clone 5H11 AHO0492 ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for 3 hours at 37°C with 5% CO2 in R10 buffer supplemented with 0.33 μl/ml GolgiStop (BD Biosciences) and 15 μg/ml DNase I ...
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bioRxiv - Microbiology 2023Quote: ... 1x105 CFU bacteria were incubated for 48h at 37°C in 0.2 mL cation-adjusted Mueller-Hinton broth (CAMHB) (BD Biosciences) containing increasing concentrations of antibiotic (0.25 mg/L to 256 mg/L for Nafcillin and Oxacillin ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated at 37°C for 4 hours after which they were fixed and permeabilized with the Transcription Factor Buffer Set from BD. γH2AX was detected with PerCP/Cyanine5.5 anti-H2A.X-Phosphorylated (Ser139 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were diluted in 3% BSA/PBST (0.1% Triton X-100 in PBS) and incubated with the samples at 37°C for 1 h: mouse anti-BrdU (BD Biosciences 347580 ...
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bioRxiv - Microbiology 2023Quote: ... Individual colonies were picked for overnight growth at 37◦C in 1.6mL LB (Miller) broth (BD, Franklin Lakes,NJ, USA) each ...
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bioRxiv - Microbiology 2023Quote: ... aureus and Corynebacterium strains were cultured overnight at 37°C with shaking in brain-heart infusion broth (BHI; BD Biosciences) unless otherwise noted ...
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bioRxiv - Molecular Biology 2023Quote: ... were coated overnight at 4 °C with 300 ng/mL mouse anti-human RIPK1 capture antibody (1:100 dilution in PBS of BD Bioscience BD610459 lot 0314064 ...
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bioRxiv - Biophysics 2024Quote: Bacterial strains stored at −80 °C were plated on Mueller–Hinton agar (MHA, BD Difco, BD, Franklin Lakes, NJ USA) and incubated at 37 °C overnight prior to use ...
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bioRxiv - Biophysics 2024Quote: Bacterial strains stored at −80 °C were plated on Mueller–Hinton agar (MHA, BD Difco, BD, Franklin Lakes, NJ USA) and incubated at 37 °C overnight prior to use ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and resuspendend in cRPMI and kept at 4°C for sorting on a FACSAria III (BD Bioscience). γδ T cells were sorted based on scatter ...
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bioRxiv - Immunology 2023Quote: ... at 37 °C for 10 min immediately after the harvest and permeabilized with BD PhosflowTM Perm Buffer III (BD Biosciences) on ice for 30 min ...
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bioRxiv - Physiology 2024Quote: ... Cells were then pelleted at 500 x g for 3 min at 4°C and resuspended in 25 μL of 5 mg/mL FcBlock (rat anti-mouse CD16/32, clone 2.4G2, BD Biosciences) diluted in FACS Buffer and incubated on ice for 10-15 min ...
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bioRxiv - Cell Biology 2023Quote: ... Primary or conjugated antibodies were incubated with the cells overnight at 4°C in Perm/Wash buffer (554714, BD Biosciences) containing 4% FBS and then secondary antibodies for 45 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... The fixed cells were resuspended in PBS and stored at 4°C until the analysis with Flow cytometer Aria III (BD).
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bioRxiv - Molecular Biology 2024Quote: ... The cells were then incubated overnight at 4 °C with primary antibody against TIM23 (1:200; BD-611222; BD Biosciences) diluted in PBS supplemented with 5% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were then incubated overnight at 4 °C with primary antibody against TIM23 (1:200; BD-611222; BD Biosciences) diluted in PBS supplemented with 5% FBS ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were stored in PBS at 4°C for up to 1 week in dark before flow cytometry analysis by LSRFortessa (BD). The data were analyzed by the WEASEL software (2.7 ...
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bioRxiv - Immunology 2024Quote: ... cell suspensions were surface labelled for 30 min at 4°C with CD3 BV785 (clone 17A2, 1:50, BD Horizon), CD8α BUV805 (clone 53-6.7 ...
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Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: ... Cells were incubated with the respective antibodies overnight at 4°C for intracellular labelling and washed with perm buffer (BD). Fluorescence data were acquired on a Cytek Aurora using SpectroFlo v2 Software ...
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bioRxiv - Microbiology 2024Quote: ... The 96-well plate was then incubated over the course of 72 hours at 37°C under anaerobic conditions (GasPak EZ Anaerobe Gas Generating Pouch System with Indicator, BD) with OD600 readings taken periodically over 72 hours with a microplate reader (Varikoskan LUX 3020-1160) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% bovine serum antigen) and stained with either 1:20 anti-HLA-A,B,C-PE (clone W6/32, BD Biosciences), 1:100 anti-β2m-FITC (clone 2M2 ...
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bioRxiv - Cancer Biology 2021Quote: ... for 30 minutes at 4°C and consecutively sorted using a SONY SH800S or a FACS Aria Cell Sorter (BD Biosciences) into NCAM1- and NCAM+ populations ...
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bioRxiv - Cancer Biology 2021Quote: ... for 30 minutes at 4°C and sorted into 384-wells SORTseq plates using a FACS Aria Cell Sorter (BD Biosciences) and directly stored at −80°C until further processing.
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bioRxiv - Microbiology 2020Quote: ... washed with cold PBS by centrifugation (5 min, 550g at 4°C) and analysed with a FACS BD AccuriTM C6 (BD Biosciences) instrument ...
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bioRxiv - Microbiology 2020Quote: ... cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Pacific Blue; BD Biosciences), anti-CD4 (L200 ...
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bioRxiv - Immunology 2021Quote: ... for 5 minutes prior to staining at 4°C in the dark using the antibodies listed in supplementary table 1 and analysed using an LSR Fortessa cytometer (BD Biosciences) and FlowJo software (BD) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were incubated for 30 min in the dark at 4°C after adding relevant antibodies (BD biosciences, Franklin Lakes, NJ). A FACSCalibur system (BD biosciences ...
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bioRxiv - Immunology 2021Quote: ... and surface stained for 30 mins at 4°C with 50 μL/well anti-mouse CD3 APC-CY7 (clone 17A2, 1:400, BD Biosciences), anti-mouse CD4 BV510 (clone RM4-5 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice and incubated overnight at 4°C with the fluorochrome-conjugated antibodies and unconjugated rat and mouse IgG in Perm/Wash solution (BD Biosciences), which was followed by additional 5 min incubation in Perm/Wash solution without antibodies ...
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bioRxiv - Cell Biology 2022Quote: Cells harvested at different time points of reprogramming were stained in FACS buffer for 30 min at 4°C and washed with FACS buffer prior to acquisition with LSR Fortessa (BD Biosciences) cytometer ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... and with hematopoietic stem cell markers APC-conjugated anti-CD117 (c-Kit) and PE-conjugated Ly6A/E (Sca-1) (BD Biosciences). Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C with the astrocyte (mouse anti-GFAP; 1:1000, BD Biosciences, San Diego, CA, USA) and microglia (rat anti-CD11b ...
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bioRxiv - Molecular Biology 2020Quote: ... High sensitivity C-reactive protein (CRP) was measured in serum: blood was collected into a silica and gel containing tube (BD Vacutainer).
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in cold phosphate-buffered saline (PBS) containing 3% FBS and stored at 4°C until analysis using a LSRII flow cytometer (BD Biosciences). FlowJo software v10 (Treestar ...
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bioRxiv - Cell Biology 2022Quote: ... was directly added to the cells and incubated for 30 mins at 37°C and analyzed using a Fortessa flow cytometer (BD Biosciences) at the Imaging and Flow cytometry Core ...
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bioRxiv - Epidemiology 2019Quote: 16N203 was streaked onto TCCFA and an isolated colony was incubated overnight anaerobically at 37°C in 2mL Columbia broth (BD 294420). The next day the culture was placed into 40mL of Clospore (33 ...
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bioRxiv - Immunology 2019Quote: ... Cells were stained for 30 min at 37°C in 1 μM JC-1 dye and analyzed on an LSR Fortessa X20 (BD Biosciences). Aggregates were measured under Texas Red (610/20 600LP ...
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bioRxiv - Immunology 2019Quote: ... We then double sorted lineage depleted cells for an HSC-enriched population (Lin- Sca-1+c-Kit+CD34+-CD135-) into 2% FBS in HBSS using fluorescent activated cell sorting (FACS) (BD Aria). Recipient mice ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were incubated with TrypLE Express for 5 min at 37 °C to generate single cell suspensions and strained through a 40 μm mesh (BD Biosciences). They were then fixed and permeabilized using True-Nuclear Transcription Factor Buffer Set (Biolegend ...
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bioRxiv - Microbiology 2019Quote: ... transduced cells were sorted for single positive mCherry signal or double positive mCherry/eGFP signal at 4°C using FACS Aria III and the corresponding FACS Diva software (BD Bioscience). Cells were sorted from HBSS supplemented with 10 µM Y-27632 ...
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bioRxiv - Genomics 2021Quote: ... tissues were blocked (3% bovine serum albumin in 5% normal goat sera and 0.1% Triton) and incubated overnight at 4°C with primary antibodies: CD31 (Neomarkers, RB-10333-P0) or CDH1 (BD Biosciences, 6315829). Slides were washed and incubated with Cy3-goat-anti-mouse or anti-rabbit-conjugated secondary antibodies (Jackson Immunoresearch Laboratories ...
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bioRxiv - Immunology 2021Quote: Cultured T cells were stimulated with the cognate SARS-CoV-2 OPPs (ORF1ab-C, ORF3a, N, S, nsp12) and incubated for 4 hours in the presence of Golgiplug (BD Biosciences). Following stimulation ...
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bioRxiv - Cancer Biology 2020Quote: Tumor cells were implanted subcutaneously in the flank of BALB/c mice using 5 × 106 cells (WEHI-164) with 0.5 ml 29G insulin syringes (MicroFine™+, BD medical). Tumor volume was measured with a caliper and volume was calculated using the formula ...
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bioRxiv - Immunology 2021Quote: ... and stained for 20 minutes at 4°C with 50 μl of antibody cocktail containing subset-specific antibodies (Supplementary Table 2) diluted in Brilliant Stain Buffer (BD Biosciences). Cells were washed in FACS buffer and fixed for 20 minutes at 4°C in 100 μl of Fixation Buffer (BioLegend).
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bioRxiv - Microbiology 2021Quote: ... neoformans strains were grown at 30°C in yeast extract-peptone-dextrose (YPD) agar or liquid medium (BD Biosciences, Sparks, MD). S ...
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bioRxiv - Bioengineering 2020Quote: Cell surface staining was performed at 4 °C for 30 min and was analysed using a FACSCalibur flow cytometer (BD Biosciences) and CellQuest Software (BD Biosciences) ...
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bioRxiv - Bioengineering 2020Quote: Cell-surface staining was performed for 45 min at 4 °C and was analyzed using a FACSCalibur flow cytometer (BD Biosciences). A minimum of 1 × 104 events per sample were examined.
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bioRxiv - Neuroscience 2020Quote: ... The pellet was recovered and sequentially incubated with anti-mouse CD16/CD32 (1:250, BD Pharmingen; for 15min at 4°C) followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200, BD Pharmingen; for 30min at 4°C), all in 2% BSA ...