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Citations for Becton, Dickinson and Company :
1451 - 1500 of 1656 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The lung infiltrated cells were cultured with purified RBD protein at 10 μg/ml for 12 h at 37 °C followed by a 6 h treatment with GolgiPlug (BD 555028). After blocking with FcγIII and FcγII receptors antibody (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... lung T cells were incubated at 37°C for 5 hours with 5 μM Bl-Eng2 or calnexin peptide and 1μg anti-mouse CD28 (BD, cat 553294). After 1h ...
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bioRxiv - Microbiology 2020Quote: ... Footpads were digested with 4 mg ml−1 collagenase D for 2 h at 37°C before dissociation through 70 μm cell strainers (BD Biosciences). Homogenates were resuspended in HOMEM supplemented with 20% FCS and serial dilutions (2-fold ...
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bioRxiv - Microbiology 2020Quote: ... cell debris pelleted by centrifugation (1400 x g for 10 min at 4°C) and supernatants assayed for chemokine and cytokine levels using the CBA flex set (BD Bioscience) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Cells were stored overnight at 4°C and were then acquired on a Symphony flow cytometer with Diva software (BD Biosciences). A minimum of 5 × 104 events were acquired for each sample.
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli strains were routinely grown aerobically at 37 °C under aerobic conditions with shaking at 1 × g in Luria-Bertani medium (BD, USA), supplemented with 25 μg mL−1 chloramphenicol (Merck ...
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bioRxiv - Immunology 2020Quote: Cell-free supernatants were stored at –20°C until their analysis for chemokine/cytokine content with a multiplexed bead-based immunoassay (BD(tm) Cytometric Bead Array) ...
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bioRxiv - Immunology 2021Quote: ... lung T cells were incubated at 37°C for 5 hours with 5 µM Bl-Eng2 peptide and 1µg antimouse CD28 (BD, cat 553294). After 1h ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged and re-suspended in PBS and stored at 4°C prior to cytometric analysis using a using LSRFortessa flow analyzer (BD Biosciences). Results were analyzed using FlowJo V10 software (Flowjo ...
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bioRxiv - Immunology 2022Quote: ... The tube was then stored in the dark at 4° C for up to 2 hours prior to acquisition on the LSRII or FACSLyric analyzers (BD Biosciences).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated for 15 min at 25 °C in the dark and then analyzed using flow cytometry with a BD FACSCanto II device (BD Biosciences). All data are shown as mean ± standard deviation and are representative of three independent experiments ...
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bioRxiv - Immunology 2022Quote: Splenocytes from infected mice were incubated for 5 hours at 37°C 5%CO2 in cRPMI prior intra-cytoplasmic detection of active-caspase 3 (BD Bioscience) using BD Fixation/Permeabilization kit (BD Bioscience) ...
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bioRxiv - Immunology 2022Quote: ... were re-stimulated in vitro for 5h at 37°C 5% CO2 in cRPMI supplemented with GolgiStop (1/250; BD bioscience), GolgiPlug (1/500 ...
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bioRxiv - Immunology 2022Quote: ... stained on ice for 30’ with relevant cocktails of antibodies and populations of interest were sorted at 4°C on an BD FACSARIA (BD Bioscience) using a 70 μM nozzle in 50% FBS RPMI ...
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bioRxiv - Immunology 2022Quote: ... intracellular cytokine staining was performed at 4°C for 30 minutes using a cocktail of antibodies resuspended in 1x Perm/Wash buffer (BD Biosciences); intracellular antibodies included IFNγ APC-Cy7 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... cultured splenocytes were resuspended in 40 μL FACS buffer containing fluorochrome-conjugated antibodies and incubated 1 hr at 4 °C followed by cell fixation using Cytofix/Cytoperm buffer (BD Bioscience) for 20 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD8 APC (1:100, catalog no. 553035; BD Biosciences); rat anti-CD45 PE (1:100 ...
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bioRxiv - Immunology 2022Quote: ... 20 μm paraformaldehyde fixed lung sections from Mtb-mCherry infected I-Tomcat Ai6 and Sp140−/− I-Tomcat Ai6 mice were stained at 4°C overnight with BV421-labeled SIRP□ (P84, BD Biosciences), Pacific Blue–labeled B220 (RA3-6B2 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then resuspended in FACS buffer (1% BSA in D-PBS) and kept at 4°C in dark until analyzed with a customized BD LSR Fortessa flow cytometer (BD Biosciences). Compensation was performed using ArC and UltraComp compensation beads (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... thermoglucosidasius DSM2542T was routinely grown on 2-SPY medium106 at 60 °C with 150 RPM shaking or on Tryptic Soy Agar plates (BD Biosciences) at 60 °C ...
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bioRxiv - Immunology 2022Quote: ... pErk1/2T202/Y204 and pAktS473 of activated CD4+ T cells was performed by fixing for 15 minutes at 37 °C with BD Cytofix (BD Biosciences), permeabilization for 30min on ice with BD Phosflow™ Perm Buffer III (BD Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... and stained with conjugated antibodies for 1 hour on ice: mouse anti-C-peptide antibody conjugated with Alexa Fluor 647 (BD Biosciences) and mouse anti-glucagon antibody conjugated with Bv421 (BD Biosciences) ...
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bioRxiv - Pathology 2023Quote: ... then incubated for 20 minutes at 4°C with a combination of the following mouse anti-human antibodies: AF488 anti-CD3 (clone UCHT1, BD Biosciences), PE anti-CD203c (clone NP4D6 ...
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bioRxiv - Microbiology 2022Quote: ... of anaerobic incubation at 37°C in a GasPak jar with an EZ Anaerobe Container System Sachets with Indicator (BD BBL), multiple colonies from each plate were inoculated into two (three ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: Lymph node single cell suspension of 2.5×106 cells was incubated for 20 min at 4°C with a purified rat IgG2b anti-mouse CD16/CD32 receptor antibody (BD biosciences). Cells were stained with fluorochrome-conjugated antibodies against CD9 ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated with 1 µg/ml SIINFEKL peptides in complete RPMI medium for 1 h at 37°C followed by brefeldin A (Golgiplug; BD Pharmingen) addition and further 5 h incubation ...
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bioRxiv - Genomics 2023Quote: ... for 5 minutes and stained at a dilution of 1:50 with one of two panels of directly conjugated antibodies for 30 minutes at 4 °C: anti-human CD45 (BD 563792), CD204 (Biolegend 371906) ...
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bioRxiv - Immunology 2023Quote: T cells were staining 30 min at 4°C with anti-human CD3-PE (Cyto Matin Gene, CMGCD3-P100) anti-CD3-PerCP (BD, 347344), anti-CD3-FITC (BD ...
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bioRxiv - Cancer Biology 2023Quote: ... admixed with an equal number of HDFs expressing either S301D mutant or wildtype lamin A/C were re-suspended in 70 µl of Matrigel solution (BD Bioscience) and injected intradermally into the left and right side of the mouse back ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation was overnight at 4 degrees C with the following antibodies at 1:100 dilution in PBS + 3% BSA: CD24-FITC (clone ML5, BD Bioscience), CD271-APC (clone C40-1457 ...
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bioRxiv - Microbiology 2023Quote: ... and Sharpe (MRS) plates supplemented with 1.5% agar at 37°C in anaerobic conditions (BD GasPak™ EZ Anaerobe Container System) overnight ...
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bioRxiv - Microbiology 2023Quote: ... Mtb strain H37Rv with plasmid pSMT3[Phsp60/DsRed] [9] was cultured at 37°C in complete Difco Middlebrook 7H9 Broth (BD Biosciences), supplemented with 10% ADC (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... The cells were cultured at 37°C and 5% CO2 for 72 hours and then the dye dilution was analyzed using a FACSVerse flow cytometer (BD Biosciences), and data were analyzed using FlowJo software (Tree Star).
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bioRxiv - Developmental Biology 2023Quote: Hematopoietic cells were stained with the antibodies listed in Supp Table S1 for 20-30 min at 4°C and analyzed on a custom BD LSR Fortessa (BD Biosciences). For sorting ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 300 µg/mL DNase for 1 hour at 37°C to expose incorporated brdU and then stained with an APC anti-brdU antibody (1:50, BD Biosciences). 1X BD Perm/Wash in ddH2O was used as a staining and washing buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... for 30 minutes at 37°C and the single cell suspensions were filtered through a 70 µm cell strainer (BD Biosciences).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated for 30 minutes at 37°C washed and resuspended in ALDEFLUOR™ assay buffer and rapidly sorted on a FACSAria III cell sorting system (BD). 5×104 freshly sorted ALDHhi cells were seeded in seahorse XF24 plate in growth media and incubated overnight before seahorse analysis as described in analysis of extracellular flux section.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then fixed (eBioscience #00-8222-49) at 4 °C for 20 minutes and permeabilization (eBioscience #00-8333-56) before staining for intracellular IFNγ (BV650, BD #563854) and TNFα (BV421 ...
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bioRxiv - Microbiology 2023Quote: Stained cells were stored for up to 2 days at 4°C in the dark (TRAIL-R1) and flow cytometry was performed on a BD FACSCanto II (BD Biosciences). Raw data were exported to FlowJo v10.8.1 for analysis and plotting.
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then fixed in 2% formaldehyde and stored at 4°C until flow cytometry on an LSR II flow cytometer (BD Biosciences). Cell surface markers included CD3 (clone 17A2 ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated at 4°C for 30 min with APC-conjugated rat anti-mouse CD31 (1:200, BD Pharmigen, 551262). DAPI (5 mg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... was grown overnight (37°C, 4% H2, 5% CO2, 91% N2, <10 ppm O2) in Chopped Meat Carbohydrate Broth (BD297307, BD, USA). Genomic DNA was extracted from B ...
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bioRxiv - Immunology 2023Quote: ... T cells isolated from lymph nodes or tumors were mixed with 3×106 congenically marked B6 splenocytes and incubated with 1 μg/mL of OVA peptide and/or 2 μg/mL of GP peptide for 4-5h at 37°C in the presence of GolgiPlug (BD Biosciences). After staining for cell surface molecules ...
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bioRxiv - Cancer Biology 2023Quote: ... the pLCOs in suspension were centrifuged at 500 × g for 5 minutes at 4 °C and then resuspended in cold growth factor-reduced Matrigel (BD Biosciences). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were incubated overnight at 4°C in the dark and sample analysis performed the following day using an LSR FortessaTM X-20 device (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: Bacteria used in this work are listed in Supplementary Table 7 and were grown at 37 °C in brain and heart infusion (BHI) broth (with agitation) and agar media (BD Difco). Cell lines of human origin used in this work included JEG-3 trophoblasts (ATCC HTB-36) ...
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bioRxiv - Immunology 2023Quote: ... according to manufacturer instructions and stored at 4°C protected from light before acquisition on a FACSCalibur cytometer equipped with CellQuest Pro software (BD Biosciences) within 1 week of fixation ...
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bioRxiv - Genetics 2023Quote: ... 1.106 cells were incubated 20 min at 37°C in 100 μL solution BD cytofix/ cytoperm (BD PharmingenTM BrdU Flow Kit, 552598, BD Bioscience), washed with PBS and re-suspended in 700 µl of staining buffer (PBS with 3% SVF) ...