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Citations for Becton, Dickinson and Company :
1501 - 1550 of 1656 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and ionomycin (500 ng/mL) for 3 hrs at 37°C prior to surface and intracellular staining using the BD Cytofix/Cytoperm Kit (BD Biosciences). Intracellular transcription factor staining was performed using a Foxp3 staining kit (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained with the corresponding FACS panels (Extended Data Table 5) at 4 °C for 30 minutes and sorted using BD Aria III (BD Biosciences) or MoFlo Astrios EQ (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... The tube was then stored in the dark at 4°C for up to 2 hours prior to acquisition on a LSRII or FACSLyric analyzer (BD Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... E8.5 embryos were fixed with 4% paraformaldehyde (PFA) in PBS at 4°C for 3 h and incubated with FITC-conjugated anti-EEA1 (1:100, BD Bioscience) or Cy3-labeled anti-mouse IgG (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... fragilis NCTC 9343 ΔPSA44 (lacks PSA biosynthesis locus) maintained in 25% glycerol at −80 °C were thawed on Brain Heart Infusion agar plates (BHI, BD BBLTM) supplemented with 5 µg/ml hemin (Alfa Aesar ...
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bioRxiv - Immunology 2023Quote: Groups: non-pregnant and pregnant E6-E8 BALB/c background Foxp3IRES-GFP females were used to isolate by FACS (BD FACSAria) several cell populations ...
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bioRxiv - Developmental Biology 2024Quote: All cell culture of all species was maintained at 37°C with 5% CO2 and 5% O2 on Matrigel (1:100, BD Corning) coated plates unless otherwise specified ...
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bioRxiv - Microbiology 2024Quote: ... pyogenes M1T1 5448 strain (56) were grown aerobically at 37°C in Todd-Hewitt Broth supplemented with 2% yeast extract (THY, BD, France). Liquid and solid cultures of L ...
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bioRxiv - Immunology 2023Quote: ... 0.25X and 4X concentration of CD4 T cell-depleting antibody for 30 minutes at 37°C before surface staining with CD3 (clone SP34-2, BD Pharmingen), CD4 (Clone L200 ...
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bioRxiv - Bioengineering 2023Quote: ... The cell suspension was incubated with the following antibodies for 30 minutes at 4 °C: CD106-PE (VCAM-1, 555647, BD Biosciences), CD62E-FITC (E-Selectin ...
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bioRxiv - Cancer Biology 2023Quote: ... per well of U-bottom plate and coincubated for 4h at 37°C in the presence of NH4Cl and 1 µl Golgi PLUG (BD Biosciences). After that time cells were washed three times with 200 µl PBS and centrifuged (5 min ...
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bioRxiv - Bioengineering 2023Quote: ... cerevisiae strains were derived from CEN.PK113-7D and grown at 30°C in Yeast Extract Phytone Dextrose media (YEPhD, 2% Difco™ phytone peptone (Becton-Dickinson (BD), Franklin Lakes ...
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bioRxiv - Bioengineering 2024Quote: ... Cells incubated with IL-10 at 37 °C were fixed and permeabilized for 20 min on ice with Cytofix/Cytoperm (BD Biosciences). Cells were washed twice in permeabilization buffer and further stained with anti-mouse IL-10 in permeabilization buffer for 30 min at on ice ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed with staining buffer, fixed, and permeabilized (20 min, 4°C, dark) using Fixation and Permeabilization Solution (BD Biosciences). Cells were then stained with S100A9 (Clone ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The activated Ramos cells were incubated overnight (18 h) in 5% CO2 at 37°C followed by staining for flow cytometry (BD LSR2) and analysis (FlowJo and Graphpad Prism).
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bioRxiv - Microbiology 2024Quote: ... A sample from the tetrathionate broth was streaked onto MacConkey and XLT4 agar and incubated at 37°C for 48h and inoculated into Rappaport-Vassiliadis (RV) broth (BD DifcoTM) and incubated at 42°C for 24 hours ...
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bioRxiv - Microbiology 2024Quote: All strains were streaked from −80°C cryostocks onto brain heart infusion (BHI) agar (BD, cat. No. 241830, Franklin Lakes, NJ), followed by incubating at 30°C for 24 h ...
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bioRxiv - Microbiology 2024Quote: ... Colonies isolated from growth on Columbia sheep blood agar plates were used to inoculate mNYC III media and cultures were incubated for 48 hours at 37°C under anaerobic conditions (GasPak™ EZ Anaerobe Container System Sachets, BD).
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bioRxiv - Microbiology 2024Quote: ... and NR021 were cultured on Columbia sheep blood agar plates for 48 hours at 37°C under anaerobic conditions (GasPak™ EZ Anaerobe Gas Generating Pouch System with Indicator, BD) from isolates stored at -80°C ...
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bioRxiv - Molecular Biology 2024Quote: ... in PBS at 37°C for 30 min and analyzed with a FACSCalibur or a LSRFortessa X-20 machines (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... surface antibodies were incubated with cells for 30 minutes at 4°C in hFACS buffer and Brilliant Stain Buffer (BD Biosciences). For intracellular staining ...
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bioRxiv - Microbiology 2023Quote: Giardia lamblia trophozoites (strain WBC6, ATCC 50803) were grown at 37°C without shaking in 16-ml screw-cap plastic tubes (BD Falcon) containing 12 ml modified TYI-S-33 medium supplemented with bovine bile ...
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bioRxiv - Cell Biology 2023Quote: ... 0.25% Triton PBS before incubation overnight at 4°C with the following antibodies: Rat monoclonal to mouse Sca1 (740450, BD Biosciences), Rabbit monoclonal to mouse SOX9 (ab185230 ...
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bioRxiv - Immunology 2021Quote: ... Slides were treated for 10 minutes in H2O2 and after blocking in 10% goat serum in 0.1% Tween20 for 30 minutes and incubated overnight at 4°C with specific antibody diluted in PBS 0,1%Tween20: anti-mouse Ly6G (1A8, BD Pharmingen, cat 551459), or anti-mouse Ki67 (D3B5 ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... which was incubated for 2 h in the dark at 37°C with 20 µM caspase-3 substrate Ac-DEVD-AMC (BD Biosciences Pharmingen) or 2 ml from a stock of 1 mg/ml for a final volume of 150 µl ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hr at room temperature and incubated overnight at 4° C with primary antibodies against Syn1 (mouse, 1:1000, BD Biosciences, #610786), pSer129 (rabbit ...
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bioRxiv - Systems Biology 2019Quote: ... Membranes were incubated with primary antibody solutions (using 5% milk or BSA in TBST) overnight at 4°C at the respective dilutions: E-Cadherin (BD, #610181,1:5000), Vimentin (Abcam ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... Cells were then incubated for 1h at RT (or left at 4°C overnight) in the Iridium-intercalator solution in fixation and permeabilization buffer (BD Cytofix/Cytoperm™, BD Biosciences). Then cells were washed with 1x permeabilization buffer (BD Perm/Wash™ ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4 μL of the saturated culture were transferred in 60 uL PBS and the samples were stored at 4 °C for 1-2 days before being assayed by flow cytometry (BD FACSCanto II). Data were analyzed in FlowJo ...
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bioRxiv - Immunology 2021Quote: For TCR stimulation naïve and primed CD8+ T cells were incubated for 20 min at 4°C with biotinylated anti-CD3ε mAb (5 and 1 μg/mL, BD Biosciences #553060). Cells were washed and stimulated for the indicated time by adding streptavidin (20 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed once with wash buffer 1 and incubated for two days at 4°C with mouse anti-SR antibody (1:1,000; BD Biosciences; cat #612052). Sections were washed with wash buffer 2 (2% BSA ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for 1 hour at 4 °C with 2 µl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Bioengineering 2020Quote: ... was applied for 30 minutes at 4°C and stained cells were resuspended in FACS buffer before flow analysis on a BD FACSCanto™ II (BD Biosciences). Gating and flow data were processed on FlowJo (FlowJo LLC).
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bioRxiv - Microbiology 2020Quote: ... The plate was incubated 6 h at 37°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were labeled with murine antibodies against CD3 (BV650) ...
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bioRxiv - Cancer Biology 2020Quote: ... then probed with the following primary antibodies in 5% milk/TBS-T overnight at 4 °C: SREBP-2 (1:250; BD Biosciences, 557037), p44/42 MAPK (ERK1/2 ...
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bioRxiv - Cell Biology 2022Quote: ... washed three times with RPMI and incubated for 30 mins at 37°C in RPMI containing Dispase (BD Cornig, 1:10 dilution) and 30 μg/μl DNase I with continuous and gentle agitation on a rotating shaker ...
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bioRxiv - Cancer Biology 2022Quote: ... coverslips were also stained with mouse anti-ssDNA (DSHB University of Iowa) for 75 minutes at 37°C and detected with goat anti-mouse BV480 (BD Biosciences, 564877) for 45 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... (CGSC#7636) (Baba et al., 2006; Datsenko and Wanner, 2000) were grown at 37 °C in Luria-Bertani (LB) broth (BD, Sparks, MD). E ...
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bioRxiv - Cancer Biology 2021Quote: ... and stained for 1 hour at 4 °C with 2 μl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Immunology 2022Quote: ... 1x105 tumor cells and 1x105 γδ T cells were co-cultured for 3 h at 37°C in the presence of 1 µl α-CD107a-PE antibody (BD Biosciences). Medium or stimulation with α-CD3 and α-CD28 (both 1 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... at 4°C for 5 min before surface staining with a cocktail of the following antibodies for 20 min at 4°C: CD3 V450 (Clone 17A2) (BD Cat. 561389), CD4 BV750 (Clone H129.19 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies (Claudin-5, Abcam Ab131259, 1:1000; Collagen IV, Abcam Ab6586, 1:500; Hemoglobin, R&D Systems G-134-C, 1:300; P62, BD Biosciences 610833 ...
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bioRxiv - Microbiology 2019Quote: ... Pure bacterial cultures were revived from −80°C in tryptic soy broth plus 0.6% yeast extract (TSBYE, Bacto™, BD, Spark, USA). Cultures were plated onto TSAYE agar (TSBYE plus 1.5% agar (Bacto ...
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bioRxiv - Immunology 2019Quote: ... slides were incubated for 50 min at 25 °C with different combinations of the following anti-mouse Abs (Thermo Fisher Scientific, Biolegend, BD Biosciences): Alexa Fluor 488-labeled anti-CD3 (HM3420 ...
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bioRxiv - Immunology 2019Quote: Cells were harvested by centrifugation at 400 g for 5 minutes at 4°C followed by staining with fixable viability stain (BD Horizon FVS700) for 10 minutes on ice in PBS ...
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bioRxiv - Microbiology 2021Quote: ... All strains were grown aerobically on an orbital shaker (150 rpm) at 37 °C in Luria-Bertani (LB) broth (BD Difco, US) overnight ...
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bioRxiv - Microbiology 2021Quote: ... The cMRS agar plates were incubated at 30 °C under aerobic or anaerobic conditions (BD BBL GasPak system (BD, Franklin Lakes, NJ) for 48 h ...
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bioRxiv - Immunology 2019Quote: ... Unfractionated and lineage-depleted (Lin-) cells were labelled with phycoerythrin (PE) conjugated antibodies for CD11b and c-Kit (BD Biosciences, Mississauga, ON), and analyzed by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... The plate was incubated 6 h at 37°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences) for the last 4 h of incubation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... reduced to a single-cell suspension and seeded into a 96-well plate at a density ranging between 1000 – 20 c/w in mTesRTM1 supplemented with 1 nM ROCK inhibitor (BD Biosciences, 562822). Alternatively ...