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Citations for Becton, Dickinson and Company :
1501 - 1550 of 1697 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Total and surface protein expression were measured using the BD Fixation/Permeabilization Solution Kit (BD Biosciences) in conjunction with anti-myc-Alexa 647 staining (clone 9B11 ...
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bioRxiv - Immunology 2020Quote: ... BD GolgiPlug™ Protein Transport Inhibitor (brefeldin A) and BD Perm/Wash™ Buffer from BD Bioscience ...
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bioRxiv - Microbiology 2022Quote: ... inulin (Orafti HP; from Beneo-Orafti, Oreye, Belgium) and protein (Bacto Casitone, BD, Franklin Lakes, NJ); a negative control (stool in basal medium ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining of phosphorylated proteins was performed with Lyse/Fix and Perm III buffers (BD Biosciences). Phosphorylated proteins were then stained during 40 minutes at RT ...
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bioRxiv - Immunology 2021Quote: ... Protein transport inhibitor cocktail and Mouse TNF-α (560478) ELISA kit were procured from BD (USA). The following ELISA kits were brought from R&D ...
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bioRxiv - Microbiology 2022Quote: ... and stained with the antibodies against the following mouse proteins: SiglecF (clone E50-2440; BD Pharmingen), Ly6G (clone 1A8 ...
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bioRxiv - Neuroscience 2023Quote: ... protein transport inhibitors 2μl/mL GolgiPlug (Brefeldin A) and 1.3μl/mL GolgiStop (Monensin) (BD, Biosciences, USA) was added to tubes and further incubated for 3 hours at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Binding to P-selectin was measured by incubating with P-selectin IgG Fusion protein (BD Bioscience), washed and detected with fluorochrome conjugated secondary goat anti-human antibodies (Jackson ImmunoResearch) ...
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bioRxiv - Immunology 2023Quote: ... protein transport inhibitors 2μl/mL GolgiPlug (Brefeldin A) and 1.3μl/mL GolgiStop (Monensin) (BD, Biosciences, USA) were added to the tubes for 8 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The expression level of the surface S proteins was detected by FACS Canto II (BD Biosciences) and analyzed by FlowJo software v10.7.1 (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% penicillin/streptomycin and 10 mM HEPES [pH7.4]) with 25% high protein Matrigel (354248; BD Biosciences). PDX single cell suspensions were obtained by mechanically dissociated (12-141-363 ...
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bioRxiv - Neuroscience 2023Quote: ... astrocytes (1×106) were incubated with protein transport inhibitor containing Brefeldin A (555029 Golgiplug, BD Biosciences) and Monensin (554724 Golgistop ...
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bioRxiv - Immunology 2024Quote: ... surface TCR expression and Nr4a3 Tocky reporter protein expression on an LSR-Fortessa-X20 (BD Biosciences). The following antibodies were utilised in the study ...
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bioRxiv - Immunology 2024Quote: ... Fluorescent protein-expressing populations were then enriched for through FACS using the FACSAriaTM cell sorter (BD).
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bioRxiv - Immunology 2024Quote: ... Five hours before staining for flow cytometry cells were exposed to 1X protein transport inhibitors (BD) to block cytokine release.
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bioRxiv - Cancer Biology 2024Quote: ... Cells expressing high levels of fluorescent proteins were sorted using flow cytometry (BD Influx cell sorter).
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bioRxiv - Cancer Biology 2024Quote: ... The flow cytometry bead array was performed with quantification of proteins using LSRFortessa flow cytometer (BD) to collect 5000 events per sample ...
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bioRxiv - Cancer Biology 2021Quote: Cells from the cell line U3065 obtained from the Human Glioma Cell Culture (HGCC) resource [35] were suspended in stem cell medium and plated on 384 well plates (BD Falcon Optilux TC #353962) coated in laminin ...
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bioRxiv - Cancer Biology 2020Quote: ... Enumeration of human CD45+ cells in spleen and bone marrow was performed by flow cytometry after staining with BV605-conjugated human-specific CD45 antibody (clone HI30, BD Biosciences, San Jose, CA) using a BD LSRII flow cytometer (BD Biosciences ...
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bioRxiv - Bioengineering 2020Quote: ... Coated beads were then incubated with anti-human CD63 (eBioscience, San Diego, CA) and analysed with a BD AccuriTM C6 (BD Biosciences, San Jose, CA).
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bioRxiv - Immunology 2022Quote: ... and incubated for 30 min at 4°C with a cocktail of mouse anti-human antibodies: CD19 Alexa 700 (HIB19, BD Biosciences, San Jose, CA), CD21 BV421 (B-ly4 ...
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bioRxiv - Genetics 2020Quote: ... Cells were stimulated at 1×106 cells/ml with 10 μg/mL soluble Leishmania antigen (SLA) plus 1 μg/mL purified NA/LE anti-human CD28 (clone CD28.2, BD Biosciences, San Jose, CA, USA) at 37°C / 5% CO2 for 15 h and subsequently incubated in the presence of brefeldin A (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... the cells were co-cultured in R10 medium alone or in presence of K562 cells for 2 hours in the presence of anti-human CD107a (FITC or Brilliant Violet 421, H4A3, BD Biosciences, San Jose, Calif.).
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bioRxiv - Biochemistry 2020Quote: ... and TNF in the supernatants was determined by using Cytometric Bead Array (CBA) - Human Inflammatory Cytokines Kit (551811, BD Biosciences, San Jose, CA, USA), that allows the determination of the indicated human cytokines simultaneously ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Whole blood was collected from human volunteer donors with informed consent in 8 to 10 BD Vacutainer CPT 8 ml tubes (BD Biosciences, San Jose, CA). The blood samples were mixed immediately prior to centrifugation by gently inverting tubes 8-10 times ...
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bioRxiv - Immunology 2021Quote: ... harvested and stained for flow cytometry as described (37) using antibodies to human surface antigens: CD4-APCH7 (SK3, BD Biosciences Franklin Lakes, NJ, USA), CD8-PerCP (SK1 ...
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bioRxiv - Immunology 2023Quote: ... a total of 50 ml of whole human blood was collected by a trained phlebotomist from healthy donors in BD Vacutainer® EDTA tubes (BD Vacutainer®, USA). This was previously approved by the RVC’s Ethics and Welfare Committee (URN 2019 1916-3).
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bioRxiv - Molecular Biology 2023Quote: ... Evaluation of proliferation in tissues using antibodies against Ki67 or BrdU (anti-human Ki67 and anti- BrdU monoclonal antibodies; Thermo-Fisher Scientific and BD BioSciences, USA, respectively) was performed as previously described {GarciaMelendrez:GKcY05su } ...
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bioRxiv - Cancer Biology 2023Quote: Ba/F3 cells expressing human MPL protein that were either cultured normally or treated with drugs were harvested and stained for surface MPL using PE-conjugated anti-human MPL antibody (BD Pharmingen 562159,1:50 dilution) in staining buffer for 30 min at 4°C.
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bioRxiv - Immunology 2024Quote: ... blocked with 2% BSA in phosphate buffer pH=7.4 for 30min at RT and incubated with mouse anti-human LAMP1 (BD Biosciences, Franklin Lakes, NJ, USA) for 1h at RT ...
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bioRxiv - Cell Biology 2020Quote: 25 μL protein G Dynabeads were coated with 2 μg of anti-BrdU antibody (#555627, BD Biosciences) for 10 min at room temperature and then blocked for 30 min at room temperature in blocking buffer (0.1% PVP ...
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bioRxiv - Cell Biology 2020Quote: ... The following antibodies were used for detection of the respective proteins: mouse α-Cdc27 (BD Biosciences, #610455), mouse α-cyclin B2 (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2021Quote: ... for IFN-γ assessment in T-cells in the presence of protein transport inhibitor (BD GolgiStop™) for 12 hours ...
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bioRxiv - Immunology 2020Quote: ... followed by fluorochrome-conjugated antibodies for cell surface proteins before fixation and permeabilization (BD Cytofix/Cytoperm Kit). Antibody staining for intracellular cytokines was then performed in permeabilization buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... and 1.5 mg of protein was immunoprecipitated with 2 µg of SRPK1 or SRPK2 antibodies (BD Biosciences). Immunoprecipitated containing beads were then washed with lysis buffer supplemented with 500 mM NaCl and half sample was resuspended in loading buffer ...
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bioRxiv - Immunology 2022Quote: ... and levels of protein expression were analyzed by flow cytometry using a FACS Canto II (BD Biosciences) followed by analysis using FlowJo software (Tree Star).
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bioRxiv - Plant Biology 2020Quote: ... Yeast cells co-expressing proteins fused to the Gal4 activation domain (AD) and DNA binding domain (BD) were selected on medium lacking the amino acids leucine (L ...
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bioRxiv - Immunology 2021Quote: ... Intracellular cytokine staining was performed for macrophage inflammatory protein 1β (MIP-1β; D21-1351, FITC, BD Biosciences) interferon-γ (IFN-γ ...
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bioRxiv - Molecular Biology 2021Quote: ... 750 μgs of protein were incubated with 25 μL of TALON Metal Affinity Bead suspension (BD Biosciences) in a 500 μL final volume for 30 min at 4°C with slow rotation ...
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bioRxiv - Immunology 2020Quote: ... cells were stained for surface proteins if required and then fixed in Cytofix fixation buffer (BD Biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% penicillin–streptomycin and 10 mM HEPES (pH 7.4) with 25% high-protein Matrigel (354248; BD Biosciences)).
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bioRxiv - Physiology 2023Quote: ... livers protein lysates were incubated with fluorescent substrate Ac-DEVD-AMC Caspase-3 Fluorogenic Substrate (BD Biosciences) at 37°C and the reaction was monitored for at least 4 h to track changes in fluorescence (λ excitation of 390 nm and λ emission of 510 nm) ...
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bioRxiv - Microbiology 2023Quote: ... leading to a fusion protein with a GAL4 activation domain (AD) or GAL4 DNA-binding domain (BD) either at the N-or C-terminus ...
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bioRxiv - Immunology 2022Quote: ... The following mAbs were used: anti-CD3ε peridinin chlorophyll protein (PerCP)-Cy5.5 (clone 145-2C11, BD Biosciences), anti-CD8α BUV805 (clone 53-6.7 ...
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bioRxiv - Immunology 2023Quote: ... protein transport inhibitor was prepared according to the manufacturer’s guidelines (RRID:AB_2869014, BD Science, San Jose, CA, USA) and added to all samples ...
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bioRxiv - Neuroscience 2024Quote: ... CD31-CD184+GFAP+ cells as astrocytes or glial cells) and cilia proteins: CD31-BUV396 (BD, cat# 740231), CD184-PerCP Cy5.5 (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... 3) mouse isotype IgG1 anti-C-terminal binding protein 2 (CtBP2, 1:100, BD Transduction Laboratories #612044), 4 ...
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bioRxiv - Genetics 2024Quote: ... which generates a hybrid protein consisting of the GAL4 DNA-binding domain (BD; amino acids 1–147) and an open reading frame cloned into MCS I ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were then stained for 30min at 4°C with the following antibodies: monoclonal anti-human CD33 V450 (WM53; BD Biosciences, San Jose, CA, USA); HLA-ABC FITC (W6/32 ...
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bioRxiv - Biochemistry 2020Quote: ... an affinity-purified mouse mAb that recognizes an epitope in the C-terminal NAD binding and catalytic domain of human PARP1 (clone 7D3-6, BD Biosciences #556493; 1:500 dilution); anti-cc-PARP1 ...