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Citations for Becton, Dickinson and Company :
1301 - 1350 of 1697 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: Meiotic and kinetochore genes tagged with Enhanced Green Fluorescent Protein (EGFP) (from BD Biosciences) were used ...
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bioRxiv - Immunology 2022Quote: ... and BD GolgiPlug Protein Transport Inhibitor (containing brefeldin A; 1 mg/mL; BD Biosciences). Cells were washed ...
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bioRxiv - Immunology 2021Quote: ... and cells were stained for interferon-γ and macrophage inflammatory protein-1β (BD bioscience). Flow cytometry was performed with an IQue (Intellicyt ...
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bioRxiv - Immunology 2020Quote: ... Then, GolgiPlug and GolgiStop (brefeldin A and monensin, respectively) protein transport inhibitors (BD Biosciences) were added ...
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bioRxiv - Immunology 2021Quote: ... The protein transport inhibitor monensin (GolgiStop, Cat#51-2092KZ, BD Biosciences, San Jose, CA) was added to the wells to prevent cytokine secretion 60 minutes after the addition of the peptides ...
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were detected using primary polyclonal anti-FliK and anti-FliC (BD) antibodies as well as monoclonal anti-DnaK (abcam ...
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bioRxiv - Immunology 2021Quote: ... Protein concentration was normalized through the Bradford assay (BD Biosciences, San Diego, CA, USA). The absorbance was taken using Multiskan Go (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and blotted for antibodies against specific proteins HIF1α (BD biosciences, cat# 610958, 1:1000), HK-2 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2022Quote: ... anti-C-terminal binding protein 2 (mouse anti-CtBP2 IgG1; BD Biosciences; 1:200), and anti-glutamate receptor 2 (mouse anti-GluR2 IgG2a ...
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bioRxiv - Immunology 2023Quote: ... The green fluorescent protein (GFP) signal from cells was measured using a FACSVerse (BD). To measure autophagic flux ...
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bioRxiv - Biochemistry 2023Quote: ... cells expressing red fluorescent protein were enriched by FACS Aria Fusion SORP (BD Bioscience) and plated into a dish at a very low density ...
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bioRxiv - Systems Biology 2023Quote: ... All of the other data considered here (MERFISH, BD Rhapsody mRNA and protein, etc.) ultimately consists of a similar matrix ...
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bioRxiv - Cancer Biology 2023Quote: CD81 cell surface protein expression was determined by FCM (LSR Fortessa X20, BD Biosciences), 5 x105 cells were labeled with 1 μg anti-hCD81-PE clone 5A6 or 1 µg isotype control-PE (349506 ...
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bioRxiv - Immunology 2023Quote: ... CD3 peridinin chlorophyll protein (PerCP) Cy5.5 clone SP34-2 (BD Biosciences Cat No. 552852), IgD phycoerythrin (PE ...
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bioRxiv - Immunology 2024Quote: ... in the presence of BD GolgiPlug and BD GolgiStop protein transport inhibitors (BD Biosciences) at 1:1000 and 4:6000 concentration per manufacturer recommendation ...
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bioRxiv - Immunology 2024Quote: ... and Monensin protein transport inhibitors (4 µ L/6E6 cells; GolgiStop, BD Biosciences, 554724) and incubated for 4 hours at 37°C ...
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bioRxiv - Immunology 2024Quote: ... cells were washed and 5 μL of 7AAD-Peridinin-Chlorophyll-Protein reagent (BD Biosciences) was added to the cell suspension and incubated in the dark for 15 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... intracellular proteins were stained with fluorochrome-conjugated antibodies in Perm/Wash buffer (BD Biosciences) for 30 min at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... White cells were resuspended in PBS supplemented with 1% human serum and 2 mM EDTA and analyzed on an LSR Fortessa instrument (BD Biosciences).
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bioRxiv - Immunology 2021Quote: Supernatants were collected 4 or 24 h post-challenge and the levels of TNF and mature IL-1β were quantified by ELISA using OptEIA human TNF ELISA Set (BD Biosciences) and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems) ...
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bioRxiv - Immunology 2021Quote: CD47 expression or cross-dressing on cells was determined by direct staining with BV786-conjugated anti human specific CD47 mAb B6H12 (abbreviated anti-hCD47; BD Bioscience) or PE-conjugated anti-human CD47 mAb CC2C6 (with cross-reactivity to pig CD47 and thus ...
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bioRxiv - Microbiology 2020Quote: Macrophages were infected in a 48-well plate as described above and harvested supernatants were assayed for cytokine levels using ELISA kits for human IL-1β (BD Biosciences) and IL-18 (R&D Systems).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were re-suspended in 100 μl of FCSB and 1 μl of mouse anti-human CD10-PE (561002, BD Biosciences) and mouse anti-human CD92-AF647 (565316 ...
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bioRxiv - Neuroscience 2022Quote: Dissociated and resuspended iTF-Microglia were blocked for 15 min with 1:20 Human FC Block (BD Biosciences; Cat. No. 564220) and then stained with 1:66 PE/Cy7 anti-human CD184 (CXCR4 ...
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bioRxiv - Immunology 2022Quote: ... NK cells were added to the washed ELISA plate and incubated together with anti-human CD107a (BD, 1:40, clone: H4A3), brefeldin A (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... primary hMDMs were transfected in a 48-well plate as described above and harvested supernatants were assayed for cytokine levels using an ELISA kit for human IL-1β (BD Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... Cytokine concentrations in the culture supernatant were measured using BD Cytometric Bead Array Human Th1/Th2 Cytokine Kit II (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Cytokines in the serum sample were measured using human Th1/Th2/Th17 cytometric bead array kit (BD Bioscience, San Jose, CA) according to the manufacture’s instruction ...
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bioRxiv - Immunology 2021Quote: ... labelled with anti-human myeloid and stromal markers (Table 1) and analysed by flow cytometry on a LSR Fortessa (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then washed twice 5 min in Tris buffer saline containing 0.01% Triton X-100 (TBST) and incubated with the primary antibody over night at 4°C (human and mouse a-Syn: 610786 BD Biosciences). After incubation with the first antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Human CD34C-derived ECs were seeded at a density of 8×104/insert onto the Matrigel-coated (BD Biosciences, United States) Transwell inserts (3401-Costar ...
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bioRxiv - Microbiology 2020Quote: ... adjusted to 106 CFU ml-1 in HBSS and 104 CFU (10 μl) used to inoculate 90 μl of freshly donated human blood (collected in EDTA-treated tubes; BD Biosciences) or freshly diluted H2O2 (10 mM in PBS ...
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bioRxiv - Microbiology 2020Quote: ... IL-10 and IL-12p70 were quantified in those culture supernatants by ELISA with BD OptEIA™ human IL-10 and IL-12p70 sets following the manufacturer's instructions (BD Bioscience).
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bioRxiv - Genomics 2021Quote: ... hPBMCs were resuspended in 1x PBS + 2% FBS and stained with a FITC-conjugated Mouse Anti-Human CD45 Antibody (Clone HI30, BD Biosciences) for 25 min on ice ...
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bioRxiv - Immunology 2020Quote: ... 8.0 × 105 cells per condition were fixed in 1% PFA for 10 min at room temperature and subsequently stained with primary antibodies: mouse anti-human PEcy7-CD16 (BD #557744), mouse anti-human PerCPcy5.5-CD66b (Biolegend # 305107) ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular staining of the cytokines was performed after fixation and permeabilization using APC conjugated anti-human IFNγ (BD Bioscience, Cat# 551385), anti-human IL2 (BD Bioscience ...
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bioRxiv - Cancer Biology 2021Quote: ... Flow cytometry for negative checkpoint regulator was performed as previously described(14) using antibodies: BV510 mouse anti-human CD279 (PD1) (1:100; 563076, BD Horizon), PerCP-eFluor 710 mouse anti-human CD223 (LAG-3 ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic stem cell (hESC) line H9 and human induced pluripotent stem cell (hiPSC) line S3 were cultured on Matrigel (BD Biosciences)-coated plates in mTesR1 medium (19 ...
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bioRxiv - Immunology 2022Quote: ... For measurements of CXCL13 the cells were cultured for 5 hours at 37°C in RPMI supplemented with 10% human AB serum and GolgiStop (1/1500; BD Biosciences). For cytokine measurements the cells were plated in the presence of anti-CD3/CD28 (Dynabeads® Human T-activator CD3/CD28 ...
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bioRxiv - Genetics 2022Quote: ... cells were resuspended in 90 μL staining buffer and incubated with 10 μL of anti-human CD21-PE (Cat #555422, BD Bioscience), anti-human CD55-PE (MHCD5504 ...
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bioRxiv - Biochemistry 2022Quote: ... TCR expression on transfected Jurkat cells was assessed by staining with phycoerythrin (PE)-conjugated mouse anti-human CD3 (BD Pharmingen 555333); TCR expression was unaltered by Csk overexpression.
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bioRxiv - Immunology 2020Quote: ... TNF-α and IFN-γ in the serum of experimental monkeys were detected with the Non-Human Primate (NHP) Th1/Th2 Cytokine Kit (BD) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... sequenced reads were aligned to the hg38 human transcriptome and the expression of transcripts in each cell was quantified via the standard Rhapsody analysis pipeline (BD Biosciences) on Seven Bridges (https://www.sevenbridges.com) ...
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bioRxiv - Immunology 2020Quote: PBMC were labelled for FACS staining with mouse anti-human antibodies directed against lineage markers (Vioblue-CD3, CD14, CD19, CD56 and CD11c) (BD Biosciences), APC-Vio770 HLA-DR ...
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bioRxiv - Immunology 2020Quote: ... followed by staining for 3 days at 37 °C with 1:100 dilutions of BV421-labeled mouse anti-human CD35 clone E11 (BD Biosciences) and Alexa Fluor 488-labeled mouse anti-Ki67 clone B56 (BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... to stain for CD69 receptor using 5 µL (100 µg/mL) of PreCP-Cy ™ 5.5 mouse anti-human CD69 (BD Biosciences). Staining was done for 45 mins on ice then washed one time with 2 mL RPMI-1640 medium and reconstituted in 250 µL RPMI-1640 medium ...
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bioRxiv - Neuroscience 2022Quote: ... Syn1 [a mouse monoclonal antibody that recognizes residues 91-99 from the NAC region of human α-synuclein (51)] (BD Biosciences) at 1:4,000 and PER4 at 1:4,000 ...
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bioRxiv - Immunology 2022Quote: ... The membranes were then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk for 2 h ...
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bioRxiv - Immunology 2022Quote: ... and then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk in each channel for 2 h ...