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Citations for Becton, Dickinson and Company :
1401 - 1450 of 1697 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Isolation of CD4+ T cells was carried out by negative selection using the Human CD4 T Lymphocyte Enrichment Set-DM (BD Biosciences). All patient samples were obtained with informed consent for research use ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary AML cells were harvested and stained with human CD45 and cells were analyzed by a high-throughput automated plate reader (BD LSRFortessa). Dead cells were excluded using the Near-IR Live/Dead marker (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: Human and Non-human primate samples were processed similarly: Blood was collected in 8 mL sodium citrate-containing CPT tubes (BD Biosciences) and separated into plasma and peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Immunology 2023Quote: ... BM aspirate samples from heathy donors using a Ficoll density gradient and stained with the following anti-human antibodies: IgD-Brilliant Violet 480 (Cat. No. 566138; BD Biosciences), CD3-BUV737 (Cat ...
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bioRxiv - Immunology 2023Quote: ... Cytokine concentrations in the supernatants of DC cultures were measured using a BD human inflammatory cytometric bead array kit (BD #551811) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cells were then harvested and incubated in a blocking buffer containing 10%FBS and 1μg/mL Human Fc block (BD Biosciences, USA) for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Cells were activated for 3 days with 1 μg/mL/million cells of purified NA/LE mouse anti-human CD3 and CD28 (BD Biosciences) and infected with HIVBaL at 0.01 ng/106 cells ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... and stained for 30 min at 4 °C with the following antibody mixture: PerCP-Cy5.5 mouse anti-human CD195 (CCR5, BD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Fixed cells were stained for 30 min at 4° C with PerCP-Cy5.5 mouse anti-human CD195 (CCR5, BD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... IL-2 levels in the co-culture supernatant were quantified using ELISA (BD OptiEIA Human IL-2 ELISA kit, BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... ∼1.0 × 106 cells treated with or without 200 ng/ml dox were stained with APC Mouse Anti-Human IgM (BD 551062) diluted 1:100 in FACS Buffer (1× PBS with 2% FBS ...
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bioRxiv - Bioengineering 2024Quote: ... was determined using flow cytometry after incubation with a PE-conjugated anti-human CD25 antibody (cat. 555432, BD Biosciences, BD PharMingen).
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bioRxiv - Bioengineering 2024Quote: ... was determined using flow cytometry after incubation with a PE-conjugated anti-human CD25 antibody (cat. 555432, BD Biosciences, BD PharMingen).
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bioRxiv - Bioengineering 2024Quote: ... the cells were incubated with PBS/1% BSA for 30 min at 4 °C for blocking unspecific binding and washed with PBS prior to the incubation with PE-conjugated anti-human CD69 antibody (cat. 555531, BD Biosciences, BD PharMingen) for 30 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed with the kit washing buffer and stained with the intracellular mix containing the following anti-human antibodies: Ki67 (BUV395, 1/160, BD Biosciences) and Foxp3 (AF488 ...
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bioRxiv - Immunology 2024Quote: ... with regular verification of the depletion step by staining the incubated cells with an anti-human IgG fluorescent antibody and running these cells on the LSRII flow cytometer (BD Biosciences) (Figure 1A) ...
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bioRxiv - Immunology 2024Quote: ... Cells were further stained intracellularly with antibodies against anti-human CD3 (APC-Cy7, clone: SP34-2, BD, cat# 557757, 1:200), anti-human IFNγ (AF700 ...
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bioRxiv - Immunology 2024Quote: ... was used to isolate the neutrophil population (verified by flow cytometry using an APC-cy7 antibody specific for human CD16 (clone 3G8, BD Biosciences) and a PE antibody for human CD66b (clone C10F5 ...
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bioRxiv - Immunology 2024Quote: ... and each tube was stained with 5 μL of APC-Cy7 Mouse Anti-Human CD31 (PECAM-1) antibody (clone WM59, BD Biosciences) and 5 μL of APC anti-human CD144 (VE-Cadherin ...
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bioRxiv - Cell Biology 2024Quote: ... Tissue and cell slides were then blocked with 1% BSA and 10% normal goat serum in PBS at room temperature for 1 hour followed by overnight incubation at 4°C with primary antibodies (1:50 dilution) against human HIF1α (cat # 610958, BD Biosciences), rat HIF1α (cat # NB100-105 ...
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bioRxiv - Microbiology 2024Quote: ... the indicated cytokine cytometric beads and PE-conjugated detection antibodies from the BD Cytometric Bead Array (CBA) Human Inflammatory Cytokines Kit (BD Bioscience) were mixed with LPS treated THP-1 supernatants according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... CD81/CD274 expression profiles were measured 5 days post-infection of dual MS2/PP7 sgRNAs following staining with antibodies against both targets (APC Mouse Anti-Human CD81 antibody (Cat# 561958, BD Bioscience) and Alexa Fluor 488 Mouse Anti-Human CD274 antibody (Cat# 53-5983-42 ...
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bioRxiv - Cancer Biology 2024Quote: ... detecting the ratio of human CD45 (labelled with PE) to mouse CD45 (labelled with APC-Cy7) on a LSRFortessa instrument (BD Biosciences). Upon detection of engraftment oral administration of 0.033% or 0.1% VTP50469 (supplemented rodent special diet ...
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bioRxiv - Cell Biology 2024Quote: Human samples were incubated in the dark at room temperature with 1.2 µL of an equal mix of BD Pharmingen™ PE Mouse Anti-Human CD62P (BD Biosciences) and BD™ FITC Mouse Anti-Human PAC-1 (#340507 ...
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bioRxiv - Immunology 2020Quote: ... and proteins surface expression assessed by Accuri C6 Plus flow cytometry (BD Bioscience, Oxford, UK). Cytokines and chemokines were measured in the cell media supernatant by ELISA (Peprotech) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells expressing fluorescence proteins were collected uisng a FACS Aria III flow cytometer (BD Biosciences), and used for downstream experiments.
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bioRxiv - Neuroscience 2021Quote: ... C-terminal-binding protein 2 / RIBEYE ribbon synapse maker (Schmitz et al., 2000) (CtBP2, BD Bioscience ...
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bioRxiv - Immunology 2020Quote: Surface marker proteins and intracellular cytokine responses were quantified via flow cytometry (LSRII, BD Biosciences) and analyzed using FlowJo software version 10.5.3 (TreeStar Inc. ...
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bioRxiv - Immunology 2021Quote: ... Cell surface expression of adhesion molecule protein was measured by multiparameter flow cytometry (BD Fortessa). Supernatants were retained and secreted chemokines were measured by ELISA (IP-10 ...
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bioRxiv - Immunology 2020Quote: ... Expression of proteins was measured using FACS Canto II HTS (BD Biosciences, Bedford, MA, USA) and analyzed using FlowJo software (FlowJo LLC ...
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bioRxiv - Immunology 2020Quote: ... HA proteins were tetramerized through sequential addition of streptavidin-BV650 or streptavidin-BV785 (BD biosciences), with HA in excess to streptavidin ...
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bioRxiv - Microbiology 2021Quote: ... Expression levels of surface S proteins were analyzed using a FACS Canto II (BD Biosciences). RL activity was normalized to the mean fluorescence intensity (MFI ...
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bioRxiv - Plant Biology 2021Quote: WRKY proteins used for this experiment were fused into pGBT9 vector (containing GAL4 DNA-BD) and transformed into yeast strain Y190 ...
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bioRxiv - Immunology 2021Quote: ... protein transport inhibitor (BD Golgi Plug™ containing Brefeldin A, 1 µg/mL and BD Golgi Stop™ containing monensin ...
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bioRxiv - Immunology 2021Quote: ... protein transport inhibitor (BD Golgi Plug™ containing Brefeldin A, 1 µg/mL and BD Golgi Stop™ containing monensin ...
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bioRxiv - Immunology 2023Quote: ... and 1 µL/mL GolgiPlug™ Protein Transport Inhibitor (containing Brefeldin A, BD Biosciences, #555029). Cell suspensions were washed between each step with sterile PBS containing 2% FBS (FACS buffer).
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bioRxiv - Physiology 2023Quote: Protein concentrations of multiple serum cytokines were measured using a CBA Kit (BD Biosciences: 558264) and flow cytometry ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were stained with antibodies specific for secretory proteins: Granzyme B (BD, Cat. # 396407), Interferon-gamma (BD ...
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bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... for 4 h and with (1:1500 dilution) GolgiStop protein transport inhibitor (#554724, BD Biosciences) added for the last 2 h of stimulation prior surface staining ...
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bioRxiv - Immunology 2024Quote: ... 1% Penicillin/Streptomycin and Protein Transport Inhibitor (Containing Brefeldin A) (diluted 1:1000) (BD, 555029). The cells were incubated for four hours at 37°C and further washed with 1x PBS to proceed for intracellular cytokine staining.
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bioRxiv - Immunology 2024Quote: ... and protein transport was blocked for the final 6h with GolgiPlug (1:1,000; BD Biosciences) and GolgiStop (1:1,500 ...
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bioRxiv - Immunology 2024Quote: ... for 4-16 hours at 37C with the protein-transporter inhibitor Golgi Stop (BD Biosciences) added during the last 4 hours of culture ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in 50 µL of FACS buffer (PBS plus 1% BSA) and blocked with µL 1 human Fc block (BD Biosciences, 564219) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Flow cytometry was conducted and the supernatant was collected from each well to assess IFNγ and CCL5 levels using a Human IFNγ ELISA Set (BD OptEIA, 555142) and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... Then cells were blocked by PBS plus 5% BSA and later incubated with FITC Anti-Human CD24 (BD Biosciences, San Jose, CA), and PE Anti-Human CD44 (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... To assess activation marker status T cells were stimulated with 0.1 μg/mL of D117C or PIGF on collagen coated plates for 16-20 hr and surface-stained with anti-human CD3 BUV395 (BD clone SK7), CD4 BV785 (Biolegend clone RPA-T4) ...