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Citations for Becton, Dickinson and Company :
1251 - 1300 of 7577 citations for 2 Bromo 1 5 bromofuran 2 yl ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... approximately 200 μL cells were added into the flow tube containing 2 mL PBS and tested by the flow cytometry (BD, New Jersey, USA). Three independent assays were performed.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed with Hank’s buffered saline solution and resuspended in 2mM EDTA/ 2% BSA/ PBS buffer containing [2µg/mL] propidium iodide (PI) (BD Biosciences, Cat. No. 556364) and [1µM] Calcein violet (Invitrogen ...
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bioRxiv - Immunology 2020Quote: Female C57BL/6J mice aged 8 – 12 weeks were injected intraperitoneally with 2 ml each of 3% sterile thioglycollate (BD Biosciences, catalog # 211716) medium ...
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bioRxiv - Immunology 2023Quote: ... All 5 samples were then pooled and stained for surface markers in 70μl of the antibody cocktail (Supplementary Table 2) in BD Horizon Brilliant Stain Buffer Plus (BD Biosciences, San Jose, CA) for 30 minutes at 4 °C ...
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bioRxiv - Pathology 2023Quote: ... Cells were diluted in basal medium containing 2% dextran-coated charcoal-stripped serum (DCC) (HyClone Laboratories, Logan, UT) and plated into culture dishes (BD Falcon, Bedford, MA). Cells were cultured for 24 hours in an incubator at 37°C with saturating humidity and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences, 554419], anti-IFN-γ [BD Biosciences, 557735], and anti-CD107a [BD Biosciences, 560647]) by 20 minutes of incubation at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... 10 min at room temperature) and the supernatants were collected to measure the levels of IL-2 by ELISA using the OptEIA™ kit (BD Biosciences, USA), according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were diluted in basal medium containing 2% dextran-coated charcoal-stripped serum (DCC) (HyClone Laboratories, Logan, UT) and plated into culture dishes (BD Falcon, Bedford, MA). Cells were cultured for 24 hours in an incubator at 37°C with saturating humidity and 5% CO2.
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bioRxiv - Immunology 2022Quote: ... cells counted under the microscope and stained in FACS buffer (PBS, 2% FCS, 2mM EDTA) with purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Catalog #553141) and the specific antibody cocktail ...
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bioRxiv - Immunology 2022Quote: ... Spleens were disrupted using a syringe in 2% FBS/PBS and passed through a 40 μm nylon cell strainer (BD, catalog no. 352340) to obtain a single-cell suspension ...
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bioRxiv - Bioengineering 2022Quote: ... digested cells were resuspended in FACS buffer (2% FBS–PBS) and analyzed by a FACS Calibur flow cytometer (Becton, Dickinson and Company, USA). Compensation and analysis of results were performed using Flow Jo software.
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed for 10 min in 2% paraformaldehyde and were analyzed using a FACS CANTO II (BD Biosciences, Franklin Lakes, NJ, USA). For lentiviral transduction efficiency assessment ...
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bioRxiv - Cancer Biology 2023Quote: ... of both preparations was determined by titration onto target cell lines and quantification of the percentage of BFP+ cells 2–3 days post-transduction by flow cytometry (BD Biosciences LSR II).
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bioRxiv - Immunology 2023Quote: ... Naïve T cells were resuspended in complete medium (KOHJIN BIO DMEM-H (16003016) + 10% FBS) and activated in the presence of 2 μg/mL of anti-CD3 (BD Pharmingen, REF: 553058) and 2 μg/mL of anti-CD28 (BD Pharmingen ...
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bioRxiv - Neuroscience 2024Quote: ... Cell counting was performed in a hemocytometer and 2 million cells from each spleen were used for immunostaining and analysis in a flow cytometer (BD LSRFortessaTM X-20).
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bioRxiv - Immunology 2024Quote: ... 2×106 cells were stained as described above and sorted using a BD FACSAria II flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA). In the control group ...
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bioRxiv - Neuroscience 2024Quote: ... 250,000-350,000 donor cells or the same volume of empty HBSS+2% FBS vehicle were intraperitoneally (IP) injected using a 30 G insulin syringe (BD Biosciences cat# 328431). Transplanted cells were allowed 2 weeks to engraft prior to SCI.
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bioRxiv - Biochemistry 2024Quote: Wild type RPE1 cells or RanGAP1ΔNES clones 2 and 10 were grown on 10 cm plates to 40-70% confluence and pulsed with 10 µM BrdU (BD Pharmigen flow kit) for 45 minutes ...
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bioRxiv - Immunology 2023Quote: ... PerCP-Cy5.5-conjugated anti-IFN-γ (Clone 4S. B3, 1:10), and PE-cy7-conjugated anti-TNF-α (Clone Mab11, 1:50) (BD Biosciences) for intracellular staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies against IdU and CldU were diluted in 5% BSA at 1:100 (BD Biosciences 347580) and 1:250 (abcam ab6326 ...
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bioRxiv - Systems Biology 2022Quote: ... ~1 × 10^5 events were collected for each sample with a BD LSRFortessa system (BD Biosciences, USA) and FlowJo software 7.6.1 was used for data process.
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bioRxiv - Cell Biology 2020Quote: ... washed 5 times with ice-cold PBS and then resuspended in 1× binding buffer (BD Biosciences, USA) at a concentration of 1 × 106 cells/mL ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.1% Tween 20] and 5% milk for 1 hour and incubated overnight with GTF2I (BD Biosciences) and GAPDH (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were subcutaneously injected using a 1 mL 25G x 5/8 syringe (BD, cat. no. 309626). Mice were visually monitored for tumor formation routinely following injection ...
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bioRxiv - Immunology 2023Quote: ... IL-5 (BD Biosciences) and IL-13 (R&D Systems ...
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bioRxiv - Microbiology 2024Quote: ... enrofloxacin (BD; 5 μg), and ceftiofur (BD ...
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bioRxiv - Developmental Biology 2021Quote: Mouse ESCs were pre-differentiated to an epiblast-like state as previously described [2] by seeding fibronectin-coated dishes (BD Biosciences cat. no. 356008) at a density of 0.8× 104 cells per cm2 in NDiff227 media (Takara cat ...
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bioRxiv - Neuroscience 2020Quote: ... and plated as dissociated cells (25 × 104 cells/well) on day −2 in a 6-well Matrigel-coated plate (BD Biosciences, San Jose, CA). On day −1 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS 2% FBS and analyzed by flow cytometry using a FACSCanto and Novocyte flow cytometer (BD Biosciences and Acea Biosciences). The population of interest was gated according to its FSC/SSC criteria ...
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bioRxiv - Developmental Biology 2020Quote: Cryopreserved cells were thawed at room temperature and washed twice with 10 ml of FACS buffer (PBS and 2% FBS) and filtered through a 100 µM filter (BD BioSciences, cat no: 352360). Cell concentration was determined by hemocytometer ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville MD) and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean of the fluorescence intensity of bead-positive cells and dividing by 10,000.
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bioRxiv - Immunology 2022Quote: ... the cells were fixed with 2% PFA and stored at 4°C until the samples were acquired on a LSRII cytometer (BD Biosciences, Mississauga, ON, Canada). Data analysis was performed using FlowJo v10.5.3 (Tree Star ...
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bioRxiv - Biochemistry 2022Quote: ... either single GFP+ cells (to obtain clones 1 and 2) or 250 GFP+ cells (to obtain the pool) were sorted per well into a 96 well plate (BD FACSAria Fusion Cell Sorter). The two clones and the pool were expanded and the decreased ArgBP2 expression was confirmed by immunoblotting.
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bioRxiv - Molecular Biology 2020Quote: ... cells were fixed using 2% paraformaldehyde (PFA) solution for 30 minutes and permeabilization was performed using a 1X BD perm/wash solution (BD Biosciences, USA; cat#554723) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... the cells were co-cultured in R10 medium alone or in presence of K562 cells for 2 hours in the presence of anti-human CD107a (FITC or Brilliant Violet 421, H4A3, BD Biosciences, San Jose, Calif.).
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used in the cocktail for surface staining: CD3-allophycocyanin (APC)-Cy7 (clone SP34-2, Cat#557757, BD Pharmingen, San Jose, CA), CD4-PE-CF594 (clone L200 ...
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs were assessed to determine the frequency of circulating T follicular helper (Tfh) using a panel which included the following antibodies: CD3 (BD Biosciences; clone SP34-2), CD4 (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: All mycobacterial strains (Supplementary File 2 – Key Reagents) were grown in liquid culture containing Difco™ Middlebrook 7H9 Broth (BD Biosciences, San Jose, CA) and supplemented with 0.2 % (v/v ...
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bioRxiv - Immunology 2020Quote: ... Cells were collected from wells by pipetting with PBS (containing 2%FBS and 2mM EDTA) and data acquisition was performed on a BD Accuri C6 (Becton, Dickinson and Company, USA). All flow cytometry data were analyzed with FlowJo v10.1 (BD Biosciences).
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bioRxiv - Microbiology 2022Quote: ... After 19h incubation at 37°C, the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville, MD) and fluorescence was evaluated on a LSRII (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean fluorescence intensity (MFI ...
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bioRxiv - Bioengineering 2023Quote: ... Fixed and permeabilized cells were washed twice with PBSA buffer and incubated for 2 hr at room temperature with Alexa Fluor 647-conjugated anti-STAT5 pY694 antibody (BD Biosciences, clone 47/Stat5) diluted 1:50 in PBSA buffer ...
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bioRxiv - Immunology 2024Quote: ... blocked with 2% BSA in phosphate buffer pH=7.4 for 30min at RT and incubated with mouse anti-human LAMP1 (BD Biosciences, Franklin Lakes, NJ, USA) for 1h at RT ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial strains were prepared for agar overlay plating by streaking out from glycerol stocks onto 2% streak agar plates (BD Difco 2216 marine broth), and allowed to grow for one days at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Cells were enumerated using a Cellometer X2 cell counter (Nexcelom Bioscience, Lawrence, MA, USA) and resuspended in yeast nitrogen base supplemented with 2% glucose (Difco; BD, Franklin Lakes, NJ, USA), to obtain a concentration of 2.0 × 106 cells/ml ...
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bioRxiv - Cell Biology 2024Quote: ... namely anti-CD3-APC (1:5 dilution, clone SK7) and anti-CD45-PerCP (1:10 dilution, clone 2D1) (both from BD Biosciences, San Jose, CA). After incubation for 30 min at 4°C in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was blocked in 5% non-fat dry milk in TBS/Tw and then incubated overnight with primary antibody Syn-1 (BD Biosciences #610,787, 1:1000) or MJFR-14-6-4-2 (Abcam #209,538 ...
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bioRxiv - Neuroscience 2020Quote: COs were washed 3 times for 5 minutes in 5 mL of PBS in 5 mL round bottom tubes (BD Falcon, #1152367) then fixed overnight in 4% PFA at 4°C on a rocker ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 µg/ml of a NA/LE hamster anti-mouse CD29 clone HM β1-1 (562219; BD Bioscience), and 5 µg/ml of a NA/LE hamster IgG2 (553961 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed and resuspended in PBS/2% FBS for analysis on a five-laser flow cytometer (FACSymphony A3 Cell Analyser, BD Bioscience, San Jose, CA, USA). Data were analyzed with FlowJo software (TreeStar ...
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bioRxiv - Neuroscience 2020Quote: Neurons from 18-day-old rat embryos were dissected and dissociated using trypsin and plated at a density of 70,000 cells cm−2 in minimal essential medium (MEM) supplemented with 10% NU serum (BD Biosciences, Le Pont de Claix, France), 0.45% glucose ...