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Citations for Becton, Dickinson and Company :
1051 - 1100 of 7577 citations for 2 Bromo 1 5 bromofuran 2 yl ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and memory B cells (CD19+CD27+) were sorted from PBMCs of SLE patients used in scRNAseq experiment 2 using a FACS Aria (BD). 0.4-35k cells per population were lysed in RLT buffer (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... Protoplasts were stained with 14 µM 4’,6-diamidino-2-phenylindole (DAPI) in PBS and the DAPI negative cells were sorted by BD FACSAria II ...
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bioRxiv - Immunology 2024Quote: ... was obtained after 150 minutes of MV with 0.5 mL of cold phosphate-buffered saline (PBS) with 2 mM of EDTA by inserting a standard disposable intravenous catheter (BD Insyte Autoguard ...
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bioRxiv - Immunology 2024Quote: ... the cells were cultured for 2-3 days and stained with corresponding antibodies to sort knocked-out cells using a FACS Aria flow cytometer (BD).
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bioRxiv - Immunology 2024Quote: Single cell suspensions were surface stained for 30 min on ice in flow cytometry buffer (PBS, 2% FBS, 1mM EDTA, 0.05% sodium azide) supplemented with 10% brilliant stain buffer (BD bioscience) if more than two brilliant violet dyes were used and with anti-CD16/32 (Trustain FcX ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 ng/mL ciliary neurotrophic factor (all from R&D Systems, #212-GD, #248-BD, and #257-CF, respectively) supplemented with 1.5 μM retinoic acid ...
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bioRxiv - Developmental Biology 2024Quote: ... Electroporated MEFs were sorted for GFP+BFP+ cells by fluorescence-activated cell sorting (FACS) 2 days later (BD FACS Aria3u), expanded ...
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bioRxiv - Immunology 2024Quote: ... To generate fluorescently tagged Env probes 2 μg of biotinylated Env protein was incubated with either 0.5 μg streptavidin-conjugated PE (BD Biosciences) or 0.5 μg streptavidin-conjugated BV786 (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... were incubated on ice for 30 min in the dark in 50 ul of PBS containing 2 ul of APC-labeled mouse anti-human CD4 or anti-human CD184 (CXCR4) antibody (BD biosciences 561840 and 560936 ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclei were then stained with 4’,6-diamidino-2-phenylindole (DAPI) and flowed through a fluorescence activated cell sorter (FACS) (BD FACSCanto ...
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bioRxiv - Immunology 2024Quote: ... We resuspended cells in FACSWash (1x PBS, 2% FBS, 0.2% sodium azide) and acquired events on a LSRFortessa (BD Biosciences), which we analyzed using FlowJo v10 (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... cells were suspended in PBS containing 0.5% FBS and 2 mM EDTA and were sorted with a 100-μm nozzle using BD FACSAria III (BD Biosciences). Sorted cells were pelleted and lysed in RNA lysis buffer (Zymo Research).
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bioRxiv - Cell Biology 2024Quote: ... The samples were then washed twice with DPBS containing 2% BSA and 0.01% saponin before flow cytometry analysis using a BD FACSymphony A5 Cell Analyzer (BD Biosciences) at a fixed speed ...
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bioRxiv - Immunology 2021Quote: ... and stained with anti-pSTAT1-PE (1:5 dilution, clone 4a, BD cat#612564). All flow cytometry was performed on a Beckman Coulter Cytoflex instrument.
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bioRxiv - Cell Biology 2022Quote: ... and erythrocytes were lysed using 5 ml of 1× Pharm Lyse solution (BD Pharmingen) for 3 min ...
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bioRxiv - Developmental Biology 2023Quote: ... in 1:100 for 5 min at 4 °C and were sorted by BD Influx flow cytometry in CPOS at HKUMed ...
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bioRxiv - Cancer Biology 2023Quote: ... combined with FITC Rabbit Anti-Active Caspase-3 conjugated antibody (1:5; BD Biosciences). As a positive control ...
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bioRxiv - Cancer Biology 2021Quote: ... γδ T cells were washed twice in cold FACS buffer (PBS, 5 mM EDTA, 1% bovine serum antigen) and stained with 1:20 anti-CD3-PerCP-Cy5.5 (BD Biosciences), 1:20 anti-TCRγδ-PE (BD Bioscience) ...
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bioRxiv - Immunology 2020Quote: ... CLTX-CAR T cells were incubated with GBM cells (E:T=1:1) for 5 h in the presence of CD107a antibody and GolgistopTM protein transport inhibitor (BD Biosciences). To test for CAR T cell killing potency ...
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bioRxiv - Immunology 2022Quote: ... T cells and tumor cells were co-cultured at a 1:1 effector to target (E:T) ratio for 5 hours in the presence of GolgiStop Protein Transport Inhibitor (BD Biosciences). Surface phenotype of cells was determined by flow cytometry using fluorochrome-conjugated antibodies specific for CD3 (Miltenyi Biotec ...
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bioRxiv - Microbiology 2024Quote: ... The overnight cultures of Pseudomonas aeruginosa ATCC27853 and EXT111762 were adjusted to McFarland Standard 1 and further diluted to 5 × 105 cells mL−1 in cation adjusted Mueller Hinton II broth (BD). A volume of 5 mL was distributed to each cultivation flask and supplemented with either 0.5x ...
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bioRxiv - Cancer Biology 2024Quote: ... UK), mouse monoclonal PARP (1:1000, #556494, clone 4C10-5) and mouse monoclonal cytochrome-c (1:1000, #556433) from BD Pharmingen (Heidelberg ...
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bioRxiv - Immunology 2024Quote: ... IL-5: anti-mouse IL-5 (TRFK5, BD Biosciences), biotinylated anti-mouse IL-5 (TRFK4 ...
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bioRxiv - Biophysics 2020Quote: ... After incubation cell free supernatant was collected and subjected to sandwich ELISA using IL-2 specific antibody (BD biosciences, CA, USA) as per manufacturer instruction.
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bioRxiv - Cancer Biology 2021Quote: ... The spheroids were then embedded in a collagen l/Matrigel gel mix at a concentration of approximately 4 mg/ml collagen I and 2 mg/ml Matrigel (BD Bioscience) in 24-well glass-bottomed cell culture plates (MatTek ...
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bioRxiv - Cancer Biology 2021Quote: ... single cell suspensions (1.5 x 104 cells per ml) were plated in the appropriate medium supplemented with 2% Growth Factor Reduced Matrigel (GFRM; BD Biosciences). 100μl of this mix were added per well in a 96 well ImageLock plate (Essen Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were co-stained with 4′,6-diamidino-2-phenylindole (DAPI) to measure DNA content and analyzed with a BD LSRFortessa flow cytometer (BD Biosciences) and FlowJo software (TreeStar).
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bioRxiv - Microbiology 2020Quote: ... cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Pacific Blue; BD Biosciences), anti-CD4 (L200 ...
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bioRxiv - Bioengineering 2021Quote: ... and cells collected from the wells were washed and stained with propidium iodide (2 µg/ml) to distinguish live from dead and run on a flow cytometer (BD FACSCelesta).
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bioRxiv - Cell Biology 2020Quote: ... Clonal HEK293 cells stably expressing pCRE-DD-zsGreen1 and dCas9-BFP-KRAB were generated by lentiviral transduction and fluorescence activated cell sorting for GFP and BFP on a BD FACS Aria 2 (BD Biosciences). Individual clones were expanded and tested for 1 ...
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bioRxiv - Immunology 2021Quote: ... For intracellular staining cells were fixed and permeabilized with 2% paraformaldehyde and 70% methanol or with BD Cytofix/ Cytoperm buffer (BD Biosciences) and permeabilization buffer (eBioscience ...
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bioRxiv - Developmental Biology 2020Quote: ... 2 partly overlapping holes were punched out by using a 16-gauge Jamshidi bone marrow biopsy needle (BD Biosciences., NJ, USA). The edges of the holes were trimmed with a surgical scalpel and the bottom of the defect was curetted to expose the bleeding subchondral bone ...
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bioRxiv - Molecular Biology 2020Quote: The SARS-CoV-2 S gene (Genbank accession no. NC_045512.2) was synthesized and cloned into a baculovirus shuttle vector pAcgp67B (BD Biosciences, CA, USA) using Gibson assembly ...
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bioRxiv - Immunology 2022Quote: ... The stained cells were washed and resuspended in PBS containing 2% FBS before being strained through a 70-μm cell mesh filter (BD Biosciences). SARS-CoV-2 spike-specific single B cells were gated as CD19+CD27+CD3-CD14-CD56-IgM-IgD-IgG+Spike+ and sorted into 96-well PCR plates containing 10 μL of RNAase-inhibiting RT-PCR catch buffer (1M Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... Samples were washed three times in ice cold PBS supplemented with 2% FBS and sorted on the FACSAria Fusion (BD Biosciences) with Ghost Dye Red 710 (Tonbo Biosciences ...
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bioRxiv - Immunology 2021Quote: Cultured T cells were stimulated with the cognate SARS-CoV-2 OPPs (ORF1ab-C, ORF3a, N, S, nsp12) and incubated for 4 hours in the presence of Golgiplug (BD Biosciences). Following stimulation ...
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bioRxiv - Immunology 2021Quote: ... and stained for 20 minutes at 4°C with 50 μl of antibody cocktail containing subset-specific antibodies (Supplementary Table 2) diluted in Brilliant Stain Buffer (BD Biosciences). Cells were washed in FACS buffer and fixed for 20 minutes at 4°C in 100 μl of Fixation Buffer (BioLegend).
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bioRxiv - Cancer Biology 2020Quote: ... Single cancer cells were washed with ice-cold PBS with 2% FBS and stained with Near-IR Live/Dead (BD Biosciences) on ice for 30 minutes ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were grown to logarithmic phase in SC 2% glucose medium consisting of 0.17% yeast nitrogen base (BD Difco™, 233520), 5% (NH4)2SO4 supplemented with 30 mg/L of all amino acids (except 80 mg/L histidine ...
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bioRxiv - Genomics 2021Quote: ... hPBMCs were resuspended in 1x PBS + 2% FBS and stained with a FITC-conjugated Mouse Anti-Human CD45 Antibody (Clone HI30, BD Biosciences) for 25 min on ice ...
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bioRxiv - Immunology 2021Quote: ... B cells were isolated by negative selection and resuspended in FACS buffer (PBS with 2% Fetal calf serum) followed by staining with anti-mouse CD16/CD32 (mouse Fc block; BD Biosciences) and a decoy tetramer (BV510 ...
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bioRxiv - Microbiology 2021Quote: ... The stained cells were washed and resuspended in PBS containing 2% FBS before being strained through a 70-μm cell mesh filter (BD Biosciences). RBD-specific single B cells were gated as CD19+CD27+CD3−CD14− CD56−IgM−IgD−IgG+RBD+ and sorted into 96-well PCR plates containing 10 μL of RNAase-inhibiting RT-PCR catch buffer (1M Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: Candida strains (Supplementary Table S1) were routinely grown at 30°C in yeast peptone dextrose (YPD) media (2% peptone (BD Bacto), 2% D-glucose (Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: Cells were fixed in 2% paraformaldehyde and GFP fluorescence was measured using the BD Accuri™ C6 flow cytometer (BD Biosciences). Flow sorting was performed on unfixed cells the FACS Aria III (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were blocked for 10 min in ice-cold PBS+2% heat-inactivated FBS (HI-FBS) containing anti-CD16/CD32 (BD Biosciences) and rat-γ-globulin (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... APC-R700 rat anti-mouse IL-2 (Cat No.-565186) and PE-CF594-rat anti-mouse IFN-γ were purchased from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Samples were washed three times in ice cold PBS supplemented with 2% FBS and sorted on the FACS Aria Fusion (BD Biosciences) with Ghost Dye Red 710 (Tonbo Biosciences ...
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bioRxiv - Synthetic Biology 2022Quote: ... samples of 1×106 per mL pooled CAR T cells with 2×106 per mL Nalm 6 were centrifuged at 300 × g for 2 min in 96-well flat-bottom plates and incubated in HTCM with 1x Brefeldin-A/GolgiPlug (BDBiosciences #555029), 1x Monensin/GolgiStop (BD Biosciences #554724) ...
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bioRxiv - Molecular Biology 2022Quote: ... CD4+ T cells or MT-2 cells were activated with media containing 50 ng/mL anti-CD3 (eBioscience) and 100 ng/mL anti-CD28 (BD Biosciences) antibodies.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were resuspended in 0.5 mL PBS to achieve a final density of 2×105 cells prior to acquisition and were then analyzed by a FACSCalibur flow cytometer (BD Biosciences). The surface marker expression of human MSCs thus enriched was assessed by using flow cytometry following 2 passages ...