Labshake search
Citations for Becton, Dickinson and Company :
1151 - 1200 of 7577 citations for 2 Bromo 1 5 bromofuran 2 yl ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... cells were fixed for a minimum of 20 minutes with 2% paraformaldehyde and acquired immediately using a FACS Symphony A3 (BD Biosciences). Where intracellular staining was performed ...
-
bioRxiv - Immunology 2022Quote: FACS-sorted Tregs were plated at 50,000 cells/well in round bottom 96 well plates pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) and soluble anti-human CD28 (1 μg/ml ...
-
bioRxiv - Pathology 2023Quote: ... Placentas were perfused for 3 hours and 1 ml (T4 transfer experiments) or 2 ml (TRIAC transfer experiments) samples were collected from the reservoirs into blood collection tubes (BD vacutainer) at the time points indicated in the figures ...
-
bioRxiv - Immunology 2022Quote: ... The cells were fixed in 2% paraformaldehyde and GFP expression measured and quantitated using a FACS Canto II flow cytometer and FlowJo software (BD Biosciences). IC50 values were calculated using Prism software (GraphPad/Dotmatics).
-
bioRxiv - Developmental Biology 2023Quote: ... GFP and mCherry+ cells were collected in 50 µl 2% BSA in PBS via fluorescence-activated cell sorting (BD LSR Fortessa) and processed for single-cell RNA sequencing analysis based on 10X Genomics (10X Chromium system ...
-
bioRxiv - Immunology 2023Quote: ... followed by staining with a cocktail of mAbs and four fluorochrome-conjugated spike proteins in staining buffer (2% FBS/PBS) supplemented with Brilliant Stain Buffer Plus (BD Biosciences). The stained cells were fixed (Lysing Solution ...
-
bioRxiv - Cancer Biology 2023Quote: ... were stained with the specified antibodies on ice for 30 min in D-PBS supplemented with 2% FBS in the dark and analyzed using a Fortessa or Symphony A3 (BD Biosciences). Data was analyzed using FlowJo10 (Tree Star Inc. ...
-
bioRxiv - Developmental Biology 2023Quote: ... resuspended in 300 μl of 2% FBS in PBS with 1000 ng/μl DAPI and processed by FACS on a Melody Cell Sorter (BD Biosciences) using the BD FACSChorus software ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were washed with PBS three times and resuspended in FACS buffer (PBS with 2%FBS) for analysis on BD Biosciences LSR Fortessa using a 488-nm laser and 530-nm/30 filter (BD Biosciences). Data analysis was performed with FCS Express 7 (De Novo Software) ...
-
bioRxiv - Cancer Biology 2023Quote: C4-2 cells were cultured and lifted from plates by trypsinization then re-suspended in 100 mL of 50% Matrigel (BD Biosciences) and saline mixture followed by subcutaneous injection in two separate groups of castrated and non-castrated athymic nude mice (age at least 6 weeks old) ...
-
bioRxiv - Biophysics 2022Quote: ... The FITC ERF values were assigned to both 2 μm and 6 μm beads using a specific lot of FITC-FC Bead (BD Biosciences) as a calibrator with known ERF value ...
-
bioRxiv - Microbiology 2023Quote: ... PBMCs were then stained with live/dead and cell surface markers in MACS solution (Miltenyi) supplemented with 2% BSA (Miltenyi) prior to permeabilization with Cytofix/Cytoperm (BD Biosciences) and staining with intracellular markers in Perm/Wash (BD Biosciences) ...
-
bioRxiv - Microbiology 2023Quote: ... PBMCs were then fixed in 2% formaldehyde and stored at 4°C until flow cytometry on an LSR II flow cytometer (BD Biosciences). Cell surface markers included CD3 (clone 17A2 ...
-
bioRxiv - Immunology 2023Quote: ... T cells isolated from lymph nodes or tumors were mixed with 3×106 congenically marked B6 splenocytes and incubated with 1 μg/mL of OVA peptide and/or 2 μg/mL of GP peptide for 4-5h at 37°C in the presence of GolgiPlug (BD Biosciences). After staining for cell surface molecules ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were then selected in puromycin (1μg/ml) 2 days after transduction and sorted for their EGFP fluorescence after 6 additional days using FACS ARIA III (BD Biosciences). Cells were grown for recovery and collected for genomic DNA extraction with DNeasy Blood and Tissue kit (Qiagen).
-
bioRxiv - Immunology 2023Quote: ... Antibody cocktails for surface proteins were prepared in PBS + 2% FBS and Brilliant Stain Buffer (50 µl/100 µl of cocktail, BD Biosciences) and added to the cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... Anaerobic bacteria colonies were counted after incubating these agar plates for 2 days using the GasPak anaerobic gas generating pouch system (BD Biosciences).
-
bioRxiv - Microbiology 2023Quote: ... GYM (glucose 4 g, yeast extract 4 g, malt extract 10 g, CaCO3 2 g, and Bacto Agar (BD Difco™) 20 g ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... and the second model investigated ii) four exposures from the first eating event (AK-20, BD, KB, LT & NH; Model 2). In each model ...
-
bioRxiv - Immunology 2023Quote: ... The tube was then stored in the dark at 4°C for up to 2 hours prior to acquisition on a LSRII or FACSLyric analyzer (BD Biosciences).
-
bioRxiv - Immunology 2023Quote: ... solution for 20 minutes followed by 20 minutes of antibody cross linking on ice with 2 µg/mL goat-anti mouse solution (BD, #53998). T cells were then activated via an incubation period at 37 °C in FACS buffer (PBS with 2% FBS and 2 mM EDTA ...
-
bioRxiv - Cancer Biology 2023Quote: ... or FAK-KD cell lines were generated by injecting a total of 2×106 MDA-MB-231/Dendra2 cells resuspended in 20% collagen I (BD Biosciences) in PBS into the lower left mammary gland of SCID mice ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell pellets were dissolved in 2% FBS in PBS and flow cytometry was performed using an Accuri C6 Plus (BD Biosciences) or Citole Flow Cytometer (Beckman Coulter).
-
bioRxiv - Genomics 2023Quote: ... at least 2 × 106 cells were fixed and permeabilized using the Fixation/Permeabilized Solution Kit with BD GolgiStop TM (BD Biosciences) following the manufacturer’s recommendations ...
-
bioRxiv - Immunology 2023Quote: ... The proteases were inactivated by adding 10% fetal bovine serum and 2 mM EDTA and the cell disaggregated by passing them through a 40 μm nylon sieve (BD Bioscience). Single cells were then washed with 1% Bovine serum albumin (BSA ...
-
bioRxiv - Immunology 2023Quote: ... Samples for flow cytometry were fixed in 2% paraformaldehyde solution in PBS and analyzed using a LSRII flow cytometer (BD Biosciences) and FlowJo software (Tree Star ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were suspended in 350 µL of FACS buffer (1XDPBS, 2% FBS, 25mM HEPES, 2mM EDTA) and was analysed by flow cytometry BD LSRFortessa (BD Biosciences). 10,000 cells were analysed for each condition ...
-
bioRxiv - Bioengineering 2024Quote: ... for viability assessment and fixed with 2% paraformaldehyde (30 min incubation) until analysis by flow cytometry using an LSR Fortessa (BD Biosciences). For assessment of NP-associated fluorescence in a fluorescence plate reader ...
-
bioRxiv - Immunology 2024Quote: ... Cell culture supernatants were assayed for IL-2 using capture and biotin-labeled detection antibody pairs (BD Biosciences, Franklin Lakes, NJ). Detection was performed with HRP-conjugated streptavidin (BD Biosciences ...
-
bioRxiv - Immunology 2024Quote: ... the cells were fixed with 2% (w/v) paraformaldehyde and stored at 4°C before analysis with FACS Celesta Cell Analyzer (BD Biosciences). Data were analyzed using FlowJo software version X.0.7 (Tree Star ...
-
bioRxiv - Cell Biology 2024Quote: ... was added to a final concentration of 2 μg/mL and cells examined by flow cytometry using a FACSLyric flow cytometer (BD Biosciences). For controls (negative ...
-
bioRxiv - Immunology 2024Quote: T cells were restimulated with 3 μg/ml plate-bound anti-CD3 and 2 μg/ml soluble anti-CD28 or PMA/Ionomycin in the presence of GolgiPlug protein transport inhibitor (BD Biosciences) for 6 hours ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Isolates from the CNRMA in France were subcultured on 2% malt extract agar (Oxoid) and potato dextrose agar (BD Diagnostic Systems) for 5 days at 30 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were resuspended in DPBS containing 2% FCS and 1mM EDTA and immediately analyzed on a FACSCanto II or FACSymphony A1 Cell Analyzer (BD Biosciences). For yH2AX measurements in U2OS cells ...
-
bioRxiv - Genomics 2024Quote: ... After 1-2 weeks, mCherry+ cells were sorted by FACS (Flow Cytometry Core Facility, EPFL) using FACSAriaII or FACSAriaFusion flow cytometers (BD Biosciences) and dispensed as single cells into 384-well plates containing conditioned medium (50:50 fresh 10% FBS ...
-
bioRxiv - Microbiology 2024Quote: Splenocytes or lung cells were cultured in presence or absence of 2 μL/mL of cell stimulation cocktail (eBioscience, 00-4975-93) and brefeldin A (BD, 555029) in RPMI complete media for 6 hours at 37°C in a humidified chamber and 5% CO2 ...
-
bioRxiv - Developmental Biology 2024Quote: ... hPSCs were seeded as single cells (2-4×105 cells/ml, see hPSC maintenance) on coated 12-well plates (BD Falcon). Cells were constantly maintained at 37°C in 5% CO2 with 95% air.
-
bioRxiv - Microbiology 2024Quote: ... The plates were incubated for 2 h at 37 °C before the bacteria were resuspended in a final volume of 100 µl MH broth (BD Biosciences). Serial dilutions in MH broth were carried out and 5 µl of each dilution was spotted in technical duplicates on MH agar plates supplemented with 50 µg/ml ampicillin for calculation of total number of colony forming units (CFUs ...
-
bioRxiv - Cell Biology 2024Quote: ... The supernatant was discarded and the cells were resuspended in 2 mL red blood cell lysis buffer (1X, BD Biosciences, 555,899), mixed by pipetting up-and- down ...
-
bioRxiv - Immunology 2024Quote: ... Purified B cells were seeded at a density of 2-10 * 103 cells per well in 96-well Microtest U-bottom plates (BD Falcon) and were cultured with αCD40 (150 ng/mL ...
-
bioRxiv - Immunology 2024Quote: ... Samples for flow cytometry were fixed in 2% paraformaldehyde solution in PBS and analyzed using a LSRII or A5 flow cytometer (BD Biosciences) and FlowJo software (Tree Star ...
-
bioRxiv - Biophysics 2024Quote: ... After incubation cell free supernatant was collected and subjected to sandwich ELISA using IL-2 ELISA kit (BD biosciences, CA, USA) as per manufacturer instruction ...
-
bioRxiv - Cancer Biology 2024Quote: ... Final cell pellets were resuspended at a cell density of 2×106 cells/ml in 3% FBS in PBS and analyzed at the UTSW Flow Cytometry Core Facility (FACSCalibur, BD Biosciences). FlowJo software was used to analyze mean fluorescent intensity (RRID:SCR_008520).
-
bioRxiv - Cancer Biology 2022Quote: ... and blocked in 5% milk in TBST before staining with Fibronectin (BD #610078, 1:10,000), ZEB1 (LSBio ## 1:2000) ...
-
bioRxiv - Neuroscience 2024Quote: ... 5 Matrigel drops were transferred to 1 ml cold Cell Recovery Solution (BD Biosciences, 354253) in a 15 ml Falcon ...
-
bioRxiv - Bioengineering 2024Quote: ... iii) two-cell PD-1 campaign-Fc-block (5 μg/ml; BD Biosciences cat# 553142), anti-mouse Ly6A/E-PE/Cy7 (5 μg/ml ...
-
bioRxiv - Neuroscience 2020Quote: ... Blood samples were diluted 1:5 with PBS and 10 µl aliquots were added to 1 ml of BD Retic-Count reagent (BD Biosciences). After a 60 min incubation in the dark at RT ...
-
bioRxiv - Microbiology 2021Quote: ... 0.5–1 million cells were stained with one of the following primary antibodies: mouse anti-PSGL1 antibody (KPL-1) (BD Pharmingen), mouse anti-CD43 antibody (1G10 ...
-
bioRxiv - Microbiology 2023Quote: ... The seawater samples were then diluted with culture media to a concentration of 5 cells mL-1 and dispensed (1 mL per well) into 48-well microplates (BD Falcon). The plates were incubated at 20 °C for 1 month in dark or under LED light (Philips ...
-
bioRxiv - Cancer Biology 2021Quote: ... The bottom layer of soft agar was prepared by mixing 2 × growth medium and 1.5% noble agar (BD Biosciences, San Jose, CA) at a 1:1 ratio and the mixture was poured into 6-well plates ...