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Citations for Becton, Dickinson and Company :
9551 - 9600 of 9716 citations for Anti PIF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Resuspended with 75 μL FACS buffer and stained with 0.5 μL alexa Fluor 488-conjugated anti-alkaline phosphatase (BD Pharmingen, 561495) and 25 μL of PerCP-Cy5.5-conjugated anti-CD117 (BD Pharmingen 333950 ...
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bioRxiv - Cell Biology 2022Quote: Freshly dissected LW whole mount preparations from 6 wild-type and 4 Lrp2-/267 mice were incubated with rat anti-CD24 conjugated with Phycoerythrin (PE; 1:100; BD Pharmingen) in Neurobasal medium (Gibco ...
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bioRxiv - Immunology 2022Quote: ... Acquisitions were done on a FACS Canto II flow cytometer (Becton Dickinson) with the following mAbs: Fluorescein (FITC)-coupled 145-2C11 (anti-CD3, BD Biosciences), FITC-coupled HL3 (anti-CD11c ...
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bioRxiv - Molecular Biology 2022Quote: Flow cytometry analysis was performed using the following cell surface markers: anti-CD11b conjugated to phycoerythrin (PE, BD Biosciences, clone ICRF44) and anti-CD44 allophycocyanin (APC ...
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bioRxiv - Physiology 2022Quote: ... Sections were washed 3 times in wash buffer then stained for Alexa Fluor 555 mouse anti-E-cadherin (BD, Cat. 560064) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Thermo Fischer) for one hour at room temperature and co-stained with anti-γH2AX coupled to Alexa 647 (560447, BD Biosciences) and anit-mCherry coupled to Alexa 594 (M11240 ...
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bioRxiv - Molecular Biology 2022Quote: ... blocked with Sea-block buffer (37527- Thermo Fischer) for one hour at room temperature and co-stained with Alexa 647-coupled mouse anti-γH2AX (560447, BD Biosciences) and FITC coupled goat anti-GFP (NB100-1771 ...
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bioRxiv - Immunology 2022Quote: ... Human CD34+ stem cell purity was monitored by anti-human CD34 FITC (clone:581) and 7-AAD viability dye using LSR Fortessa X-20 flow cytometer (BD Biosciences).
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bioRxiv - Biophysics 2024Quote: ... cells were washed twice in PBS + 2% FBS and stained with mouse anti-human CD45 Pe-Cy5 (BD Bioscience, clone HI30) and mouse anti-human CD107a Alexa Fluor 647 for 30 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2024Quote: ... the SRBCs were washed in a standard FACS buffer (1000g for 10 minutes) and stained with anti-mouse IgG-BV605 (BD, 405327) or anti-IgM (BD ...
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bioRxiv - Pathology 2024Quote: Peripheral venous blood from patients with CLI and controls was collected into ethylenediaminetetraacetic acid (EDTA) anti-coagulated tubes (BD Vacutainer, UK). 17.5 ml of whole blood was added to sterile 50 ml falcon tube and diluted 1:1 with cold sterile DMEM ...
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bioRxiv - Neuroscience 2024Quote: ... PBMCs were then exposed to serum and CSF samples and stained exactly as done for CNS cells except for the addition of an anti-CD3 APC-H7 Ab (BD Biosciences) during the Aqua Live/Dead staining step ...
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bioRxiv - Neuroscience 2024Quote: ... OPs were obtained by immuno-panning from single-cell suspensions of dissociated P6-7 mouse cortices using anti-PDGFRA immunoglobulin (558774, BD Pharmingen) as previously described (Emery and Dugas ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies (0.5 μg/mL) and dyes (concentrations provided below) were added: Anti-CD11b-FITC (clone M1/70, catalog no. 553310; BD Biosciences), anti-Ly6G-APC (clone 1A8 ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies (0.5 μg/mL) and dyes (concentrations provided below) were added: Anti-CD11b-FITC (clone M1/70, catalog no. 553310; BD Biosciences), anti-Ly6G-APC (clone 1A8 ...
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bioRxiv - Immunology 2024Quote: ... wells were incubated with 100 µL of biotinylated anti-IgG1 (1 µg/mL in PBS with 2% FBS, clone A85-1, BD Biosciences) for 2 hours at RT ...
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bioRxiv - Immunology 2024Quote: ... and 5×104 of each were combined in 96-well U-bottom plates with anti-CD40 (100 ng/mL, clone HM40-3, BD Biosciences) in the presence or absence of OVA (10 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies (0.5 μg/mL) and dyes (concentrations provided below) were added: Anti-MPO-FITC (clone M1/70, catalog no. 553310; BD Biosciences), Fc Block ...
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bioRxiv - Cell Biology 2024Quote: ... and screened for protein levels via Western blots with monoclonal mouse anti-IQGAP1 (1:1000; 610612, BD Biosciences, East Rutherford, NJ), polyclonal rabbit α-tubulin (1:2500 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed 2x with 1% FBS in PBS and incubated in 1% FBS in PBS with APC-conjugated mouse anti-human CD68 (BD Biosciences) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... all samples were filtered through a 70-μm nylon mesh and blocked with anti-Fc CD16/32 (1:40; BD Biosciences). For FOXP3 staining ...
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bioRxiv - Immunology 2022Quote: FACS-sorted Tregs were plated at 50,000 cells/well in round bottom 96 well plates pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) and soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... The cell pellet was resuspended in 200 μl ice-cold DMEM and incubated sequentially on ice with purified rat anti-mouse FDC-M1 (BD Biosciences) or rat IgG2c ...
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bioRxiv - Cancer Biology 2023Quote: ... digested lung cells were resuspended in 400 μl of buffer with 5 μl of anti-CD31-FITC (BD Biosciences, CA, USA), 5 μl of anti-CD45-APC (BD Biosciences) ...
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bioRxiv - Bioengineering 2023Quote: ... Gates were determined using the isotype controls mouse anti-IgG2b κ (APC-conjugated, Clone 27-35, 1:5, BD Biosciences, US) and mouse anti-IgG2a κ (PE-Cy7-conjugated ...
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bioRxiv - Biophysics 2022Quote: ... samples were first resuspended in 15.5 μL PBS/0.1% adBSA and incubated with 0.5 μg of rat anti-mouse CD9-PE (Clone: KMC8, IgG2a, κ lot 7268877, BD Biosciences) or matched isotype antibodies (Rat IgG2a ...
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bioRxiv - Immunology 2023Quote: ... IL- 10 secretion was determined by ELISA in supernatants harvested after a 24 hour restimulation with anti-CD3 (555336, BD Biosciences) and anti-CD28 (555725 ...
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bioRxiv - Genetics 2023Quote: ... and CD43-B cells were cultured at a density of 5×105 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). Cultured cells were maintained daily at a density of 0.5-1×106 cells per ml ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated for 15 min at 4 °C with a purified mouse anti-rat CD32 FcγII (clone D34-485, Rat BD Fc Block) to block unspecific binding ...
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bioRxiv - Immunology 2023Quote: 50 × 103 effector cells were cultured alone or together with 5 × 103 tumour cells for 4 h at a 10:1 ratio in the presence of anti-CD107a-PE mAb (clone: H4A3; BD Biosciences) and monensin (3 μM ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained on ice for 30min in FACS buffer with 1:100 dilutions of biotin-conjugated-HA and purified rat anti-mouse CD16/CD32 (Mouse BD Fc Block™, Clone 2.4G2, BD Biosciences), then stained on ice for 30min in FACS buffer with 1:1000 dilution of allophycocyanin (APC)-conjugated streptavidin ...
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bioRxiv - Immunology 2022Quote: ... GFP expression in peripheral blood B and T cells was screened using anti-CD19-PE (BD, clone 1D3, dilution 1/100), anti-CD3e-PerCP-Cy5.5 (eBioscience ...
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bioRxiv - Cell Biology 2022Quote: ... for epitope retrieval and incubation with primary anti-Collagen Type II (DSHB II-II6B3) followed by a biotinylated secondary and Streptavidin-HRP (BD Biosciences). II-II6B3 was deposited to the DSHB by Linsenmayer ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with 2% ECL prime blocking reagent in PBS-T (Amersham; #RPN418) and incubated against AP-2 (mouse-anti-AP50, BD, #A611351) and GAPDH (rabbit-anti-GAPDH ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were incubated with Fc block (anti-mouse CD16/CD32, 1 μg for 1x106 cells, BD Biosciences, Cat# 553142) at 4°C for 30 min ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated at 4°C for 30 min with APC-conjugated rat anti-mouse CD31 (1:200, BD Pharmigen, 551262). DAPI (5 mg/ml ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL, BD Biosciences, Franklin Lakes, NJ, USA). A 1:1,000 dilution of anti-mouse IgG Alexa-647 (stock 1 mg/mL ...
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bioRxiv - Immunology 2023Quote: ... Single cells were then washed with 1% Bovine serum albumin (BSA) in PBS and blocked with anti-Fcγ receptor (BD Biosciences). Human peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Cell Biology 2023Quote: ... E8.5 embryos were fixed with 4% paraformaldehyde (PFA) in PBS at 4°C for 3 h and incubated with FITC-conjugated anti-EEA1 (1:100, BD Bioscience) or Cy3-labeled anti-mouse IgG (1:500 ...
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bioRxiv - Immunology 2023Quote: ... mock or INHBA overexpression tumor cells (CT26 and MC38) and shCtrl or shINHBA CT26 cells were analyzed for IFNGR1 (BV421 Rat Anti-Mouse CD119; 740032, BD Bioscience) and IFNGR2 expression (Hamster Anti-Mouse IFNGR2 Monoclonal antibody ...
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bioRxiv - Immunology 2023Quote: ... mock or INHBA overexpression CT26 cells were directly analyzed for PD-L1 expression (BV650 Rat Anti-Mouse CD274; 740614, BD Bioscience) by flow cytometry ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed two times with 150 µL PBS (centrifuged 5 min at 400 × g at 4 °C) and resuspended in 50 µL Fc-block (1:50, Purified Rat Anti-Mouse CD16/CD32, BD, #553142) diluted in FACS buffer (1x PBS/2% FBS/2mM ...
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bioRxiv - Cell Biology 2023Quote: ... digested lung cells were resuspended in 400 μl of buffer with 5 μl of anti-CD31-FITC (BD Biosciences, CA, USA), 5 μl of anti-CD45-APC (BD Biosciences) ...
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bioRxiv - Physiology 2024Quote: ... The 2X stain cocktail included the following antibodies (final dilutions are all 1:300 unless otherwise indicated): rat anti-mouse SiglecF-BV421 (1:400, clone E50-2440, cat# 562681 BD Horizon), rat anti-mouse/human CD11b-Pacific Blue (clone M1/70 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were activated for 3 days with 1 μg/mL/million cells of purified NA/LE mouse anti-human CD3 and CD28 (BD Biosciences) and infected with HIVBaL at 0.01 ng/106 cells ...
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bioRxiv - Immunology 2024Quote: T cells were restimulated with 3 μg/ml plate-bound anti-CD3 and 2 μg/ml soluble anti-CD28 or PMA/Ionomycin in the presence of GolgiPlug protein transport inhibitor (BD Biosciences) for 6 hours ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Fixed cells were stained for 30 min at 4° C with PerCP-Cy5.5 mouse anti-human CD195 (CCR5, BD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... fixed and permeabilized with the FoXP3 Transcription Buffer Kit (eBioscience) and intracellularly stained with PE-CF594-conjugated anti-Foxp3 (BD Biosciences) and PE-conjugated anti-IL-10 (BD Biosciences) ...
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bioRxiv - Molecular Biology 2024Quote: ... unsorted, sorted, and depleted fractions, were labelled with mouse anti-ASGPR1-PE (1:20 dilution, to detect porcine hepatocytes) (BD Pharmigen), rat anti-OC2-2F8 ...