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Citations for Becton, Dickinson and Company :
9501 - 9550 of 9716 citations for Anti PIF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Tissues were blocked with 5% donkey serum and 0.1% Triton X-100 in PBS for one hour at room temperature and incubated with 1:1000 of anti-CD47 (BD Biosciences), 1:500 of anti-TSP1 (Abcam) ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used for analysis of the H2B-GFP LR population (Figure 5): rat anti- Cd74-BUV395 (Clone In-1, BD, 740274), rat anti-Cd45-BUV563 (Clone 30-F11 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed with PBS containing 5 mM EDTA and 3% FBS (FACS Buffer) and then blocked with 1% anti-mouse CD16/CD32 (BD biosciences), 5% normal rat serum and 5% normal mouse serum in FACS buffer for 10 minutes at RT ...
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bioRxiv - Cell Biology 2021Quote: ... cells were sorted after thawing by fluorescence-activated cell sorting (FACS) for the CD34+CD38- population using 10 μL of anti-CD34 APC (560940, BD Biosciences), 10 μL of anti-CD38 FITC (555459 ...
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bioRxiv - Immunology 2021Quote: ... cells were initially fixed and permeabilized for 1 hour using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) and then stained using the PE-conjugated anti-p53 Set (BD Biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... B cells were isolated by negative selection and resuspended in FACS buffer (PBS with 2% Fetal calf serum) followed by staining with anti-mouse CD16/CD32 (mouse Fc block; BD Biosciences) and a decoy tetramer (BV510 ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular staining of the cytokines was performed after fixation and permeabilization using APC conjugated anti-human IFNγ (BD Bioscience, Cat# 551385), anti-human IL2 (BD Bioscience ...
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bioRxiv - Microbiology 2020Quote: ... during 15 min at 4 °C in MACS buffer followed by incubation with anti-CD45 1:100 (clone 30F-11, BD biosciences), anti-EpCAM 1:100 (clone G8.8 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were blocked for 10 min in ice-cold PBS+2% heat-inactivated FBS (HI-FBS) containing anti-CD16/CD32 (BD Biosciences) and rat-γ-globulin (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2021Quote: ... CD34+CD38-cells from healthy controls and MDS patients cells were also labeled with the BV605-conjugated anti-CD38 (BD Biosciences) (#562666) ...
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bioRxiv - Genomics 2020Quote: ... 3 times 5 min washes with TNT were followed by a 1 h incubation with biotinylated goat anti-rat (559286, BD biosciences) 1:100 in TNB ...
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bioRxiv - Genetics 2022Quote: ... cells were resuspended in 90 μL staining buffer and incubated with 10 μL of anti-human CD21-PE (Cat #555422, BD Bioscience), anti-human CD55-PE (MHCD5504 ...
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bioRxiv - Developmental Biology 2022Quote: ... Hbbth3/+ and p21-/-/Hbbth3/+ mice and stained for Ki-67 using the Mouse Anti-Ki-67 Set (BD Biosciences, Cat# 556026). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... TCR expression on transfected Jurkat cells was assessed by staining with phycoerythrin (PE)-conjugated mouse anti-human CD3 (BD Pharmingen 555333); TCR expression was unaltered by Csk overexpression.
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bioRxiv - Molecular Biology 2022Quote: Concentrations of TNF-α and IFN-γ in sera on day 21 after allogeneic BMT were measured with specific anti-mouse ELISA kits from BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Myoblasts were stained with 7-AAD (cell viability) and with the positive selection marker anti-CD56 AF647-conjugated (clone R19-760, BD biosciences). Of note ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked with Purified Rat Anti- Mouse CD16/CD32 Mouse BD Fc Block™ (2.4G2, BD Pharmingen, 1:1000). Cell surface antigens were stained in PBS containing 2 % (vol/ vol ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed with PBS in the presence of 2 % BSA and incubated with anti-IFN-γ-PE-CY7 (BD Biosciences), anti-IL-2-APC (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... For each sample 200,000 cells were incubated with suitable concentrations of CD4 Nbs coupled to CF568 in combination with 1:500 dilution of anti-CD3-FITC (BD Biosciences) at 4°C for 30 min ...
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bioRxiv - Immunology 2020Quote: ... followed by staining for 3 days at 37 °C with 1:100 dilutions of BV421-labeled mouse anti-human CD35 clone E11 (BD Biosciences) and Alexa Fluor 488-labeled mouse anti-Ki67 clone B56 (BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... to stain for CD69 receptor using 5 µL (100 µg/mL) of PreCP-Cy ™ 5.5 mouse anti-human CD69 (BD Biosciences). Staining was done for 45 mins on ice then washed one time with 2 mL RPMI-1640 medium and reconstituted in 250 µL RPMI-1640 medium ...
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bioRxiv - Immunology 2022Quote: ... 0.75 × 105 naïve T cells were cultured for one or two days with plate-bound anti-CD3ε (2 µg/ml, clone 145-2C11; BD Biosciences) in differentiation medium consisting of RPMI1640 supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... whereas 100 µl PBS+/+ containing 20 ng/ml recombinant IL-1β supplemented with 100 µg/ml PTX3 and/or 100 µg/ml mouse anti-human CD62P (clone AK-4, BD Bioscience) was added to the apical side (upper chamber) ...
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bioRxiv - Immunology 2022Quote: Suspensions of human Tconv cells or Treg cells were stained with V500-conjugated anti-human CD4 (clone RPA-T4, BD Biosciences), PE-Cy7-conjugated anti-human CD25 (clone BC96 ...
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bioRxiv - Developmental Biology 2019Quote: ... the cells were resuspended with blocking buffer (5% BSA in DPBS) and stained with PE-Cy7 rat anti-CD31 (1:400, 561410, BD biosciences), APC mouse anti-EpCAM (1:400 ...
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bioRxiv - Immunology 2019Quote: ... After washing in PBS and incubated for 1 hour at RT with secondary donkey-anti-rat rhodamine red 1:100 (both from BD Pharmingen) in antibody buffer ...
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bioRxiv - Cell Biology 2019Quote: ... T cell activation was measured with a PE-conjugated anti-hCD69 on CD3+ gated viable cells by flow cytometry (BD LSRFortessa, BD Biosciences). For T cell proliferation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Staining panel for CT26 and 4T1 mouse syngeneic model is as follows: anti-mouse CD4 (BD Biosciences, cat#563790, clone# GK1.5), anti-mouse CD44 (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturer’s protocol and intracellular staining was performed using anti-BCL-6-AF647 (clone K112-91, 1:100, BD, cat#561525).
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bioRxiv - Immunology 2020Quote: ... Cells with SARS-CoV-2 peptides and positive control were co-stimulated with CD28/CD49d at a final concentration of 5 μg/ml (anti-human CD28/CD49d Purified Clone L293 L25 BD Biosciences). Basal IFN-γ production by PBMCs was included as background control in the absence of stimulation and co-stimulation ...
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bioRxiv - Immunology 2020Quote: ... then washed with 2% FCS - PBS and stained with Mouse anti-IRF-7-PE (pS477/pS479, clone K47-671; BD Biosciences) or Mouse anti-NF-κB p65-Alexa Fluor 647 (pS529 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then blocked for 5 minutes at room temperature using anti-mouse CD16/32 Fc block (1/50) (BD Biosciences). Cells were then incubated with the staining (Ly6g-APCCy7 (1A8) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with 1 × PBS (30 s) and stained on ice in the dark for 30 min with anti-CD3 (BD, 557705), anti-CD4 (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... The bound antibodies were detected with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:4000) for 1 hour at 37 °C and developed with tetramethyubenzidine (TMB) substrate (BD PharMigen), followed by measuring the absorbance of individual wells at 450 nm using an ELISA reader (Bio-rad mod.550) ...
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bioRxiv - Immunology 2021Quote: ... cells were stained intracellularly using a rabbit non-conjugated monoclonal anti-phospho-SNAP23(Ser95)(Karim et al., 2013) for 40 minutes in Perm/Wash Buffer 1x (BD Bioscience) with 0.5% of rabbit serum ...
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bioRxiv - Pathology 2021Quote: Cells were fixed in 2% PFA for 15 minutes at room temperature and incubated with mouse anti-human VE-Cadherin (BD Sciences) at 4°C overnight as previously described 36 ...
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bioRxiv - Immunology 2020Quote: ... lung T cells were incubated at 37°C for 5 hours with 5 μM Bl-Eng2 or calnexin peptide and 1μg anti-mouse CD28 (BD, cat 553294). After 1h ...
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bioRxiv - Immunology 2021Quote: ... The expression of the standard activation markers CD69 and CD134 (OX40) was quantified on the cell surface using anti-CD69–FITC (clone L78; BD Biosciences) and anti-CD134–BV711 (clone ACT35 ...
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bioRxiv - Immunology 2021Quote: ... Intracellular stains were performed using anti-T-beteFluor66O (clone 4B10; eBioscience) in conjunction with a Transcription Factor Buffer Set (BD Biosciences). Samples were acquired using an LSR Fortessa or a FACSCanto II (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... anti-CD3 and NKp46+CD3- cells were sorted at the Case Western Reserve University Flow cytometry core using (BD Biosciences Aria) (purity>99%) ...
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bioRxiv - Immunology 2021Quote: Cells were co-cultured in R10 medium alone or in presence of K562 cells for 6 hours in the presence of anti-human CD107a (H4A3, FITC, BD Biosciences). Golgi plug and Golgi stop (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... for 10 minutes at room temperature before stimulation with or without cognate antigen at 10 μg/mL in the presence of 10 μL/mL anti-CD28/49d (BD Biosciences) and 1x protein transport inhibitor (eBioscience ...
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bioRxiv - Immunology 2021Quote: ... the sera were diluted 1/10 with PBS containing 1% (w/v) BSA and loaded onto anti-mouse IgE coated (BD biosciences) plates anti-OVA-HRP antibodies (AbD Serotec ...
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bioRxiv - Immunology 2021Quote: ... Resting- or in vitro-generated effector CD4+ or CD8+ T cells (105 cells/well) were stimulated with 1 μg/ml plate-bound anti-mouse CD3 mAb (BD Bioscience) in combination with different concentrations of P3CSK4 ...
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bioRxiv - Immunology 2020Quote: ... Mouse neutrophils were generally purified from the BM by positive magnetic selection with anti–Ly-6G PE-conjugated Ab (1A8; BD Biosciences) and anti-PE microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... cells were washed with 1x permeabilization buffer and stained with intracellular cytokines (IFN-L (BV421 Rat Anti-Mouse IFN-γ, Clone: XMG1.2, cat # 560660, BD Biosciences) for 30 mins at 4°C ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse IFN-γ ELISA set (Cat no. – 555138) and anti-human IL-10 ELISA set (Cat no. – 555157) were purchased from BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: Cells either from culture or from transwell assay were washed in PBS and incubated in 2% FBS in PBS with anti-CCR7-FITC (1:25, #561271, BD Biosciences) and anti-ROR1-APC (1:25 ...
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bioRxiv - Immunology 2022Quote: Single cells suspensions were incubated a mixture containing 50 µg/ml rat anti-mouse CD16/CD32 (BD Biosciences, Franklin Lakes, USA), normal Syrian hamster serum (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... the cell suspension was then incubated on two PDGFRα plates coated with rat anti-PDGFRα IgG (BD Biosciences, 558774; 1:500). The adherent cells were washed with PBS to remove all antigen-negative nonadherent cells ...