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Citations for Becton, Dickinson and Company :
9251 - 9300 of 9716 citations for Anti PIF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and intracellular staining was performed following fixation and permeabilization with anti-Tbet PE-CF594 (O4-46; BD Biosciences), anti-Gata3 AF700 (TWAJ ...
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bioRxiv - Immunology 2024Quote: ... 2mM EDTA) and incubated with Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 (BD Biosciences) to block non-specific staining ...
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bioRxiv - Microbiology 2024Quote: ... The purity of isolated CD34+ cells were accessed by flow cytometry using mouse anti human CD34 (BD Bioscience).
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bioRxiv - Immunology 2024Quote: ... and positive control cells were incubated in plates coated with anti-CD3 (1μg/ml) (clone HIT3a, BD Biosciences) or PHA (2 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... Mononuclear cells were washed three times with PBS and CD34+ cells were isolated as described 35 using rhesus macaque-specific antibodies to CD34 (clone 563; BD Pharmingen, San Jose, CA, USA) and the anti-PE MACS magnetic bead system (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2020Quote: ... The fluorescent signal from GLI1 OE and control cell samples stained for cTnT-positivity was normalized to fluorescent signal from cells stained with a BV421-conjugated isotype control IgG antibody (BD Biosciences 562438, Lot 8242926, X40). Fluorescence was quantified ...
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bioRxiv - Immunology 2020Quote: ... Single cells were stained with the different fluorescent antibodies (as indicated in the figure legends) and DsRed on the BD LSR II flow cytometer (BD Biosciences, San Jose, CA, USA). Analyses were completed using FlowJo analysis software (FlowJo ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™, BD Biosciences, San Diego, CA, USA), 5 μg/ml brefeldin A (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were probed with different combinations of conjugated primary antibodies (Table S1) and analyzed by flow cytometry (Canto II AIG, BD Biosciences, Franklin Lakes, NJ, USA). Finally ...
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bioRxiv - Immunology 2021Quote: ... IgA and IgM antibodies were quantified in the CVL specimens using the human immunoglobulin cytokine bead array (CBA) flex set assay (BD Biosciences, San Jose, CA, USA). The Ig CBA assay was performed as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with multiple flow cytometry validated antibody cocktails (see below) and analyzed using either BD Fortessa or LSRII cytometers (BD Horizon, BD, Franklin Lakes, NJ). Data were analyzed and quantified using FlowJo v10.6 software (RRID:SCR_008520) ...
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bioRxiv - Genetics 2022Quote: ... The proportion of HbF-and HbS-positive RBCs were measured with an antibody recognizing HbF (Thermo Fisher for HUDEP-2 cells or BD Biosciences for primary cells) or HbS (BioMedomics) ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 × 106 cell/mL cells were stained with the appropriate antibodies and analyzed by flow cytometry (BD FACS Fortessa, BD Biosciences, San Jose, CA, USA). Fluorescence minus one (FMOs ...
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bioRxiv - Microbiology 2020Quote: ... enzyme-linked immunosorbent spot (ELISpot) assays were performed using the Mouse IFN-γ ELISPOT Pair kit including capture and detection antibody (BD Bioscience, Franklin Lakes, NJ, USA) and HRP Streptavidin (BD Bioscience ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were incubated with phycoerythrin (PE)-conjugated Enpep antibody at 4°C for 60 min and then sorted by FACScan flow cytometry (Becton, Dickinson and company, Franklin Lakes, NJ).
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with primary antibody solutions for 5 days at 4°C: mouse monoclonal antiNMDAR1 (GluN1) (1:200; 556308, PharMingen, Becton, Dickinson and Company, Frankline Lakes, USA) or rabbit polyclonal antiNMDAa1 (GluN2A ...
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bioRxiv - Immunology 2021Quote: ... Single-cell suspensions were incubated for 20 min at 4°C with anti–mouse FcγRIII/II (2.4G2; BD Pharmingen) prior to staining for 1 h at 4°C with the following antibodies from BioLegend (most ...
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bioRxiv - Cell Biology 2020Quote: ... and FACS on a BD FACSAriaTM II using the following markers/staining: anti-CD3 PE (clone UCHT1; BD Bioscience), anti-CD4 BV605 (clone OKT4 ...
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bioRxiv - Bioengineering 2022Quote: Cells were fixed in 2% PFA for 15 minutes and incubated with mouse anti-human VE-Cadherin (BD Sciences) at 4°C overnight ...
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bioRxiv - Immunology 2022Quote: ... anti-Ly6C (in PerCPCy5.5, clone HK1.4 from BioLegend Cat# 128012 or clone AL-21 from BD Biosciences Cat# 560525); anti-Ly6G (clone 1A8 in AF700 ...
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bioRxiv - Immunology 2022Quote: Flow cytometry of peritoneal cells was performed using anti-mouse CD16/32 (Fc Block, BD Biosciences, San Jose, CA) before staining with primary antibody or isotype controls ...
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bioRxiv - Microbiology 2019Quote: ... 0.02% NaN3 in PBS) and stained with a FITC-conjugated anti-human HLA-DR mAb (Clone Tü36; BD Biosciences). This antibody specifically binds to a monomorphic epitope of the HLA-DR αβ complex and not the isolated α or β chains or the HLA-DP and -DQ isotypes41 ...
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bioRxiv - Bioengineering 2019Quote: ... The cells dissolved in 100 µl HBSS with 5 µl of PE Mouse anti-E-Cadherin (562526, BD Pharmingen) were incubated for 1 hour ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells from mouse tissues were incubated with nonspecific binding blocker (anti-mouse/human CD16/CD32 Fcγ receptor; BD Biosciences) before staining for V450-human CD45 (BD Biosciences # 560368) ...
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bioRxiv - Cancer Biology 2019Quote: ... incubated in PBS-0.5% BSA containing mouse anti-human Ki-67 (Alexa Fluor 647, clone B56, #558615, BD Pharmingen) for 30 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Immunofluorescence staining for B cells (APC-conjugated rat anti-mouse CD45R/B220, clone RA3-6B2, BD Biosciences, RRID: AB_627078), T cells (CD3-ε ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated for 72 h at 4°C in mouse anti-PKCδ (1:1000, BD Biosciences, 610397), rabbit anti-Phospho-c-Fos (1:2000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells from mouse tissues were incubated with nonspecific binding blocker (anti-mouse/human CD16/CD32 Fcy receptor, BD Biosciences) before staining for APC-human CD33 (Biolegend #303408) ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular staining was performed using rat anti-mouse IFN-γ-PECy7 (clone XMG1-2, 1:300, BD cat#557649), rat anti-mouse IL-2-PE (clone JES6-5H4 ...
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bioRxiv - Cancer Biology 2020Quote: ... 3×103 cells were resuspended in 30 μl PBS + 0.1% BSA and incubated with APC mouse anti-human CD44 (cat.no 559942, BD Bioscience) and FITC mouse anti-human CD24 (cat.no 555427 ...
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bioRxiv - Cancer Biology 2021Quote: ... at 1:200 dilution and 20 µl per sample of anti-cytokeratin conjugated with FITC (BD Pharmingen, Clone CAM5.2) for 45 min ...
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bioRxiv - Bioengineering 2022Quote: ... and anti-hJagged2 PE) for 1 hour at 4°C and analyzed by flow cytometry (BD Accuri C6 plus); however ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation experiments were performed by incubating 100 μg of protein with 2.5 μg of anti-RIPK1 (BD Transduction Laboratories) with rotation at 4 °C for 48 h ...
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bioRxiv - Immunology 2022Quote: ... followed by intracellular staining of the cells with anti-CD3 BV605 (eBioscience, clone OKT3) or BUV661 (BD, clone UCHT1), anti-CD4 BUV737 (BD ...
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bioRxiv - Immunology 2020Quote: ... The following directly conjugated monoclonal antibdodies were used to detect activation markers: anti-CD69–BUV737 (clone FN50; BD Biosciences) and anti-4-1BB–BV421 (clone 4B41 ...
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bioRxiv - Cancer Biology 2020Quote: ... single-cell suspensions of tumors were blocked with purified rat anti-mouse CD16/CD32 (BD Pharmingen, dilution 1:100) for 10 minutes at room temperature then stained with Live/Dead dye (Zombie Aqua ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were detached using TrypLE and resuspended in 1 mL of binding buffer (HEPES-buffered PBS supplemented with 2.5 mM calcium chloride) containing anti-annexin V-APC (BD) and incubated for 15 min in the dark at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Surface staining of cell suspensions was performed by treating the cells with anti-CD16/32 (clone 24G2; BD Biosciences), followed by staining with these antibodies ...
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bioRxiv - Immunology 2022Quote: ... Also added to the culture with the CTLs was anti-CD107a-PE (1:20, BD, or 1:200, Biolegend), GolgiStop (1:800 ...
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bioRxiv - Immunology 2022Quote: ... SiglecF+) as described in Figure S7: anti-CD45-Brilliant Violet 605 (2 µg/ml, clone 30-F11; BD Bioscience), anti-CD11b APC-Cy7 (1 µg/ml ...
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bioRxiv - Immunology 2019Quote: ... For human B cell blots a 1:250 dilution mouse anti-human HIF-1α (BD Biosciences, San Diego, CA) or for mouse blots a 1:100 dilution goat anti-mouse HIF-1α wsa added and the blots incubated O/N at 4 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... or CD31) and E-selectin was measured by flow cytometry using anti-mouse PECAM-1 (Clone 390, BD Biosciences) and anti-mouse E-selectin (Clone 10E9.6 ...
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bioRxiv - Molecular Biology 2020Quote: ... 48h after transfection cells were fixed with PFA 4% and incubated with anti-CD31 (1:40, BD Biosciences 550389) or anti-VE-Cadherin (1:400 ...
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bioRxiv - Immunology 2020Quote: ... CXCR5 was stained with biotinylated anti-CXCR5 (2G8) followed by staining with streptavidin-conjugated PE (both from BD Biosciences). Cell viability was assessed using the Fixable Dye Ghost 510 (Tonbo Bioscience ...
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bioRxiv - Immunology 2020Quote: ... or unconjugated G4 followed by PE-conjugated mouse anti-hamster IgG cocktail (clones G94-90.5 and G70-204; BD). All cells were then analyzed on an LSR II (BD Life Sciences ...
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bioRxiv - Immunology 2020Quote: Cells from mouse BM or spleen were stained with Ab cocktail containing anti-(CD3, B220, NK1.1, CD115, Ly6C) Ab and subjected to single-cell sorting by BD FACS Aria24 ...
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bioRxiv - Immunology 2020Quote: ... for 45 minutes at 4°C in FACS buffer containing 0.5 mg/mL anti-mouse CD16/CD23 (2.4G2) (BD Biosciences). Flow cytometry was performed on an LSR-II with BD FACSDIVA software (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: PBMCs (1−2×106) were stimulated with epitope-specific peptides (5 μM) and anti-CD28 mAb (0.5 μg/mL, BD) and expanded for 14 days in complete RPMI culture medium containing rIL2 (20 IU/mL ...
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bioRxiv - Immunology 2020Quote: ... Cells were seeded (30,000-50,000/wells) into wells pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) along with soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... The viability and purity of the neutrophils were determined by anti-human CD66b PE (clone G10F5; BD Biosciences, 561650) and anti-human CD16 FITC (clone 3G8 ...