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Citations for Becton, Dickinson and Company :
9451 - 9500 of 9716 citations for Anti PIF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... in DPBS for 30 min at room temperature and stained with anti-BrdU-488 (1:50, #51-23614L, BD Pharming) and HOECHST (1:500 ...
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bioRxiv - Immunology 2023Quote: ... rat anti-mouse Ly-6G in BV421™ at 1:300 for 30 minutes (BD Horizon™, Cat. No. 562737), and finally ...
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bioRxiv - Systems Biology 2023Quote: ... Compensation was carried out by generating a matrix using compensation beads (BD™ CompBead Plus; Anti-79 mouse Ig, κ). Cell analysis was carried out on a CytoFLEX S flow cytometer (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were incubated for 15 minutes at 4°C with anti CD16/CD32 Mouse BD Fc Block diluted 1/50 (BD) in 100 µL of FACS buffer in 96 wells round-bottom plates (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... 50 μL of the stain cocktail (1x Brillant Stain Buffer [BD Bioscience] and either 5 μL per sample of Alexa Flour 647 Anti-Total Stat1 [N-Terminus] [BD Biosciences] and 5 μL per sample of PE anti-STAT1 Phospho [Tyr701] [BioLegend] ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted in blocking buffer at 4 °C over-night and then incubated for 30 minutes at room temperature with anti-rabbit IgG coupled with FITC (BD). After washing ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 2 µg/mL of anti-mouse IgE (553413, clone R35-72, BD Pharmingen) in 0.1 M sodium carbonate buffer pH 9.5 ...
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bioRxiv - Immunology 2023Quote: ... Staining was quenched by washing in FACS buffer (PBS with 2 mM EDTA and 2% FCS) after which cells were incubated with anti-CD16/32 (Fc block, clone 2.4G2, BD Biosciences) for 15 minutes at 4°C to prevent non-specific binding ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were resuspended to 50 μL in FACS buffer containing 6.24 μg/mL anti-IgM FITC (G20–127, BD Biosciences), 2.5 μL anti-CD19 BUV395 (SJ25C1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was Fc blocked before staining using 50 µL of 2X FC Block Solution (FACS Buffer, 1:125 dilution of anti-CD16/CD32 (BD) and incubated for 10 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... and tumour-infiltrating myeloid cells (Live CD45+TER119-Kaede+CD11b+NK1.1-) from anti-PD-L1 or isotype-control treated tumours were sorted with a FACS Aria II Cell Sorter (BD) into two groups per cell type ...
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bioRxiv - Neuroscience 2023Quote: ... All dissociated cells were blocked with Fc receptors staining buffer (1% anti-mouse CD16/CD32, 2.4 G2, 2% FBS, 5% NRS, and 2% NMS in HBSS; BD Bioscience) and then stained with a standard panel of antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with a serial dilution from 10 to 0.0006 µg/ml of either anti-CD81 (JS-81; 555675, BD Pharmingen), mouse IgG1 κ isotype control (107.3 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were diluted in 3% BSA/PBST (0.1% Triton X-100 in PBS) and incubated with the samples at 37°C for 1 h: mouse anti-BrdU (BD Biosciences 347580 ...
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bioRxiv - Immunology 2023Quote: ... high-binding 96-well plates were coated with 5 μg/ml anti-CD3 (Clone UCHT1, BD Pharmingen™ #555329, USA) in PBS (ThermoFisher Gibco™ #21600044 ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse monoclonal anti β2-spectrin (against residues 2101–2189 of human β2-spectrin, 1:100 for IF) was from BD Biosciences (#612563) ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... Anti-TCR Vα2-PE (# 553289), anti-CD8α-BUV395 (# 563786), Stain Buffer (FBS, # 554656) and BD Cytofix/Cytoperm kit (# 554714) were purchased from BD Biosciences ...
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bioRxiv - Physiology 2024Quote: ... Cells were then pelleted at 500 x g for 3 min at 4°C and resuspended in 25 μL of 5 mg/mL FcBlock (rat anti-mouse CD16/32, clone 2.4G2, BD Biosciences) diluted in FACS Buffer and incubated on ice for 10-15 min ...
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bioRxiv - Physiology 2024Quote: ... The sections were incubated for 1 h at room temperature in 0.3% BSA in PBS with a rat anti-mouse Ly6G (BD Bioscience), a rat anti-MOMA-2 (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... and labeled overnight at 4°C in 1% BSA in PBST containing mouse anti-G3BP1 (BD Biosciences, 1:1000 dilution) and rabbit anti-Caprin-1 (Proteintech ...
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bioRxiv - Immunology 2024Quote: ... Purity > 97% of CD14+ monocytes after magnetic sorting was verified by flow cytometry (anti-CD14-FITC clone MφP9, 1:40, #345784; BD Biosciences). For macrophage differentiation ...
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Inhibition of CELA1 Improves Septation in the Mouse Hyperoxia Model of Impaired Alveolar DevelopmentbioRxiv - Developmental Biology 2024Quote: One million cells from each lung were incubated (4°C, 30 minutes) with anti–mouse CD16/CD32 (BD Biosciences, 553142) to block Fc receptors ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice with PBS and incubated for 20 minutes on ice with 1:100 dilution anti-CD16/32 (clone 2.4G2, BD biosciences) and 1:500 dilution Live/Dead nearIR dye (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibodies for immunostaining were diluted in PBS with 3% BSA and 0.1% Tween-20 as follows: 1:800 rat anti-mouse CD31 (BD, 553370), 1:1000 rabbit anti-ERG (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% bovine serum antigen) and stained with either 1:20 anti-HLA-A,B,C-PE (clone W6/32, BD Biosciences), 1:100 anti-β2m-FITC (clone 2M2 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined by BCA assay and 250 µg protein lysates were incubated with anti-Myc (2.5 µg, BD Pharmingen 551102) or anti-IgG (2.5 µg ...
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bioRxiv - Immunology 2021Quote: ... MLN cell suspensions were added to a 96 well plate for surface staining with anti-mouse TCRβ-FITC (clone H57-597, BD Biosciences) and anti-CD4-PerCP-Cy5.5 (clone RM4-5 ...
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bioRxiv - Immunology 2021Quote: ... then sorted GFP+ cells were expanded and assessed for CD47 expression by staining with BV786-conjugated anti-hCD47 mAb B6H12 (BD Bioscience) and PE-conjugated anti-hCD47 mAb CC2C6 (Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... and surface stained for 30 mins at 4°C with 50 μL/well anti-mouse CD3 APC-CY7 (clone 17A2, 1:400, BD Biosciences), anti-mouse CD4 BV510 (clone RM4-5 ...
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bioRxiv - Molecular Biology 2021Quote: ... run on PAGE together with input sample (1:20 of amount of immunoprecipitated proteins) and blotted with anti-PARP1 (Mouse Mab 551025 BD Pharmigen), anti-TRF1 (Rabbit Pab sc-6165 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were re-suspended in 100 μl of FCSB and 1 μl of mouse anti-human CD10-PE (561002, BD Biosciences) and mouse anti-human CD92-AF647 (565316 ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... and with hematopoietic stem cell markers APC-conjugated anti-CD117 (c-Kit) and PE-conjugated Ly6A/E (Sca-1) (BD Biosciences). Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C with the astrocyte (mouse anti-GFAP; 1:1000, BD Biosciences, San Diego, CA, USA) and microglia (rat anti-CD11b ...
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bioRxiv - Immunology 2022Quote: ... anti-CD45 (clone 30-F11 in PECy7 from eBiosciences Cat# 25-0451-82 or in BV786, from BD Biosciences Cat# 564225); anti-CD45.1 (clone A20 in APC from BioLegend Cat# 110714) ...
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bioRxiv - Immunology 2022Quote: ... NK cells were added to the washed ELISA plate and incubated together with anti-human CD107a (BD, 1:40, clone: H4A3), brefeldin A (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... stained with anti-CD117-PECy7 mAb (5 μg.mL−1) and viability dye and sorted by a FACSMelody™ cell sorter (BD biosciences). For immunofluorescence experiments ...
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bioRxiv - Immunology 2020Quote: ... then cultured in complete RPMI media (3×106 cells/mL) plated on anti-CD3 (145-2C11; BD Pharmingen, San Diego, CA) antibody-coated tissue culture plates ...
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bioRxiv - Cell Biology 2019Quote: ... Tet1KO and WT reprogramming intermediates were incubated 30 min on ice with Phycoerythrin (PE)-conjugated Mouse anti SSEA1 (1:100, Cat.#FAB2155P-025, R&D) and analysed on CANTO I (BD Biosciences). PGCLCs at day8 were sorted using FITC Mouse anti-SSEA1 (1:20 ...
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bioRxiv - Immunology 2019Quote: Lymphocytes or purified splenic B cells were washed in PBS with 1% BSA and incubated with 1mg/ml purified rat anti-mouse CD16/CD32 (Fc block, BD Pharmingen) at 4°C for 15 min ...
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bioRxiv - Immunology 2019Quote: ... incubated with anti-mouse IgG1-fluorescein isothiocyanate for 45 min at room temperature (1:100 dilution; BD Pharmingen; cat. no. 553443), and washed three times with PBS ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell suspensions from APEL vascular differentiations were incubated with mouse anti-human CD31-APC (eBioscience, San Diego, CA) and CD146-PE (BD Biosciences) antibodies for 30 min on ice ...
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bioRxiv - Microbiology 2019Quote: ... the cells were washed once with FACScan buffer and then stained with PE-conjugated monoclonal rat anti-mouse CD4 (BD Pharmingen) and/or APC-conjugated monoclonal rat anti-mouse CD8 (eBioscience) ...
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bioRxiv - Immunology 2019Quote: ... B cells were pre-incubated during 15 minutes with 0.5 μg/ml anti-mouse CD16/CD32 (Clone 2.4G2, BD Pharmingen, ref 553142) in FACS buffer (PBS supplemented with 2% FCS and 2 mM (Ethylenedinitrilo)tetraacetic acid (EDTA ...
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bioRxiv - Immunology 2019Quote: ... IgM-negative B cells were purified from lymphocytes using biotinylated anti-rat IgM (Clone G53-238, BD Biosciences, San Jose, CA) and magnetic separation (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2019Quote: ... cKIT and PDGFRA expression was assessed using mouse anti-human CD117 (PE-Cyanine7, clone 104D2, #25-1178-42, eBioscience) and CD140a (PE, clone αR1, #556002, BD Pharmingen) respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then stained with anti-IgA-PE and the fluorescence signal was acquired on an LSR II or Fortessa X-20 flow cytometer (BD Biosciences). AID protein was detected by immunoblotting with mouse mAb L7E7 (Cell Signaling #4975 ...
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bioRxiv - Cancer Biology 2019Quote: ... were stained with monoclonal rat anti-BrdU (1:500) (Clone BU1/75 (ICR1) (AbD Serotec) and monoclonal mouse anti-BrdU (1:500) (Clone B44, 347580 (BD Biosciences)) respectively for 1 hour in 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed and permeabilised cells were washed with PIPES buffer and stained with mouse anti-e-cadherin (Clone 36/E; BD Bioscience) in 5% goat serum and 0.1% saponin containing PIPES buffer for 1 h washed and stained with secondary antibodies coupled to Alexa 647 for 45 min ...
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bioRxiv - Immunology 2021Quote: ... labelled with anti-human myeloid and stromal markers (Table 1) and analysed by flow cytometry on a LSR Fortessa (BD Biosciences).
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bioRxiv - Neuroscience 2020Quote: ... The pellet was recovered and sequentially incubated with anti-mouse CD16/CD32 (1:250, BD Pharmingen; for 15min at 4°C) followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200 ...