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Citations for Becton, Dickinson and Company :
9151 - 9200 of 9716 citations for Anti PIF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used in the cocktail for surface staining: CD3-allophycocyanin (APC)-Cy7 (clone SP34-2, Cat#557757, BD Pharmingen, San Jose, CA), CD4-PE-CF594 (clone L200 ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... cells were centrifuged and resulting pellets were resuspended in 1:100 mixture of panel of antibodies: FITC-conjugated CD31 (BD Biosciences, Mississauga, ON, Canada), AF647-conjugated CD45 (Southern Biotech ...
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs were assessed to determine the frequency of circulating T follicular helper (Tfh) using a panel which included the following antibodies: CD3 (BD Biosciences; clone SP34-2), CD4 (BD Biosciences ...
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bioRxiv - Physiology 2021Quote: ... A 3% BSA-0.2% Triton®X-100 solution was used for permeabilization and blocking followed by a 1 h RT incubation with PBS–1% BSA containing mouse monoclonal DRP1 antibody (611113, BD Transduction Laboratories, 1:50) followed by a 1 h RT incubation with PBS–1%BSA containing rabbit polyclonal RyR2 (PA5-36121 ...
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bioRxiv - Immunology 2021Quote: ... harvested and stained for flow cytometry as described (37) using antibodies to human surface antigens: CD4-APCH7 (SK3, BD Biosciences Franklin Lakes, NJ, USA), CD8-PerCP (SK1 ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were blocked in 5% BSA and probed overnight at 4°C with the following primary antibodies and dilutions: p16 (1:200, #554079 BD Biosciences, San Jose, CA), p53 (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and then resuspended in 100 μl of staining solution of FACS buffer containing biotinylated antibodies against CD31 (BD Biosciences, 558737, 1:100), CD45 (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions containing 3×106 cells/sample were stained using appropriate fluorescent antibody cocktails (Listed in Supplementary Table S1) after Fc blocking and analyzed using flow cytometry (BD FACS Celesta/Novocyte Advanteon). Cells were fixed and permeabilized using intracellular staining reagents (Intracellular Fixation & Permeabilization Buffer Set ...
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bioRxiv - Immunology 2024Quote: ... The samples included for T cell phenotypic characterization were stained with a separate antibody cocktail containing T cell lineage markers (CD3-BV786 (BD, cat. #563799, clone SK7), CD4-BV650 (BD ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by incubation with primary antibodies for at least 1 hr at room temperature and secondary antibodies (for nonconjugated primary antibodies) for 30 min at room temperature with washes between the steps before analysis by flow cytometry (BD Fortessa or MACSQuant 16). To exclude dead cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with fluoresceinated antibodies against (TCRý, CD4, CD8, CD3, CD3χ, CXCR5, PD1, and intracellularly for ZAP70 and IL-10) (BD Biosciences, San Jose, CA) and data acquired on a FORTESSA flow cytometer and analyzed using FlowJo Software (Ashland ...
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bioRxiv - Cell Biology 2023Quote: ... density centrifugation or 1X RBC Lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with monoclonal antibodies conjugated to fluorescent molecules as listed in Supplemental Table 5 after treatment with Fc Block (BD Biosciences, Franklin Lakes, NJ). Cells were analyzed on either a BD LSR-II or BD LSRFortessa X-20 flow cytometer running BD FACSDiva software or a Cytek Aurora spectral cytometer running SpectroFlo software and analyzed using FlowJo software (TreeStar).
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked by incubation in 5% non-fat milk powder in 0.1% Tween 20 in Tris buffered saline (TBS-T) and incubated overnight at 4°C with primary antibodies: ALIX (BD Biosciences, 611620, 1:1000), calnexin (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... 1x106 cells in 100 μL were stained for 30 minutes at room temperature using the following antibodies: CD90-BUV395 (BD, #740260, 0.1 mg/μL), ECAD-AF405 (Novus Bio ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was blocked in 5% non-fat dry milk in TBS/Tw and then incubated overnight with primary antibody Syn-1 (BD Biosciences #610,787, 1:1000) or MJFR-14-6-4-2 (Abcam #209,538 ...
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bioRxiv - Genetics 2023Quote: ... were isolated by 1X Red Blood Cell lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Microbiology 2023Quote: Surface markers of splenocytes were identified using monoclonal antibodies in conjunction with two immunofluorescence analysis with a flow cytometer (BD Accuri C6 Plus: BD, Mountain View, CA, USA). Antibodies used were Fluoroscein isothiocyanate (FITC)-conjugated anti-mouse CD4 and CD21/CD35 (CR2/CR1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary incubations were completed in blocking buffer using Alexa Fluor 488 Mouse anti-SOX2 (BD Pharmingen, O30-678) and Alexa Fluor® 647 Mouse anti-CDX-2 (BD Pharmingen ...
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bioRxiv - Cell Biology 2020Quote: ... Immune and endothelial cells were excluded using anti-CD45 (Alexa Flour 700; Cat#560510; BD; used 1:200) and anti-CD31 (APC/Fire750 ...
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bioRxiv - Pathology 2022Quote: ... Murine Fc receptors were blocked using anti-mouse CD16/CD32 (FcBlock clone 2.4G2, BD Biosciences, #553141, 1/200) and cells were stained in FACS buffer for 30 min at 4°C with the following anti-mouse antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with MACS buffer and incubated with anti-PE magnetic beads (BD Biosciences, San Jose, CA) at a concentration of 25µl/10 million cells ...
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bioRxiv - Immunology 2022Quote: ... anti-B220/CD45R (clone RA3-6B2 in APCeF780 from eBiosciences Cat# 47-0452-82, in AF700 from BD Biosciences Cat# 557957 or in FITC ...
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bioRxiv - Genomics 2020Quote: ... Staining was performed overnight at 4°C with anti-Pax6-A488 (1:200, BD Biosciences, Cat. N: 561664), anti-Eomes-PE (1:200 ...
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bioRxiv - Immunology 2021Quote: ... IL-1β was detected using biotinylated anti IL-1β (eBioscience) and streptavidin conjugated to horseradish peroxidase (BD Biosciences). Peroxidase activity was detected using an o-phenylenediamine hydrochloride (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: ... Sections were stained at 4°C overnight with primary rat anti-mouse CD11b-FITC (M1/80; BD Bioscience) and rat anti-mouse Ly6G-FITC (1A8 ...
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bioRxiv - Cancer Biology 2019Quote: ... Naïve CD8+ T cells from C57BL/6 splenocytes were sorted using anti-mouse CD8a particles (BD Biosciences, 551516) and cultured under CD3 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were labeled with APC-conjugated mouse anti-human CD44 (1:20; G44-26; BD, Franklin Lakes, NJ), APC-conjugated mouse anti-human EPCAM (1:20 ...
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bioRxiv - Immunology 2021Quote: ... Compensation controls were prepared using compensation beads (Anti-mouse Ig,K Neg Control compensation, BD® cat. 552843) following manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... the cell suspension was transferred to a petri dish pre-coated with anti-PDGFRα (BD Pharmigen 558774; RRID:AB_397117) and the OPCs allowed to adhere for 45 min at 21°C ...
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bioRxiv - Neuroscience 2020Quote: ... the cells were washed and labelled with a cocktail containing anti-human CD11b-PE (clone D12; BD Biosciences) and anti-human CD14-PECy7 (clone M5E2 ...
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bioRxiv - Biochemistry 2020Quote: ... mouse monoclonal anti-EEA1 (1:500, clone 14, Cat #610457, RRID: AB_397830, BD Biosciences, San Hose, CA, USA), rabbit polyclonal anti-EEA1 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... The cells were blocked by adding 5µg/ml purified rat anti-mouse CD16/CD32 (mouse BD Fc Block) clone ...
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bioRxiv - Immunology 2021Quote: ... Degranulation activity of the NK cells is then measured by FACS by the anti-CD107a (FITC BD Biosciences) and IFN-γ staining (PE BD Biosciences).
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bioRxiv - Neuroscience 2020Quote: H4 protein extracts were analyzed by western-blotting probing for aSyn (anti-a-synuclein dilution 1:1000; BD Transduction Laboratories™ ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Myosin7a (MYO7; 1:200; Proteus; RRID:AB_10013626) mouse anti-CTBP2 (aka C-Terminal Binding Protein 2; 1:200; BD Transduction Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained with PE-conjugated anti-human CD33 or isotype control mAbs (BD Pharrmingen, San Jose, CA).
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bioRxiv - Cancer Biology 2020Quote: ... Brain microvessels were stained overnight using a rat anti-mouse PECAM-1/CD31 (1:400, 553370, BD Pharmingen). Cell nuclei were counterstained with DAPI (1 μg/mL ...
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bioRxiv - Microbiology 2022Quote: ... Up to 5ml of venous blood was collected in heparinised anti-coagulation BD Vacutainer® tubes (BD Biosciences), and a proportion of the erythrocytes were cryopreserved in glycerolyte and stored at −80°C or in liquid nitrogen before shipment on dry ice to the London School of Hygiene and Tropical Medicine for subsequent culture and laboratory analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 µg/ml of a NA/LE hamster anti-mouse CD29 clone HM β1-1 (562219; BD Bioscience), and 5 µg/ml of a NA/LE hamster IgG2 (553961 ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was incubated overnight at 4°C with mouse anti-LR11 (1:500, #612633, BD Transduction Laboratories) for SORLA detection ...
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bioRxiv - Immunology 2020Quote: ... Purity of MHCII-depleted splenocytes was analyzed by anti-CD3 (Clone 145-2C11, BD Biosciences, New Jersey, USA) and anti-CD19 (Clone 1D3 ...
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bioRxiv - Immunology 2020Quote: ... MDA-MB-231 and MCF7 cells were stained with PE-labeled anti-MICA/B (clone 6D4, BD Pharmingen) and PE-Cy5-labeled anti-HLA-ABC (G46-2.6 ...
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bioRxiv - Immunology 2019Quote: ... SIVmac239 Gag Peptide Set #12364) in the presence of anti-CD28 (1 μg/mL, clone L293, BD Biosciences) and anti-CD49d (1 μg/mL ...
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bioRxiv - Developmental Biology 2020Quote: ... resuspended and incubated for 30min at 4C with APC rat-anti-mouse CD31 (BD Pharmigen, 551262, 1:200). Cells were then centrifuged (300g ...
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bioRxiv - Microbiology 2020Quote: ... N or M peptide pools (10μg/ml) in the presence of anti-CD28 (1μg/ml; BD Biosciences, USA) and recombinant interleukin (IL)-2 (20U/ml ...
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bioRxiv - Molecular Biology 2019Quote: ... Chemerin was detected using biotin-conjugated rat-anti mouse chemerin mAb (BAM2325) followed by streptavidin-HRP (BD Science). The reaction was developed with TMB substrate (BD Science) ...
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bioRxiv - Cancer Biology 2021Quote: ... Fc receptors were blocked with purified rat anti-mouse CD16/CD32 (Mouse BD Fc Block, BD Biosciences, CA). Anti-mouse antibodies were used to characterize the lymphoid populations ...
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bioRxiv - Cancer Biology 2021Quote: ... Fc receptors were blocked with purified rat anti-mouse CD16/CD32 (Mouse BD Fc Block, BD Biosciences, CA). Anti-mouse antibodies were used to characterize the lymphoid populations ...
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bioRxiv - Immunology 2021Quote: ... and the universal T cell surface marker CD3 V450 (Mouse Anti-Human CD3, BD Biosciences; catalogue number: 560365) was used at a concentration of 5μL in a 50µL final staining volume per section ...