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Citations for Becton, Dickinson and Company :
5651 - 5700 of 5734 citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and the cell pellet was stained with PBS containing 1:1000 diluted BD Fixable Viability Stain 780 (Cat. # 565388, BD Biosciences, Heidelberg, Germany) for 15 minutes at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed at least three times with 1×PBS (pH 7.4) before flow cytometric analysis using BD Accuri C6 (BD Biosciences, San Jose, USA) with FITC channel.
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bioRxiv - Microbiology 2024Quote: Water samples prepared as previously described [35] and sediment (1 g) were enriched in 10 ml of buffered peptone water (BPW; BD DifcoTM, Becton Dickinson) or E ...
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bioRxiv - Neuroscience 2024Quote: ... The cover slips were then permeabilized with 0.3% Triton X-100 and sequentially stained with rat anti-CD44 antibody (BD Biosciences, 550538; 1:200), and corresponding Alexa 488-conjugated secondary antibodies (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... slides were processed as described previously for section immunofluorescent staining for detection of mouse IgG2a anti-E-cadherin (1:500, BD Biosciences, Cat# 610181) and imaged on a Leica SP5 upright confocal ...
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bioRxiv - Immunology 2021Quote: Treatments comprising 15μl of EVs suspension in PBS corresponding to EV secretions from ~5.0 × 105 cells or PBS alone controls were injected intra-articularly 1 day post arthritis induction with 0.5ml monoject (29G) insulin syringes (BD Micro-Fine, Franklyn Lakes, USA) through the patellar ligament into the right knee joint ...
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bioRxiv - Neuroscience 2021Quote: ... Stimulated cells were stained with fluorochrome-conjugated anti-MCP-1 and anti-IL-6 Abs using the Cytofix/Cytoperm kit (BD Biosciences, San Jose, CA) to determine MCP-1 and IL-6 production ...
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bioRxiv - Microbiology 2019Quote: ... ESAT6 peptide (1 μg/ml) or TB10 peptide (1 μg/ml) for 24 hours at 37°C before the addition of GolgiPlug (BD Pharmingen, San Diego, CA). The concentration of protein and peptides used were according to previous reports [19 ...
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bioRxiv - Microbiology 2021Quote: ... and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution, BD Pharmingen, cat No. 559864). Cell nucleus was stained with DAPI ...
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bioRxiv - Neuroscience 2021Quote: ... was sprayed on the plantar surface of the hind right paw using a syringe of 1 ml (Tuberculin slip tip, BD, Franklin Lake, NJ, USA). Paw nociceptive responses ...
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bioRxiv - Systems Biology 2020Quote: ... HL60 and U937 cells (1 mL) were immediately fixed by addition of 0.2 mL of x5 Lyse/Fix buffer (BD Biosciences, San Jose, CA, USA) for 10 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cochlear whole mounts were immunostained overnight at 4°C with primary antibody against CTBP2 (mouse anti-CTBP2 at 1:200; BD Transduction Labs, Heidelberg, Germany). Secondary antibody (donkey anti mouse conjugated with Cy3 ...
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bioRxiv - Microbiology 2021Quote: Detection of extracellular nectin-1 in infected cells was performed using a BD LSR II or BD LSR Fortessa cytometer (BD Biosciences, San Jose, CA). Infected cells were harvested by 5mM EDTA supplemented with Dulbecco’s PBS (D-PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated overnight at 4°C in solution containing 1% BSA and primary antibodies: mouse F4/80 (T45-2342, BD Biosciences, New Jersey, USA) 1:200 ...
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bioRxiv - Systems Biology 2021Quote: ... xanthus strains were grown overnight at 32°C with vigorous shaking in CTTYE broth (1% Casein Peptone [Remel], 0.5% Bacto Yeast Extract [BD Biosciences], 10mM Tris (pH 8.0), 1mM KH(H2)PO4 ...
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bioRxiv - Microbiology 2021Quote: ... were anesthetized and one 1-cm long catheter segment inserted into the peritoneum using an 18-gauge BD Spinal needle/stylette (Becton, Dickinson and Company, USA). Four hrs post-catheter implantation ...
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bioRxiv - Cancer Biology 2020Quote: Cells were treated as indicated and stained with fluorescent conjugates of annexin-V and PI (1 µg/ml final concentration) and analyzed on a FACSCanto machine (BD, Franklin Lakes, NJ, USA). Annexin V was prepared as previously described (Brumatti et al. ...
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bioRxiv - Biophysics 2022Quote: ... Cell suspensions were homogenized by passing 10 times through a precooled 1-ml syringe with a 27-gauge stainless steel needle (BD Biosciences, Franklin Lakes, NJ). The homogenate was adjusted to about 40% sucrose by the addition of 700μl of 60% sucrose prepared in TNE and placed at the bottom of an ultracentrifuge tube ...
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bioRxiv - Cancer Biology 2022Quote: qNSCs and aNSCs from the SVZ were FACS purified from GFAP::GFP mice as previously described (Codega et al., 2014) using the following antibodies: anti-mCD24 PE (1:2000; BD Pharmingen 12-0242-82), biotin conjugated anti-mCD133 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA, and BD OptEIA Substrate Solution, BD Biosciences, for Spike ELISA) for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... The transfer of the retinal suspension onto panning plates bound with monoclonal mouse anti-SSEA-1 (CD15)(MC480 clone) (#BD560079; BD Pharmingen, San Jose, CA) and monoclonal mouse anti-HNK-1/N-CAM (CD57)(VC1.1 clone ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA,and BD OptEIA Substrate Solution, BD Biosciences, for Spike ELISA) for 5 min ...
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bioRxiv - Immunology 2022Quote: Total white blood cells from 1-2 mL freshly collected blood in EDTA tubes were enriched through ammonium chloride lysis (BD Bioscience, catalog no. 555899), followed by centrifugation (300x g for 10min) ...
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bioRxiv - Cell Biology 2019Quote: ... The following day they were probed with antibodies to FLAP (Novus, IMG 3160, 1:100) and to 5-LO (BD Biosciences, 610695, RRID: AB_398018). Secondary antibodies were applied at 3 µg/mL for 1 h ...
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bioRxiv - Physiology 2021Quote: ... Sections were then washed with PBS 3 times for 15 min and incubated with PBS containing Hoechst (1:5000, #561908, BD Pharmingen, Lincoln Park, NJ) for 5 min before a final series of washes with PBS.
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bioRxiv - Microbiology 2021Quote: ... coli BL21 (DE3) strain harboring pCOLD-1-EhPTEN1 was grown at 37°C in 50 ml of LB medium (BD Difco, New Jersey, USA) in the presence of 100 µg/ml ampicillin ...
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bioRxiv - Cancer Biology 2021Quote: ... or 4T1.2 cells (1⨯106 per fat pad) in 50 μL of 50:50 (v/v) PBS:Matrigel™ (BD Biosciences, San Jose, CA, USA). Tumor growth was measured twice weekly by caliper measurements and weekly bioluminescent imaging (125 mg/kg luciferin I.P. ...
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bioRxiv - Microbiology 2019Quote: ... The mixture was filtered (Whatman #1) from which 10mL of the filtrate aliquoted into 14mL BD Falcon™ test tubes (BD Biosciences, Bedford, USA) and labeled accordingly ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... cells were centrifuged and resulting pellets were resuspended in 1:100 mixture of panel of antibodies: FITC-conjugated CD31 (BD Biosciences, Mississauga, ON, Canada), AF647-conjugated CD45 (Southern Biotech ...
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bioRxiv - Cell Biology 2020Quote: ... re-suspended in αMEM (with 10% FBS and 1% anti-mycotic) and then processed on an Aria II or Influx flow cytometer (BD Biosciences, San Jose, CA) to separate tdTomato-expressing (tdTom+ ...
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bioRxiv - Immunology 2020Quote: ... LN staining: macrophages were detected using a primary antibody against CD11b (1/1000, rat anti mouse CD11b conjugated with PE; BD Biosciences, San Jose, California), B-cell follicles were identified using rat anti mouse B220 Alexa F647 (1/1000 ...
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bioRxiv - Genomics 2021Quote: ... FCM were performed on 50 μl sample in triplicate at a pre-set flow rate of 66 μl.min-1 using a BD Accuri® C6 flow cytometer (BD Accuri® cytometers, Belgium) which is equipped with a 50mW solid state laser emitting light at a fixed wavelength of 488 nm ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were blocked in 5% BSA and probed overnight at 4°C with the following primary antibodies and dilutions: p16 (1:200, #554079 BD Biosciences, San Jose, CA), p53 (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and then resuspended in 100 μl of staining solution of FACS buffer containing biotinylated antibodies against CD31 (BD Biosciences, 558737, 1:100), CD45 (BD Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... Membranes were washed 5x times with TBS-T and stained overnight at 4°C with gentle agitation with primary antibodies: mouse anti-rat CD63 (1:200 dilution, BD Biosciences, Mississauga, ON, Canada), rabbit anti-rat CD9 (1:500 dilution ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained with Fixable Viability Dye eFluor 506 (1:1000 in PBS, 65-0866-14) and later CD14-APC (555399, BD Biosciences, Franklin Lakes, USA) and CD16-FITC (555406 ...
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bioRxiv - Genomics 2022Quote: The collected cells were first stained with live/dead aqua to exclude non-viable cells from the analysis and stained with fluorophore-conjugated antibodies against surface markers anti-CD45-BV786 (BD Bioscience Clone: OX-1), anti-CD11b-V450(BD Bioscience Clone ...
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bioRxiv - Neuroscience 2022Quote: ... approximately 1-2 mm apart along the same bundle of muscle fibers with the help of a 20G syringe tip (BD Microlance, Thermo Fisher Scientific). After the dental cement hardened ...
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bioRxiv - Immunology 2022Quote: ... 1.5 µg/mL) [31] in the presence of anti-CD28 and anti-CD49d antibodies (both at 1 μg/ml, BD, Franklin Lakes, New Jersey) and brefeldin-A (10 μg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... The stained cell suspensions were fixed in 4% paraformaldehyde and resuspended in PBS (1% BSA) before flow cytometry analysis using a FACSymphony™ A3 cell analyzer (BD Biosciences, NJ, U.S.A.).
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bioRxiv - Immunology 2024Quote: ... Purified naïve CD4+ T cells were stimulated with 2.5 μg/ml of anti-CD3e (#145-2C11, Cytek Biosciences, Fremont, CA) and 1 μg/ml of anti-CD28 (#553294, BD Biosciences, Franklin Lakes, NJ) antibodies in different culture conditions ...
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bioRxiv - Cell Biology 2024Quote: ... upper membrane parts were first probed with rat monoclonal anti-pSMAD1/9 antibody (pS463/pS465, pS465/pS467, 1:1,000, #562508, BD Biosciences, East Rutherford, NJ, USA), or rabbit monoclonal anti-pSMAD2 (S465/467 ...
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bioRxiv - Evolutionary Biology 2023Quote: Fecal samples of captive great apes from the Columbus and Houston Zoos (Supplemental Table 1) were collected using the BBL™ CultureSwab Collection and Transport System (BD Biosciences, NJ, USA), and shipped overnight at ambient temperature to our research laboratory where they were stored at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... spun for 10 minutes and then resuspended in 50 μL of a 1:200 dilution of Fc block (CD16/CD32, clone 2.4G2, BD Biosciences, cat. 553141, 0.5mg/mL) in FACS buffer (PBS ...
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bioRxiv - Bioengineering 2023Quote: ... so that the microfluidic tubing (Scientific Commodities,Inc., BB31695-PE/3) can connect the device to the sample sources in a syringe (BD 1 mL, NJ, USA).
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: pCAF sorting based on TNFRSF12A protein level: 106 cells were incubated with BD Horizon BV421 anti-TNFRSF12A antibody (1/140, 565712 BD Biosciences, Franklin Lakes, NJ) and human TruStain FcXTM (1/200 ...
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bioRxiv - Developmental Biology 2023Quote: ... before the cell pellet was resuspended in 100µl Fc block (mouse BD Fc Block CD16/CD32 Clone 2.4G2, Cat#: 553141, BD Pharminogen, 1:100 in FACS buffer) and incubated on ice for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were counter-stained with DAPI at a final concentration of 1 ng/mL and analyzed on a BD LSR Fortessa instrument (BD Biosciences, Franklin Lakes, NJ). The resulting data was processed using the software FlowJo (BD Biosciences) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 30 min (RT) and incubated overnight (4 °C) with mouse anti-5-LOX (1:1000; # 610695; Lot: 9067817; BD Biosciences, Franklin Lakes, NJ) and rabbit anti-perilipin-2 (1:100 ...
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bioRxiv - Immunology 2024Quote: ... The following surface antibodies were used at the manufacturer recommended dilution of 0.25 μg antibody per million cells with the exception of CD57 APC which was tittered down 1/5 in order to be on scale: HLA-DR BUV395 (BD Biosciences, Clone: G46-6), NKp46 BV650 (BD Biosciences ...