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Citations for Becton, Dickinson and Company :
5201 - 5250 of 5734 citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed with PBS containing 5 mM EDTA and 3% FBS (FACS Buffer) and then blocked with 1% anti-mouse CD16/CD32 (BD biosciences), 5% normal rat serum and 5% normal mouse serum in FACS buffer for 10 minutes at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Discs were then washed three times for 10 minutes each in 0.1% PBST and incubated overnight at 4°C in mouse anti-BrdU primary antibody (B44) (BD, 1:50). The next day ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins of interest were visualized after incubation with primary antibodies (α-synuclein 1:1000 BD Biosciences (San Jose, CA, USA) #610787 ...
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bioRxiv - Plant Biology 2021Quote: ... and diluted to a final concentration of 1.5 x 105 spores ml−1 in half strength potato dextrose broth (PDB ½, BD Difco™) prior to the inoculation ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were incubated for 15 minutes at room temperature in the dark in 50uL PBS with Fc Block (1/100 dilution, BD, 564220) and Zombie UV dye (1 in 500 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... at 1:1 E:T ratio at 37°C with 5% CO2 in the presence of a CD107a antibody for 1 hr before treatment with brefeldin A (10 µg/ml; BD Biosciences) and monensin (0.7µg/ml ...
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bioRxiv - Microbiology 2020Quote: ... AU) of the infected cells 1 and 3 hours after infection by flow cytometry using a FACS Aria III (BD Biosciences). The intensity of GFP fluorescence corresponds to the amount of intracellular bacteria ...
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bioRxiv - Microbiology 2020Quote: ... red blood cells in a concentration of 1 × 107 cells/mL were incubated for half an hour with 100 μl thiazole orange (TO) (Retic-Count Reagent; BD Biosciences) and Alexa-647 conjugated mouse mAb against transferrin receptor (CD71 ...
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bioRxiv - Microbiology 2020Quote: ... and combined 1:1 with Tris-Borate-EDTA buffer (TBE) at a final working concentration of 1X in 50 mL conical tubes (BD Falcon). Samples (1.0 mL in 50 mL conical tubes ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 72 h at 4°C in mouse anti-PKCδ primary antibody (1:1000, BD Biosciences, 610397) in blocking solution containing 1.5% NGS ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... for 24 h and HIV-1 reactivation was assessed by estimating GFP+ cells (excitation: 488 nm; emission: 510 nm) using BD FACSVerse flow cytometer (BD Biosciences). The data were analysed using FACSuite software (BD Biosciences).
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bioRxiv - Neuroscience 2020Quote: ... The EGFP+stop cassette was inserted in-frame into exon 1 at the AgeI site following amplification from pEGFP-C1 (BD Biosciences) using primers 2-For and 2-Rev ...
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bioRxiv - Immunology 2021Quote: ... GFP-positive transfected cells were sorted in 96-well plates at a density of 1 cell per well using a FACS ARIA III (BD Biosciences) and cultivated in THP-1 media ...
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bioRxiv - Immunology 2021Quote: ... Cells were aliquoted to a count of 1 million cells each and incubated on ice with Fc Block (BD, Table S1) at a 1:20 dilution ...
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bioRxiv - Immunology 2020Quote: ... 8.0 × 105 cells per condition were fixed in 1% PFA for 10 min at room temperature and subsequently stained with primary antibodies: mouse anti-human PEcy7-CD16 (BD #557744), mouse anti-human PerCPcy5.5-CD66b (Biolegend # 305107) ...
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bioRxiv - Genomics 2021Quote: ... the suspension was sorted by applying a 4-way purity mode based on the selected DAPI positive nuclei population (1:1000, BD Pharmingen) using forward and side scatter gating strategy (FACS) ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were run for 5 min at low flow rate (12 ± 3 μl min−1) and cytometric data were documented using BD CellQuest Pro software (BD Biosciences). In addition to glaucophyte cells ...
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bioRxiv - Systems Biology 2020Quote: ... cells were extensively washed with PBS and stained with NeutrAvidin Protein DyLight 488 1:50 for 20 min prior analysis using an Accuri C6 Flow Cytometer (BD Biosciences) with side-scatter (height ...
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bioRxiv - Microbiology 2020Quote: ... during 15 min at 4 °C in MACS buffer followed by incubation with anti-CD45 1:100 (clone 30F-11, BD biosciences), anti-EpCAM 1:100 (clone G8.8 ...
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bioRxiv - Microbiology 2020Quote: ... http://www.eucast.org) and ISO 20776-1:2006 guidelines with the exception that cation-adjusted Mueller-Hinton broth (BD, New Jersey, USA) was supplemented with 100 U of catalase (Worthington Biochemical Corporation ...
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bioRxiv - Cancer Biology 2020Quote: ... For both ACHN and CAKI-1 cell lines 2×106 cells were diluted in 50 µl of PBS and 50 µl of Matrigel (BD Biosciences) and were injected subcutaneously into the right and left flank of each mouse ...
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bioRxiv - Cancer Biology 2020Quote: ... into a 50 mL conical vial using a 10 mL serological pipette and the plunger from a 1 mL syringe (BD, 309628). Cell strainers were washed with 25 mL sterile PBS into corresponding conical vials ...
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bioRxiv - Cancer Biology 2021Quote: ... A primary antibody dilution was prepared in the blocking solution with the antibodies diluted 1:200 for HIF1α (BD Bioscience, 610958), 1:50 for phosphor-S6 (CST ...
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bioRxiv - Cell Biology 2021Quote: ... Crypts were mixed with Col6a1cre-GFP+ and GFP− IMCs sorted by FACS at passage 1 and subsequently resuspended in Matrigel (BD Biosciences) at 250 crypts/50.000 IMCs/30 μl in 48-well plates ...
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bioRxiv - Cancer Biology 2021Quote: ... Flow cytometry for negative checkpoint regulator was performed as previously described(14) using antibodies: BV510 mouse anti-human CD279 (PD1) (1:100; 563076, BD Horizon), PerCP-eFluor 710 mouse anti-human CD223 (LAG-3 ...
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bioRxiv - Cancer Biology 2021Quote: ... The levels of DNA-loaded MCM were detected by incubating cells in anti-MCM2 BM28 antibody (1:200, BD biosciences, 610700) for 1 hr at 37°C in the dark followed by incubation in anti-mouse-488 secondary antibody (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... 3 times 5 min washes with TNT were followed by a 1 h incubation with biotinylated goat anti-rat (559286, BD biosciences) 1:100 in TNB ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were activated in plates coated with anti-mouse CD3ε and anti-mouse CD28 antibodies (1 µg/ml, BD Biosciences) for up to 72h.
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bioRxiv - Immunology 2022Quote: ... into FACS buffer (1X PBS with 2% FBS and 1 mM EDTA) and analyzed with a FACS Aria flow cytometer (BD Biosciences).
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bioRxiv - Genomics 2022Quote: ... 1 μg of total RNA from NIH/3T3 cells was diluted with 500 μL of BD Rhapsody Lysis buffer (BD Biosciences) supplemented with 15 mM DTT (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... For measurements of CXCL13 the cells were cultured for 5 hours at 37°C in RPMI supplemented with 10% human AB serum and GolgiStop (1/1500; BD Biosciences). For cytokine measurements the cells were plated in the presence of anti-CD3/CD28 (Dynabeads® Human T-activator CD3/CD28 ...
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted by centrifugation at 600 x g for 6 minutes and fixed in 100 μL per 1×106 cells of PhosFlow Lyse/Fix buffer (BD Bioscience) at 37 °C for 10 minutes ...
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bioRxiv - Immunology 2022Quote: Lymphocytes were isolated from the lungs of VSV-OVA-challenged mice and stimulated ex vivo with OVA peptide 1 mM) in the presence of Golgi-Plug (BD Biosciences) for 5 hours ...
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bioRxiv - Immunology 2022Quote: ... and the remaining cells were incubated for 5-6 hrs at 37°C in the presence of 1 uM of the indicated peptide and brefeldin A (GolgiPlug; BD Pharmingen). Surface staining was done overnight at 4 degrees ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed in 1% (vol/vol) formaldehyde for 30 min at 4°C and analyzed on an LSRFortessa (BD Biosciences) flow cytometer ...
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bioRxiv - Developmental Biology 2022Quote: ... Data concerning bTg-GFP (endogenous expression) and Nkx2-1 expression were obtained and processed using a LSRFortessa X-20 flow cytometer and FACSDiva software (BD Biosciences), respectively ...
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bioRxiv - Microbiology 2022Quote: ... Live CD45+Ly6G+CD11b+Wasabi+ or Wasabi- cells were sorted by FACS in PBS supplemented with 1% BSA using a FACS ARIA Fusion (BD Biosciences). Single cell RNA-sequencing libraries were prepared according to the manufacturer’s instructions using Chromium Single Cell 3′ Library & Gel Bead Kit v3 (10x Genomics ...
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bioRxiv - Cell Biology 2022Quote: ... Cells expressing desired transgenes were isolated by culturing in growth media supplemented with puromycin (1 μg/mL) or using fluorescence-activated cell sorting (FACS) as appropriate (FACS Aria3, BD Biosciences).
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bioRxiv - Immunology 2020Quote: Tumor cells co-cultured with CAR T cells for 1 to 4 h were fixed in 4% PFA for 10 minutes at 4°C and then washed in PBS and stained with anti-ICAM-1 (REA 266, Miltenyi) or anti-phosphorylated STAT1 pY701 (clone 4a, BD Biosciences) antibodies ...
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bioRxiv - Immunology 2020Quote: ... Flow cytometry experiments were performed using PE-anti-human CD54 (ICAM-1) (REA266, Miltenyi) and APC-anti-human CD20 (clone L27, BD Biosciences). Antibodies for blocking LFA-1 - ICAM-1 interaction were anti-ICAM1 (clone HA58 ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked with Purified Rat Anti- Mouse CD16/CD32 Mouse BD Fc Block™ (2.4G2, BD Pharmingen, 1:1000). Cell surface antigens were stained in PBS containing 2 % (vol/ vol ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were suspended in 200 µl of 1% FBS and cells were sorted by GFP expression using FACSCVantage SE (BD Biosciences). The cell cycle profiles of GFP-positive and GFP-negative cells were determined by Hoescht flourescence using a FACSCalibur flow cytometer (Becton Dickinson) ...
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bioRxiv - Immunology 2021Quote: ... For each sample 200,000 cells were incubated with suitable concentrations of CD4 Nbs coupled to CF568 in combination with 1:500 dilution of anti-CD3-FITC (BD Biosciences) at 4°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... or GFP-Nb-Cy5.5 (∼51 nM) in 1% FPS/PBS at 4°C for 20 min and subsequently analyzed on a LSR-II cytometer (BD biosciences).
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bioRxiv - Microbiology 2021Quote: Nectin-1 was detected on the surface of cells by staining them with anti-nectin-1 antibody CK41 conjugated to PE (BD Biosciences). HVEM was detected on the surface of cells using the anti-HVEM polyclonal antibody ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated in PGB for 25 min with antibodies against Lewis-X (1:20, LeX/CD15 FITC, MMA; BD Bioscience), CD133-APC (1:50,13A4 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in 3ml FACS buffer before being fixed in 1% paraformaldehyde and analyzed within 5 days using a Fortessa Flow Cytometer and BD FACSDiva Software (BD Biosciences). Flow cytometric data were analyzed using FlowJo™ (Treestar ...
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bioRxiv - Immunology 2020Quote: ... 0.5-1×106 events were collected in each case on a BD LSR Fortessa X-50 flow cytometer (BD Immunocytometry Systems). Electronic compensation was performed with antibody capture beads (BD Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stimulated with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) plus 1 µg/ml ionomycin for 4 h at 37°C in the presence of GolgiPlug/GolgiStop (BD Pharmigen). Cells were stained for surface molecules as described above ...
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bioRxiv - Cancer Biology 2020Quote: ... single CD44+CD39+PD-1+CD8+ T cells as well as other CD44+CD8+ T cells were sorted using an ARIA III Sorter (BD Biosciences).