Labshake search
Citations for Becton, Dickinson and Company :
5501 - 5550 of 5734 citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2020Quote: ... 0.5 × 106 DAPI-/SSEA4+/TRA-1-60+ cells were collected for each cell line using the BD FACSAria™ II flow cytometer (BD Biosciences). Collected cells were pooled and plated at a density of 30 000 /cm2.
-
bioRxiv - Evolutionary Biology 2020Quote: ... reduced to a single-cell suspension and seeded into a 96-well plate at a density ranging between 1000 – 20 c/w in mTesRTM1 supplemented with 1 nM ROCK inhibitor (BD Biosciences, 562822). Alternatively ...
-
bioRxiv - Cell Biology 2020Quote: ... The following antiboodies were used in combination for the depletion of lineage-positive cells: anti-CD4 (RM4-5, BD Biosciences, 1:100), -CD8 (53-6.72 ...
-
bioRxiv - Neuroscience 2021Quote: ... Membranes were allowed to dry and then hydrated and blocked for 1h at RT with 5%milk/TBS-T and incubated overnight at 4ºC with the primary antibody mouse monoclonal anti-α-Syn 1:1000 (BD Biosciences, 610787) in 5%milk/TBS-T ...
-
bioRxiv - Developmental Biology 2021Quote: ... for 60 min, the sections were reacted with the primary antibody (for CD31, 1:100 rat monoclonal anti-CD31 (BD Biosciences, 550274); for Cytokeratin ...
-
bioRxiv - Cell Biology 2021Quote: Cells were treated with 25uM BrdU for 1 hour and BrdU staining detected using FITC Mouse Anti-BrdU Set (BD Biosciences, 556028). Cells were resuspended in 10μg/ml 7-Amino-Actinomycin D (7-AAD ...
-
bioRxiv - Cell Biology 2021Quote: ... The ZsGreen-ODC (ornithine decarboxylase) cells were previously generated by transfection of MelJuSo cells with the ZsProsensor-1 plasmid (BD Bioscience Clontech)20 ...
-
bioRxiv - Immunology 2021Quote: ... For cell staining cells were first blocked with 1 μg/ml Fc-Block (mouse Fc Block, BD Biosciences, San Jose, CA, USA) for 15 minutes at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... enriched tumor-infiltrating CD8+ T cells were re-stimulated with 1 μg/ml ionomycin (IM) and 25 ng/ml phorbol myristate acetate (PMA) in the presence of GolgiPlug (BD Biosciences, USA) for 4 hours at 37 °C ...
-
bioRxiv - Immunology 2020Quote: ... up to 5×106 leukocytes were incubated with 5 μg/mL E6 peptide or 1 μg/mL α-CD3e (clone 145-2C11, BD Biosciences) in the presence of 1:1000 Golgi-plug for 5 hours ...
-
bioRxiv - Immunology 2020Quote: ... and BV421 rat anti-mouse TNF-α BD Biosciences in a total of 100 μL of 1× Perm/Wash Buffer (BD Biosciences). After two washes ...
-
bioRxiv - Immunology 2020Quote: Cryopreserved lymphocytes from BAL were thawed and stimulated with H1N1pdm09 (MOI 1) or medium as a control for 18 hours at 37°C prior GolgiPlug (BD Biosciences, UK) addition as per manufacturer instructions ...
-
bioRxiv - Immunology 2020Quote: ... The cells were blocked with 4% BSA and stained with a 1:200 (v/v) dilution of Alexa Fluor 647 Mouse anti CD247 pY142 antibody (BD Bioscience #558489) for 1 hour at room temperature ...
-
IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... After washing with 1% BSA-PBS slides were incubated with secondary goat anti-rat and goat anti-rabbit biotinylated antibodies for 30 min at RT followed by 30 min of incubation with streptavidin–HRP (1:500; BD Pharmingen, 554066). For developing DAB substrate (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... before resuspending in 25 μl of 0.2µm-filtered 1% BSA/PBS containing monoclonal FITC-anti-mouse IgA (BD Pharmingen, 10 µg/ml) or Brilliant violet 421-anti-IgA (BD Pharmingen ...
-
bioRxiv - Immunology 2021Quote: ... onto a 40 µM nylon cell strainer and softly mashing the organ through the cell strainer into a petri dish using the plunger tip of a 1 ml syringe (BD Plastipak 303172). Cell suspensions were filtered several times and filters were rinsed with fresh buffer to obtain a homogeneous single-cell suspension ...
-
bioRxiv - Cell Biology 2022Quote: ... Pelleted cells were incubated with anti-GFP-AF647 antibody (1:150, Alexa Fluor 647 Mouse Anti-GFP Clone 1A12-6-18, BD Biosciences 565197) or rabbit anti-ITGA5 (1:100 ...
-
bioRxiv - Molecular Biology 2022Quote: ... using a 23 G needle and a 1 ml syringe.Blood was collected into a serum separator tube (365967, BD, Franklin Lakes NJ), allowed to clot at room temperature for at least 10 minutes ...
-
bioRxiv - Cell Biology 2022Quote: The following antibodies were obtained commercially and used at the indicated dilutions for immunofluorescence: mouse monoclonal TOM20 (BD Biosciences #612278, 1:1000), TOMM20 (Abcam #ab186734 ...
-
bioRxiv - Cell Biology 2022Quote: ... Rings were fixed after 7 days with 2% PFA and stained with rat PECAM (dilution 1:100, Rat monoclonal, clone MEC 13.3; BD Biosciences, Cat# 557355). Rings were imaged in 3D using the ZEISS Lightsheet Z.1 microscope ...
-
bioRxiv - Immunology 2022Quote: ... 1 μg of purified rat anti-mouse CD16/CD32 was added to each pool (Mouse BD Fc Block, BD Biosciences, cat. 55142) and incubated for 30 minutes at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... 1 μg of purified rat anti-mouse CD16/CD32 was added to each pool (Mouse BD Fc Block, BD Biosciences, cat. 55142) and incubated for 30 minutes at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... Solution was washed 3x with FACS buffer following same centrifugation as above and then resuspended in 500 uL of FACS buffer and 1 uL DAPI (BD Biosciences, 564907) and filtered into filter-top FACS tubes ...
-
bioRxiv - Immunology 2022Quote: ... Solution was washed 3X with FACS buffer following same centrifugation as above and then resuspended in 500 uL of FACS buffer and 1 uL DAPI (BD Biosciences, 564907) and filtered into filter-top FACS tubes ...
-
bioRxiv - Microbiology 2022Quote: ... gonorrhoeae testing contains 3.6% GC agar base (Oxoid™ GC agar # CM0367) and 1% defined growth supplement (BD BBL™ IsoVitaleX # 211876). Compound solutions described above were incorporated into GC agar medium to pour rectangular plates that represent 2-fold serial dilutions consisting of 12 compound dilution steps of test or control article ...
-
bioRxiv - Physiology 2022Quote: ... Venous peripheral blood (4-6 ml for adults, 1- 3 mL for children and infants) was collected on-site on EDTA (BD vacutainer©) and immediately stored at 4-8°C until use ...
-
bioRxiv - Neuroscience 2023Quote: ... for 10 min at 37 °C and 5 % CO2 before they were stained with Fixable Viability Stain 510 (1:100, #564406, BD Horizon, USA) for 15 min at room temperature (RT ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Cells were then washed with PBS+5% FBS and stained with 1:100-dilutions of AF647-conjugated anti-PAX-6 (BD Biosciences; 562249) and PerCP-Cy5.5-conjugated anti-SOX-2 (BD Biosciences ...
-
bioRxiv - Synthetic Biology 2023Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...
-
bioRxiv - Immunology 2024Quote: ... and staining of surface markers with a mixture of conjugated antibodies 1:250 in 2%FBS PBS 1mM EDTA buffer with 10% Brilliant Stain Buffer Plus (BD, Cat#566385) for 30 min on ice ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The samples were diluted by adding 400 μL of 1×Annexin V binding buffer and then analyzed using Fluorescence-Activated Cell Sorting (FACS) (BD Biosciences, USA). At least 10,000 cells were measured for each sample.
-
bioRxiv - Cell Biology 2024Quote: ... 10 menstrual endometrial fragments (diameter 1-2 mm) from donor animals were inserted using two 14G catheters (Insyte, Vialon, BD, Madrid, Spain) under laparoscopic guidance in both left and right flank and behind the uterus ...
-
bioRxiv - Cancer Biology 2024Quote: ... and stained with the following conjugated antibodies (1/100 dilution in PBS) for 15 minutes at 4°C: CD44 (PE, BD Biosciences 555479), EpCAM (FITC ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were counted using a hemacytometer and then treated with an Fc blocking buffer containing anti-Cd16/32 (BD Biosciences, 1:500) for 20 minutes at 4 degrees Celsius before a 30 minute incubation with antibody master mixes for the myeloid or lymphoid panels at 4 degrees Celsius ...
-
bioRxiv - Bioengineering 2024Quote: ... 3-4×105 B16F10-ZsGreen cells were implanted subcutaneously (s.c.) into the right flank of mice after shaving through a 25G x 1 ½ in needle (BD PrecisionGlide Needle #305127). For the growth control and survival study ...
-
bioRxiv - Cell Biology 2024Quote: ... and anti-CD28 antibodies (eBioscience, clone 37.51, 1 µg/ml) on TC plates pre-coated with anti-hamster antibody (BD Pharmingen, G94-56), and IL-2 (Immunotools ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
-
bioRxiv - Immunology 2024Quote: ... MASP-1/-3/MAP-1 mab 8B3 or F(ab’)2 fragments generated from an irrelevant mouse IgG1 mAb clone MOPC-31C (BD Biosciences, 557273) were coated on maxisorp plates at 4 °C ON and afterwards washed and blocked for 1 hr in TBS/T ...
-
bioRxiv - Immunology 2024Quote: ... Cells were washed with FACS staining buffer (PBS supplemented with 2% fetal bovine serum and 1 mM EDTA) and incubated with Fc receptor blocker purified rat anti-mouse anti-CD16/CD32 (BD Biosciences, 553142) for 20 min at 4°C ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were then immuno-stained 1 hour at room temperature with a panel of antibodies: anti-CD3 (563725, BD, Franklin Lakes, NJ), anti-TNFα (Biolegend 502938) ...
-
bioRxiv - Cancer Biology 2024Quote: CAR T-cells were incubated with target cells at an effector-to-target (E:T) ratio of 2:1 in complete RPMI 1640 medium containing GolgiStop™ Protein Transport Inhibitor Reagent (BD Bioscience, USA) and CD107a APC antibody (BD Biosciences ...
-
bioRxiv - Microbiology 2023Quote: ... after which cells were stained with SYTO 16 at a final concentration of 1 μM and counted via a BD FACS Verse flow cytometer (BD, Erembodegem, Belgium). Data was analyzed using FlowJo ...
-
bioRxiv - Immunology 2023Quote: ... cells were seeded in 24-well plates or CELLview Cell Culture Dish with four compartments) (USA Scientific, 5662-7870) in culture media with 1 μg/ml propidium iodide (PI, BD Bioscience, 556463) followed by calculating PI positivity on either an Inverted Nikon Ti2 fluorescence microscope with a 40X objective (N.A ...
-
bioRxiv - Cell Biology 2022Quote: ... labelled with cell hashing antibodies and isolated by FACS (all steps at 4°C): Cell suspensions were incubated with Fc-block (1:50, BD Pharmingen 553142) for 10min and subsequently incubated with mouse CD45 MicroBeads (Miltenyi Biotec ...
-
bioRxiv - Bioengineering 2023Quote: ... at 10 × 106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences, US) and CD24 (PE-Cy7-conjugated ...
-
bioRxiv - Microbiology 2023Quote: ... at 37°C and 200 rpm shaking for 12-18 hours and subsequently diluted 1:200 fold in BBL Mueller-Hinton II broth cation-adjusted (MHII, BD cat. #212322) (+ 100 μg/mL Amp for pET17b plasmid carrying strains ...
-
bioRxiv - Cell Biology 2023Quote: ... VE-cadherin was stained 1:200 with the live labeling antibody Alexa Fluor® 647 Mouse Anti-Human CD144 (BD Pharmingen™), 2 min prior to imaging ...
-
bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H: BD construct #8) and each column is a single AD construct (For example column 1 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were gated on FSC and SSC followed by single cell gate on FSC-HH/FSC and SSC-H/SSC and a LIVE/DEAD near IR marker (1:1000, BD Biosciences, L34976). The following antibodies were used ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were processed as for CDC45 but without the methanol fixation step and with a 1:1000 dilution of mouse monoclonal anti-MCM2 (BD Biosciences, 610701) for 14 hours at 4°C ...