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Citations for Becton, Dickinson and Company :
5601 - 5650 of 5734 citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and late stages of fermentation were serially-diluted and plated onto YEPD media (1% (w/w) yeast extract (BD BactoTM, Sparks, MD, USA), 1% (w/w ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated in the FC wash buffer (2h at 4 °C) containing FITC-coupled anti-CD44 antibody (1 to 80 BD Pharmingen Cat.No. 555478) or its isotype control (FITC Mouse IgG2b ...
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bioRxiv - Neuroscience 2020Quote: Brains from paraformaldehyde-perfused 12 month-old mice were sectioned and 30 µm free-floating sections were incubated overnight at 4°C with primary antibodies (anti-αsyn, BD Transduction, 1:700), anti-DNAJC5 (Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... anti-O4 (130-118-978) (Milteny Biotec, Bergisch, Germany), anti-O1 (FAB1327V) (Novous Biologicals, Littleton, CO) and anti-CD45 (OX-1 (ROU)) (BD, Franklin Lakes, NJ). Dead cells were excluded using near IR Live/Dead (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... harvested bone marrow cells were labeled using a two-step sequential antibody labeling procedure using the following primary conjugated antibodies: 1:200 dilution of Anti-Mouse CD5 FITC antibody (BD Biosciences, Cat#: 553020), 1:100 dilution of Anti-Mouse B220 FITC antibody (BD Biosciences ...
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bioRxiv - Genomics 2022Quote: ... Cells were labeled with the following antibodies: 1:400 dilution of Anti-mouse CD45 antibody conjugated to APC-Cy7 (BD Biosciences, Cat#: 557659), 1:200 dilution of Anti-mouse TER-119 antibody conjugated to APC-Cy7 (BD Biosciences ...
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bioRxiv - Physiology 2020Quote: ... Sections were blocked in TBS containing donkey serum or PBS/0.1% Triton X-100 and stained for Vimentin (BD pharmingen #550563, 1:2000) and phospo-Akt (CST #4060 1:50 ...
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bioRxiv - Genetics 2020Quote: ... in 100 μl of a 1:200 cocktail of fluorescence-conjugated antibodies to 15 cell surface markers encompassing the major immune lineages B220 (BD, clone RA3-6B2), CD19 (BD ...
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bioRxiv - Immunology 2020Quote: ... Cells were then spun down at 500 × g for 5 min at 4°C and resuspended with the following antibody-conjugated fluorophores for 20 minutes at room temperature: anti-CD3-BV421 (BD, 1F4, 1:100), anti-CD4-PE-Cy7 (BD ...
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bioRxiv - Immunology 2020Quote: ... The cells were then washed with 1%FBS PBS and stained for 20 minutes at room temperature with the following panel of fluorescently conjugated antibodies diluted in 1%FBS PBS: CD56-PECy7 (BD Biosciences, clone NCAM16.2), CD16-PacBlue (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... Infected cells were selected with 1 μg/ml puromycin and isolated by FACS using a FACS-Aria II cell sorter (Becton Dickinson, BD, Franklin Lakes, USA) based on TurboRFP expression after the induction with 1 µg/ml of Doxycycline
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bioRxiv - Immunology 2020Quote: ... The cells were subsequently washed with FACS buffer (1% BSA and 0.1% sodium azide in phosphate-buffered saline) and stained with anti-HLA-A2 specific monoclonal antibody (clone BB7.2) (BD-Pharmingen, San Diego, CA) at 4°C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... washed three times with PBS 1% FBS and permeabilized with 1x Perm/Wash solution (diluted from BD Perm/Wash Buffer 5x in PBS) at RT for 15 min ...
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bioRxiv - Cell Biology 2020Quote: ... cerevisiae cells were grown at 25°C in YPD liquid medium containing 1% (w/v) Bacto yeast extract (BD Biosciences, San Jose, CA), 2% (w/v ...
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bioRxiv - Immunology 2021Quote: ... cells were stimulated with PMA (50 ng/mL) and Ionomycin (1 µg/mL) in presence of GolgiPlug™ and GolgiStop™ (BD biosciences) for 5 hours ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Cells were incubated in the dark with 2 μl/1×106 cells of CD16/32 antibody (Fc block; BD Biosciences, Mississauga, ON, Canada) for 15 minutes at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibody incubated for one hour at RT in 5% milk TBS-T at 1:1,000 (HRP-Anti-Mouse, BD Pharmigen, Goat Polycloncal, 554002). Between steps 3 × 5 minute washes were performed in TBST ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DriSolv methanol anhydrous was from Mettler Toledo (Columbus, OH) and TB syringes with 21G x 1 needles were from BD (Franklin Lakes, NJ). Aluminum pouches were from IMPAK (Los Angeles ...
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bioRxiv - Immunology 2021Quote: NK cells and cancer cells were cocultured at 10:1 ratio and supplemented with 20ul of PE mouse anti-human CD107a antibody (BD Pharmingen™, #555801) in a total volume of 220ul in a 96 well plate at 37C incubator for 90 minutes ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 45 min with the indicated antibodies: AlexaFluor® 647 anti-human CD107a antibody (diluted at 1/100, clone H4A3; BD Pharmingen TM), anti-human Perforin (10µg/ml ...
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bioRxiv - Immunology 2020Quote: ... and subsequently PBMCs were stimulated in R10 media with specific peptide pools or medium alone (DMSO control) in the presence of 1 μg/ml of α-CD28 (BD bioscience 555725) and α-CD49d (BD bioscience 555501 ...
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bioRxiv - Cell Biology 2020Quote: ... and rat α7 integrin-APC (1/1000; AbLab, Vancouver, Canada) and sorted using a FACS Aria II (BD Biosciences, San Jose, CA, USA). Satellite cells were isolated as CD31− ...
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bioRxiv - Biochemistry 2022Quote: ... a 1:100 dilution of primary polyclonal anti-GPR112 antibody was applied together with primary monoclonal anti-ß-catenin antibody (1:100, #610154, BD Biosciences, Europe) on antigen-retrieved and background reduced sections overnight at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Human platelets in the obtained blood were identified using APC-labeled polyclonal rabbit anti-human (h) CD41 and/or CD42b (BD Pharmingen, 1:100) and fluorescein isothiocyanate (FITC)-labeled polyclonal rabbit anti-mouse (m ...
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bioRxiv - Immunology 2022Quote: ... Cultures were deemed established when the cells stained positive for a melanoma tumor marker (MCSP-1 Miltenyi, cat# 130-117-347) and negative for a fibroblast marker (CD90, BD Pharmingen, cat# 561558). Tissues were harvested primarily by surgical resection ...
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bioRxiv - Immunology 2022Quote: ... cells were stained in FACS Buffer (1:200, PBS, 2% FBS, 2 mM EDTA, 0,05% NaN3) with antibodies recognizing: CD14 PECy7 (555399, BD Biosciences, Franklin Lakes, USA), CD16 BV510 (563830 ...
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bioRxiv - Microbiology 2022Quote: ... containing 5 mM CaCl2 and a 1:100 dilution of overnight host culture was overlaid atop a solid layer of Tryptic Soy Agar (TSA) (BD Diagnostics, NJ, USA) plates supplemented with 5 mM CaCl2 ...
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bioRxiv - Microbiology 2022Quote: ... was used as primary antibody for samples and mouse anti-clathrin heavy chain clone 23 (1:2,000; BD Biosciences, Franklin Lakes, NJ, USA) for the detection of clathrin heavy chain as a loading control ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were dispersed into a single cell suspension using 0.05 % trypsin and subsequently incubated with an anti CD144 antibody (anti-VE-Cadherin; BD Biosciences, #562242, 1/50) for 30 min on ice ...
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bioRxiv - Genomics 2024Quote: ... 2.5 x 105 cells were stained for 75 minutes at room temperature with PE Mouse anti-PDX1 Clone-658A5 (BD Biosciences; 1:10) and Alexa Fluor® 647 Mouse anti-NKX6.1 Clone R11-560 (BD Bioscience ...
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Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: ... cells were restimulated ex vivo for 4-5h at 37 °C in complete RPMI medium (RPMI supplemented with 2mM L-Glutamine and 10% fetal calf serum (FCS)) with GolgiPlug (BD, 1:1,000 dilution), phorbol 12-myristate 13-acetate (50ng/ml ...
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bioRxiv - Immunology 2024Quote: ... Purified CD4+ cells were cultured in 24-well round-bottom plates (1×106 cells/well) with plate-bound anti-CD3e (2.5 μg/ml, clone 2C11, BD Biosciences, San Diego, CA), soluble anti-CD28 (2 µg/ml ...
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bioRxiv - Physiology 2023Quote: ... p = 0.2 (sum of shared targets between 1/0 and 5/0 R-βHB ex vivo groups and BH-BD targets, divided by n), and q = 0.8 ...
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bioRxiv - Biochemistry 2023Quote: Plasma was obtained by withdrawing blood from eight healthy donors (specified in Supplementary Table 1) in EDTA tubes (BD Vacutainer K2E; REF 367525). The tubes were inverted three times and centrifuged at 2000xg for 20 min at 4°C ...
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bioRxiv - Pathology 2023Quote: ... A final resuspension of splenocyte pellet with 100 µl of PBS-FBS 1% was performed before transfer to Micronic tubes (Cat# 2517810, Dutscher, France) for flow cytometry analysis (LSRFortessa™, BD Biosciences, USA). Data was analyzed using FlowJo software (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Microbiology 2023Quote: ... and allowed to gel over a 1-mL layer of acellular collagen in six-well culture inserts with 3-μm-pore polycarbonate filters (BD Biosciences NJ, USA). Gels were allowed to contract for 4–5 days before seeding with 3×105 HaCaT keratinocytes in serum and mitogen-supplemented DMEM/F12 raft medium ...
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bioRxiv - Developmental Biology 2023Quote: ... FGF10 expression was induced by the addition of isopropyl-1-thio-B-D-galactopyranoside to RosettaTM2(DE3)pLysS carrying pET21d-FGF10 in 2x YT medium (BD Biosciences, Cat#244020) with Carbencillin (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... cells were washed in perm buffer (PBS, 0.5% BSA, 0.1% Saponin) and stained for IFN-γ (APC rat anti- mouse IFN-γ; 1:100; BD Biosciences, Cat. 554413) and TNF (PE rat anti-mouse TNF alpha ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then suspended in 0.5% Tween 20 (vol/vol) plus 1% BSA/PBS and incubated for 30 minutes at room temperature with anti-BrdU FITC (BD Biosciences, Cat. #347583) at a concentration of 0.5 µg/106 cells ...
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bioRxiv - Cancer Biology 2023Quote: ... 200 μL per 1x10e6 cells) with human IgG-Fc block (1:100) followed by the incubation with streptavidin-magnetic bead particles (BD Biosciences, Ref#557812) at 50 μL per 1x10e6 cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with flow cytometry antibodies (Supplemental Table 9) in flow cytometry staining buffer (PBS, 1% BSA) and brilliant staining buffer (563794, BD, Franklin Lakes, NJ). Cells were analyzed using a 5-laser (16UV-16V-14B-10YG-8R ...
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bioRxiv - Pathology 2023Quote: ... The culture medium was sampled by adding 1-3 drops to microscope slides followed by Gram stain (BD BBLTM Gram Stain Kits; B12539) and imaging.
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bioRxiv - Immunology 2023Quote: ... pre-coated for 1 hour at 37°C with anti-CD3 antibodies (5ug/mL, clone: 145-2C11, BD Biosciences, San Diego, CA, USA) and supplemented with soluble anti-CD28 antibodies (2ug/mL clone ...
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bioRxiv - Cell Biology 2023Quote: ABT-737-treated or SARS-CoV-2-infected cells (detached using Trypsin/EGTA) and their respective conditioned media were combined and stained with fluorescently-labelled annexin A5 (BD Biosciences, 1:2,000) and 0.2 μM TO-PRO-3 (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... aeruginosa PAO1 was diluted 100-fold into 300 mL of M9 medium (1% w/v 5X M9 Salts (BD Difco, Franklin Lakes, NJ), 1.5% w/v Bacto Casamino Acids with low iron and salt content (BD Difco) ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenized tissue was washed with 3-4 ml washing buffer which was fresh PBSB 1% and then was filtered through a 70-µm cell strainer (BD Bioscience, NY, USA). Then using a long tube ...
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bioRxiv - Physiology 2023Quote: ... The platelets were then fixed with 1% formaldehyde and the fluorescence were determined using BD LSRFortessaTM cell analyzer (BD Biosciences, Mississauga, ON, Canada). A negative control with no antibodies was included.
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked with 5% skim-milk diluted in 1X TBST and incubated with primary antibodies overnight [RB (BD Pharmingen #554136, 1:500), p53 (Pierce #MA5-12453 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed at least three times with 1×PBS (pH 7.4) before flow cytometric analysis using BD Accuri C6 (BD Biosciences, San Jose, USA) with FITC channel.