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Citations for Becton, Dickinson and Company :
5301 - 5350 of 5708 citations for Herpes Simplex Virus 1 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed twice with 1× PBS and flow cytometry analysis was performed on a FACS Canto II flow cytometer (BD Biosciences); data analysis was carried out using FlowJo software (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... were re-stimulated in vitro for 5h at 37°C 5% CO2 in cRPMI supplemented with GolgiStop (1/250; BD bioscience), GolgiPlug (1/500 ...
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bioRxiv - Immunology 2022Quote: ... cultured splenocytes were resuspended in 40 μL FACS buffer containing fluorochrome-conjugated antibodies and incubated 1 hr at 4 °C followed by cell fixation using Cytofix/Cytoperm buffer (BD Bioscience) for 20 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... was added and 0.6-1×106 lymphocyte-gated events were immediately acquired on an LSRII SORP (BD, San Diego, CA, USA).
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bioRxiv - Immunology 2022Quote: ... ELISAs were performed using paired rat anti-mouse IgE antibodies (capture with 1 μg/mL purified R35-72, BD Biosciences, detect with biotinylated R35-118, BD Biosciences) and streptavidin-HRP (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... and others were fixed using PBS containing 1% paraformaldehyde (Labsynth, Diadema, SP, Brazil) and acquired in FACS Canto II (BD Biosciences). Analyses were performed in FlowJo software (Becton Dickinson and Company).
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bioRxiv - Microbiology 2022Quote: ... xanthus cells were grown in liquid CYE medium (10 mM MOPS pH 7.6, 1% (w/v) Bacto™ casitone (BD Biosciences), 0.5% yeast extract and 8 mM MgSO4 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antibody bound to RBD in the presence of competitor was stained with Streptavidin-PE at a 1:1000 dilution (BD Biosciences) and analysed on a 200 Bioplex instruments ...
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bioRxiv - Microbiology 2022Quote: ... 50 μL of DEs were diluted into 500 μL of FACS diluent buffer (PBS + 1% Tween-20) in a 5 mL 12 × 75 mm round bottom FACS tube (BD Biosciences). After standard autocalibration on the Sony SH800 ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting pellet was resuspended in 1 ml of complete neurosphere medium containing epidermal growth factor (EGF, 20 ng/ml; receptor grade, BD Biosciences) and basic fibroblast growth factor (bFGF ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD8 APC (1:100, catalog no. 553035; BD Biosciences); rat anti-CD45 PE (1:100 ...
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bioRxiv - Immunology 2022Quote: ... cells were stained for p-STAT1 (clone 4alpha) or p-STAT3 (clone 4P/STAT3) for 1 hour (all from BD Biosciences) and were acquired on FACS LSRFortessa flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... Data acquisition and analysis: 0.5-1×106 events were collected on a BD LSR Fortessa X-50 flow cytometer (BD Immunocytometry Systems). Electronic compensation was performed with antibody capture beads (BD Biosciences) ...
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bioRxiv - Physiology 2022Quote: ... Novus) and F(ab’)2-donkey anti-Rabbit IgG (H+L) secondary antibody PE (1:100, 12-4739-81, BD bioscience) at 4℃ for 1h ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were submitted to the same procedure as for flow cytometry analysis but without 1% PFA treatment and processed using BD FACSAria (BD Biosciences).
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bioRxiv - Molecular Biology 2022Quote: ... Confluent definitive endoderm cells are then passed 1:3 in presence of Rock Inhibitor on growth factors reduced Matrigel (BD Biosciences) and cultured for 8 days in differentiation medium (DMEM F12 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were engrafted in NSG mice by subcutaneous injection of 1 × 106 cells of H2081 in RPMI1640 medium supplemented with 50% Matrigel (BD Biosciences). Ten days after the injection ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were stained with anti-HbF APC antibody (dilution 1:50) and was acquired and analysed using Cytoflex LX Flow Cytometer (Beckmann Coulter) or AriaIII flow cytometer (BD Biosciences) and analysed using FlowJo (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... Then live Tetr-gag+Pent-gag+CD3+CD8+ cells were sorted by flow cytometry into PBS buffer 1% BSA 2 mM EDTA using a FACSAria III (BD Biosciences) equipped with 488 ...
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bioRxiv - Developmental Biology 2022Quote: ... Primordial germ cells were purified based on the expression of Zombie Red−/SSEA-1+/CD61+ using BD FACS Aria II Cell Sorter (BD Bioscience). Somatic gonad cells were purified based on the expression of Zombie Red−/SSEA-1−/CD61 ...
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bioRxiv - Microbiology 2022Quote: ... or the TAPI (prepared as described above) was administered directly into the pocket via a 1 mL syringe (BD, cat# 309628). The implants ...
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bioRxiv - Cell Biology 2022Quote: ... 1 % bovine serum albumin (BSA, Jackson, cat. no. 001-000-162) and 10% Perm/Wash buffer (BD Biosciences, cat. no. 554714) at RT for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... with phorbol 12-myristate 13-acetate (PMA, 20 ng/ml) plus ionomycin (1 mg/ml) in the presence of GolgiPlug/GolgiStop (BD Biosciences). Fluorescence was quantified using an LSR-II or Fortessa X20 flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... Cell purity was determined by flow cytometry (see Figure S6A) using antibodies specific against CD20-FITC (1:100 345792, BD Biosciences), CD14-PerCP (1:50 ...
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bioRxiv - Microbiology 2024Quote: ... Sputum samples were diluted in 1% proteose peptone and then plated on oxidation/fermentation-polymyxin-bacitracin-lactose (OFPBL) media (BD Biosciences) to select single B ...
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bioRxiv - Genomics 2024Quote: ... cells were resuspended in 110 µl of FACS Buffer supplemented with 1 µM Propidium Iodide and analyzed on a FACS Calibur Flow Cytometer (BD Biosciences).
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then spun down and resuspended in 100LμL of buffer containing fluorescently conjugated antibodies for one hour (5LμL of each antibody per sample): APC-Cy7 Mouse Anti- Human CD4 (1:20 dilution; BD Biosciences, #557871), APC-Cy7 Mouse IgG1 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were stained with rabbit anti-GRA7 (1:5000) (gift from John Boothroyd, Stanford) and rat anti-LAMP1 (1D4B, BD Biosciences) (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... and then incubated overnight with hamster anti-CD3ε antibody diluted in 1% goat serum/0.3% Triton-X100/TBS (BD Biosciences, 550277). The next day samples were washed in TBS and incubated at room temperature in goat anti-hamster 647 (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were pelleted and washed twice with staining buffer and suspend 1 ml of staining buffer with DAPI for an end concentration of 1 x 106 cells/ml and DAPI (0.2 ug/ml; BD Biosciences, 564907) added ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.2) or BS-N buffer supplemented with 1% casein hydrolysate (casamino acids technical grade; Becton, Dickinson and Company, NJ, USA) at an OD660 of 4.0 was transferred to a ProteoSave SS 1.5-mL centrifuge tube (Sumitomo Bakelite ...
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bioRxiv - Plant Biology 2023Quote: ... The flowers and the fixing solution were removed and replaced with 1 µg/mL DAPI (4′,6-diamidino-2-phenylindole, BD Biosciences) diluted in pollen isolation buffer before visualizing under a fluorescence microscope using a 420 nm filter (Zeiss LSM880 Inverted Confocal Microscope) ...
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bioRxiv - Cell Biology 2024Quote: ... and screened for protein levels via Western blots with monoclonal mouse anti-IQGAP1 (1:1000; 610612, BD Biosciences, East Rutherford, NJ), polyclonal rabbit α-tubulin (1:2500 ...
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bioRxiv - Developmental Biology 2024Quote: ... DAPI was added to mark the dead cells (1/2000) and the cells were sorted using the FACS Aria I cytometer (BD Biosciences). Twenty thousand Epcam-positive and Tomato-negative (PlusMinus ...
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bioRxiv - Developmental Biology 2024Quote: All cell culture of all species was maintained at 37°C with 5% CO2 and 5% O2 on Matrigel (1:100, BD Corning) coated plates unless otherwise specified ...
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bioRxiv - Immunology 2023Quote: ... cells were counted then stained with optimal concentrations of the following fluorophore-antibody conjugations: BB700 anti-IgG1 (clone A85-1, BD Biosciences), BB700 anti-IgG2a (clone R2-40 ...
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bioRxiv - Microbiology 2024Quote: ... xanthus cells were grown in liquid CYE medium (10 mM MOPS pH 7.6, 1% (w/v) Bacto™ casitone (BD Biosciences), 0.5% yeast extract and 8 mM MgSO4 ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed once with FACS buffer and fixed with 1% paraformaldehyde for 30 minutes prior to analysis on an LSRII flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... diluted 1:10 in DI H2O for 10 minutes within 1 hour of sample collection and then washed twice with Cell Staining Buffer (BD Biosciences). For cell surface staining ...
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bioRxiv - Cell Biology 2024Quote: ... organoids were dissociated and mCherry positive cells (marking successful electroporation of the pCAS9-mCherry-Frame+1 plasmid) were sorted on ARIAIII sorter (BD Biosciences) and seeded in Matrigel with normal organoid media supplemented with rock inhibitor Y-27632 (10 μM) ...
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bioRxiv - Cancer Biology 2023Quote: ... per well of U-bottom plate and coincubated for 4h at 37°C in the presence of NH4Cl and 1 µl Golgi PLUG (BD Biosciences). After that time cells were washed three times with 200 µl PBS and centrifuged (5 min ...
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bioRxiv - Immunology 2022Quote: ... The permeabilized cells were stained with intracellular cytokine staining antibodies for 20 min at room temperature in 1× perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before acquisition using the BD Symphony Flow Cytometer and the associated BD FACS Diva software ...
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bioRxiv - Immunology 2023Quote: ... Functionalised agarose beads were washed twice and then incubated with 20 nM of the GFP-streptavidin tetramers and anti-mouse IgG2a/b BV421 antibody (1:10 dilution, clone R2-40, BD Bioscience) for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted by their distinct proliferation peaks (peak 0, peak 1 and peak 2) on FACS-Aria and Influx Cell Sorters (BD Biosciences). Cells were kept on ice and after washing with cold PBS were immediately resuspended in Trizol for RNA extraction ...
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bioRxiv - Cancer Biology 2023Quote: ... tumor or normal organ tissue slides were incubated with primary antibody of Ki-67 (1:10000 dilution; Cat# 550609, BD Biosciences), p-Abi-1-S323 (1:500 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Gates were determined using the isotype controls mouse anti-IgG2b κ (APC-conjugated, Clone 27-35, 1:5, BD Biosciences, US) and mouse anti-IgG2a κ (PE-Cy7-conjugated ...
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bioRxiv - Developmental Biology 2023Quote: ... and stained with conjugated antibodies for 1 hour on ice: mouse anti-C-peptide antibody conjugated with Alexa Fluor 647 (BD Biosciences) and mouse anti-glucagon antibody conjugated with Bv421 (BD Biosciences) ...
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bioRxiv - Biophysics 2022Quote: ... samples were first resuspended in 15.5 μL PBS/0.1% adBSA and incubated with 0.5 μg of rat anti-mouse CD9-PE (Clone: KMC8, IgG2a, κ lot 7268877, BD Biosciences) or matched isotype antibodies (Rat IgG2a ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed and resuspended in 1% BSA with DAPI and detected by flow cytometry on a BD LSRFORTESSA X-20 (BD Biosciences). Data analysis was done using FlowJo V10.
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bioRxiv - Neuroscience 2023Quote: ... all samples were filtered through a 70-μm nylon mesh and blocked with anti-Fc CD16/32 (1:40; BD Biosciences). For FOXP3 staining ...