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Citations for Becton, Dickinson and Company :
5201 - 5250 of 5708 citations for Herpes Simplex Virus 1 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... For measurements of CXCL13 the cells were cultured for 5 hours at 37°C in RPMI supplemented with 10% human AB serum and GolgiStop (1/1500; BD Biosciences). For cytokine measurements the cells were plated in the presence of anti-CD3/CD28 (Dynabeads® Human T-activator CD3/CD28 ...
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted by centrifugation at 600 x g for 6 minutes and fixed in 100 μL per 1×106 cells of PhosFlow Lyse/Fix buffer (BD Bioscience) at 37 °C for 10 minutes ...
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bioRxiv - Immunology 2022Quote: Lymphocytes were isolated from the lungs of VSV-OVA-challenged mice and stimulated ex vivo with OVA peptide 1 mM) in the presence of Golgi-Plug (BD Biosciences) for 5 hours ...
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bioRxiv - Immunology 2022Quote: ... and the remaining cells were incubated for 5-6 hrs at 37°C in the presence of 1 uM of the indicated peptide and brefeldin A (GolgiPlug; BD Pharmingen). Surface staining was done overnight at 4 degrees ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed in 1% (vol/vol) formaldehyde for 30 min at 4°C and analyzed on an LSRFortessa (BD Biosciences) flow cytometer ...
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bioRxiv - Developmental Biology 2022Quote: ... Data concerning bTg-GFP (endogenous expression) and Nkx2-1 expression were obtained and processed using a LSRFortessa X-20 flow cytometer and FACSDiva software (BD Biosciences), respectively ...
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bioRxiv - Microbiology 2022Quote: ... Live CD45+Ly6G+CD11b+Wasabi+ or Wasabi- cells were sorted by FACS in PBS supplemented with 1% BSA using a FACS ARIA Fusion (BD Biosciences). Single cell RNA-sequencing libraries were prepared according to the manufacturer’s instructions using Chromium Single Cell 3′ Library & Gel Bead Kit v3 (10x Genomics ...
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bioRxiv - Cell Biology 2022Quote: ... Cells expressing desired transgenes were isolated by culturing in growth media supplemented with puromycin (1 μg/mL) or using fluorescence-activated cell sorting (FACS) as appropriate (FACS Aria3, BD Biosciences).
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bioRxiv - Immunology 2020Quote: Tumor cells co-cultured with CAR T cells for 1 to 4 h were fixed in 4% PFA for 10 minutes at 4°C and then washed in PBS and stained with anti-ICAM-1 (REA 266, Miltenyi) or anti-phosphorylated STAT1 pY701 (clone 4a, BD Biosciences) antibodies ...
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bioRxiv - Immunology 2020Quote: ... Flow cytometry experiments were performed using PE-anti-human CD54 (ICAM-1) (REA266, Miltenyi) and APC-anti-human CD20 (clone L27, BD Biosciences). Antibodies for blocking LFA-1 - ICAM-1 interaction were anti-ICAM1 (clone HA58 ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked with Purified Rat Anti- Mouse CD16/CD32 Mouse BD Fc Block™ (2.4G2, BD Pharmingen, 1:1000). Cell surface antigens were stained in PBS containing 2 % (vol/ vol ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were suspended in 200 µl of 1% FBS and cells were sorted by GFP expression using FACSCVantage SE (BD Biosciences). The cell cycle profiles of GFP-positive and GFP-negative cells were determined by Hoescht flourescence using a FACSCalibur flow cytometer (Becton Dickinson) ...
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bioRxiv - Immunology 2021Quote: ... For each sample 200,000 cells were incubated with suitable concentrations of CD4 Nbs coupled to CF568 in combination with 1:500 dilution of anti-CD3-FITC (BD Biosciences) at 4°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... or GFP-Nb-Cy5.5 (∼51 nM) in 1% FPS/PBS at 4°C for 20 min and subsequently analyzed on a LSR-II cytometer (BD biosciences).
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bioRxiv - Microbiology 2021Quote: Nectin-1 was detected on the surface of cells by staining them with anti-nectin-1 antibody CK41 conjugated to PE (BD Biosciences). HVEM was detected on the surface of cells using the anti-HVEM polyclonal antibody ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated in PGB for 25 min with antibodies against Lewis-X (1:20, LeX/CD15 FITC, MMA; BD Bioscience), CD133-APC (1:50,13A4 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in 3ml FACS buffer before being fixed in 1% paraformaldehyde and analyzed within 5 days using a Fortessa Flow Cytometer and BD FACSDiva Software (BD Biosciences). Flow cytometric data were analyzed using FlowJo™ (Treestar ...
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bioRxiv - Immunology 2020Quote: ... 0.5-1×106 events were collected in each case on a BD LSR Fortessa X-50 flow cytometer (BD Immunocytometry Systems). Electronic compensation was performed with antibody capture beads (BD Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stimulated with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) plus 1 µg/ml ionomycin for 4 h at 37°C in the presence of GolgiPlug/GolgiStop (BD Pharmigen). Cells were stained for surface molecules as described above ...
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bioRxiv - Cancer Biology 2020Quote: ... single CD44+CD39+PD-1+CD8+ T cells as well as other CD44+CD8+ T cells were sorted using an ARIA III Sorter (BD Biosciences).
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bioRxiv - Neuroscience 2021Quote: ... P0-P1 mouse pups were anesthetized in ice and then eyes were pierced at the edge of corneal by 30G × 1/2 needle (BD, 305106) under stereomicroscope ...
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bioRxiv - Immunology 2020Quote: ... followed by staining for 3 days at 37 °C with 1:100 dilutions of BV421-labeled mouse anti-human CD35 clone E11 (BD Biosciences) and Alexa Fluor 488-labeled mouse anti-Ki67 clone B56 (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... were plated on the lower wells and MCF7 or HFF (1 × 104 cells) were seeded into the top chamber (24-well insert; BD Bioscience) and then co-cultured for 24 hrs (Fig ...
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bioRxiv - Immunology 2021Quote: ... reactivation of latent HIV-1 was determined by quantifying the percentage of GFP+ cells using the FACS ARIA and analyzed using FlowJo (BD Biosciences) software ...
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bioRxiv - Developmental Biology 2022Quote: ... The single cells from BMP4 withdrawal plus FGF2 stimulated VFG culture were incubated only with 1:100 CD117-APC (BD Biosciences) in a similar condition to that described above ...
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bioRxiv - Immunology 2022Quote: ... The yeast clones were washed three times with cold PBS+1%FBS before analyzed by FACS using Aria II (BD Biosciences). Positive yeast clones were sorted and used for nanobody cloning.
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bioRxiv - Immunology 2022Quote: ... The permeabilized cells were stained with intracellular cytokine staining antibodies for 20 min at room temperature in 1 perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before acquisition using the BD Symphony Flow Cytometer and the associated BD FACS Diva software ...
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bioRxiv - Immunology 2022Quote: ... Cells were resuspended in 1% para-formaldehyde until acquisition on a BD Biosciences Symphony cytometer and analyzed using FlowJo (BD Biosciences). Caspase 3 staining was performed using PE Active caspase 3 Apoptosis Kit (BD ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunohistochemistry (IHC) was performed on FFEP blocks using mouse monoclonal antibodies SMARCB1/INI1 (Clone 25/BAF47, dilution 1:200, BD Bioscience) and CD70 (Clone 301731 ...
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bioRxiv - Genomics 2022Quote: ... This was followed by the secondary labeling step with 1:200 dilution of Streptavidin PE-Cy7 antibody (BD Biosciences, Cat#: 557598). Cells were isolated and sorted using an Influx Cell Sorter Instrument (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Tracheas were stretched out by pinning to SylGard-coated well of 6 well plate and fixed for 1 hr on ice in Cytofix/Cytoperm buffer (BD Biosciences). Immunofluorescent staining was performed in PBS with 0.25% Triton X-100 at 4°C as follows ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Tissues were incubated at 4°C overnight with the following primary antibodies: monoclonal mouse anti-carboxyl-terminal binding protein 2 (CtBP2) IgG1 at 1:200 (612044; BD Biosciences) counterstained with goat anti-Mouse IgG1 conjugated with Alexa Fluor 568 (#A-21124) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with 500 μL permeabilization buffer (from kit described above) and stained with 150 μL permeabilization buffer containing 1:400 Ki67-Alexa-Fluor antibody (561126, BD Biosciences) for 30 minutes at 4 °C ...
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bioRxiv - Developmental Biology 2019Quote: ... the cells were resuspended with blocking buffer (5% BSA in DPBS) and stained with PE-Cy7 rat anti-CD31 (1:400, 561410, BD biosciences), APC mouse anti-EpCAM (1:400 ...
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bioRxiv - Immunology 2019Quote: ... LN or spleen cells were stimulated with 50 ng/ml PMA and 1 μM ionomycin in the presence of Golgi-Stop (BD Biosciences) for 5 h ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was aspirated and the cell pellet was resuspended in 200uL of FACS buffer (PBS, 0.5% BSA, 2mM EDTA, 20mM Glucose) with 1:50 PE Rat Anti-Human CD49f antibody (BD Biosciences; 555736) and incubated on ice for 20 minutes ...
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bioRxiv - Immunology 2019Quote: ... harvested CD4+ T cells or lung single cells were stimulated with PMA (50 ng/ml) and ionomycin (1 µM) in the presence of GolgiStop (BD Pharmingen) for 6hr before FACS analysis.
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bioRxiv - Microbiology 2019Quote: ... Intrahaemocoelic injection of the larvae (n=10 per condition) was performed by a 1 mL tuberculin syringe (Norm-Ject) fitted with a custom 30½-gauge needle (BD PrecisionGlide) at a volume of 20 µL (∼20,000 cells ...
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bioRxiv - Cell Biology 2019Quote: ... VCAM-1 expression and E-selectin expression were detected by using APC-conjugated monoclonal antibodies anti-CD106 (VCAM-1, clone 51-10C9) and anti-CD62E (E-selectin, clone 68-5H11, BD Biosciences) respectively ...
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bioRxiv - Microbiology 2019Quote: ... Cells were incubated with 100 μg.mL−1 RNase A and 15 μg.mL−1 propidium iodide (PI) for 30 min before PI was detected using the BD FACSCalibur™ (BD Biosciences). Data was analysed using FlowJo_V10™ software (FlowJo ...
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bioRxiv - Cell Biology 2019Quote: ... 1-2×106 cells were incubated for 15 min at +4°C with blocking buffer (1xPBS, 1% BSA, 2mM EDTA) containing 5mg/ml α-CD16/CD32 (2.4G2, BD Biosciences). For surface staining ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed once with FACS buffer and fixed with 2% paraformaldehyde for 1 h prior to analyzing on an LSRII flow cytometer (BD Biosciences). In some experiments ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining of IL-2 followed the same protocol as that for CTLA-4 but included the addition of 1 μg/mL GolgiPlug (BD Biosciences) to the culture media 12 hours prior to sample preparation ...
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bioRxiv - Immunology 2020Quote: The cell supernatant was collected after centrifugation of the cells and the secreted IL2 in the diluted cell culture medium (1:4 dilution) was measured using the IL2 Flex set cytometric bead array (CBA) (BD, 558270) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were processed as previously described47 and 1-2 million cells were injected into cleared mammary fat pads in 10-20 microliters of commercial Matrigel (BD Biosciences). For ER+ tumors ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed once using PBS with 1% BSA and kept on ice until FACS sorting using the FACSAria III (BD Biosciences). For RNA isolation ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then grown for 3 d and live cells were labeled with CD44-Alexa-Fluor-647 or TfR1-PE antibodies in 2% FBS/1× PBS with 1 mM ETDA and subsequently CD44-negative or TfR1-negative cells were sorted by FACS (Aria IIu, BD Biosciences), seeding one cell per well into 96-well plates ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were stained by replacing the solution containing ethanol with another made up of PBS/FBS and implemented with PI (1:100, BD Bioscience) and RNaseA (1:400 ...
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bioRxiv - Microbiology 2020Quote: ... Spore suspensions were prepared by plating a 1:100 dilution of an overnight culture onto a 70:30 medium (63 g Bacto peptone (BD Difco), 3.5 g proteose peptone (BD Difco) ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturer’s protocol and intracellular staining was performed using anti-BCL-6-AF647 (clone K112-91, 1:100, BD, cat#561525).