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Citations for Becton, Dickinson and Company :
5101 - 5150 of 5708 citations for Herpes Simplex Virus 1 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were incubated for 15 minutes at 4°C with anti CD16/CD32 Mouse BD Fc Block diluted 1/50 (BD) in 100 µL of FACS buffer in 96 wells round-bottom plates (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... Purity > 97% of CD14+ monocytes after magnetic sorting was verified by flow cytometry (anti-CD14-FITC clone MφP9, 1:40, #345784; BD Biosciences). For macrophage differentiation ...
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bioRxiv - Immunology 2024Quote: ... The cells were resuspended in 1% bovine serum albumin (BSA) containing PBS and analyzed with a FACSCanto II (BD Biosciences) or an LSR Fortessa (BD Biosciences ...
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bioRxiv - Developmental Biology 2024Quote: ... Both yolk sac tissues and whole embryos were incubated in primary antibody to rat anti-PECAM-1(BD Pharmingen, 553370) diluted in CAS-Block overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: The primary antibodies and corresponding dilutions used are as follows: (1) anti-CtBP2 mouse monoclonal antibody IgG1 (BD Transduction Laboratories) at 1:200 ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice with PBS and incubated for 20 minutes on ice with 1:100 dilution anti-CD16/32 (clone 2.4G2, BD biosciences) and 1:500 dilution Live/Dead nearIR dye (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by stimulation for the appropriate time at 37°C with pre-warmed anti-hamster IgG1 antibody (5µg ml-1; G94-56, BD Pharmingen). Ex vivo expansion of B cells was performed using recombinant murine IL-4 (100 u ml-1 ...
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bioRxiv - Biochemistry 2024Quote: ... The 1 mM Rhobo6 solution was then injected retro-orbitally using a 0.5 mL tuberculin syringe with 27G needle (BD, 305620). Mice were allowed to recover in their cage over 30 mins then euthanized by cervical dislocation ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were pelleted and resuspended in PBS with DAPI (1:3000) and analysed with a BD FACS Canto (BD Biosciences) analyser ...
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bioRxiv - Cell Biology 2024Quote: ... monoclonal mouse anti-human CD61 BV605 conjugated antibody and monoclonal anti-human PAC-1 FITC antibody were obtained from BD Bioscience (Franklin Lakes ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibodies for immunostaining were diluted in PBS with 3% BSA and 0.1% Tween-20 as follows: 1:800 rat anti-mouse CD31 (BD, 553370), 1:1000 rabbit anti-ERG (Abcam ...
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bioRxiv - Immunology 2024Quote: ... diluted with fluorescence-activated cell sorting (FACS) buffer (PBS containing 0.1% sodium azide and 1% bovine serum albumin) and Brilliant Stain Buffer (BD Biosciences). The cells were washed in FACS buffer and fixed in PBS containing 4% paraformaldehyde (Electron Microscopy Sciences) ...
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bioRxiv - Immunology 2024Quote: ... MD4 and Ars/A1-derived antibody secreting cells were detected with Biotin-conjugated Mouse Anti-Mouse IgM[a] (Clone:DS-1; BD Biosciences), followed by Streptavidin-AP (SouthernBiotech) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were resuspended in 500 m 2% FBS in HBSS and incubated on ice in the dark with 1 l Fc block (BD Biosciences) for 3 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... PDX derived cells were centrifuged and suspended in ALDEFLUOR assay buffer containing Fixable Viability Stain 660 (FVS660, 1:1000, BD Horizon) after incubation at 37°C ...
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bioRxiv - Systems Biology 2020Quote: ... White cells were resuspended in PBS supplemented with 1% human serum and 2 mM EDTA and analyzed on an LSR Fortessa instrument (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... 1×105 Tconv cells were co-cultured with Treg cells at different ratios in the presence of 2×105 irradiated APCs and 1 µg/ml soluble anti-CD3 antibody (554829, BD Biosciences). The cells were cultured in T-cell complete media which consists of RPMI 1640 medium supplemented with 50 U/ml penicillin ...
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bioRxiv - Immunology 2021Quote: ... for 5 minutes prior to staining at 4°C in the dark using the antibodies listed in supplementary table 1 and analysed using an LSR Fortessa cytometer (BD Biosciences) and FlowJo software (BD) ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight in blocking solution with the following primary antibodies (1:100): rat anti-mouse SiglecF (E50-2440, 552126 BD Biosciences), Armenian hamster anti-mouse CD80 (16-10A1 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated with pS6 or HK2 antibody for 1 h and O-GlcNAc antibody for 20 min at RT in the dark in cytoplasmic permeabilization buffer (BD Biosciences). Nuclear permeabilization buffer was used for c-Myc staining ...
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bioRxiv - Immunology 2021Quote: ... and surface stained for 30 mins at 4°C with 50 μL/well anti-mouse CD3 APC-CY7 (clone 17A2, 1:400, BD Biosciences), anti-mouse CD4 BV510 (clone RM4-5 ...
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bioRxiv - Immunology 2021Quote: ... 10% FCS and incubated with the indicated pool of peptides (5 μg/ml final concentration of each peptide) and brefeldin A (1 μg/ml; BD Pharmingen) at 37°C for 12-16 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... run on PAGE together with input sample (1:20 of amount of immunoprecipitated proteins) and blotted with anti-PARP1 (Mouse Mab 551025 BD Pharmigen), anti-TRF1 (Rabbit Pab sc-6165 ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed once with FACS buffer and then stained with surface antibodies diluted 1:200 in Brilliant buffer (BD Biosciences) for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were re-suspended in 100 μl of FCSB and 1 μl of mouse anti-human CD10-PE (561002, BD Biosciences) and mouse anti-human CD92-AF647 (565316 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Three stable cell lines expressing shRNAs were cultured simultaneously under 1% hypoxia and 21% normoxia for 48 hours and collected for FITC-conjugated annexin V (BD Pharmingen) staining followed by apoptotic cell number analysis by flow cytometry.
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bioRxiv - Immunology 2022Quote: BMDMs were harvested as described before and Fc receptors were blocked with an anti-mouse CD16/CD32 antibody (1:250, BD Biosciences) in FACS buffer (5 % heat-inactivated FCS ...
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bioRxiv - Immunology 2022Quote: ... 1–3 ×106 PBMC were stained with a saturating concentration of the following antibodies: CD3 AF700 (clone SP34-2, BD Biosciences), CD4 eFluor450 (clone OKT4 ...
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bioRxiv - Neuroscience 2022Quote: Dissociated and resuspended iTF-Microglia were blocked for 15 min with 1:20 Human FC Block (BD Biosciences; Cat. No. 564220) and then stained with 1:66 PE/Cy7 anti-human CD184 (CXCR4 ...
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bioRxiv - Microbiology 2022Quote: ... BW25113ΔompT (JW0554) and BW25113ΔeptA (JW5730) (Supplementary Table 1) were routinely grown aerobically in Difco Antibiotic Medium 3 (BD, Franklin Lakes, USA) at 37 °C degrees without agitation ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... and with hematopoietic stem cell markers APC-conjugated anti-CD117 (c-Kit) and PE-conjugated Ly6A/E (Sca-1) (BD Biosciences). Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C with the astrocyte (mouse anti-GFAP; 1:1000, BD Biosciences, San Diego, CA, USA) and microglia (rat anti-CD11b ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μl of platelet suspension was mixed with 40 μl of staining solution (HEPES-Tyrode buffer supplemented with 2.5 mM CaCl2, 1:50 PE rat anti-mouse CD41 antibody (BD Biosciences 561850), and 3:50 FITC Annexin V (BD Biosciences 556419)) ...
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bioRxiv - Cell Biology 2022Quote: ... were loaded onto SDS-PAGE gel and immunoblotted using antibodies against clathrin heavy chain (1:1,000; BD Bioscience, San Jose, CA), dynamin (1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were re-stimulated with 1 µg/mL of master peptide pools in the presence of brefeldin A (GolgiPlug; BD Bioscience) and monensin (GolgiStop ...
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bioRxiv - Immunology 2022Quote: ... NK cells were added to the washed ELISA plate and incubated together with anti-human CD107a (BD, 1:40, clone: H4A3), brefeldin A (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... stained with anti-CD117-PECy7 mAb (5 μg.mL−1) and viability dye and sorted by a FACSMelody™ cell sorter (BD biosciences). For immunofluorescence experiments ...
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bioRxiv - Immunology 2021Quote: ... TNFα and CD107a expression by multicolor flow cytometry panel (all antibodies used are listed in Table 1) in a BD LSR Fortessa Instrument (BD Biosciences). Polyfunctionality was evaluated using Boolean gating obtained with FlowJo v10 software.
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bioRxiv - Cancer Biology 2019Quote: ... MDA-MB-231 parental or HSF1 KO with either scrambled control or DYRK2 knock-down shRNA (1 or 2) were counted and suspended in sterile phosphate buffer saline containing 50% Matrigel (BD Bioscience). 300,000 cells (100 μl ...
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bioRxiv - Cell Biology 2019Quote: ... transferred to flow cytometry tubes and stained with 1:100 mouse IgG2a CD31-647 clone M89D3 (BD Bioscience, 558094, lot 8145771) for 30 min on ice then washed once with DPBS ...
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bioRxiv - Cell Biology 2019Quote: ... Cells expressing desired transgenes were isolated by culturing in growth media supplemented with puromycin (1 μg/mL) or using fluorescence-activated cell sorting (FACS) as appropriate (FACSAria2 or FACSAria3; BD Biosciences).
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bioRxiv - Cell Biology 2019Quote: ... Tet1KO and WT reprogramming intermediates were incubated 30 min on ice with Phycoerythrin (PE)-conjugated Mouse anti SSEA1 (1:100, Cat.#FAB2155P-025, R&D) and analysed on CANTO I (BD Biosciences). PGCLCs at day8 were sorted using FITC Mouse anti-SSEA1 (1:20 ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were resuspended with the 1× Binding Buffer and incubated with 5μL FITC-conjugated annexin V Annexin FITC (BD Biosciences, USA) and 5μL PI (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: Lymphocytes or purified splenic B cells were washed in PBS with 1% BSA and incubated with 1mg/ml purified rat anti-mouse CD16/CD32 (Fc block, BD Pharmingen) at 4°C for 15 min ...
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bioRxiv - Immunology 2019Quote: ... incubated with anti-mouse IgG1-fluorescein isothiocyanate for 45 min at room temperature (1:100 dilution; BD Pharmingen; cat. no. 553443), and washed three times with PBS ...
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bioRxiv - Immunology 2019Quote: ... stained with cocktails of fluorochrome–conjugated antibodies (Supplemental Table 3), and acquired on LSR II (Cohort 1) or CANTO II (TT0, T6M) flow cytometers using FACS DIVA software (BD Biosciences). All flow cytometry data were analyzed with FlowJo (TreeStar Software ...
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bioRxiv - Immunology 2019Quote: ... cells were cultured in the presence of 1 μg/mL Brefeldin A for indicated timepoints and were fixed and permeabilized with Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei enriched in Nuclei Suspension Buffer were stained with DAPI (1:1000) for nuclei isolation using the FACSAria™ III cell sorter (BD Biosciences ...
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bioRxiv - Biochemistry 2019Quote: ... and four patients with cardiac comorbidities including severe congestive cardiac failure (1 male, 3 female, 29-59 years old) was drawn into ACD-A tubes (BD Vacutainer) and plasma collected by twice centrifugation at 800 g for 20 min at room temperature ...
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bioRxiv - Immunology 2019Quote: ... The cells were then aliquoted into FACS tubes (0.25-1×106 cells/tube) with strainer caps (BD Biosciences, San Jose, CA).