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Citations for Becton, Dickinson and Company :
5151 - 5200 of 5708 citations for Herpes Simplex Virus 1 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... We then double sorted lineage depleted cells for an HSC-enriched population (Lin- Sca-1+c-Kit+CD34+-CD135-) into 2% FBS in HBSS using fluorescent activated cell sorting (FACS) (BD Aria). Recipient mice ...
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bioRxiv - Cancer Biology 2019Quote: ... Apoptosis in cell lines and CLL cells was measured by Annexin staining and flow cytometry using the FITC Annexin V Apoptosis Detection Kit 1 (BD Pharmingen). Percentage apoptosis represents the % of apoptotic cells above the % apoptosis in control cells ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were then incubated with fluorophore conjugated primary antibodies for 1 hr in the dark (Alexa 488 Vimentin Antibody (BD, #562338) and Alexa 647 E-Cadherin Antibody (BD ...
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bioRxiv - Cell Biology 2019Quote: ... cells were plated at 1/6 confluence in either six 175 cm2 flasks or in two three-layer 525 cm2 tissue culture flasks (BD Biosciences) and after 24 hours synchronized at different stages of the cell cycle as described in the previous section ...
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bioRxiv - Developmental Biology 2019Quote: ... The precipitated cells were washed two times with 2% FBS/PBS and finally resuspended in 1% FBS/PBS for sorting of the GFP-positive PGCs by using a flow cytometry (FACSVerse, BD Biosciences). The GFP-negative somatic cells and GFP-positive PGCs after sorting were subject to evaluation of mutation efficiencies.
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bioRxiv - Microbiology 2019Quote: ... Cells were then washed three times in PBS followed by incubation in 1% Human Fc Block (BD Biosciences, San Jose, CA) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... cells were stained with a 1:50 dilution of phycoerythrin (PE)-conjugated mouse monoclonal antibody against human TRAIL (550516, BD Biosciences). For immunophenotyping of MSCs the cells were stained with 1:50 dilution of fluorochrome-conjugated mouse monoclonal antibodies against CD73 (PE-Vio770 ...
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bioRxiv - Immunology 2021Quote: ... Bulk of primary cells (either from the spleen or the lungs) were then rechallenge in vitro with YAK-1 cells for 4 hours in presence of monensin and brefeldin (BD Bioscience) at the Effector/Target ratio of 5 ...
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bioRxiv - Immunology 2021Quote: ... labelled with anti-human myeloid and stromal markers (Table 1) and analysed by flow cytometry on a LSR Fortessa (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Total T cells (1 × 106) were activated with plate bound α-CD3ε (OKT3, Biolgend) plus α-CD28 (CD28.2 RUO, BD Biosciences) at 5µg/mL each or corresponding isotype controls for a total of 72 hours ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with a panel of antibodies (Supplementary Table 1) in flow cytometry staining buffer with BD Horizon Brilliant Stain Buffer Plus (BD Biosciences) for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed in flow buffer and fixed in flow buffer plus 1% PFA before filtering through 40 μM mesh strainer/tube (BD Biosciences). Flow cytometry was performed on the BD FACSCelesta cytometer and data was analyzed using FlowJo Version 10 (Becton ...
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bioRxiv - Bioengineering 2020Quote: ... cells were then suspended in alginate precursor solution ‘A’ at 5 x 106 cells/mL (final concentration 2.5 x 106 cells/mL) and loaded into 1 or 3 mL syringes (BD luer lock). Following previous protocols ...
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bioRxiv - Neuroscience 2020Quote: ... The pellet was recovered and sequentially incubated with anti-mouse CD16/CD32 (1:250, BD Pharmingen; for 15min at 4°C) followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200, BD Pharmingen; for 30min at 4°C), all in 2% BSA ...
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bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
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bioRxiv - Neuroscience 2021Quote: ... Fc receptors were blocked as described above and cells were stained with CD3e FITC (1:200, clone 145-2C11, BD Pharmingen) or CD3e PE-Cy7 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... The pellet was resuspended in 1 ml of FACS buffer and analysis was performed on a LSRII-U flow cytometer (BD Biosciences). Single color controls were used to determine proper gating prior to sample analysis.
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bioRxiv - Neuroscience 2021Quote: ... Tissues were blocked with 5% donkey serum and 0.1% Triton X-100 in PBS for one hour at room temperature and incubated with 1:1000 of anti-CD47 (BD Biosciences), 1:500 of anti-TSP1 (Abcam) ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used for analysis of the H2B-GFP LR population (Figure 5): rat anti- Cd74-BUV395 (Clone In-1, BD, 740274), rat anti-Cd45-BUV563 (Clone 30-F11 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed with PBS containing 5 mM EDTA and 3% FBS (FACS Buffer) and then blocked with 1% anti-mouse CD16/CD32 (BD biosciences), 5% normal rat serum and 5% normal mouse serum in FACS buffer for 10 minutes at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Discs were then washed three times for 10 minutes each in 0.1% PBST and incubated overnight at 4°C in mouse anti-BrdU primary antibody (B44) (BD, 1:50). The next day ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins of interest were visualized after incubation with primary antibodies (α-synuclein 1:1000 BD Biosciences (San Jose, CA, USA) #610787 ...
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bioRxiv - Plant Biology 2021Quote: ... and diluted to a final concentration of 1.5 x 105 spores ml−1 in half strength potato dextrose broth (PDB ½, BD Difco™) prior to the inoculation ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were incubated for 15 minutes at room temperature in the dark in 50uL PBS with Fc Block (1/100 dilution, BD, 564220) and Zombie UV dye (1 in 500 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... at 1:1 E:T ratio at 37°C with 5% CO2 in the presence of a CD107a antibody for 1 hr before treatment with brefeldin A (10 µg/ml; BD Biosciences) and monensin (0.7µg/ml ...
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bioRxiv - Microbiology 2020Quote: ... AU) of the infected cells 1 and 3 hours after infection by flow cytometry using a FACS Aria III (BD Biosciences). The intensity of GFP fluorescence corresponds to the amount of intracellular bacteria ...
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bioRxiv - Microbiology 2020Quote: ... red blood cells in a concentration of 1 × 107 cells/mL were incubated for half an hour with 100 μl thiazole orange (TO) (Retic-Count Reagent; BD Biosciences) and Alexa-647 conjugated mouse mAb against transferrin receptor (CD71 ...
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bioRxiv - Microbiology 2020Quote: ... and combined 1:1 with Tris-Borate-EDTA buffer (TBE) at a final working concentration of 1X in 50 mL conical tubes (BD Falcon). Samples (1.0 mL in 50 mL conical tubes ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 72 h at 4°C in mouse anti-PKCδ primary antibody (1:1000, BD Biosciences, 610397) in blocking solution containing 1.5% NGS ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... for 24 h and HIV-1 reactivation was assessed by estimating GFP+ cells (excitation: 488 nm; emission: 510 nm) using BD FACSVerse flow cytometer (BD Biosciences). The data were analysed using FACSuite software (BD Biosciences).
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bioRxiv - Neuroscience 2020Quote: ... The EGFP+stop cassette was inserted in-frame into exon 1 at the AgeI site following amplification from pEGFP-C1 (BD Biosciences) using primers 2-For and 2-Rev ...
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bioRxiv - Immunology 2021Quote: ... GFP-positive transfected cells were sorted in 96-well plates at a density of 1 cell per well using a FACS ARIA III (BD Biosciences) and cultivated in THP-1 media ...
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bioRxiv - Immunology 2021Quote: ... Cells were aliquoted to a count of 1 million cells each and incubated on ice with Fc Block (BD, Table S1) at a 1:20 dilution ...
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bioRxiv - Immunology 2020Quote: ... 8.0 × 105 cells per condition were fixed in 1% PFA for 10 min at room temperature and subsequently stained with primary antibodies: mouse anti-human PEcy7-CD16 (BD #557744), mouse anti-human PerCPcy5.5-CD66b (Biolegend # 305107) ...
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bioRxiv - Genomics 2021Quote: ... the suspension was sorted by applying a 4-way purity mode based on the selected DAPI positive nuclei population (1:1000, BD Pharmingen) using forward and side scatter gating strategy (FACS) ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were run for 5 min at low flow rate (12 ± 3 μl min−1) and cytometric data were documented using BD CellQuest Pro software (BD Biosciences). In addition to glaucophyte cells ...
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bioRxiv - Systems Biology 2020Quote: ... cells were extensively washed with PBS and stained with NeutrAvidin Protein DyLight 488 1:50 for 20 min prior analysis using an Accuri C6 Flow Cytometer (BD Biosciences) with side-scatter (height ...
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bioRxiv - Microbiology 2020Quote: ... during 15 min at 4 °C in MACS buffer followed by incubation with anti-CD45 1:100 (clone 30F-11, BD biosciences), anti-EpCAM 1:100 (clone G8.8 ...
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bioRxiv - Microbiology 2020Quote: ... http://www.eucast.org) and ISO 20776-1:2006 guidelines with the exception that cation-adjusted Mueller-Hinton broth (BD, New Jersey, USA) was supplemented with 100 U of catalase (Worthington Biochemical Corporation ...
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bioRxiv - Cancer Biology 2020Quote: ... For both ACHN and CAKI-1 cell lines 2×106 cells were diluted in 50 µl of PBS and 50 µl of Matrigel (BD Biosciences) and were injected subcutaneously into the right and left flank of each mouse ...
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bioRxiv - Cancer Biology 2020Quote: ... into a 50 mL conical vial using a 10 mL serological pipette and the plunger from a 1 mL syringe (BD, 309628). Cell strainers were washed with 25 mL sterile PBS into corresponding conical vials ...
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bioRxiv - Cancer Biology 2021Quote: ... A primary antibody dilution was prepared in the blocking solution with the antibodies diluted 1:200 for HIF1α (BD Bioscience, 610958), 1:50 for phosphor-S6 (CST ...
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bioRxiv - Cell Biology 2021Quote: ... Crypts were mixed with Col6a1cre-GFP+ and GFP− IMCs sorted by FACS at passage 1 and subsequently resuspended in Matrigel (BD Biosciences) at 250 crypts/50.000 IMCs/30 μl in 48-well plates ...
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bioRxiv - Cancer Biology 2021Quote: ... Flow cytometry for negative checkpoint regulator was performed as previously described(14) using antibodies: BV510 mouse anti-human CD279 (PD1) (1:100; 563076, BD Horizon), PerCP-eFluor 710 mouse anti-human CD223 (LAG-3 ...
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bioRxiv - Cancer Biology 2021Quote: ... The levels of DNA-loaded MCM were detected by incubating cells in anti-MCM2 BM28 antibody (1:200, BD biosciences, 610700) for 1 hr at 37°C in the dark followed by incubation in anti-mouse-488 secondary antibody (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... 3 times 5 min washes with TNT were followed by a 1 h incubation with biotinylated goat anti-rat (559286, BD biosciences) 1:100 in TNB ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were activated in plates coated with anti-mouse CD3ε and anti-mouse CD28 antibodies (1 µg/ml, BD Biosciences) for up to 72h.
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bioRxiv - Immunology 2022Quote: ... into FACS buffer (1X PBS with 2% FBS and 1 mM EDTA) and analyzed with a FACS Aria flow cytometer (BD Biosciences).
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bioRxiv - Genomics 2022Quote: ... 1 μg of total RNA from NIH/3T3 cells was diluted with 500 μL of BD Rhapsody Lysis buffer (BD Biosciences) supplemented with 15 mM DTT (BD Biosciences ...