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Citations for Becton, Dickinson and Company :
5101 - 5150 of 5734 citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The eluted macrophage fraction was resuspended in a final volume of 200μl MACS buffer and incubated 10 min with Fc block (1:50, BD Pharmingen). 200μl of staining solution containing flow cytometry antibodies (anti-CD45-PECy7 (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated overnight at 4° with primary antibodies at the following dilutions: mouse anti-βII-spectrin 1:2000 (BD-bioscience), rabbit anti-βII-spectrin 1:2000 (Abcam) ...
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bioRxiv - Biophysics 2022Quote: THP-1 cells were cultured in wells as detailed in the cell culture methods and analyzed using a flow cytometer (BD Accuri C6 flow cytometer ...
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bioRxiv - Genetics 2023Quote: ... Cells were stringently sorted into an Eppendorf tube containing stem cell media with 10 μM ROCK inhibitor and CloneR2 using an FACSAria III Cell Sorter (sort speed 1; BD) on the basis of eGFP and mTagBFP2 double-positivity (using the control transfection to set gating) ...
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bioRxiv - Immunology 2023Quote: ... were incubated for 1 h at room temperature followed with another incubation of diluted HRP-conjugated streptavidin (554066, BD Biosciences) for 30 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Intracellular co-staining of cells was performed using a mix of fluorophore-labelled antibodies against pH2Ax-PerCP-Cy5.5 (1:100, #564718, S139, BD Biosciences) together with pH3-AL647 (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... CECs were incubated overnight at 4°C with primary antibodies mouse monoclonal anti-CD166 [3A6] (1:200 dilution, BD Biosciences), rabbit polyclonal anti-ZO1 (1:100 dilution ...
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bioRxiv - Immunology 2023Quote: ... and 3 μg/ml anti-IL-4 in the presence of plate bound anti-CD3ε (1 μg/ml) (BD Biosciences) and anti-CD28 (3 μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was Fc blocked before staining using 50 µL of 2X FC Block Solution (FACS Buffer, 1:125 dilution of anti-CD16/CD32 (BD) and incubated for 10 minutes on ice ...
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bioRxiv - Genomics 2023Quote: ... with the addition of 1 µg/mL doxycycline 48 hours before being screened on a BD LSRFortessa SORP (BD Biosciences) to identify colonies with high rates of both landing pad (BFP ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with 1% FBS/PBS then stained with PE/Cy7-conjugated rat anti-mouse Igκ light chain antibody (BD) for 30min at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2 ...
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bioRxiv - Immunology 2023Quote: ... and 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI) and analyzed on a BD FACS Aria III (BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... High-titer viral solutions were prepared and used for transduction into both WT and NF1 KO BT-474 cells by two rounds of infection of 4 viral particles per cell (MOI 1) followed by flow cytometry-based sorting (FACSAria cell sorter, BD). For live cell imaging ...
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bioRxiv - Molecular Biology 2023Quote: ... were coated overnight at 4 °C with 300 ng/mL mouse anti-human RIPK1 capture antibody (1:100 dilution in PBS of BD Bioscience BD610459 lot 0314064 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells 1 x 106 cells per condition were incubated with an AlexaFluor647-conjugated anti-CD271 antibody (BD Pharmingen, RRID: AB_1645403) for 1 h in the dark before being counted on a BD LSRFortessa™ Flow Cytometer (BD Biosciences-IN) ...
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bioRxiv - Neuroscience 2023Quote: ... and the cuticle between the eyes was cut with a needle (BD PrecisionGlide Hypodermic Needles 18G x 1” BD 305195) and removed with forceps ...
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bioRxiv - Molecular Biology 2023Quote: The specimens were washed in PBS three times and incubated in 1:2000 v/v DAPI (BD Bioscience, NJ, USA) in PBS solution for 5 min.
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bioRxiv - Neuroscience 2023Quote: ... All dissociated cells were blocked with Fc receptors staining buffer (1% anti-mouse CD16/CD32, 2.4 G2, 2% FBS, 5% NRS, and 2% NMS in HBSS; BD Bioscience) and then stained with a standard panel of antibodies ...
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bioRxiv - Immunology 2023Quote: Both SS1 and A61C (1) (clinical strain) were revived from glycerol stock using brain heart infusion agar (BD Difco, USA) supplemented with 7% horse blood ...
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bioRxiv - Immunology 2023Quote: ... Surface antigens were stained for 30 min at 4°C with indicated antibodies (Table.1) after Fc blocking for 4°C 30 min with anti-mouse CD16/32 (BD) without wash ...
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bioRxiv - Immunology 2023Quote: ... and TG2-binding and non-TG2-binding IgA plasma cells were bulk-sorted into empty microtubes that had been coated with 1% BSA/PBS using a FACSAriaIII instrument (BD). For each of four untreated CeD patients ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were diluted in 3% BSA/PBST (0.1% Triton X-100 in PBS) and incubated with the samples at 37°C for 1 h: mouse anti-BrdU (BD Biosciences 347580 ...
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bioRxiv - Microbiology 2023Quote: ... We then create a 1 mm thick sheet of agarose using a 3 mL syringe (BD, 3 mL Luer lock) and a blunt needle (Industrial Dispensing Supplies ...
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bioRxiv - Cancer Biology 2023Quote: ... Single cells from digested xenograft tumors were labeled using a BUV395 anti-mouse cocktail (pooled and diluted 1:40: anti-mouse H2kD [BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Single cells from digested xenograft tumors were labeled using a BUV395 anti-mouse cocktail (pooled and diluted 1:40: anti-mouse H2kD [BD Biosciences, cat. 742437], anti-mouse CD45 [BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Single cells from digested xenograft tumors were labeled using a BUV395 anti-mouse cocktail (pooled and diluted 1:40: anti-mouse H2kD [BD Biosciences, cat. 742437], anti-mouse CD45 [BD Biosciences, cat. 567451], anti-mouse CD31 [BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: We used the following antibodies for immunohistochemistry (IHC) and immunoblotting (IB): Rat anti-Mouse CD31 (BD 550274; IHC 1:200), GAPDH (Cell Signaling 5174S ...
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bioRxiv - Bioengineering 2023Quote: ... Labelled cells were then washed in PBS + 1% BSA and analyzed on a FACS Canto II flow cytometer (BD Biosciences). Data analysis was performed using FlowJo software (Tree Star) ...
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bioRxiv - Molecular Biology 2023Quote: ... two-color analysis of murine or human platelet activation was performed using fluorophore-labeled antibodies for P-selectin expression (Emfret Analytics, Wug.E9-FITC, #M130-1, and BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were incubated for 15 minutes at 4°C with anti CD16/CD32 Mouse BD Fc Block diluted 1/50 (BD) in 100 µL of FACS buffer in 96 wells round-bottom plates (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... Purity > 97% of CD14+ monocytes after magnetic sorting was verified by flow cytometry (anti-CD14-FITC clone MφP9, 1:40, #345784; BD Biosciences). For macrophage differentiation ...
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bioRxiv - Immunology 2024Quote: ... The cells were resuspended in 1% bovine serum albumin (BSA) containing PBS and analyzed with a FACSCanto II (BD Biosciences) or an LSR Fortessa (BD Biosciences ...
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bioRxiv - Developmental Biology 2024Quote: ... Both yolk sac tissues and whole embryos were incubated in primary antibody to rat anti-PECAM-1(BD Pharmingen, 553370) diluted in CAS-Block overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: The primary antibodies and corresponding dilutions used are as follows: (1) anti-CtBP2 mouse monoclonal antibody IgG1 (BD Transduction Laboratories) at 1:200 ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice with PBS and incubated for 20 minutes on ice with 1:100 dilution anti-CD16/32 (clone 2.4G2, BD biosciences) and 1:500 dilution Live/Dead nearIR dye (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by stimulation for the appropriate time at 37°C with pre-warmed anti-hamster IgG1 antibody (5µg ml-1; G94-56, BD Pharmingen). Ex vivo expansion of B cells was performed using recombinant murine IL-4 (100 u ml-1 ...
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bioRxiv - Biochemistry 2024Quote: ... The 1 mM Rhobo6 solution was then injected retro-orbitally using a 0.5 mL tuberculin syringe with 27G needle (BD, 305620). Mice were allowed to recover in their cage over 30 mins then euthanized by cervical dislocation ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were pelleted and resuspended in PBS with DAPI (1:3000) and analysed with a BD FACS Canto (BD Biosciences) analyser ...
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bioRxiv - Cell Biology 2024Quote: ... monoclonal mouse anti-human CD61 BV605 conjugated antibody and monoclonal anti-human PAC-1 FITC antibody were obtained from BD Bioscience (Franklin Lakes ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibodies for immunostaining were diluted in PBS with 3% BSA and 0.1% Tween-20 as follows: 1:800 rat anti-mouse CD31 (BD, 553370), 1:1000 rabbit anti-ERG (Abcam ...
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bioRxiv - Immunology 2024Quote: ... diluted with fluorescence-activated cell sorting (FACS) buffer (PBS containing 0.1% sodium azide and 1% bovine serum albumin) and Brilliant Stain Buffer (BD Biosciences). The cells were washed in FACS buffer and fixed in PBS containing 4% paraformaldehyde (Electron Microscopy Sciences) ...
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bioRxiv - Immunology 2024Quote: ... MD4 and Ars/A1-derived antibody secreting cells were detected with Biotin-conjugated Mouse Anti-Mouse IgM[a] (Clone:DS-1; BD Biosciences), followed by Streptavidin-AP (SouthernBiotech) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were resuspended in 500 m 2% FBS in HBSS and incubated on ice in the dark with 1 l Fc block (BD Biosciences) for 3 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... PDX derived cells were centrifuged and suspended in ALDEFLUOR assay buffer containing Fixable Viability Stain 660 (FVS660, 1:1000, BD Horizon) after incubation at 37°C ...
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bioRxiv - Systems Biology 2020Quote: ... White cells were resuspended in PBS supplemented with 1% human serum and 2 mM EDTA and analyzed on an LSR Fortessa instrument (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... 1×105 Tconv cells were co-cultured with Treg cells at different ratios in the presence of 2×105 irradiated APCs and 1 µg/ml soluble anti-CD3 antibody (554829, BD Biosciences). The cells were cultured in T-cell complete media which consists of RPMI 1640 medium supplemented with 50 U/ml penicillin ...
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bioRxiv - Immunology 2021Quote: ... for 5 minutes prior to staining at 4°C in the dark using the antibodies listed in supplementary table 1 and analysed using an LSR Fortessa cytometer (BD Biosciences) and FlowJo software (BD) ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight in blocking solution with the following primary antibodies (1:100): rat anti-mouse SiglecF (E50-2440, 552126 BD Biosciences), Armenian hamster anti-mouse CD80 (16-10A1 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated with pS6 or HK2 antibody for 1 h and O-GlcNAc antibody for 20 min at RT in the dark in cytoplasmic permeabilization buffer (BD Biosciences). Nuclear permeabilization buffer was used for c-Myc staining ...