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Citations for Becton, Dickinson and Company :
5151 - 5200 of 5734 citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and surface stained for 30 mins at 4°C with 50 μL/well anti-mouse CD3 APC-CY7 (clone 17A2, 1:400, BD Biosciences), anti-mouse CD4 BV510 (clone RM4-5 ...
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bioRxiv - Immunology 2021Quote: ... 10% FCS and incubated with the indicated pool of peptides (5 μg/ml final concentration of each peptide) and brefeldin A (1 μg/ml; BD Pharmingen) at 37°C for 12-16 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... run on PAGE together with input sample (1:20 of amount of immunoprecipitated proteins) and blotted with anti-PARP1 (Mouse Mab 551025 BD Pharmigen), anti-TRF1 (Rabbit Pab sc-6165 ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed once with FACS buffer and then stained with surface antibodies diluted 1:200 in Brilliant buffer (BD Biosciences) for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were re-suspended in 100 μl of FCSB and 1 μl of mouse anti-human CD10-PE (561002, BD Biosciences) and mouse anti-human CD92-AF647 (565316 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Three stable cell lines expressing shRNAs were cultured simultaneously under 1% hypoxia and 21% normoxia for 48 hours and collected for FITC-conjugated annexin V (BD Pharmingen) staining followed by apoptotic cell number analysis by flow cytometry.
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bioRxiv - Immunology 2022Quote: BMDMs were harvested as described before and Fc receptors were blocked with an anti-mouse CD16/CD32 antibody (1:250, BD Biosciences) in FACS buffer (5 % heat-inactivated FCS ...
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bioRxiv - Immunology 2022Quote: ... 1–3 ×106 PBMC were stained with a saturating concentration of the following antibodies: CD3 AF700 (clone SP34-2, BD Biosciences), CD4 eFluor450 (clone OKT4 ...
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bioRxiv - Neuroscience 2022Quote: Dissociated and resuspended iTF-Microglia were blocked for 15 min with 1:20 Human FC Block (BD Biosciences; Cat. No. 564220) and then stained with 1:66 PE/Cy7 anti-human CD184 (CXCR4 ...
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bioRxiv - Microbiology 2022Quote: ... BW25113ΔompT (JW0554) and BW25113ΔeptA (JW5730) (Supplementary Table 1) were routinely grown aerobically in Difco Antibiotic Medium 3 (BD, Franklin Lakes, USA) at 37 °C degrees without agitation ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... and with hematopoietic stem cell markers APC-conjugated anti-CD117 (c-Kit) and PE-conjugated Ly6A/E (Sca-1) (BD Biosciences). Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C with the astrocyte (mouse anti-GFAP; 1:1000, BD Biosciences, San Diego, CA, USA) and microglia (rat anti-CD11b ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μl of platelet suspension was mixed with 40 μl of staining solution (HEPES-Tyrode buffer supplemented with 2.5 mM CaCl2, 1:50 PE rat anti-mouse CD41 antibody (BD Biosciences 561850), and 3:50 FITC Annexin V (BD Biosciences 556419)) ...
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bioRxiv - Cell Biology 2022Quote: ... were loaded onto SDS-PAGE gel and immunoblotted using antibodies against clathrin heavy chain (1:1,000; BD Bioscience, San Jose, CA), dynamin (1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were re-stimulated with 1 µg/mL of master peptide pools in the presence of brefeldin A (GolgiPlug; BD Bioscience) and monensin (GolgiStop ...
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bioRxiv - Immunology 2022Quote: ... NK cells were added to the washed ELISA plate and incubated together with anti-human CD107a (BD, 1:40, clone: H4A3), brefeldin A (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... stained with anti-CD117-PECy7 mAb (5 μg.mL−1) and viability dye and sorted by a FACSMelody™ cell sorter (BD biosciences). For immunofluorescence experiments ...
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bioRxiv - Immunology 2021Quote: ... TNFα and CD107a expression by multicolor flow cytometry panel (all antibodies used are listed in Table 1) in a BD LSR Fortessa Instrument (BD Biosciences). Polyfunctionality was evaluated using Boolean gating obtained with FlowJo v10 software.
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bioRxiv - Cancer Biology 2019Quote: ... MDA-MB-231 parental or HSF1 KO with either scrambled control or DYRK2 knock-down shRNA (1 or 2) were counted and suspended in sterile phosphate buffer saline containing 50% Matrigel (BD Bioscience). 300,000 cells (100 μl ...
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bioRxiv - Cell Biology 2019Quote: ... transferred to flow cytometry tubes and stained with 1:100 mouse IgG2a CD31-647 clone M89D3 (BD Bioscience, 558094, lot 8145771) for 30 min on ice then washed once with DPBS ...
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bioRxiv - Cell Biology 2019Quote: ... Cells expressing desired transgenes were isolated by culturing in growth media supplemented with puromycin (1 μg/mL) or using fluorescence-activated cell sorting (FACS) as appropriate (FACSAria2 or FACSAria3; BD Biosciences).
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bioRxiv - Cell Biology 2019Quote: ... Tet1KO and WT reprogramming intermediates were incubated 30 min on ice with Phycoerythrin (PE)-conjugated Mouse anti SSEA1 (1:100, Cat.#FAB2155P-025, R&D) and analysed on CANTO I (BD Biosciences). PGCLCs at day8 were sorted using FITC Mouse anti-SSEA1 (1:20 ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were resuspended with the 1× Binding Buffer and incubated with 5μL FITC-conjugated annexin V Annexin FITC (BD Biosciences, USA) and 5μL PI (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: Lymphocytes or purified splenic B cells were washed in PBS with 1% BSA and incubated with 1mg/ml purified rat anti-mouse CD16/CD32 (Fc block, BD Pharmingen) at 4°C for 15 min ...
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bioRxiv - Immunology 2019Quote: ... incubated with anti-mouse IgG1-fluorescein isothiocyanate for 45 min at room temperature (1:100 dilution; BD Pharmingen; cat. no. 553443), and washed three times with PBS ...
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bioRxiv - Immunology 2019Quote: ... stained with cocktails of fluorochrome–conjugated antibodies (Supplemental Table 3), and acquired on LSR II (Cohort 1) or CANTO II (TT0, T6M) flow cytometers using FACS DIVA software (BD Biosciences). All flow cytometry data were analyzed with FlowJo (TreeStar Software ...
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bioRxiv - Immunology 2019Quote: ... cells were cultured in the presence of 1 μg/mL Brefeldin A for indicated timepoints and were fixed and permeabilized with Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei enriched in Nuclei Suspension Buffer were stained with DAPI (1:1000) for nuclei isolation using the FACSAria™ III cell sorter (BD Biosciences ...
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bioRxiv - Biochemistry 2019Quote: ... and four patients with cardiac comorbidities including severe congestive cardiac failure (1 male, 3 female, 29-59 years old) was drawn into ACD-A tubes (BD Vacutainer) and plasma collected by twice centrifugation at 800 g for 20 min at room temperature ...
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bioRxiv - Immunology 2019Quote: ... The cells were then aliquoted into FACS tubes (0.25-1×106 cells/tube) with strainer caps (BD Biosciences, San Jose, CA).
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bioRxiv - Immunology 2019Quote: ... We then double sorted lineage depleted cells for an HSC-enriched population (Lin- Sca-1+c-Kit+CD34+-CD135-) into 2% FBS in HBSS using fluorescent activated cell sorting (FACS) (BD Aria). Recipient mice ...
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bioRxiv - Cancer Biology 2019Quote: ... Apoptosis in cell lines and CLL cells was measured by Annexin staining and flow cytometry using the FITC Annexin V Apoptosis Detection Kit 1 (BD Pharmingen). Percentage apoptosis represents the % of apoptotic cells above the % apoptosis in control cells ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were then incubated with fluorophore conjugated primary antibodies for 1 hr in the dark (Alexa 488 Vimentin Antibody (BD, #562338) and Alexa 647 E-Cadherin Antibody (BD ...
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bioRxiv - Cell Biology 2019Quote: ... cells were plated at 1/6 confluence in either six 175 cm2 flasks or in two three-layer 525 cm2 tissue culture flasks (BD Biosciences) and after 24 hours synchronized at different stages of the cell cycle as described in the previous section ...
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bioRxiv - Developmental Biology 2019Quote: ... The precipitated cells were washed two times with 2% FBS/PBS and finally resuspended in 1% FBS/PBS for sorting of the GFP-positive PGCs by using a flow cytometry (FACSVerse, BD Biosciences). The GFP-negative somatic cells and GFP-positive PGCs after sorting were subject to evaluation of mutation efficiencies.
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bioRxiv - Microbiology 2019Quote: ... Cells were then washed three times in PBS followed by incubation in 1% Human Fc Block (BD Biosciences, San Jose, CA) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... cells were stained with a 1:50 dilution of phycoerythrin (PE)-conjugated mouse monoclonal antibody against human TRAIL (550516, BD Biosciences). For immunophenotyping of MSCs the cells were stained with 1:50 dilution of fluorochrome-conjugated mouse monoclonal antibodies against CD73 (PE-Vio770 ...
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bioRxiv - Immunology 2021Quote: ... Bulk of primary cells (either from the spleen or the lungs) were then rechallenge in vitro with YAK-1 cells for 4 hours in presence of monensin and brefeldin (BD Bioscience) at the Effector/Target ratio of 5 ...
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bioRxiv - Immunology 2021Quote: ... labelled with anti-human myeloid and stromal markers (Table 1) and analysed by flow cytometry on a LSR Fortessa (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Total T cells (1 × 106) were activated with plate bound α-CD3ε (OKT3, Biolgend) plus α-CD28 (CD28.2 RUO, BD Biosciences) at 5µg/mL each or corresponding isotype controls for a total of 72 hours ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with a panel of antibodies (Supplementary Table 1) in flow cytometry staining buffer with BD Horizon Brilliant Stain Buffer Plus (BD Biosciences) for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed in flow buffer and fixed in flow buffer plus 1% PFA before filtering through 40 μM mesh strainer/tube (BD Biosciences). Flow cytometry was performed on the BD FACSCelesta cytometer and data was analyzed using FlowJo Version 10 (Becton ...
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bioRxiv - Bioengineering 2020Quote: ... cells were then suspended in alginate precursor solution ‘A’ at 5 x 106 cells/mL (final concentration 2.5 x 106 cells/mL) and loaded into 1 or 3 mL syringes (BD luer lock). Following previous protocols ...
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bioRxiv - Neuroscience 2020Quote: ... The pellet was recovered and sequentially incubated with anti-mouse CD16/CD32 (1:250, BD Pharmingen; for 15min at 4°C) followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200, BD Pharmingen; for 30min at 4°C), all in 2% BSA ...
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bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
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bioRxiv - Neuroscience 2021Quote: ... Fc receptors were blocked as described above and cells were stained with CD3e FITC (1:200, clone 145-2C11, BD Pharmingen) or CD3e PE-Cy7 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... The pellet was resuspended in 1 ml of FACS buffer and analysis was performed on a LSRII-U flow cytometer (BD Biosciences). Single color controls were used to determine proper gating prior to sample analysis.
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bioRxiv - Neuroscience 2021Quote: ... Tissues were blocked with 5% donkey serum and 0.1% Triton X-100 in PBS for one hour at room temperature and incubated with 1:1000 of anti-CD47 (BD Biosciences), 1:500 of anti-TSP1 (Abcam) ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used for analysis of the H2B-GFP LR population (Figure 5): rat anti- Cd74-BUV395 (Clone In-1, BD, 740274), rat anti-Cd45-BUV563 (Clone 30-F11 ...