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Citations for Becton, Dickinson and Company :
4901 - 4950 of 5734 citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... FCRL6+/FCRL6- pro B (AA4.1+Lin-CD43+B220+CD19-IgM-) cells from the FL and BM were sorted using a FACSAria II sorter (BD).
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bioRxiv - Immunology 2019Quote: ... Single suspensions of FL cells were prepared by crushing the tissue with a 1 ml syringe plunger and passage through a 40 μm strainer (BD). Single cell suspensions were treated with ACK lysing buffer and re-suspended with PBS containing 2% FCS ...
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bioRxiv - Immunology 2019Quote: ... Cell surface markers were immunolabeled with anti-Gr-1 (PE Rat Anti-Mouse Ly6G and Ly6C, BD Pharmingen, Franklin, NJ), F4/80 (Anti-F4/80 PE ...
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bioRxiv - Genetics 2019Quote: ... a second set of antibodies was used (volume per 1×10^6 cells, all from BD Biosciences, unless stated otherwise): CD45 V500 (4μl ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed 3 times with PBS-T and 50μl of either biotinylated rat anti-mouse IgG1 (1:500, BD Bioscience), rat anti-mouse IgG2a (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Tumor xenografts were established in 6-week-old NOD SCID mice via subcutaneous injection into the dorsal region of each mouse with 1 x 107 cells/0.15 ml Hank’s balanced salt solution containing Matrigel® (BD Biosciences). Experiments were performed at five mice in each group (vector control ...
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bioRxiv - Cell Biology 2020Quote: ... at 3:100 or phospho-AKT (pS473) at 1:20 dilutions for 30 mins at RT before analysis on FACSAria II (BD).
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bioRxiv - Cancer Biology 2019Quote: ... the apoptosis of PANC-1 cells was determined by using the Annexin V-FITC/PI apoptosis detection kit (BD Biosciences). The cells were harvested with trypsin-EDTA (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... and microglia (CD45med CD11b+were sorted into collection tubes coated with FCS and filled with complete RPMI 1640 medium (1% penicillin, streptomycin, 0.1% 2-ME) with 20% FCS using a FACSAria device (BD Biosciences). Purity of sorted populations was routinely above 95% ...
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bioRxiv - Developmental Biology 2021Quote: ... Phenotyping BCAMhi and BCAMlo organoids: Single cells were incubated with anti-human BCAM BV421 (clone B64; 1:50; BD Biosciences), EGFR BV605 ...
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bioRxiv - Cell Biology 2021Quote: Human THP-1 cells (NCTC #88081201) were maintained or passaged in 25 or 75 cm2 tissue culture flasks (BD Biosciences) using RPMI-1640 (Lonza ...
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bioRxiv - Bioengineering 2020Quote: ... we preincubated 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were activated by seeding in 96-well plates pre-coated with anti-mouse CD3e (1 μg/ml working concentration, Clone: 145-2C11, BD) and anti-mouse CD28 (2 μg/ml working concentration ...
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bioRxiv - Bioengineering 2021Quote: ... at 2×106 cells/ml in wells coated with anti-mouse CD3e (1 μg/ml, Clone: 145-2C11, BD Pharmingen).
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bioRxiv - Bioengineering 2021Quote: ... Cells were plated at a concentration of 150,000 cells per 6-well in mTeSR-1 with 10 uM Y27632 (BD Biosciences). The next day ...
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bioRxiv - Biochemistry 2020Quote: ... The resins were pre-treated with yeast extract solution (0.1 g/100 ml H2O for 1 hour; BD Biosciences, NJ) to avoid non-specific binding of EVs to resins ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1% TritonX100 in PBS for 20 min at RT and stained overnight with rat-anti-PECAM1/CD31 antibodies (1/100, clone Mec13.3, BD Pharmingen) in blocking solution and secondary Alexa594 goat anti-rat IgG antibodies and DAPI (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were further incubated for 1 hr in solution containing goat anti-rabbit antibody conjugated with FITC (BD Biosciences, USA). All steps were carried out at room temperature ...
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bioRxiv - Systems Biology 2021Quote: ... The concentration of the cell suspension was adjusted to 2×106 cells ml-1 and subsequently aspirated into a 3 ml luer-lock syringe (BD).
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bioRxiv - Systems Biology 2021Quote: ... Similarly detached hCMEC/D3 cells were incubated with APC-conjugated mouse monoclonal anti-BCRP (1:100, BD Biosciences, Oxford, UK), or PE-conjugated mouse monoclonal anti-MDR1A (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were fixed with ice-cold 1% paraformaldehyde (PFA) in PBS and analyzed via flow cytometry using an LSRII SORP flow cytometer (BD) and DIVA software (BD).
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4°C with primary antibodies (Guinea-pig anti-VGAT, Synaptic Systems 131004, 1μg/mL, 1:200 and mouse anti-mGluR1, BD Pharmingen,0.5mg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Immunocytochemical staining using a rat monoclonal anti-mouse CD31 antibody (1:100; clone MEC 13.3; BD Pharmingen, San Jose, CA) was used to detect endothelial cells in the mouse heart ...
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bioRxiv - Immunology 2019Quote: ... Each obtained monomeric H-2Db/peptide complex (0.5 mg/ml) was tetramerized at a 4:1 ratio with streptavidin-PE (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... lung single cell suspensions were stained with tetramer prior to the surface and intracellular staining for 1 hour at 37°C.Intracellular cytokine staining was performed using the BD Cytofix/Cytoperm kit (BD Biosciences) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... FBS and stained on ice for 1 hr with the panel of fluorescent antibodies against IgM ([clone G20-127] BD), CD4 ([clone OKT-4] BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... buffer (PBS containing 0.1% sodium azide and 1% bovine serum albumin) and fixed/permeabilized using a Cytofix/Cytoperm Buffer Kit (BD Biosciences) or a FoxP3 Transcription Factor Buffer Kit (eBioscience) ...
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bioRxiv - Microbiology 2021Quote: ... Pellets were resuspended in 300µL of PBS + 1% v/v FBS and 150µL were added to 2µL of Mouse BD Fc Block (BD Biosciences 553142). Homogenates in Fc block were incubated for 15 minutes at 4°C ...
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bioRxiv - Genomics 2020Quote: Primary human bronchial epithelial basal cells (Passage 1 or 2) were expanded on flasks coated with collagen I (BD Biocoat) in bronchial epithelial growth medium (BEGM) ...
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bioRxiv - Cancer Biology 2020Quote: ... Sections were then fixed in 4% PFA and incubated with primary antibodies: anti-CD31 (1:50, BD Pharmigen; RRID: AB_393571), anti-CD42b (1:75 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation in blocking buffer (1% anti-mouse-CD16/CD32, 2.4 G2, 2% FBS, 5% NRS,1% triton x100 and 2% NMS in HBSS; BD Bioscience) for 1h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Fc-blocked (1 μg/mL; eBiosciences) BALF cells were stained with anti-mouse SiglecF-PE (0.3 μg/mL; BD Pharmingen), CD11c-APC (0.3 μg/mL ...
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bioRxiv - Immunology 2022Quote: For RNAseq analysis BM cells were isolated by flushing the long bones with FACS buffer (2mM EDTA, 1% FCS, PBS) and treated with Pharm LyseTM buffer (BD). Cells were enriched by magnetic removal of CD11b+ and CD19+ cells (EasySepTM ...
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bioRxiv - Microbiology 2022Quote: 5 μl of overnight bacterial suspension (~ 1×108 cells) was plated on Columbia Agar with 5% Sheep Blood purchased from BD (Becton ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were blocked with 5% milk in TBS 0.1% Tween 20 and incubated with: mouse anti-P-cad (1:2500 BD, 610228), mouse anti-MRTF-A (1:1000 Santa Cruz ...
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bioRxiv - Molecular Biology 2022Quote: ... in PBS for 60 min at RT and incubation with primary antibodies (mouse anti-RAN, 1:500, BD-610340, BD Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by blocking with 3 % normal goat serum (NGS) in PBS for 60 min at RT and incubation with primary antibodies (mouse anti-RAN, 1:500, BD-610340 ...
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bioRxiv - Immunology 2022Quote: ... Pellets were resuspended in 50 µL cold PBS 0.25% BSA with PE-conjugated mouse anti-human IgG (1:100, BD Biosciences), or PE-conjugated goat-anti human IgA (1:800) ...
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bioRxiv - Immunology 2022Quote: ... B cells were also stained with following anti-human antibodies (all at 1:200 dilution): anti-IgD-BV650 (BD, 740594), anti-CD27-BV786 (BD biosciences ...
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bioRxiv - Systems Biology 2022Quote: ... the cell pellet resuspended in 1 ml aCSF containing 0.5% BSA and the cell suspension transferred to a round bottom tube (BD Biosciences) for flow cytometry ...
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bioRxiv - Molecular Biology 2022Quote: ... expanded for 1 week and then sorted for medium-brightness ones with a BD FACSAria III Cell Sorter (BD, USA). The proliferation rates of HEK293T control cells or cells expressing G-Flamp1 were measured using the Enhanced Cell Counting Kit-8 (Cat ...
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bioRxiv - Neuroscience 2022Quote: ... with 0.2% Triton X-100 supplemented with 10% FBS and then incubated overnight at 4 °C with primary antibodies (1:100 mouse anti-ASCL1, BD 556604 ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with primary (overnight at 4 °C) and secondary (1h at room temperature) antibodies (1:100 mouse anti-ASCL1, BD 556604 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with one of four antibody panels (Supplementary Table 1) assessing different immune populations and analysed by flow cytometry (4-laser LSR Fortessa, BD). Raw FCS files were analysed using FlowJo (v10.6.2 ...
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bioRxiv - Immunology 2022Quote: Ex vivo isolated T cells and in vitro-cultured T cells were incubated with Fc-block (1:250; BD Biosciences) followed by surface staining ...
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bioRxiv - Cancer Biology 2022Quote: ... Surface staining was performed by incubating the cell suspension with 1 μg/mL purified anti-CD16/32 (2.4G2, BD Biosciences) for 10 min at 4 °C followed by an additional 20 min incubation with appropriate dilution of the surface marker antibodies ...
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bioRxiv - Biophysics 2022Quote: ... mouse anti-flotillin-1 mAb (catalog number 610820) and mouse anti-calnexin mouse mAb (catalog number 610523) were purchased from BD Transduction Laboratories (Torrey Pines ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse cells were incubated with mouse BD Fc BlockTM containing purified anti-mouse CD16/CD32 mAb 2.4G2 at 1:400 (553142, BD BioSciences) for 10 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Cells (1 x 106/mL) were then recovered and activated for 3 days on plate-bound anti-CD3 (BD, #553058) and soluble anti-CD28 (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... 1µm and 2µm) were added to 1 ml of PBS in 5mL round bottom polystyrene test tubes (BD Biosciences, UK) for flow cytometry and were run on a BD FACSVerse™ (BD Biosciences ...