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Citations for NanoTemper :
151 - 200 of 1068 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: MST measurements were performed using a NanoTemper Monolith NT.115 instrument (NanoTemper Technologies). Fluorescently labeled Codanin1Cterm Alexa Fluor 488 (250 nM ...
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bioRxiv - Molecular Biology 2023Quote: The capillaries were loaded into a Prometheus Panta instrument (NanoTemper Technologies). NanoDSF ...
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bioRxiv - Biophysics 2023Quote: ... as evaluated using the ThermControl software V2.1 (NanoTemper Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... Data were analysed by MO Control software provided by NanoTemper.
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bioRxiv - Microbiology 2023Quote: Protein thermostability was evaluated with a Prometheus NT.48 instrument (NanoTemper Technologies). Proteins at a concentration of 1 mg/mL were loaded to the grade capillaries and the intrinsic fluorescence signal was measured while temperature was increased by 1°C/min ...
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bioRxiv - Biochemistry 2023Quote: ... MST was measured using a Monolith NT.115 instrument (NanoTemper Technologies) at an ambient temperature of 20 °C ...
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bioRxiv - Biochemistry 2023Quote: ... and thermophoresis was measured on a Monolith NT.115 equipment (NanoTemper Technologies) with an IR laser power of 40% and an LED intensity of 60% ...
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bioRxiv - Biophysics 2023Quote: ... 0.2 mM ATP) with 0.05 % Tween-20 on a Monolith NT.115 Microscale Thermophoresis (MST) device using standard treated capillaries (NanoTemper Technologies). Actin (final labelled-actin concentrations adjusted to 60 nM ...
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bioRxiv - Biophysics 2023Quote: ... 115 premium capillaries were obtained from Nanotemper. NSP10 expression clones for mutants T12I ...
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bioRxiv - Biophysics 2023Quote: ... Measurements were done in triplicate by using the Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). The binding affinity tests for NSP14 ExoN and each NSP10 variant were repeated by the same procedure as carried out for native NSP10.
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ColG-variants were labeled using the Monolith NT™ Protein Labeling Kit RED-NHS 2nd Generation Amine reactive (NanoTemper, Germany) and the yield of labelled protein and the degree of labelling were determined according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2023Quote: ... Capillaries were filled by samples and loaded on the Prometheus NT48 using nano-DSF technology (Nanotemper). Temperature was increased 1°C/min from 20°C to 95°C and fluorescence at 330 nm (F330 ...
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bioRxiv - Biochemistry 2023Quote: Thermal denaturation of LceB was completed using a Prometheus NT.48 (NanoTemper). Purified protein in 50 mM Tris pH 7.5 250 mM NaCl was diluted 7-fold to a final concentration of 50 µM into Tris and HEPES buffer systems and 25-fold to a final concentration of 10 µM in Bis-Tris buffer systems ...
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bioRxiv - Biochemistry 2023Quote: ... 115 instrument (Nanotemper, München, Germany). The Nano-RED channel was used with 60 % excitation power and 60% MST power ...
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bioRxiv - Biochemistry 2023Quote: Thermal melts were determined using the Prometheus NT.48 (Nanotemper). Samples were loaded into Prometheus NT.Plex nanoDSF Grade High Sensitivity Capillary Chips and the laser intensity was set such that the discovery scan placed the auto fluorescence between the upper and lower bounds ...
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bioRxiv - Cancer Biology 2023Quote: Label-free thermal shift assay experiments were performed with a Tycho NT.6 instrument (NanoTemper Technologies) based on a reported protocol 76 ...
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bioRxiv - Cell Biology 2023Quote: IRE1αLD was labelled using the Monolith NT protein labelling Kit Red-Maleimide (NanoTemper). The concentration of the labelled IRE1αLD was kept constant at 10 nM ...
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bioRxiv - Cell Biology 2023Quote: His6-CTxA1 was labeled using Monolith NT His-Tag Labeling Kit RED-tris-NTA (NanoTemper). CTxB labeled with Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were loaded into Monolith NT.115 Premium Capillaries (NanoTemper Technologies) and incubated at 23 °C or 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The MST measurement was performed using the Monolith NT.115 Pico-red (NanoTemper Technologies) at 5% LED power and medium MST power ...
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bioRxiv - Bioengineering 2023Quote: ... Then 20 μL sample was loaded into standard treated capillaries for measurements on a Monolith NT Automated (Nanotemper).
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bioRxiv - Biophysics 2023Quote: ... A Prometheus NT.48 (NanoTemper Technologies GmbH, Munich, Germany) was used to assess Ub melting temperature ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... 6 (NanoTemper Technologies, Germany). The measurements were performed at 0.1 mg/ml protein concentration in 15 mM Tris-SO4 pH 7.5 ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: Microscale thermophoresis experiments were performed on a NanoTemper Monolith NT.115 instrument (NanoTemper Technologies). ColG variants were labeled using the Monolith NT™ Protein Labeling Kit RED-NHS 2nd Generation Amine reactive (NanoTemper Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... EED and JMJD3 proteins were labeled with Protein Labeling kit RED-NHS (NanoTemper, MO-L001). At least three independent experiments were performed for each binding reaction.
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: The dissociation constant (Kd) between DTX3L and USP28 was measured with Monolith (NanoTemper) using a RED-NHS 2ndgeneration labeling dye (NanoTemper) ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... using a RED-NHS 2ndgeneration labeling dye (NanoTemper). NHS ester groups covalently crosslink lysine residues ...
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bioRxiv - Biochemistry 2023Quote: 300 nM 6His-tagged EF-Tu was incubated with 75 nM Red Tris NTA dye Generation 2 (NanoTemper Technologies) in binding buffer (287 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: Microscale thermophoresis experiments were performed on a Monolith NT.115 system (NanoTemper Technologies) using YFP-fusion proteins.
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bioRxiv - Cancer Biology 2023Quote: ... inside standard capillaries (NanoTemper, MO-K002). EED and JMJD3 proteins were labeled with Protein Labeling kit RED-NHS (NanoTemper ...
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bioRxiv - Biochemistry 2023Quote: Data were collected using a Prometheus Panta nDSF instrument (Nanotemper). An initial panel of 20 nucleotides was chosen to screen for potential DUF ligands (Table S2) ...
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bioRxiv - Biochemistry 2023Quote: ... Data were processed using Pr.Analysis software (Nanotemper).
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bioRxiv - Neuroscience 2023Quote: ... Samples were then loaded into Monolith Premium Capillaries (NanoTemper Technologies GmbH). Measurements were done with Monolith NT.115 instrument (NanoTemper Technologies GmbH ...
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bioRxiv - Neuroscience 2023Quote: ... Kd values were calculated using the MO.Affinity Analysis software v2.3 (NanoTemper Technologies GmbH). Data are presented as ΔFnorm values ± SD from n = 4 independent experiments.
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bioRxiv - Neuroscience 2023Quote: ... Measurements were done with Monolith NT.115 instrument (NanoTemper Technologies GmbH) using red LED source ...
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bioRxiv - Neuroscience 2023Quote: ... was labelled via His-tag with Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies GmbH). The labelled complex was diluted in MST buffer (10 mM Na-phosphate buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... pH6) in a volume of 10 μl and placed in Prometheus Series High Sensitivity Capillaries (NanoTemper, Cat. no. PR-C006).
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bioRxiv - Molecular Biology 2023Quote: ... a volume of 10 μl was then placed in Prometheus Series High Sensitivity Capillaries (NanoTemper, Cat. no. PR-C006).
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bioRxiv - Microbiology 2023Quote: ... + 0.5% DDM and was labeled using a His-Tag Labeling Kit RED-tris-NTA 2nd Generation Kit (NanoTemper) via the manufacturers protocol in 1.7 ml low-adhesion tubes ...
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bioRxiv - Microbiology 2023Quote: ... Data were analyzed using MO Affinity Analysis v2.3 (NanoTemper Technologies GmbH, Germany). To prevent heat-induced artifacts and ...
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bioRxiv - Microbiology 2023Quote: ... measurements were performed using a Monolith Pico instrument (NanoTemper Technologies Gmbh, Munich, Germany) at an ambient temperature of 25 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The fluorescence intensity ratio and its first derivative were calculated with the manufacturer’s software (PR.ThermControl, version 2.3.1 from NanoTemper Technologies). For validating protein-mixture binding ...
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bioRxiv - Biophysics 2023Quote: His-tagged NSP16 was diluted to 400 nM in nsp16 buffer D and mixed with an equal volume of 100 nM RED-tris-NTA 2nd Generation labeling dye (NanoTemper Technologies GmbH, München, Germany). The mixture was incubated on ice for 1 h and centrifuged at 15,000 g ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Monitoring of the ITF at 330 nm and 350 nm during protein thermal denaturation was carried out in a Prometheus NT.48 instrument from NanoTemper Technologies (Munchen ...
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A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... were labeled with the amine-reactive dye NT-647 Red-NHS (NanoTemper, Munich, Germany) according to the manufacturer’s instructions ...
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A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... Affinity analysis v2.2.4 NT (NanoTemper).
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A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... protein quality was assessed using a Tycho NT.6 (NanoTemper, Munich, Germany). Labeled protein was dissolved in MST buffer (50mM Tris-HCl pH 7.8 ...
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bioRxiv - Systems Biology 2023Quote: ... NSP10 protein was fluorescently labeled using N-Hydroxysuccinimide (NHS)-ester fluorophores according to the manufacturer’s instructions (Protein Labeling Kit RED-NHS 2nd Generation, Nanotemper). Labeling was performed in the NSP16 protein storage buffer (20 mM HEPES pH 7.5 and 0.3 M NaCl) ...
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bioRxiv - Molecular Biology 2023Quote: ... NT.48 series nanoDSF grade standard capillaries (Nanotemper) were loaded with ∼25 μl of protein at 1-5 mg/ml in 20 mM sodium phosphate buffer ...
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bioRxiv - Systems Biology 2023Quote: ... using a Monolith MST device (Nanotemper) with 80% LED and medium infrared (IR ...