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Citations for NanoTemper :
1 - 50 of 1068 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: Premium treated capillaries (NanoTemper Technologies, for LCO and CO5-BODIPY) and standard capillaries (NanoTemper Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... and standard capillaries (NanoTemper Technologies, for CO) were loaded and the measurements were performed at 22°C with 60 % exitation power (CO5-BODIPY ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Protein and compound mixtures were loaded into standard treated capillaries (MO-K022, NanoTemper Technologies GmbH, Munich, Germany). Measurements were performed on a Monolith NT.115 with blue/red filters (NanoTemper Technologies GmbH ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Purified TRIM21 PRYSPRY domain was labeled using Protein Labeling Kit RED-NHS 2nd Generation (MO-L011, NanoTemper Technologies GmbH, Munich, Germany). Labeled protein was diluted to 50 nM in PBST (137 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Measurements were performed on a Monolith NT.115 with blue/red filters (NanoTemper Technologies GmbH, Munich, Germany) at 25 °C using 80% MST power ...
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bioRxiv - Biochemistry 2024Quote: The binding affinity of each recombinant TrK13 protein for heme was evaluated by MST analysis using the Monolith NT.115 instrument (NanoTemper Technologies). The recombinant TrK13 proteins (50 nM ...
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bioRxiv - Biochemistry 2024Quote: ... followed by labeling with cysteine-reactive dye (30 mM; Monolith Protein Labelling Kit Red-Maleimide 2nd Generation, NanoTemper) and incubated for 30 min at room temperature (RT ...
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bioRxiv - Biochemistry 2024Quote: ... the samples were loaded into capillary tubes for Tycho NT.6 instrument (NanoTemper). Tryptophan fluorescence at emission wavelengths of 350 nm and 330 nm were measured as the sample temperature was ramped from 35 °C to 95 °C according to the preprogrammed instrument protocol ...
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bioRxiv - Physiology 2024Quote: ... and then mounted in a Monolith NT.115 apparatus (Nanotemper Technologies) for binding measurements ...
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bioRxiv - Physiology 2024Quote: ... Data analysis was carried out with NTAffinityAnalysis software (NanoTemper Technologies).
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bioRxiv - Physiology 2024Quote: ... samples were loaded into NT.115 premium-treated capillaries (NanoTemper Technologies) and then mounted in a Monolith NT.115 apparatus (Nanotemper Technologies ...
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bioRxiv - Plant Biology 2024Quote: MST experiments were carried out using a Monolith NT.115 (NanoTemper Technologies GmbH). Purified insect cell-expressed RACK1A was fluorescently labeled with Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies GmbH MO-L011 ...
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bioRxiv - Plant Biology 2024Quote: ... Purified insect cell-expressed RACK1A was fluorescently labeled with Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies GmbH MO-L011) via amine conjugation ...
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bioRxiv - Biochemistry 2024Quote: ... Measurements were performed in triplets at 25 °C and at medium MST power in Monolith NT.115 Capillaries (NanoTemper Technologies). The raw data were analyzed using the MO.Affinity Analysis software (NanoTemper Technologies).
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bioRxiv - Biochemistry 2024Quote: ... coli was labeled using the Monolith Lys-Tag Labeling Kit RED-NHS second generation (NanoTemper Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Measurements were performed at 25 °C and at medium MST power in Monolith Premium Capillaries (NanoTemper Technologies). The raw data were analyzed using the MO.Affinity Analysis software (NanoTemper Technologies).
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bioRxiv - Biochemistry 2024Quote: Spectral shift assay was performed on a Monolith X instrument (NanoTemper Technologies) in parallel with MST measurements ...
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bioRxiv - Biochemistry 2024Quote: Thermal shift assays were carried out in triplicate on the Prometheus NT.48 nanoDSF instrument (NanoTemper Technologies) using the manufacturer-designated capillaries ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 10 ul was sampled in a Monolith.NT115 capillary (NanoTemper MO-K022) and measurements were performed at 25°C using standard parameters ...
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bioRxiv - Biochemistry 2024Quote: DSF assays were performed using Tycho NT.6 (NanoTemper Technologies). RBDs were diluted to approximatively 1 mg/mL ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we mixed different ratios of Fcy1Labeled and Fcy1Unlabeled following the AssayControl software (NanoTemper, Germany) suggested protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used the Monolith Protein Labeling Kit RED-NHS 2nd Generation kit (NanoTemper MO- L011) to label ∼1 lysine per Fcy1 monomer with a fluorophore following the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we measured emission spectral shift of covalently fluorescent labeled Fcy1WT using a Monolith X platform (NanoTemper, Germany). We used the Monolith Protein Labeling Kit RED-NHS 2nd Generation kit (NanoTemper MO- L011 ...
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bioRxiv - Evolutionary Biology 2024Quote: We measured protein stability using nano-differential scanning fluorimetry on a Prometheus NT.48 (NanoTemper). After purification and his-tag cleavage ...
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bioRxiv - Biophysics 2024Quote: ... Experiments were conducted in duplicate on a Monolith NT.115 system (NanoTemper Technologies). To estimate the effect of metal ions ...
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bioRxiv - Biochemistry 2024Quote: The MST experiments were conducted using a Monolith NT.115 instrument (NanoTemper Technologies). Purified SIDT1FL and PNGase F-treated SIDT1FL were exchanged into a labeling buffer containing 25 mM NaHCO3 pH 8.3 ...
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bioRxiv - Genetics 2024Quote: ... The samples were added to monolith capillaries (NanoTemper, MO-L022) and subsequently subjected to MST analysis ...
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bioRxiv - Genetics 2024Quote: The MST experiments were conducted on a Monolith NT.115 system (NanoTemper Technologies, Germany). Lysates of the HEK293T cells transfected with fluorescent GFP-IQCH or GFP-IQCH(△IQ ...
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bioRxiv - Microbiology 2024Quote: ... using the Tycho NT.6 (NanoTemper). DSF experiments were set up to a final volume of 10 µl of buffer C containing the EccC5DUF construct at 2 µM in the absence or in the presence of ADP-AlF3 at 4 mM ...
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bioRxiv - Microbiology 2024Quote: ... and the NHS-red-tag kit (Nanotemper, Germany) was used to label ConA (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... and analyzed by NanoTemper Monilith Instrument (NT.115) (NanoTemper Technologies) with 20% LED power and 20% MST power ...
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bioRxiv - Molecular Biology 2024Quote: ... and analyzed by NanoTemper Monilith Instrument (NT.115 ...
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bioRxiv - Neuroscience 2024Quote: We followed the manufacturer’s instructions to label APOE3 and APOE4 using the Monolith protein labeling kit RED-NHS 2nd generation (NanoTemper Technologies). In summary ...
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bioRxiv - Physiology 2024Quote: ... cMyBP-C constructs were fluorescently labeled via His-tag using a commercially available kit (NanoTemper Technologies). Unlabeled sS1 or 15-hep myosin was titrated against 84 nM fluorescently labeled C3 or C0C7 protein ...
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bioRxiv - Molecular Biology 2024Quote: ... the mixtures were loaded into capillaries (NanoTemper Technologies) and analyzed by NanoTemper Monilith Instrument (NT.115 ...
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bioRxiv - Molecular Biology 2024Quote: ... and the buffers were replaced by PBS buffer with 0.005% Tween-20 (PBST) using column A (NanoTemper Technologies). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... The Kd values and Hill coefficients (nH) were calculated using MO Affinity Analysis software (NanoTemper). All data were statistically analyzed and graphed using GraphPad Prism software (v5.0).
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bioRxiv - Molecular Biology 2024Quote: MicroScale Thermophoresis (MST) assay was performed on a Monolith NT.115 instrument (NanoTemper Technologies GmbH) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Affinity Analysis 3 Software (Nanotemper) using a Kd fit model and constraining the target concentration.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Data analysis was performed using MO.Affinity Analysis v2.3 software (NanoTemper Technologies), with data fit to a Kd model.
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bioRxiv - Pharmacology and Toxicology 2024Quote: Microscale thermophoresis (MST) experiments were performed using the Monolith NT.Automated instrument (NanoTemper Technologies). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 30 min with 50 nM RED-tris-NTA 2nd Generation dye from the Monolith His-Tag Labeling Kit (Cat.#MO-L018; NanoTemper Technologies). The labeling reaction mixture was centrifuged for 10 min at 4°C and 15,000 x g to remove protein aggregates ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were soaked into standard capillaries (NanoTemper Technologies). Measurements were performed at 25°C ...
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bioRxiv - Molecular Biology 2024Quote: Binding affinity comparisons via microscale thermophoresis were performed using the Monolith NT.115 instrument (NanoTemper Technologies). The CTD polypeptides (CTD ...
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bioRxiv - Cell Biology 2024Quote: ... DECR1 experiments were performed using Premium Capillaries (Nanotemper, MO-K025) Analysis was performed using the MO Affinity software provided by the manufacturer.
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bioRxiv - Biochemistry 2024Quote: ... using a Prometheus NT.48 (NanoTemper Technologies). For sample preparation ...
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bioRxiv - Biochemistry 2024Quote: ... The measured curves were analysed using the manufacturer’s software (PR.ThermControl, v 2.1.2, NanoTemper Technologies). Based on the determined Tm values ...
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bioRxiv - Biochemistry 2024Quote: ... The raw data were analyzed using the MO.Affinity Analysis software (NanoTemper Technologies).
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bioRxiv - Biochemistry 2024Quote: ... Then all samples were loaded into Prometheus NT.48 nanoDSF Grade Standard Capillaries (PR-C002, NanoTemper Technologies), the fluorescence signal was recorded under a temperature gradient ranging from 25 to 90◦C (flow rate of 0.5◦C·min−1) ...
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bioRxiv - Biochemistry 2024Quote: ... were carried out using the nanoDSF method as implemented in the Prometheus NT.48 (NanoTemper Technologies). The nanoDSF method is based on the autofluorescence of tryptophan (and tyrosine ...