Labshake search

1 - 50 of 1200 citations since 2020

• Broad therapeutic benefit of myosin inhibition in hypertrophic cardiomyopathy

  Laura Sen-Martín, et al., bioRxiv - Physiology 2024

  Quote: ... cMyBP-C constructs were fluorescently labeled via His-tag using a commercially available kit (NanoTemper Technologies). Unlabeled sS1 or 15-hep myosin was titrated against 84 nM fluorescently labeled C3 or C0C7 protein ...
• Broad therapeutic benefit of myosin inhibition in hypertrophic cardiomyopathy

  Laura Sen-Martín, et al., bioRxiv - Physiology 2024

  Quote: ... and then mounted in a Monolith NT.115 apparatus (Nanotemper Technologies) for binding measurements ...
• Broad therapeutic benefit of myosin inhibition in hypertrophic cardiomyopathy

  Laura Sen-Martín, et al., bioRxiv - Physiology 2024

  Quote: ... Data analysis was carried out with NTAffinityAnalysis software (NanoTemper Technologies).
• Broad therapeutic benefit of myosin inhibition in hypertrophic cardiomyopathy

  Laura Sen-Martín, et al., bioRxiv - Physiology 2024

  Quote: ... samples were loaded into NT.115 premium-treated capillaries (NanoTemper Technologies) and then mounted in a Monolith NT.115 apparatus (Nanotemper Technologies ...
• Identification of RACK1A as a component of the auxin-ethylene crosstalk regulating apical hook development in Arabidopsis thaliana

  Qian Ma, et al., bioRxiv - Plant Biology 2024

  Quote: MST experiments were carried out using a Monolith NT.115 (NanoTemper Technologies GmbH). Purified insect cell-expressed RACK1A was fluorescently labeled with Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies GmbH MO-L011 ...
• Identification of RACK1A as a component of the auxin-ethylene crosstalk regulating apical hook development in Arabidopsis thaliana

  Qian Ma, et al., bioRxiv - Plant Biology 2024

  Quote: ... Purified insect cell-expressed RACK1A was fluorescently labeled with Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies GmbH MO-L011) via amine conjugation ...
• Development of quantitative high-throughput screening assays to identify, validate, and optimize small-molecule stabilizers of misfolded β-glucocerebrosidase with therapeutic potential for Gaucher disease and Parkinson’s disease

  Darian Williams, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: ... Data analysis was performed using MO.Affinity Analysis v2.3 software (NanoTemper Technologies), with data fit to a Kd model.
• Development of quantitative high-throughput screening assays to identify, validate, and optimize small-molecule stabilizers of misfolded β-glucocerebrosidase with therapeutic potential for Gaucher disease and Parkinson’s disease

  Darian Williams, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: Microscale thermophoresis (MST) experiments were performed using the Monolith NT.Automated instrument (NanoTemper Technologies). Briefly ...
• Development of quantitative high-throughput screening assays to identify, validate, and optimize small-molecule stabilizers of misfolded β-glucocerebrosidase with therapeutic potential for Gaucher disease and Parkinson’s disease

  Darian Williams, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: ... for 30 min with 50 nM RED-tris-NTA 2nd Generation dye from the Monolith His-Tag Labeling Kit (Cat.#MO-L018; NanoTemper Technologies). The labeling reaction mixture was centrifuged for 10 min at 4°C and 15,000 x g to remove protein aggregates ...
• Characterization of N-glycosylation and its Functional Role in SIDT1-Mediated RNA Uptake

  Tingting Yang, et al., bioRxiv - Biochemistry 2024

  Quote: The MST experiments were conducted using a Monolith NT.115 instrument (NanoTemper Technologies). Purified SIDT1FL and PNGase F-treated SIDT1FL were exchanged into a labeling buffer containing 25 mM NaHCO3 pH 8.3 ...
• New insights into the domain of unknown function DUF of EccC₅, the pivotal ATPase providing the secretion driving force to the ESX5 secretion system

  Fernando Ceballos-Zúñiga, et al., bioRxiv - Microbiology 2024

  Quote: ... using the Tycho NT.6 (NanoTemper). DSF experiments were set up to a final volume of 10 µl of buffer C containing the EccC5DUF construct at 2 µM in the absence or in the presence of ADP-AlF3 at 4 mM ...
• SARS-CoV-2 methyltransferase nsp10-16 in complex with natural and drug-like purine analogs for guiding structure-based drug discovery

  Viviane Kremling, et al., bioRxiv - Biochemistry 2024

  Quote: ... Measurements were performed in triplets at 25 °C and at medium MST power in Monolith NT.115 Capillaries (NanoTemper Technologies). The raw data were analyzed using the MO.Affinity Analysis software (NanoTemper Technologies).
• SARS-CoV-2 methyltransferase nsp10-16 in complex with natural and drug-like purine analogs for guiding structure-based drug discovery

  Viviane Kremling, et al., bioRxiv - Biochemistry 2024

  Quote: ... coli was labeled using the Monolith Lys-Tag Labeling Kit RED-NHS second generation (NanoTemper Technologies), according to the manufacturer’s instructions ...
• SARS-CoV-2 methyltransferase nsp10-16 in complex with natural and drug-like purine analogs for guiding structure-based drug discovery

  Viviane Kremling, et al., bioRxiv - Biochemistry 2024

  Quote: ... Measurements were performed at 25 °C and at medium MST power in Monolith Premium Capillaries (NanoTemper Technologies). The raw data were analyzed using the MO.Affinity Analysis software (NanoTemper Technologies).
• SARS-CoV-2 methyltransferase nsp10-16 in complex with natural and drug-like purine analogs for guiding structure-based drug discovery

  Viviane Kremling, et al., bioRxiv - Biochemistry 2024

  Quote: Spectral shift assay was performed on a Monolith X instrument (NanoTemper Technologies) in parallel with MST measurements ...
• SARS-CoV-2 methyltransferase nsp10-16 in complex with natural and drug-like purine analogs for guiding structure-based drug discovery

  Viviane Kremling, et al., bioRxiv - Biochemistry 2024

  Quote: Thermal shift assays were carried out in triplicate on the Prometheus NT.48 nanoDSF instrument (NanoTemper Technologies) using the manufacturer-designated capillaries ...
• A new group of LysM-RLKs involved in symbiotic signal perception and arbuscular mycorrhiza establishment

  Yi Ding, et al., bioRxiv - Plant Biology 2024

  Quote: Premium treated capillaries (NanoTemper Technologies, for LCO and CO5-BODIPY) and standard capillaries (NanoTemper Technologies ...
• A new group of LysM-RLKs involved in symbiotic signal perception and arbuscular mycorrhiza establishment

  Yi Ding, et al., bioRxiv - Plant Biology 2024

  Quote: ... and standard capillaries (NanoTemper Technologies, for CO) were loaded and the measurements were performed at 22°C with 60 % exitation power (CO5-BODIPY ...
• Selective degradation of multimeric proteins via chemically induced proximity to TRIM21

  Panrui Lu, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: ... Protein and compound mixtures were loaded into standard treated capillaries (MO-K022, NanoTemper Technologies GmbH, Munich, Germany). Measurements were performed on a Monolith NT.115 with blue/red filters (NanoTemper Technologies GmbH ...
• Selective degradation of multimeric proteins via chemically induced proximity to TRIM21

  Panrui Lu, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: Purified TRIM21 PRYSPRY domain was labeled using Protein Labeling Kit RED-NHS 2nd Generation (MO-L011, NanoTemper Technologies GmbH, Munich, Germany). Labeled protein was diluted to 50 nM in PBST (137 mM NaCl ...
• Selective degradation of multimeric proteins via chemically induced proximity to TRIM21

  Panrui Lu, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: ... Measurements were performed on a Monolith NT.115 with blue/red filters (NanoTemper Technologies GmbH, Munich, Germany) at 25 °C using 80% MST power ...
• Ca²⁺-independent ZmCPK2 is inhibited by Ca²⁺-dependent ZmCPK17 during drought response in maize

  Xiaoying Hu, et al., bioRxiv - Molecular Biology 2024

  Quote: ... the mixtures were loaded into capillaries (NanoTemper Technologies) and analyzed by NanoTemper Monilith Instrument (NT.115 ...
• Ca²⁺-independent ZmCPK2 is inhibited by Ca²⁺-dependent ZmCPK17 during drought response in maize

  Xiaoying Hu, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and the buffers were replaced by PBS buffer with 0.005% Tween-20 (PBST) using column A (NanoTemper Technologies). Next ...
• Biochemical characterization of the Escherichia coli surfaceome: A focus on type I fimbriae and flagella

  DW Kavanaugh, et al., bioRxiv - Microbiology 2024

  Quote: ... and the NHS-red-tag kit (Nanotemper, Germany) was used to label ConA (Sigma ...
• High-Throughput Identification of Calcium Regulated Proteins Across Diverse Proteomes

  Timothy M. Locke, et al., bioRxiv - Cell Biology 2024

  Quote: ... DECR1 experiments were performed using Premium Capillaries (Nanotemper, MO-K025) Analysis was performed using the MO Affinity software provided by the manufacturer.
• Tetrameric INTS6-SOSS1 complex facilitates DNA:RNA hybrid autoregulation at double-strand breaks

  Qilin Long, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Samples were soaked into standard capillaries (NanoTemper Technologies). Measurements were performed at 25°C ...
• Tetrameric INTS6-SOSS1 complex facilitates DNA:RNA hybrid autoregulation at double-strand breaks

  Qilin Long, et al., bioRxiv - Molecular Biology 2024

  Quote: Binding affinity comparisons via microscale thermophoresis were performed using the Monolith NT.115 instrument (NanoTemper Technologies). The CTD polypeptides (CTD ...
• Ca²⁺-independent ZmCPK2 is inhibited by Ca²⁺-dependent ZmCPK17 during drought response in maize

  Xiaoying Hu, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and analyzed by NanoTemper Monilith Instrument (NT.115) (NanoTemper Technologies) with 20% LED power and 20% MST power ...
• Ca²⁺-independent ZmCPK2 is inhibited by Ca²⁺-dependent ZmCPK17 during drought response in maize

  Xiaoying Hu, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and analyzed by NanoTemper Monilith Instrument (NT.115 ...
• APOE regulates the transport of GM1

  Dong Yan Zhang, et al., bioRxiv - Neuroscience 2024

  Quote: ... The 16 capillary tubes (NanoTemper Technologies) were inserted into each sample tube to enable sample entry into the capillary ...
• APOE regulates the transport of GM1

  Dong Yan Zhang, et al., bioRxiv - Neuroscience 2024

  Quote: We followed the manufacturer’s instructions to label APOE3 and APOE4 using the Monolith protein labeling kit RED-NHS 2nd generation (NanoTemper Technologies). In summary ...
• Mitochondrial Raf1 Regulates Glutamine Catabolism

  Ronald L. Shanderson, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Samples placed in Monolith NT.115 capillaries or NT.115 premium capillaries (MO-K022, MO-K-025, Nanotemper Technologies) and measured on Monolith NT.115 instrument (Nanotemper Technologies) ...
• C1q/MASP complexes – hybrid complexes of classical and lectin pathway proteins are found in the circulation

  Anne Rosbjerg, et al., bioRxiv - Immunology 2024

  Quote: ... Affinity Analysis 3 Software (Nanotemper) using a Kd fit model and constraining the target concentration.
• The interplay between peptides and RNA is critical for protoribosome compartmentalization and stability

  Simone Codispoti, et al., bioRxiv - Biophysics 2024

  Quote: ... Experiments were conducted in duplicate on a Monolith NT.115 system (NanoTemper Technologies). To estimate the effect of metal ions ...
• Compensatory mutations potentiate constructive neutral evolution by gene duplication

  Philippe C Després, et al., bioRxiv - Evolutionary Biology 2024

  Quote: ... 10 ul was sampled in a Monolith.NT115 capillary (NanoTemper MO-K022) and measurements were performed at 25°C using standard parameters ...
• SARS-CoV-2 Omicron XBB lineage spike structures, conformations, antigenicity, and receptor recognition

  Qianyi E. Zhang, et al., bioRxiv - Biochemistry 2024

  Quote: DSF assays were performed using Tycho NT.6 (NanoTemper Technologies). RBDs were diluted to approximatively 1 mg/mL ...
• Compensatory mutations potentiate constructive neutral evolution by gene duplication

  Philippe C Després, et al., bioRxiv - Evolutionary Biology 2024

  Quote: ... we mixed different ratios of Fcy1Labeled and Fcy1Unlabeled following the AssayControl software (NanoTemper, Germany) suggested protocol ...
• Compensatory mutations potentiate constructive neutral evolution by gene duplication

  Philippe C Després, et al., bioRxiv - Evolutionary Biology 2024

  Quote: ... We used the Monolith Protein Labeling Kit RED-NHS 2nd Generation kit (NanoTemper MO- L011) to label ∼1 lysine per Fcy1 monomer with a fluorophore following the manufacturer’s instruction ...
• Compensatory mutations potentiate constructive neutral evolution by gene duplication

  Philippe C Després, et al., bioRxiv - Evolutionary Biology 2024

  Quote: ... we measured emission spectral shift of covalently fluorescent labeled Fcy1WT using a Monolith X platform (NanoTemper, Germany). We used the Monolith Protein Labeling Kit RED-NHS 2nd Generation kit (NanoTemper MO- L011 ...
• Compensatory mutations potentiate constructive neutral evolution by gene duplication

  Philippe C Després, et al., bioRxiv - Evolutionary Biology 2024

  Quote: We measured protein stability using nano-differential scanning fluorimetry on a Prometheus NT.48 (NanoTemper). After purification and his-tag cleavage ...
• Caskin2 is a novel talin and Abi1-binding protein that promotes cell motility

  Wei Wang, et al., bioRxiv - Cell Biology 2024

  Quote: MST measurements were carried out using premium-coated capillaries to reduce non-specific interaction of the proteins with the glass surface on a Monolith NT.115 red-blue (Nanotemper, Munich, Germany), as described previously (Zuidema et al. ...
• Caskin2 is a novel talin and Abi1-binding protein that promotes cell motility

  Wei Wang, et al., bioRxiv - Cell Biology 2024

  Quote: ... and finally analyzed using the MO Affinity Analysis Software (Nanotemper).
• Effect of leaflet asymmetry on the stretching elasticity of lipid bilayers with phosphatidic acid

  Dominik Drabik, et al., bioRxiv - Biophysics 2024

  Quote: ... Protein Labeling Kit RED-NHS 2nd Generation was purchased from NanoTemper Technologies (München ...
• Apolipoprotein A1 and high-density lipoprotein limit low-density lipoprotein transcytosis by binding scavenger receptor B1

  Karen Y. Y. Fung, et al., bioRxiv - Biochemistry 2024

  Quote: ... and diluted in MST buffer (NanoTemper Technologies) to a concentration of 50 nM ...
• Apolipoprotein A1 and high-density lipoprotein limit low-density lipoprotein transcytosis by binding scavenger receptor B1

  Karen Y. Y. Fung, et al., bioRxiv - Biochemistry 2024

  Quote: ... Fluorescence was determined in a thermal gradient at 40% LED power and high MST power generated by a Monolith NT.115 from NanoTemper Technologies ...
• Apolipoprotein A1 and high-density lipoprotein limit low-density lipoprotein transcytosis by binding scavenger receptor B1

  Karen Y. Y. Fung, et al., bioRxiv - Biochemistry 2024

  Quote: ... was labeled using the Monolith His-Tag Labeling Kit Red-tris-NTA (NanoTemper Technologies) and diluted in MST buffer (NanoTemper Technologies ...
• Apolipoprotein A1 and high-density lipoprotein limit low-density lipoprotein transcytosis by binding scavenger receptor B1

  Karen Y. Y. Fung, et al., bioRxiv - Biochemistry 2024

  Quote: MST experiments were performed on a Monolith NT.115 instrument (NanoTemper Technologies). Purified His-tagged SR-B1 protein (His6.hSR-B1(amino acids 33 - 444) ...
• Activity modulation in anaerobic ribonucleotide reductases: nucleotide binding to the ATP-cone allosterically mediates substrate binding to the active site

  Ornella Bimaï, et al., bioRxiv - Biochemistry 2024

  Quote: ... Affinity Analysis v2.3 software (NanoTemper Technologies) with default parameters ...
• Activity modulation in anaerobic ribonucleotide reductases: nucleotide binding to the ATP-cone allosterically mediates substrate binding to the active site

  Ornella Bimaï, et al., bioRxiv - Biochemistry 2024

  Quote: ... MST was performed using the Monolith NT.115 instrument (NanoTemper Technologies GmbH) at room temperature ...
• Activity modulation in anaerobic ribonucleotide reductases: nucleotide binding to the ATP-cone allosterically mediates substrate binding to the active site

  Ornella Bimaï, et al., bioRxiv - Biochemistry 2024

  Quote: ... The samples were transferred to Monolith™ NT.115 Series Premium Capillaries (NanoTemper Technologies GmbH), which were scanned using the MST instrument (100% excitation power ...