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Citations for PolyScience :
251 - 284 of 284 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Transfections were performed with 800 ng of single or 1600 ng of mixed plasmid DNA complexed to polyethylenimine (PEI) (Polyscience Inc., PA, USA) in serum-free DMEM ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected with 800 ng of mHilpda_mCherry plasmid complexed to polyethylenimine (PEI) (Polyscience Inc., PA, USA) in serum-free DMEM ...
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bioRxiv - Biochemistry 2020Quote: ... Next day cells were transfected with 750 ng of Hilpda_sYFP2 complexed to polyethylenimine (PEI) (Polyscience Inc., PA, USA) in serum free medium ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were expressed by transient co-transfection of both heavy and light chain plasmids in Expi293F cells using PEI MAX (Polyscience) transfection reagent ...
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bioRxiv - Immunology 2020Quote: ... The cultures were transfected using PEI-MAX (Polyscience) with cells grown to a density of 3.0 million cells per mL and cultivated for 3 days ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then transfected with 1μg sgRNA plasmid by using 1 mg/ml Polyethyleneimine MAX (Polyscience). Puromycin was used to selecte the transfected cells ...
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bioRxiv - Neuroscience 2020Quote: ... Transfection of HEK293T cells was carried out using 1 mg/ml Polyethyleneimine MAX (Polyscience). Mycoplasma contamination was monitored by DAPI staining.
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bioRxiv - Microbiology 2020Quote: HEK293T cells were transfected using the PEImax (Cat# 24765-2, MW 40,000, Polyscience, Inc.) at a ratio of 1:3 of DNA ...
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bioRxiv - Cancer Biology 2020Quote: ... PEI MW25K (Polyscience Inc., Warrington, Pa, USA) was dissolved in water to final stock concentration of 1 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... infected cells were en bloc stained with uranyl acetate and lead aspartate then samples were dehydrated in acetone solutions of increasing concentrations (30-100%) and embedded in PolyBed 812 resin (Polyscience Inc., Warrington, PA, USA) using flat-embedding molds (EMS ...
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bioRxiv - Biophysics 2020Quote: ... carboxylate polystyrene beads (0.52 µm, Polyscience #09836-15) were coated with an Anti-Strep-tagII antibody (Abcam #ab76949 ...
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bioRxiv - Biophysics 2020Quote: The accuracy of positional measurements was verified with beads (300-nm latex beads; Polyscience, Niles, IL, USA) of similar size compared to the synaptic cargos ...
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bioRxiv - Cell Biology 2020Quote: ... ~2x 107 3-μm latex NH2-beads (Polyscience, Eppelheim, Germany) were activated with 8% glutaraldehyde for four h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells were seeded into 10 cm culture dishes and transfected the next day using polyethyleneimine (PolyScience, Niles, IL) with the following plasmids ...
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bioRxiv - Neuroscience 2020Quote: ... Infiltrated cochleae were then incubated in pure resin overnight before being placed in embedding molds (Polyscience, Germany) and incubated in a pre-warmed oven (70 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 0.15 μL of PEI-Max (Polyscience), 20 ng of sensor vector or control sensor (− ...
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bioRxiv - Microbiology 2020Quote: A pME18S expression plasmid containing the full-length or subdomain spike protein was transiently transfected into HEK293T cells using PEI max (PolyScience); the pMx-GFP expression plasmids were used as the marker of transfected cells ...
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bioRxiv - Neuroscience 2020Quote: ... were cut on the ultramicrotome with a diamond knife (Jumdi, 2 mm) and then collected onto TEM copper grids (Gilder) and stained with 2% uranyl acetate (Polyscience) and 1% lead citrate (Reynolds 1963) ...
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bioRxiv - Bioengineering 2020Quote: ... 90 µm Polybead Microspheres (Polyscience, Inc.) were placed between the slide and the coverslip (No ...
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bioRxiv - Synthetic Biology 2020Quote: ... by using polyethyleneimine “Max” MW 40,000 (Polyscience, Warrington, PA). Virus-containing media were collected 48 hours after transfection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... or PEI Max: Polyethyleneimine “Max” (MW 40,000) (PolyScience, Inc.).
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bioRxiv - Immunology 2020Quote: ... using polyethylenimine (PEI; Polyscience) into HEK293T cells for virus production ...
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bioRxiv - Microbiology 2020Quote: ... Individual plasmids or a combination of the gH and gL expression plasmids (4:1 ratio) were transfected into HEK293T cells using polyethylenimine (PolyScience) as described previously (33) ...
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bioRxiv - Cancer Biology 2020Quote: ... Retroviral particles were produced in HEK293T cells after co-transfection of retrovirus plasmid vector pWZL-Blast-Flag-Snail or control vector pWZL-Blast-Flag-Empty with packaging plasmids (VSVG, Gag/pol) using polyethylenimine (PEI) (Polyscience). After 48h and 72h ...
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bioRxiv - Physiology 2020Quote: ... Polystyrene black dyed microspheres (diameter 6 μm, 2.6% solid-latex, Polybead, Polyscience Inc., Warrington, PA) were washed and diluted with physiological solution (0,5% latex ...
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bioRxiv - Immunology 2020Quote: ... The expression vectors were transient transfected into HEK-293F cell lines (Clover Biopharma) using PEI (Polyscience) and grown in OPM-293 CD05 medium (OPM ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were transfected with pAAV/GFP or pAAV/sCSF1R-GFP, pHelper, and pAAV9-RC (Gene Therapy Program, University of Pennsylvania) using linear polyethylenimine (PEI, MW. 25,000, Polyscience) with a PEI:DNA ratio of 4:1 in weight ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were fixed on microscopic slides with Aqua-Poly/Mount (Polyscience Inc.). Images were acquired with a Leica SP5 confocal microscope using 20x and 40x objectives (N.A ...
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bioRxiv - Cell Biology 2020Quote: Cells grown on coverslips were fixed with 4% formaldehyde (Polyscience, 18814) for 20⍰min in room temperature and permeabilized with 0.05% Saponin (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: To stain for myelin with luxol fast blue (Polyscience, Hirschberg an der Bergstraße) free floating sections were washed two times with 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... 293T cells were cotransfected with pcDNA3.1-Env clone and Env-deficient HIV-1 backbone vector (pSG3△Env) using PEI transfection reagent (PolyScience). Pseudovirus-containing supernatant was harvested 48h post-transfection ...
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bioRxiv - Immunology 2020Quote: ... pAdVAntage using polyethylenimine (PEI; Polyscience) for virus production ...
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bioRxiv - Neuroscience 2020Quote: ... and then transcardially perfused with physiological saline followed by an ice-cold 0.1M phosphate-buffered (pH 7.4) fixative containing 4% PFA and 0.1% glutaraldehyde (Polyscience Europe GmbH, Eppelheim, Germany) for 15min ...
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bioRxiv - Cancer Biology 2020Quote: ... Hematoxylin phloxine saffron stain (HPS) and Masson’s Trichome stain (cat. #25088; Polyscience, Warrington, PA) were performed on 5 µm-thick paraffin sections ...