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Citations for PolyScience :
101 - 150 of 284 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... For the retrograde tracing of lung-innervating VG neurons mice received a volume of 40 µl Fast Blue solution (350 µM and 1% DMSO in 0.9% saline, 73819-41-7, Polyscience Inc) or 40 µl DiD solution (0.5 m/ml in 0.9% saline ...
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bioRxiv - Neuroscience 2023Quote: ... 1-2 μL PCR product mixed with 0.9 μL PEI MAX (Polyscience, 24765-100) was directly transfected into HEK293T cells in the 96-well plate ...
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bioRxiv - Immunology 2023Quote: ... by co-transfection of heavy and light chain expressing plasmids using polyethylenimine (Polyscience) and cells were cultured at 37 °C with shaking at 125 rpm and 8% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... 300 µL of 1 mg/mL polyethylenimine linear 25k (Polyscience, 23966) was diluted into 5 mL OptiMEM in a separate tube ...
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bioRxiv - Immunology 2023Quote: ... using Polyethylenimine (PolyScience 23966-100) or FuGENE (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... In a separate tube 12 μL of PEI (Polyscience 24765), formulated at 1 mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... The coil was jacketed and connected to a water chiller (Durachill©, PolyScience) to remove heat and thereby control the coil temperature (Fig 1A) ...
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bioRxiv - Cell Biology 2023Quote: ... transfection was performed using PEI (Polyscience) with ΔCnA and CnB plasmids and the corresponding empty vectors ...
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bioRxiv - Cell Biology 2023Quote: ... Gag-pol packaging vectors and the retroviral vector (empty or containing Parkin-flag) by PEI (Polyscience) direct transfection ...
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bioRxiv - Cell Biology 2023Quote: ... Polybrene (Polyscience) was added to a final concentration of 6 μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The bead stock solution (Fluoresbrite YG microsphere, Cat. 17154-10, Polyscience) with a concentration of 4.55 × 1010 particles mL-1 ...
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bioRxiv - Developmental Biology 2023Quote: ... The minigene constructs (c.21-94G>A or WT) were transfected into cultured 293T cells using PEI reagent (Polyscience). Cells were harvested 48 h after transfection and total RNA was extracted using TRIzol following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Transient transfections were performed using PEI reagent (Polyscience). Cells were collected 48 hours post transfection.
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded onto assay plates and transfected using polyethyleneimine (PEI, Polyscience, 1 mg/mL). Details on the conditions for each experiment can be found in Supplementary Table 2 ...
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bioRxiv - Systems Biology 2023Quote: ... 4.5 μm fluorescent beads (100 beads/μL, YG-beads, Polyscience) were added to the sample as an internal particle concentration standard ...
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bioRxiv - Immunology 2023Quote: ... and all plasmid constructs used throughout the study were transfected into cells using PEI transfection reagent (Polyscience, Cat; 24765-1) at a concentration of 1□µg/ml ...
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bioRxiv - Plant Biology 2023Quote: ... with uranyl acetate (Polyscience Inc. Eppelheim, Germany) for 30 minutes followed by incubation in Reynolds’ lead citrate (SERVA ...
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bioRxiv - Bioengineering 2023Quote: ... or at 37 °C in a water bath (PolyScience), and analyzed by dynamic light scattering or protein thermal shift assay ...
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bioRxiv - Biophysics 2023Quote: ... a polyethylenimine (PEI) based method (Polyscience) was used ...
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bioRxiv - Genetics 2023Quote: ... and polyethylenimine (PEI, Polyscience) was added in a 1:3 ratio (1 mg of DNA to 3 mg of PEI) ...
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bioRxiv - Biophysics 2023Quote: ... Probe particles (Polybead® Microspheres 1 μm, Polyscience, Inc) were suspended in 60% Glycerol (ROTIPURAN® ...
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bioRxiv - Biophysics 2023Quote: ... Previously 1 μm beads (Polybead® Microspheres 1 μm, Polyscience, Inc) were added in 1:10,000 dilution to the medium ...
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bioRxiv - Molecular Biology 2023Quote: ... Rev and Gag-pol using PEI reagent (PEI MAX, Polyscience, 24765-2). The supernatant was collected after 48 and 72 h ...
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bioRxiv - Developmental Biology 2023Quote: ... 2-4 cell stage embryos were mounted between two cover slips in M9 buffer containing 20 μm microspheres (Polyscience). Dual-color 3D time-lapse imaging was performed using a Spinning disc confocal microscopy ...
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bioRxiv - Bioengineering 2023Quote: ... One (1) μm yellow PSP and 6 μm red PSP were from Polyscience Inc ...
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bioRxiv - Immunology 2023Quote: ... HEK293T cells were transfection with the indicated spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
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bioRxiv - Cell Biology 2023Quote: ... with PEI (Polyscience, 23966) for 48 hours according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were co-transfected using PEI-MAX (1μg/ml; Polyscience, 324765) with VSV-G (Addgene#8454) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were mounted using Aqua Polymount (Polyscience). Images were acquired using a Leica SP5 confocal microscope (10× 0.4NA or 40× 1.25NA objective ...
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bioRxiv - Cell Biology 2023Quote: ... and pCMV-VSV-G-RSV-Rev (RIKEN BioResource Center) were co-transfected into Lenti-X 293T cells using polyethyleneimine (Polyscience). Virus-containing media were collected at 48 or 72 h after transfection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The saturated aerosol then entered the condenser (Model AP15R-40-A11B, PolyScience Inc., Niles, IL, USA), where it was rapidly cooled to about −7°C ...
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bioRxiv - Neuroscience 2023Quote: ... blue fluorescent neuronal tracer fast blue (Polyscience Inc, 17740-1, 1mg) onto the surface of L1 for 5 minutes ...
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bioRxiv - Genomics 2023Quote: ... Polyethylenimine (PEI) (Polyscience 23966-2) was added at a 3:1 PEI:DNA ratio and the mixture briefly vortexed and incubated at RT for 10-20 mins ...
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bioRxiv - Cell Biology 2023Quote: ... using PEI MAX transfection reagent (Polyscience, 24765) for 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 µg lentiviral plasmid using PEI-Max (Polyscience, cat.no. 24765-1). Lentiviral supernatant was harvested 36 ...
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bioRxiv - Cell Biology 2023Quote: ... root meristems were excised and fixed in 4% formaldehyde (Polyscience) in PBS pH 7.2 for 24 h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... they were embedded in Spurr resin (Polyscience) and thermally polymerized ...
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bioRxiv - Cell Biology 2023Quote: ... Transient transfection of cells with plasmid DNA was performed on 80 % confluent cells using PEI-Max (Polyscience, cat.no. 24765-1, DNA:PEI ratio of 1:3) for 12 h ...
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bioRxiv - Cell Biology 2023Quote: ... we used polyethylenimine (PEI; Polyscience, 24765) dissolved in PBS to 1 mg/mL ...
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bioRxiv - Cell Biology 2023Quote: Samples were imaged in PBS (Polyscience) with 50 – 200 mM MEA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Ten µg of total DNA in 1 mL of plain optiMEM was mixed with 40 µl of Polyethyleneimine “Max” (PEI, Polyscience #24765, 1 mg/mL in dH2O), briefly vortexed and incubated at room temperature for 15 min ...
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bioRxiv - Biophysics 2023Quote: ... PEImax (PolyScience) was used to carry out transfection and was added as per the vendor’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... cells suspended in washing buffer were Concanavalin A coated magnetic beads (Polyscience, 86057) for 10 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... cultured at 27 ˚C with polyethylenimine MW 40,000 (PolyScience, 24765). After 5 days ...
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bioRxiv - Cell Biology 2023Quote: ... Other portions of livers were transferred to a 30% sucrose solution and frozen sections 5 µm think sections were prepared by the Histology Service of the Columbia University Molecular Pathology Core for staining with Oil Red-O (Polyscience #25962). Stained sections were photographed using a BX53 upright microscope attached to a DP72 digital camera (Olympus) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the rep-cap AAV8 helper plasmid and an adenoviral helper plasmid was mixed and transfected using polyethylenimine (PEI) (Polyscience, USA). The cells were harvested in 3 ml of lysis buffer and lysed by four freeze–thaw cycles 72 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Embedding was done using epoxy resin (PolyBed resin, Polyscience Inc., Warrington, PA, USA). Ultrathin sections were obtained ...
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bioRxiv - Immunology 2022Quote: ... or Omicron using PEI-MAX (Polyscience). Pseudovirus supernatants were collected approximately 72 hours post-transfection and used immediately or stored at -80°C ...
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bioRxiv - Immunology 2022Quote: ... The cultures were transfected using PEI-MAX (Polyscience) with cells grown to a density of 3.0 million cells per mL and cultivated for 3 days ...
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bioRxiv - Immunology 2022Quote: ... in the presence of 198 µl PEI (1mg/ml, PEI MAX, Polyscience). At 48 h after transfection ...