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Citations for PolyScience :
1 - 50 of 284 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... or pcDNA3.1-FLAG-hSF3B1-K700E and 0.5 µg of the respective minigene construct using the ratio 1:2 of DNA:PEI MAX (PolyScience, #24765-1) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells were transfection with the indicated spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... PEI (polyethylenimine 25 kDa linear, PolyScience) and DNA (3:1 ml PEI:mg DNA ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1.5 ug psPAX2 were mixed with PEI-MAX 40K (Polyscience) and transfected into seeded HEK293T in 6-well format ...
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bioRxiv - Molecular Biology 2024Quote: ... and immobilized with concanavalin A-coated magnetic beads (Polyscience # 86057) by rotating at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... DRG and spinal cord cross cryosections (20μm) were collected on glass slides (Superfrost Plus, Polyscience) and stored at −80°C until further processing ...
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bioRxiv - Molecular Biology 2024Quote: ... cell pellets were washed twice with sterile PBS and subjected to LR White (Polyscience, Inc., USA) embedding ...
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bioRxiv - Molecular Biology 2024Quote: 1.6 µg of expression plasmid DNA in 100 µl of 150 mM NaCl were mixed with 100 µl of 150 mM NaCl supplemented with 7 µl of 1 mg/ml PEI (polyethylenimine ‘MAX’ Polyscience, Inc.). After 30 min incubation at a room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... counterstained with DAPI (1:1000) and mounted with AquaPolymount (Polyscience). High-resolution images dendritic spines were acquired using a Nikon A1 Eclipse confocal microscope equipped with NIS element AR v4.20.03 software (Nikon ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 10% formalin (Polyscience, Cat# 08279-20). The brains were postfixed for 1hr with 10% formalin at r.t ...
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bioRxiv - Neuroscience 2024Quote: ... we used the PEI transfection reagent (Linear PEI25K, PolyScience; 23966-1). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... mounted in a 3 μL drop of M9 containing approximately fifty 25 μm diameter polystyrene beads (Polyscience Inc.) and sealed under a coverslip using Vaseline ...
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bioRxiv - Developmental Biology 2024Quote: ... elegans were mounted on 2% agarose (w/v in water) dry pads immersed in 2% polystyrene bead (0.1 mm diameter, Polyscience, 00876-15) diluted in M9 buffer ...
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bioRxiv - Immunology 2024Quote: ... 20,000 Polybead polystyrene Black Dyed Microspheres (Polyscience, Cat. 24293-15) were added to the samples prior to analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids containing shRNA or corresponding empty vector were cotransfected with lentivirus packaging plasmids (pLP1, pLP2, and pLP/VSVG) into HEK293T cells using linear polyethylenimine (23966, Polyscience) transfection method ...
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bioRxiv - Cancer Biology 2024Quote: ... 150 μl Concavalin-A slurry (Polyscience, 86057-3) per sample were activated by washing the bead slurry three times in 1.5 mL Binding Buffer (HEPES pH 7.5 20 mM ...
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bioRxiv - Biophysics 2024Quote: ... and a fresh 1:10,000 dilution of 1 µm beads (Polybead® Microspheres 1 µm, Polyscience, Inc) in medium was added to the sample ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293 cells were transfected with expression and packaging plasmids following standard calcium phosphate or polyethylenimine (Polyscience, 23966) protocols ...
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bioRxiv - Pathology 2024Quote: ... paraffin-embedded LV cross-sections of 5 µm were stained with picrosirius red (Polyscience Inc, Warrington, PA, USA) following standard protocols ...
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bioRxiv - Immunology 2024Quote: ... and pMD2.G (100 ng/well) plasmids using PEI MAX (Polyscience, 24765-100). Cells were incubated at 37C ...
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bioRxiv - Cell Biology 2024Quote: ... S2 cells (2 x 107) were washed and fixed in 1% formaldehyde (EM grade, Polyscience) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... DNA (1 µg/ml final transfection volume) was added followed by addition of linear polyethylenimine (PEI25k, Polyscience, Inc.) (1mg/ml ...
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bioRxiv - Immunology 2024Quote: ... The full length BD58-0730 and XBB.1.5 S6P were obtained by transient expression in the HEK293F cells using polyethylenimine (PEI, Polyscience). Culture supernatants were harvested at 96 h post transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... placed into an embedding mold (Polyscience), and transferred to the vacuum chamber ...
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bioRxiv - Biophysics 2024Quote: ... We transfected HeLa cells (ATCC CCL-2) with 10 μg of each px459 v2.0 plasmid together with 40 μl of linear PEI (Polyscience 23966) using standard protocols ...
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bioRxiv - Neuroscience 2024Quote: ... Core temperature was maintained through a water heating system (PolyScience, Niles, IL, USA) built into the surgical table ...
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bioRxiv - Neuroscience 2024Quote: ... and the packaging plasmid pCMVd8.9 (14.2 µg) into 293FT cells using 122 µg of transfection-grade linear polyethylenimine hydrochloride (PEI MAX; Polyscience Inc; Cat# 24765). The pVSV-G and pCMVd8.9 plasmids.93 After 3 hours ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were transiently transfected with plasmid DNA with the use of the PEI MAX reagent (Polyscience) and FuGENE HD (Promega) ...
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bioRxiv - Neuroscience 2024Quote: ... cells were mounted with Aqua-Poly Mount (Polyscience). All steps were carried at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... pHCMV-G VSV-G and pMDLg/pRRE together with the Brunello plasmid using polyethyleneimine (Polyscience Inc.). Virus was harvested ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 μg Pei-Max (Polyscience, Chemie Brunschwig) were added to the cells ...
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bioRxiv - Immunology 2024Quote: ... polystyrene nanoparticles (Polyscience Inc) were suspended in 2:1 mixture of water:ethanol ...
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bioRxiv - Immunology 2024Quote: Water bath (#WBE05A11B, PolyScience) (see Note 1)
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bioRxiv - Immunology 2024Quote: ... into HEK293T cells using polyethylenimine (PEI; Polyscience) for virus production ...
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bioRxiv - Cancer Biology 2024Quote: ... using polyethylenimine (PEI) (Polyscience, Cat # 23966-1) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected with pcDNA3.1-FLAG-LRRK2 and Rab10 plasmids with polyethylenimine hydrochloride (PEI, Polyscience, USA) and lysates harvested ∼16-hours later ...
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bioRxiv - Neuroscience 2024Quote: ... Counter staining with hematoxylin was performed prior to coverslipping with aqua polymount (Polyscience Inc., 18606-20). Hematoxylin and eosin staining was performed by the UT Southwestern Histopathology Core.
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were transfected 24 h after seeding with polyethyleneimine (PEI, 1 mg/ml in H2O, pH 7, Polyscience, catalog no. 23966-1). To do so ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells were transfected with 2µg of either pHTLV-1(p12stop) or pHTLV-1(CTCF) using 1mg/ml polyetheliamine (PEI40K, Polyscience) by a ratio of 3:1 plasmid concentration ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were seeded into multi-well plates and transfected with polyethyleneimine (PEI, 1 mg/mL in H2O, pH 7, Polyscience, catalog no. 23966-1). Therefore ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were placed in embedding molds (Polyscience) and hardened in a pre-warmed oven at 70°C for 72 hours.
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bioRxiv - Neuroscience 2024Quote: ... and mounted with Aqua Polymount (Polyscience Inc.).
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bioRxiv - Neuroscience 2024Quote: ... followed by 10% formalin (Polyscience, Cat# 08279-20). The brains were post-fixed for 1hr with 10% formalin at room temperature and then stored in PBS at 4°C until processed for immunohistochemistry.
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bioRxiv - Neuroscience 2024Quote: ... Core temperature was maintained through a water heating system (PolyScience, Niles, IL, USA) built into the surgical table ...
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bioRxiv - Developmental Biology 2024Quote: ... 90-μm polybead microspheres (Polyscience, Inc.) were placed between the slide and a No ...
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bioRxiv - Neuroscience 2024Quote: ... Plastics of three different sizes were used in this study - 50nm (Phosphorex, 2101) (Polyscience Inc, 17149-10), 500nm (Phosphorex ...
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bioRxiv - Physiology 2024Quote: ... diameter 1 µm with a coefficient of variation = 2%) labeled with a yellow-green fluorescent dye (excitation 441 nm and emission 485 nm) were obtained from Polyscience, Inc. ...
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bioRxiv - Cancer Biology 2024Quote: Plasmid transfection of 293A/T cells was performed using polyethylenimine (PEI) (23966-1, Polyscience) reagent 48 hours prior to lysis ...
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bioRxiv - Molecular Biology 2024Quote: ... pLenti V HELLS was co-transfected with psPAX2 and pMD2G plasmids into HEK293T cells using 3 µg/mL Polyethylenimine Max (Polyscience). 1 mL of virus supernatant was transduced with 8 µg/mL of polybrene (Millipore Sigma ...
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bioRxiv - Physiology 2024Quote: ... Picrosirius red staining was performed via manufacture protocol within the kit (Polyscience Inc, Cat# 24901). All histological samples were imaged using a Nikon Eclipse 90i fluorescent microscope with Nikon Digital Sight color DS-Fi1 camera ...